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American Journal of Plant Sciences 2015年第19期6卷 3231-3243页

作者： Ahou Anique Gbotto Kouamé Kevin Koffi Jean-Pierre Baudoin irié arsène zoro bi Laboratoire de Génomique Fonctionnelle et Amélioration Génétique (LaGeFAGe) UFR-SN Université Nangui Abrogoua Abidjan C&ocirc te d’Ivoire Unité de Phytotechnie Tropicale et d’Horticulture Gembloux Agro Bio Tech Université de Liège Gembloux Belgium

Thirty accessions of Lagenaria siceraria from the Nangui Abrogoua University germplasm collection were analyzed using three microsatellite markers. The average Polymorphism Information Content (PIC) value was 0.61. The average observed heterozygosity value (H0 = 0.631) compared to the average expected heterozygosity value (He = 0.645) did not show significant differences in the selected accessions, which suggested random mating in the set of accessions. Within accession inbreeding estimate (FIS), was 40% and was not significantly different from zero. The reduction of heterozygotes was likely the result of presence of null alleles. Analysis of Molecular Variance (AMOVA) within and among 30 accessions of L. siceraria revealed that 39% of the total variation resides among accessions and 61% within accessions. Accession structuring pattern derived from Bayesian clustering analysis revealed two clusters. Based on the genetic structure of the accessions analyzed, a sampling strategy to collect and conserve genetic resources of L. siceraria was suggested.

关键词： Genetic Structure Lagenaria siceraria Null Alleles Oleaginous SSR

Medium effects on micropropagation and genetic stability of Citrullus lanatus oleaginous type

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Agricultural Sciences 2013年第7期4卷 32-44页

作者： Yah Gwladys Gnamien irié arsène zoro bi Yatty Justin Kouadio Yves Brostaux Jean-Pierre Baudoin Laboratory of biology and Improving Vegetable Productions Natural Sciences UnitUniversity Nangui Abrogoua(UNA)AbidjanCote d’Ivoire Tropical Crop Husbandry and Horticulture Gembloux Agro-Bio Tech(GxABT)University of LiègeGemblouxBelgium Department of Applied Statistics Computer Science and MathematicsGembloux Agro-Bio Tech(GxABT)University of LiègeGemblouxBelgium

To regenerate adventitious shoots from the cotyledon proximal parts of Citrullus lanatus (Thunb.) Matsum. and Nakai ssp. mucosospermus (Fursa) oleaginous type, different concentrations of MS mineral elements, sucrose, 6-benzylaminopurine (BAP) and agar were tested. Shoot induction proved to depend on the interaction between levels of sucrose, BAP and MS mineral elements in the medium. The medium containing 3/2 strength of MS mineral elements, 35 g/l sucrose and 1 mg/l BAP solidified with 6 g/l agar allowed the production of numerous shoots without a callus phase. After 3 weeks of culture, 76.7% of the cotyledon proximal parts induced shoots with an average of 12.26 shoots per explant and a mean shoot length of 17.13 mm. The induced shoots were directly rooted and thus complete plants ready for acclimatization were obtained using a two steps procedure. Depending on the genotype, the shoot induction from cotyledon proximal parts ranged from 54% to 96%. Rooted plantlets were acclimatized and transferred to field, where they grew well, developed flowers and fruits like seeded plants. The assessment of the genetic stability of the in-vitro-regenerated plantlets by means of an Amplified Fragment Length Polymorphism (AFLP) analysis with the combination of 5 primers revealed no differences between regenerated plantlets and mother plants.

关键词： AFLP Genetic Stability In Vitro Oleaginous Seeds Organogenesis

Enhancing Tomato (Solanum lycopersicum L.) Fruit Yield and Quality and Blossom End Rot Control Using Different biological Calcium Sources

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Journal of Agricultural Chemistry and Environment 2023年第3期12卷 263-274页

作者： Alain Serge Coulibaly Kouakou Laurent Kouakou Jonas Patrick Dao Camille Kouakou Juliette Ky Dedi irié arsène zoro bi Training and Research Unit of Sciences of Nature Laboratory of Biology and Improvement of Plant Production Nangui Abrogoua University Abidjan C&ocircte d’Ivoire Phytotechnical Unit and Genetic Improvement Training and Research Unit of Sciences of Nature Nangui Abrogoua University Abidjan C&ocircte d’Ivoire Training and Research Unit of biosciences Plant Physiology Laboratory Felix Houphou&eumlt Boigny University Abidjan C&ocircte d’Ivoire

