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Unilateral rNurr1-V5 transgene.expression in nigral dopamine.gic ne.rons mitigates bilateral ne.ropathology and behavioral deficits in parkinsonian rats withα-synucleinopathy

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ne.ral Regene.ation Research 2024年第9期19卷 2057-2067页

作者： Bismark Gatica-Garcia Michael J.Bannon irma Alicia.martíne.-dávila Luis O.Soto-Rojas david Reyes-Corona Lourdes Escobedo Mine.va Maldonado-Berny ME Gutierrez-Castillo Armando J.Espadas-Alvarez Manuel A.Fernandez-Parrilla Juan U.Mascotte-Cruz CP Rodríguez-Oviedo Irais E.Valenzuela-Arzeta Claudia Luna-Herrera Francisco E.Lopez-Salas Jaime Santoyo-Salazar daniel martine.-Fong departamento de Fisiología Biofísica y NeurocienciasCentro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico department of Pharmacology Wayne State University School of MedicineDetroitMIUSA Laboratorio de Patogéne.is Molecular Carrera Médico CirujanoFacultad de Estudios Superiores IztacalaUniversidad Nacional Autónoma de MéxicoTlalnepantla de BazMéxico Red de Medicina para la Educación y desarrollo y la Investigación Científica de Iztacala(Red MEdICI) Carrera Médico CirujanoFacultad de Estudios Superiores IztacalaUniversidad Nacional Autónoma de MéxicoTlalnepantla de BazMéxico Nanoparticle Therapy Institute AguascalientesMéxico departamento de Biociencias e Ingeniería Centro Interdisciplinario de Investigaciones y Estudios sobre Medio Ambiente y DesarrolloInstituto Politécnico NacionalCiudad de MéxicoMéxico Escuela Nacional de Medicina y Homeopatía Instituto Politécnico NacionalCiudad de MéxicoMéxico departamento de Fisiología Escuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalCiudad de MéxicoMéxico departamento de Biología Molecular y Biotecnología Instituto de Investigaciones BiomédicasUniversidad Nacional Autónoma de MéxicoCiudad de MéxicoMéxico departamento de Física Centro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico Programa de Nanociencias y Nanotecnología Centro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico

Parkinsonism by unilateral,intranigralβ-sitosterolβ-d-glucoside administration in rats is distinguished in that theα-synuclein insult begins unilaterally but spreads bilaterally and increases in severity over time,thus replicating several clinical features of Parkinson’s disease,a typicalα-*** Nurr1 repressesα-synuclein,we evaluated whether unilateral transfected of rNurr1-V5 transgene.via ne.rotensin-polyplex to the substantia nigra on day 30 after unilateralβ-sitosterolβ-d-glucoside lesion could affect bilateral ne.ropathology and sensorimotor deficits on day 30 *** study found that rNurr1-V5 expression but not that of the green fluorescent protein(the ne.ative control)reducedβ-sitosterolβ-d-glucoside-induced ***,a bilateral increase in tyrosine.hydroxylase-positive cells and arborization occurred in the substantia nigra and increased tyrosine.hydroxylase-positive ramifications in the *** addition,tyrosine.hydroxylase-positive cells displayed less sene.cence markerβ-galactosidase and more ne.ron-cytoskeleton markerβIII-tubulin and brain-derived ne.rotrophic factor.A significant decrease in activated microglia(positive to ionized calcium-binding adaptor molecule 1)and ne.rotoxic astrocytes(positive to glial fibrillary acidic protein and complement compone.t 3)and increased ne.rotrophic astrocytes(positive to glial fibrillary acidic protein and S100 calcium-binding protein A10)also occurred in the substantia *** effects followed the bilateral reduction inα-synuclein aggregates in the nigrostriatal system,improving sensorimotor *** results show that unilateral rNurr1-V5 transgene.expression in nigral dopamine.gic ne.rons mitigates bilateral ne.rodegene.ation(sene.cence and loss of ne.ron-cytoskeleton and tyrosine.hydroxylase-positive cells),ne.roinflammation(activated microglia,ne.rotoxic astrocytes),α-synuclein aggregation,and sensorimotor *** ne.rotrophic astrocytes and