Cultivated for its richness in nutrients, tomato culture is demanding fertilizer, especially in calcium. Calcium deficiencies and physiological disorders cause serious diseases in tomatoes. Blossom end rot (BER), in susceptible cultivars, may cause severe economic losses. The goal of this study is to determine the effect of three organic calcium sources on the growth and quality of fruit production of tomatoes. Egg, snail and sea shell powders and extracts have been used as biological fertilizers. The experiment was conducted in a block factorial trial with three replications. The trials comprised two factors: calcium sources and calcium source form (powder and extract). The results showed that the growth parameters and yield were improved by the calcium source, whatever the form. Also, BER was lower in plants treated with calcium contrary to the control. However, plants treated with eggshells showed the best growth (mean) and the lowest rate (0.40%) of BER than the snail and sea shell.

关键词： Tomatoes Blossom End Rot Eggshells Snail Shells Seashells

Effect of Leafy and Leafless Greenwood, Softwood and Hardwood Cuttings Success of <i>Garcinia kola</i>(Heckel)

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Agricultural Sciences 2020年第10期11卷 897-911页

作者： Jonas Patrick Dao Kouakou Laurent Kouakou Camille Kouakou Mamadou Cherif Mahamadi Hamed Ouedraogo Kouame Kevin Koffi irié arsène zoro bi Training and Research Unit of Sciences of Nature Laboratory of Biology and Improvement of Plant Production Nangui Abrogoua University Abidjan C&#244 te d’Ivoire Training and Research Unit of biosciences Plant Physiology Laboratory Felix Houphou&#235 t Boigny University Abidjan C&#244 te d’Ivoire Phytotechnical Unit and Genetic Improvement Training and Research Unit of Sciences of Nature Nangui Abrogoua University Abidjan C&#244 te d’Ivoire Training and Research Unit of Life and Earth Sciences Biosciences Laboratory Genetics and Plant Breeding Joseph Ki-Zerbo University Ouagadougou Burkina Faso

Garcinia kola Heckel, called “petit cola” in Côte d’Ivoire and “bitter kola” in Nigeria, is a Non-Timber Forest Product of great socioeconomic importance. Unfortunately, this species is threatened due to overexploitation and deforestation. This study tested the effect of leaf area on stem cuttings to regenerate vegetatively in a non-mist poly-propagator without using exogenous hormones. Three stem cuttings types: greenwood, softwood and hardwood (12 - 15 cm in length) were collected from 24-month years old seedlings. The two top leaves of each cutting type were conserved at different leaf area: 0 cm2, 28.25 cm2, 56.5 cm2, 84.75 cm2 and 113 cm2 (entire leaf area). Observations were made on rooting, sprouting and leafing abilities of cuttings depending to treatments applied. Results showed that all cutting types have presented good ability to shooting and rooting. Cuttings with 113 cm2 leaf areas presented the best rooting (94.44% ± 2.42%) and shouting (95.55% ± 2.42%) percentage and longest primary root length (12.03 ± 0.50 cm). Hardwood, softwood and greenwood cuttings with 113 cm2 leaf area had the best rooting percentage (96.66% ± 3.33%, 96.66% ± 5.77% and 90.00% ± 5.77% respectively). The longest primary root length (13.50 ± 0.97 cm) was observed to greenwood cuttings with 113 cm2 leaf area. Regenerated plants in the forest presented well survival percentage (96.66%). This study shows that it is possible to regenerate entire plants by cuttings without using external hormones in non-mist poly-propagator.

关键词： Garcinia kola Heckel Leaf Area Non-Mist Poly-Propagator Stem Cuttings Vegetative Propagation

biometric Analysis on Genetic Divergence between Parental and Regenerated Accessions in Tall Coconut Palms (Cocos nucifera L.) from International Genebank for Africa and the Indian Ocean

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Open Journal of Genetics 2022年第2期12卷 11-23页

作者： Saraka Didier Martial Yao Wentoin Alimata Marie Pierre Daramcoum Eric-Blanchard Zadjéhi Koffi Konan Jean Louis Konan Nafan Diarrassouba Bourdeix Roland Raoul Sylvère Sie irié arsène zoro bi Department of biochemistry and Genetics Pedagogical and Research Unit (UPR) of Genetics Shea Breeding Program University of Peleforo Gon Coulibaly (UPGC) Korhogo C&#244 te d’Ivoire Centre National de Recherche Agronomique (CNRA) Marc Delorme Research Station of Port-Bou&#235 t Abidjan C&#244 te d’Ivoire CEFE-CNRS Montpellier France Department of Natural Science Nangui Abrogoua University Abidjan C&#244 te d’Ivoire