关键词： A1 astrocytes A2 astrocytes gene.therapy microglia motor deficits nanoparticles ne.rodegene.ation ne.roinflammation sene.cence α-synuclein aggregates

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Nitrogen-doped Amorphous Carbon Homojunction from Palmyra Sugar as a Rene.able Solar Cell

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Journal of Rene.able Materials 2024年第1期12卷 57-69页

作者： Budhi Priyanto Imam Khambali irma Septi Ardiani Khoirotun Nadhiyah Anna Zakiyatul Laila M.Chasrun Hasani Bima Romadhon Retno Asih Yoyok Cahyono Triwikantoro darminto department of Electronic Engine.ring Faculty of EngineeringUniversitas Muhammadiyah MalangKota MalangIndonesia Advanced Materials Research Group Department of PhysicsFaculty of Science and Data AnalyticsInstitut Teknologi Sepuluh NopemberSurabayaIndonesia department of Electronic Engine.ring Faculty of Industrials EngineeringInstitut Teknologi National MalangKota MalangIndonesia

An a-C/a-C:N junction,which used palmyra sugar as the carbon source and ammonium hydroxide(NH4OH)as the dopant source,was successfully deposited on the ITO glass substrate using the nano-spraying *** current-voltage relationship of the junction was found to be a Schottky-like contact,and therefore the junction shows the characteristic *** means the a-C and a-C:N are semiconductors with different types of ***,the samples showed an increase in current and voltage value when exposed to visible light(bright state)compared to the dark condition,thereby,indicating the creation of electron-hole pairs during the *** was also discovered that the relationship between current and voltage for the a-C/a-C:N junction sample formed a curve that satisfies the rule of the photovoltaic effect when exposed to visible light from a light *** exposure of this sample to direct sunlight at AM 1.5 conditions produced a curve that meets the rules for the emergence of the photovoltaic effect with higher characteristics for the current-voltage ***,the a-C/a-C:N junction sample is a solar cell successfully fabricated using a sample method and has a maximum efficiency of 0.0013%.

关键词： Solar cell amorphous carbon palmyra sugar

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Cerebral dopamine.ne.rotrophic factor transfection in dopamine.ne.rons using ne.rotensin-polyplex nanoparticles reverses 6-hydroxydopamine.induced nigrostriatal ne.rodegene.ation

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ne.ral Regene.ation Research 2022年第4期17卷 854-866页

作者： Manuel A.Fernandez-Parrilla david Reyes-Corona Yazmin M.Flores-martine. Rasajna Nadella Michael J.Bannon Lourdes Escobedo Mine.va Maldonado-Berny Jaime Santoyo-Salazar Luis O.Soto-Rojas Claudia Luna-Herrera Jose Ayala-davila Juan A.Gonzalez-Barrios Gonzalo Flores Maria E.Gutierrez-Castillo Armando JEspadas-Alvarez irma a.martíne.-dávila Porfirio Nava daniel martine.-Fong departamento de Fisiología Biofísica y NeurocienciasCentro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico Programa Institucional de Biomedicina Molecular Escuela Nacional de Medicina y HomeopatíaInstituto Politécnico NacionalCiudad de MéxicoMéxico department of Biosciences IIIT-SrikakulamRajiv Gandhi University of Knowledge Technologies(RGUKT)Andhra PradeshIndia department of Pharmacology Wayne State University School of MedicineDetroitMIUSA departamento de Física Centro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico Facultad de Estudios Superiores Iztacala Universidad Nacional Autónoma de MéxicoTlalnepantla de BazEdo.de MéxicoMéxico departamento de Fisiología Escuela Nacional de Ciencias BiológicasCiudad de MéxicoMéxico Laboratorio de Medicina Genómica Hospital Regional“1°de Octubre”ISSSTECiudad de MéxicoMéxico Laboratorio de ne.ropsiquiatría Instituto de FisiologíaBenemérita Universidad Autónoma de PueblaPueblaPueblaMéxico departamento de Biociencias e Ingeniería Centro Interdisciplinario de Investigaciones y Estudios sobre Medio Ambiente y DesarrolloInstituto Politécnico NacionalCiudad de MéxicoMéxico Programa de Nanociencias y nanotecnología Centro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico

Overexpression of ne.rotrophic factors in nigral dopamine.ne.rons is a promising approach to reverse ne.rodegene.ation of the nigrostriatal dopamine.system,a hallmark in Parkinson's *** human cerebral dopamine.ne.rotrophic factor(h CdNF)has recently emerged as a strong candidate for Parkinson's disease *** study shows that h CdNF expression in dopamine.ne.rons using the ne.rotensinpolyplex nanoparticle system reverses 6-hydroxydopamine.induced morphological,biochemical,and behavioral *** independent electron microscopy techniques showed that the ne.rotensin-polyplex nanoparticles containing the h CdNF gene.ranging in size from 20 to 150 nm,enabled the expression of a secretable h CdNF in *** injection in the substantia nigra compacta on day 21 after the 6-hydroxydopamine.lesion resulted in detectable h CdNF in dopamine.ne.rons,whose levels remaine. constant throughout the study in the substantia nigra compacta and *** with the lesione. group,tyrosine.hydroxylase-positive(TH^(+))nigral cell population and TH+fiber density rose in the substantia nigra compacta and striatum after h CdNF *** increase inβIII-tubulin and growth-associated protein 43 phospho-S41(GAP43 p)followed TH^(+)cell recovery,as well as dopamine.and its catabolite *** reversal(80%)of drugactivated circling behavior and full recovery of spontane.us motor and non-motor behavior were ***-derived ne.rotrophic factor recovery in dopamine.ne.rons that also occurred suggests its participation in the ne.rotrophic *** findings support the potential of nanoparticle-mediated h CdNF gene.delivery to develop a disease-modifying treatment against Parkinson's *** Institutional Animal Care and Use Committee of Centro de Investigación y de Estudios Avanzados approved our experimental procedures for animal use(authorization No.162-15)on June.9,2019.

关键词： axonal growth brain-derived ne.rotrophic factor gene.delivery nanoparticles ne.ritogene.is ne.ronal cytoskeleton ne.roregene.ation ne.rorestoration ne.rotrophic therapy Parkinson's disease reinne.vation substantia nigra

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Oxidative stress,the blood-brain barrier and ne.rodegene.ative diseases:The critical bene.icial role of dietary antioxidants

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Acta Pharmaceutica Sinica B 2023年第10期13卷 3988-4024页

作者： Jin Feng Youle Zheng Mingyue Guo irma Ares marta.martíne. Bernardo Lopez-Torres María-Rosa.martíne.-Larran aga Xu Wang Arturo Anadon María-Aranzazu martíne. National Reference Laboratory of Veterinary drug Residues(HZAU)and MAO Key Laboratory for detection of Veterinary drug Residues Huazhong Agricultural UniversityWuhan 430070China MAO Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products Huazhong Agricultural UniversityWuhan 430070China department of Pharmacology and Toxicology Faculty of Veterinary MedicineUniversidad Complutense de Madrid(UCM)And Research Institute Hospital 12 de Octubre(i+12)Madrid 28040Spain

In recent years,growing awarene.s of the role of oxidative stress in brain health has prompted antioxidants,especially dietary antioxidants,to receive growing attention as possible treatments strategies for patients with ne.rodegene.ative diseases(Nds).The most widely studied dietary antioxidants include active substances such as vitamins,carotenoids,flavonoids and *** antioxidants are found in usually consumed foods such as fresh fruits,vegetables,nuts and oils and are gaining popularity due to recently growing awarene.s of their potential for preventive and protective agents against Nds,as well as their abundant natural sources,gene.ally non-toxic nature,and ease of long-term *** review article examine. the role of oxidative stress in the development of Nds,explores the‘two-sidedne.s’of the blood-brain barrier(BBB)as a protective barrier to the ne.vous system and an impeding barrier to the use of antioxidants as drug medicinal products and/or dietary antioxidants supplements for prevention and therapy and reviews the BBB permeability of common dietary antioxidant suplements and their potential efficacy in the prevention and treatment of ***,current challenges and future directions for the prevention and treatment of Nds using dietary antioxidants are discussed,and useful information on the prevention and treatment of Nds is provided.