This study endeavour assesses agromorphological likeness between initial introductions and regenerated accessions at the International Coconut Genebank for Africa and the Indian Ocean (ICG-AIO) based in C?te d’Ivoire. Ten couples of parental (G0) and regenerated (G1) accessions of Tall coconut palms were analyzed using Principal Component Analysis (PCA) and Multiple Analysis of Variance (MANOVA) from 26 agromorphological characters. The main results showed a relative decrease in the expression of the phenotypical traits concerning the component of the fruit, height and vigor of the stem and yield of bunches and fruits after one regeneration cycle. But, a high proportion (69%) of studied characters from leaf, inflorescence and nut components showed likeness between G0 and G1 accessions. After one regeneration cycle, the controlled pollination method guarantees significant conservation of the expression of the majority of agromorphological traits. Consequently, regenerated accessions of Tall coconut palms can be used to pursue research and development programs in C?te d’Ivoire.

关键词： Agromorphological Likeness Controlled Pollination Côte d’Ivoire Regenerated Accessions Tall Coconut

On the Proper Holomorphic Mappings between Equidimensional Hartogs Domains over Hermitian Symmetric Manifolds

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Acta Mathematica Sinica,English Series 2024年第5期40卷 1215-1228页

作者： En Chao bi School of Mathematics and Statistics Qingdao UniversityQingdao 266071P.R.China

In this paper,we study a family of Hartogs domains fibred over Hermitian symmetric manifolds being a unit ball in C^(m).The aim of the present study is to establish the rigidity results about proper holomorphic mappings between two equidimensional Hartogs domains over Hermitian symmetric *** particular,we can fully determine its biholomorphic equivalence and automorphism group.

关键词： Proper holomorphic mapping Hartogs domains Hermitian symmetric manifolds

Advances in regional nodal management of early-stage breast cancer

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Chinese Journal of Cancer Research 2024年第2期36卷 215-225页

作者： Zhao bi Yongsheng Wang Breast Cancer Center Shandong Cancer Hospital and InstituteShandong First Medical University and Shandong Academy of Medical SciencesJinan 250017China

With the continuous improvement of systemic treatment, reasonable local regional control of early-stage breast cancer can be translated into survival benefits. The optimization of regional nodal management in patients with limited sentinel lymph node(SLN) metastasis needs to be weighed by surgical complications, regional recurrence risk, and lymph node status, as well as other escalating treatment(systemic/radiotherapy) that may result from deescalating surgery. With the effective support and supplementation of systemic therapy and radiotherapy, the management of axillary surgery is developing in a de-escalating trend. The widespread application of neoadjuvant therapy has contributed to optimizing the management of patients with clinically node-negative/imaging nodepositive disease. In clinical practice, it is necessary to consider the residual tumor burden of regional lymph nodes when formulating the optimal irradiation fields in patients with limited positive SLN without axillary lymph node dissection. The combined application of genomic tests and American College of Surgeons Oncology Group Z0011/AMAROS criteria could provide patients with a better strategy of dual de-escalation treatment, which includes the de-escalation of both axillary surgery and systemic treatment. In the era of sentinel lymph node biopsy(SLNB), the regional nodal management of breast cancer should adhere to the concept of “updating ideas, making bold assumptions, and carefully seeking proof”, make full use of the benefits of systemic therapy and radiotherapy to reduce the scope of surgery and complications, and expand the “net benefit” of efficacy and quality of life. This review discusses the optimization of regional nodal management in the era of SLNB, in order to provide reference information for clinicians.

关键词： Breast cancer sentinel lymph node biopsy internal mammary lymph node radiotherapy surgery

On-the-fly precision spectroscopy with a dual-modulated tunable diode laser and Hz-level referencing to a cavity

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Advanced Photonics 2024年第4期6卷 36-44页

作者： Shuangyou Zhang Toby bi Pascal Del’Haye Max Planck Institute for the Science of Light Friedrich-Alexander Universit?t Erlangen-Nürnberg Department of Physics