关键词： dietary antioxidant supplements Oxidative stress Blood-brain barrier ne.rodegene.ative diseases Vitamins Carotenoids Flavonoids Polyphenols

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Lactase non-persistence and milk consumption in Estonia

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World Journal of Gastroenterology 2006年第45期12卷 7329-7331页

作者： Margus Lember Suvi Torniaine. mart Kull Riina Kallikorm Peeter Saadla Tarvo Rajasalu Hanna Komu irma Jrvel department of Internal Medicine University of Tartu Tartu Estonia department ofMedical Gene.ics University of Helsinki Helsinki Finland department of Medical Gene.ics University of Helsinki Helsinki Finland Laboratory of Molecular gene.ics Helsinki University Central Hospital Helsinki Finland

AIM： To define.the frequency of the cfr-13910 variant associated with lactase persistence/non-persistence trait and to analyze the milk consumption of lactase non- persistent subjects in Estonia. METHOdS： We genotyped 355 Estonians by polymerase chain reaction and direct sequencing, Milk consumption was analyzed by a questionnaire, specially developed to analyze milk consumption and abdominal complaints. RESULTS： The frequency of the genotype of the C/ C-13910 （lactase non-persistence） was found to be 24.8% in native Estonians. No other single nucleotide polymorphisms covering the region of 400 bp adjacent to the C/T-13910 variant were found. Lactase non- persistence subjects were found to consume less milk than lactase persistence subjects. CONCLUSION： The frequency of lactase non- persistence define. by the C/C-13910 genotype confirms the results of the previous studies based on indirect methods of determining hypolactasia, Milk consumption of lactase non-persistence subjects is consistent with previously reported figures of adult-type hypolactasia in Estonia, However, lactase non-persistence does not prevent the intake of milk in many adults.

关键词： Lactase persistence Milk Estonia

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Phenotypical Aspects of a Familial Syndromic Retinitis Pigmentosa

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Open Journal of Ophthalmology 2024年第2期14卷 168-173页

作者： irma Ene.da dos Santos Joseph Matar Mass Ndiaye Mariama diambone.Badji Alioune.Abdoulaye Ndongo Gerauld Akpo Jean Pierre diagne.Gabriel Karold Mendy Mouhamed Attye Aboubacry Sadikh Sow Elhadji Amadou Ba Paule Aida Ndoye Roth Ophthalmology department of Aristide Le dantec Hospital Dakar Senegal Pediatric department of Aristide Le dantec Hospital Dakar Senegal Radiological Imaging department of Aristide Le dantec Hospital Dakar Senegal Ophthalmology department of Abass Ndao Hospital Dakar Senegal

Aim: To report a familial case of syndromic retinitis pigmentosa identified at Aristide Le dantec Hospital in dakar and to describe their clinical characteristics ophthalmic. Observation: We report a sibling group of nine.children, four died at a young age from unknown causes. Three children were affected by retinitis pigmentosa, two cases were syndromic. A history of nyctalopia was found in all three affected children. The mean age of onset of decreased visual acuity was 6.6 years. Patient 1 affected by syndromic retinitis pigmentosa had an extraocular sign of cystic dilation of the main bile duct. Patient 2 had myoclonic epilepsy, psychomotor retardation, and the molar tooth sign on cerebral MRI (highly suggestive of Joubert syndrome). The third child had isolated retinitis pigmentosa. Ophthalmological examinations (fundus examination, electroretinogram, and visual evoked potentials) and pediatric examinations in the remaining two children were normal. discussion and Conclusion: Retinitis pigmentosa is a rare degene.ative disease that can be associated with several other malformations, highlighting the importance of screening for associated conditions. It presents a grim functional prognosis and a life prognosis dependent on extraocular manifestations. Molecular biology (karyotyping, ne.t-gene.ation sequencing) could have identified the implicated gene. and allowed for a formal diagnosis and gene.ic counseling.

关键词： Retinitis Pigmentosa Syndromic Heredity Ciliopathy

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Reduction of serum level of interleukin-2 and pruritus severity after acupuncture at Quchi(LI11)in hemodialysis patients:a placebo-controlled randomized clinical trial

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Journal of Acupuncture and Tuina Science 2022年第2期20卷 126-133页

作者： dedi ARdINATA Rozaimah ZAIN-HAMId irma d MAHAdI Hasan MIHARdJA department of Physiology Faculty of MedicineUniversitas Sumatera UtaraMedan20155Indonesia department of Pharmacology and Therapeutic Faculty of MedicineUniversitas Sumatera UtaraMedan20155Indonesia department of dermatology and Vene.eology Faculty of MedicineUniversitas Sumatera UtaraMedan20155Indonesia department of Medical Acupuncture Faculty of MedicineUniversitas IndonesiaJakarta10430Indonesia

Objective To observe the effect of acupuncture at Quchi(LI11)on the serum interleukin-2(IL-2)level and the severity of uremic pruritus(UP)in hemodialysis *** Sixty hemodialysis patients with UP were randomized into an acupuncture group and a control group,with 30 cases in each *** the acupuncture group,patients were treated with acupuncture at Quchi(LI11)twice a week for six *** the control group,each patient received a placebo treatment(sham acupuncture).Before and after the intervention,the pruritis severity was examine. using a 5-d scale,and the serum IL-2 level was measured using an enzyme-linked immunosorbent assay *** There were no statistically significant differences in the demographic or clinical factors between the control group and the acupuncture group at *** reduction in pruritus severity in patients was more significant in the acupuncture group(P=0.027).Acupuncture therapy also significantly decreased the serum IL-2 level in the patients(P=0.011).Throughout the acupuncture procedures,the incidence of pain and mild bleeding was 3.6%and 2.5%,*** Six-week acupuncture treatment at Quchi(LI11)can effectively decrease the severity of UP and the serum IL-2 ***-scale,long-duration,and multi-center studies are ne.ded to fully understand the role of IL-2 in inflammation and ne.roimmune.stimulation during acupuncture.

关键词： Acupuncture Therapy Point Quchi (LI11) Interleukin-2 Pain Pruritus Hemodialysis Randomized Controlled Trial

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Geothermal diatoms: Seasonal Variability in the El Tatio Geothermal Field (Altiplano, Chile)

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Advances in Microbiology 2018年第3期8卷 211-234页

作者： Alejandro Angel irma vila Carolina díaz Ximena Molina Paola Sepúlveda Faculty of Science Universidad de Chile Santiago Chile Laboratorio Ambiental Centro de Ecología Aplicada Santiago Chile

diatom floras were examine. in a high-altitude geothermal field, 4200 - 4500 m (29°19'S 68°W'), located in the Central Andean dry Puna ecoregion or southern Altiplano. These locations include hostile environments subjecting living organisms to extreme conditions. The aim of the present study was to investigate the spatial patterns and describe the response of diatom assemblages to differences in physical and chemical variables. different shallow (Achnanthidium exiguum (Grunow) Czarne.ki, Coccone.s placentula var. line.ta (Ehrenberg) Van Heurck, Eolimna minima (Grunow) Lange-Bertalot, Staurosirella pinnata (Ehrenberg) Williams and Round, Navicula gregaria donkin, Nitzschia inconspicua Grunow, Nitzschia palea (Kützing) Smith, Nitzschia perminuta (Grunow) Peragallo, and Planothidium lanceolatum (Brébisson ex Kützing) Lange-Bertalot. As expected, the 20 to 200 μm-size fraction containe. the highest numbers of diatom taxa (53 species), although an une.pectedly high number (47 species) were also found in the smaller 5 to 20 μm-size fraction, more associated to fumaroles and saline.systems. The 180 to 2000 μm size fraction containe. only two species, including rosette-forming diatom Ulnaria ulna (Nitzsch) Compère, and the unicellular species Surirella chilensis Janisch, both species exclusively reported in freshwater systems. Canonical correspondence analysis (CCA) and Monte Carlo permutation tests showed clear correlations between species, conductivity, TP (total phosphorous), NO3- , HO3- , Mg2+, temperature and dissolved oxygen (dO). The gradient of ionic composition values explaining most variation in diatom assemblages ranged from waters dominated by Ca2+ and SO4- to waters with higher proportions of Na+, K+, F-, Li+, Mg2+ and Cl-. Other factors include substrate type, presence of macrophytes, current velocity and other local environmental conditions. The results presented here enhance our understanding of diatom richne.s/composition in hostile environments from a high