Advances in laser spectroscopy have enabled many scientific breakthroughs in physics, chemistry,biology, and astronomy. Optical frequency combs pushed measurement limits with ultrahigh-frequency accuracy and fast-measurement speed, while tunable-diode-laser spectroscopy is used in scenarios that require high power and continuous spectral coverage. Despite these advantages of tunable-diode-laser spectroscopy, it is challenging to precisely determine the instantaneous laser frequency because of fluctuations in the scan speed. Here, we demonstrate a simple spectroscopy scheme with a frequencymodulated diode laser that references the laser on-the-fly to a fiber cavity. The fiber cavity's free spectral range is on-the-fly calibrated with sub-10-Hz frequency precision. We achieve a relative precision of the laser frequency of 2 × 10-8for an 11-THz frequency range at a measurement speed of 1 THz∕s. This is an improvement of more than 2 orders of magnitude compared to existing diode-laser-spectroscopy *** scheme provides precise frequency calibration markers, while simultaneously tracking the instantaneous scan speed of the laser. We demonstrate the versatility of our method through various applications, including dispersion measurement of a fiber, ultrahigh-Q microresonators, and spectroscopy of a hydrogen fluoride gas cell. The simplicity, robustness, and low cost of this spectroscopy scheme are valuable for out-of-the-lab applications like lidar and environmental monitoring.

关键词： frequency comb gas spectroscopy integrated photonics radio-frequency modulation tunable laser spectroscopy

ORPA:a fast and efficient phylogenetic analysis method for constructing genome-wide alignments of organelle genomes

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Journal of Genetics and Genomics 2024年第3期51卷 352-358页

作者： Guiqi bi Xinxin Luan Jianbin Yan Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureKey Laboratory of Synthetic BiologyMinistry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenGuangdong 518124China Zhengzhou Research Base State Key Laboratory of Cotton BiologySchool of Agricultural SciencesZhengzhou UniversityZhengzhouHenan 450001China

Creating a multi-gene alignment matrix for phylogenetic analysis using organelle genomes involves aligning single-gene datasets manually,a process that can be time-consuming and prone to *** HomBlocks pipeline has been created to eliminate the inaccuracies arising from manual *** processing of a large number of sequences,however,remains a time-consuming *** conquer this challenge,we develop a speedy and efficient method called Organelle Genomes for Phylogenetic Analysis(ORPA).ORPA can quickly generate multiple sequence alignments for whole-genome comparisons by parsing the result files of NCbi BLAST,completing the task just in 1 *** increasing data volume,the efficiency of ORPA is even more pronounced,over 300 times faster than HomBlocks in aligning 60 high-plant chloroplast *** phylogenetic tree outputs from ORPA are equivalent to HomBlocks,indicating its outstanding *** to its speed and accuracy,ORPA can identify species-level evolutionary conflicts,providing valuable insights into evolutionary cognition.

关键词： Ultrafast alignment Organelle phylogenomics Phylogenomic conflict Efficient pipeline Multiple sequence alignment pipeline

bimetallic In_(2)O_(3)/bi_(2)O_(3) Catalysts Enable Highly Selective CO_(2) Electroreduction to Formate within Ultra-Broad Potential Windows

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Energy & Environmental Materials 2024年第1期7卷 257-264页

作者： Zhongxue Yang Hongzhi Wang Xinze bi Xiaojie Tan Yuezhu Zhao Wenhang Wang Yecheng Zou Huai ping Wang Hui Ning Mingbo Wu State Key Laboratory of Heavy Oil Processing College of Chemical EngineeringCollege of New EnergyChina University of Petroleum(East China)Qingdao 266580China Shandong Dongyue Future Hydrogen Energy Material Co. Ltd.Zibo 255000China

CO_(2)electrochemical reduction reaction(CO_(2)RR)to formate is a hopeful pathway for reducing CO_(2)and producing high-value chemicals,which needs highly selective catalysts with ultra-broad potential windows to meet the industrial ***,the nanorod-like bimetallic ln_(2)O_(3)/bi_(2)O_(3)catalysts were successfully synthesized by pyrolysis of bimetallic Inbi-MOF *** abundant oxygen vacancies generated from the lattice mismatch of bi_(2)O_(3)and ln_(2)O_(3)reduced the activation energy of CO_(2)to*CO_(2)·^(-)and improved the selectivity of*CO_(2)·^(-)to formate ***,the carbon skeleton derived from the pyrolysis of organic framework of Inbi-MOF provided a conductive network to accelerate the electrons *** catalyst exhibited an ultra-broad applied potential window of 1200 mV(from-0.4 to-1.6 V vs RHE),relativistic high Faradaic efficiency of formate(99.92%)and satisfactory stability after 30 *** in situ FT-IR experiment and DFT calculation verified that the abundant oxygen vacancies on the surface of catalysts can easily absorb CO_(2)molecules,and oxygen vacancy path is dominant *** work provides a convenient method to construct high-performance bimetallic catalysts for the industrial application of CO_(2)RR.

关键词： bimetallic catalyst CO_(2)electrochemical reduction reaction formate oxygen vacancy wide potential window