关键词： Canonical Correspondence Analysis Cluster Conductivity Fumaroles Size Fraction

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Georgian Literature before the Weltliteratur

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Journal of Foreign Languages and Cultures 2023年第2期7卷 16-24页

作者： irma Ratiani Iv.Javakhishvili Tbilisi State University

The history of Georgian writing starts much earlier than when Goethe introduced the term"Weltliteratur."It starts from the era of Christianity from the 4th *** to the fast spread of Christianity in the Early Medieval period,Georgia was already included in the European ne. of Christian *** branches of Christian spiritual literature were *** culture and literature naturally were developing in the frame of the Western European *** period of the 11th-12th centuries was a Golden Era for Georgia,and the heyday of fame for Georgian culture and literature as *** during this period,"The Knight in the Panther's Skin"was created by Shota *** from its aesthetic,philosophical,and worldview depth,it is a first text in Georgian literature as well as in European literature which reflects the clashing of two huge universes in Georgian culturethe West and the *** Western principles are revealed in the Christian worldview of the text,in the way of thinking of the author and in its genre;however,the 12th century is already a period of strengthening of the influence of Eastern culture and literature in the European part of the Caucasus,and Rustaveli regards with obvious favor the Oriental poetic ***,at this stage of European literary history,Georgian literature was separated from the Western European literary process due to tragic political *** for literature,it was a period of almost three centuries of *** the fall of Constantinople,Georgian literature had to move closer to the Eastern area as an historically offered *** the 17th century,the process of the returning of Georgian political and cultural life back within the European frame had been *** Georgian writers were able to tie Georgian literature to the cultural models of European Classicism and the Enlightenment.

关键词： Georgian literature European literature the West and the East

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Bacterial diversity in Fez tanne.ies and Morocco's Binlamdoune.River,using 16S RNA gene.based fingerprinting

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Journal of Environmental Sciences 2010年第12期22卷 1944-1953页

作者： Adil Essahale Moustafa Malki irma Marín Mohieddine.Moumni Moulay Isml University Faculty of ScienceDepartment of BiologyBP 11 201 ZitouneMeknès 50000Morocco department of Molecular Biology Faculty of ScienceAutonomous University of Madrid (UAM)

Tanne.y wastewater causes serious ecological and sanitary damage. Chemical analysis of water from Binlamdoune.River of the medina of Fez was conducted and the results revealed the presence of toxic elements from tanne.ies and other industrial activities, which strongly affected water quality. To determine.the effectivene.s of bioremediation for depollution, we studied the abundance and diversity of bacteria residing in these polluted environments. Conducting denaturing gradient gel electrophoresis （PCR-dGGE） of the 16S rdNA area using primers related to bacteria showed a bacterial community belonging to eubacterial groups, that is, Epsilonproteobacteria, Clostridia, Lactobacillales, Bacteroidetes, Gammaproteobacteria, and Alphaproteobacteria. In addition, cloning displayed the presence of clone. belonging to the Firmicutes group. Moreover, scanning electron microscopy revealed a significant heterogene.ty of microorganism forms and structures. These endogenous microbes could have a significant role in the purification of Binlamdoune.River and Fez tanne.y wastewater.

关键词： Fez tanne.ies Binlamdoune.River biodiversity bacteria 16S rRNA

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