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植物基因组表达序列标签(EST)计划研究进展

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生物化学与生物物理进展 2001年第4期28卷 494-497页

作者：骆蒙贾继增中国农业科学院品种资源研究所农业部作物种质资源与生物技术重点实验室北京100081

植物表达序列标签 (EST)计划是随机挑选cDNA克隆 ,并对其 3′或 5′端进行大规模一次性测序 ,将得到的 15 0～ 5 0 0bp长度的DNA片段与数据库中的序列进行比较 ,获得对基因组结构、组织、表达等认识的基因组研究策略 .就近年来国际植物... 详细信息

植物表达序列标签 (EST)计划是随机挑选cDNA克隆 ,并对其 3′或 5′端进行大规模一次性测序 ,将得到的 15 0～ 5 0 0bp长度的DNA片段与数据库中的序列进行比较 ,获得对基因组结构、组织、表达等认识的基因组研究策略 .就近年来国际植物EST计划的实施情况、植物EST计划的研究范围、生物信息学在EST研究中的应用、EST数据库及查询。

关键词：植物基因组表达序列标签生物信息学

Analysis of the essential DNA region for OsEBP-89 promoter in response to methyl jasmonic acid

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science China(Life sciences) 2008年第3期51卷 280-285页

作者： LI Ang1,2, CHEN LiangLiang1,3, REN HaiYun3, WANG XueChen2, ZHANG HaiWen1,4 & HUANG Rong- Feng1,4 1 Biotechnology Research institute, chinese academy of agricultural sciences, beijing 100081, China 2 National Laboratory of Plant Physiology and Biochemistry, College of Biological sciences, China agricultural University, beijing 100094, China 3 College of Life sciences, beijing Normal University, beijing 100875, China 4 National Key Facility of crop Gene Resources and Genetic Improvement, beijing 100081, China Biotechnology Research institute Chinese Academy of Agricultural Sciences Beijing 100081 China National Laboratory of Plant Physiology Biochemistry College of Biological Sciences China Agricultural University Beijing 100094 China College of Life sciences Beijing Normal University Beijing 100875 China National Key Facility of crop Gene Resources Genetic Improvement Beijing 100081 China

In rice, the characterization of OsEBP-89 is inducible by various stress- or hormone-stimuli, including ethylene, abscisic acid (ABA), jasmonate acid (JA), drought and cold. Here, we report the investigation of essential DNA region within OsEBP-89 promoter for methyl jasmonic acid (MeJA) induction. PLACE analysis indicates that this promoter sequence contains multiple potential elements in response to various stimuli. First, we fused this promoter with GUS gene and analyzed its expression under MeJA treatment through Agrobacterium infiltration mediating transient expression in tobacco leaves. Our results revealed that this chimeric gene could be inducible by MeJA in tobacco leaves. To further de- termine the crucial sequences responsible for MeJA induction, we generated a series of deletion pro- moters which were fused with GUS reporter gene respectively. The results of transient expression of GUS gene driven by these mutant promoters show that the essential region for MeJA induction is po- sitioned in the region between -1200 and -800 in OsEBP-89 promoter containing a G-box (?1127), which is distinct from the essential region containing ERE (?562) for ACC induction. In all, our finding is helpful in understanding the molecular mechanism of OsEBP-89 expression under different stimuli.

关键词： OsEBP-89 essential DNA region methyl jasmonic acid transient assay promoter tobacco leaves

Land cover changes based on plant successions: Deforestation, rehabilitation and degeneration of forest in the upper Dadu River watershed

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science China Earth sciences 2005年第12期48卷 2214-2230页

作者： YAN Jianzhong1,2,3, ZHANG Yili1, BAI Wanqi1, LIU Yanhua4, BAO Weikai5, LIU Linshan1, 2 & ZHENG Du1 1. institute of Geographic sciences and Natural Resources Research, chinese academy of sciences, beijing 100101, China 2. Graduate School of the chinese academy of sciences, beijing 100039, China 3. College of Resources and Environment, Southwest agricultural University, Chongqing 400716, China 4. The Ministry of science and Technology, P. C. China, beijing 100862, China 5. Chengdu institute of Biology, chinese academy of sciences, Chengdu 610041, China 1. institute of Geographic sciences and Natural Resources Research Chinese Academy of Sciences 100101 Beijing China 2. Graduate School of the chinese academy of sciences 100039 Beijing China 3. College of Resources and Environment Southwest Agricultural University 400716 Chongqing China 4. The Ministry of science and Technology P. C. China 100862 Beijing China 5. Chengdu institute of Biology Chinese Academy of Sciences 610041 Chengdu China

To link regional land use/cover changes with environmental effects, land cover changes are required to reflect vegetation successions, whereas the land cover classification systems commonly used nowadays cannot serve this purpose. In this paper, a new land cover classification system is established in which land covers are classified by the vegetation succes-sions, taking Zamtang County, Barkam County and Jinchuan County in the upper Dadu River watershed as a study area. Using multi-temporal remote sensing images, the land cover data of 1967, 1986 and 2000 are obtained by means of integration of unsupervised classification and visual interpretation methods. The database facilitates the study of land use/cover changes, en-vironmental effects and ecological construction. Land cover changes reflect the main ecological processes in the upper Dadu River watershed. The landscape composed mainly of grasslands, wildwoods and alpine scrubs in 1967 was changed to that of grasslands, secondary forests, al-pine scrubs, fragmentary wildwoods, artificial forests, secondary scrubs in 2000, meanwhile, the landscape got more fragmentized. The total area of the forests decreased by 9.43%. Study results have shown the process of restoration of logged areas in forest centers. From 1967 to 2000, only 6.86 percents of logged areas were converted to shrubs, meadows or crop-lands, and the rest were converted into artificial forests or secondary forests. So the ecological shelter functions will be restored, stage by stage. Firewood collection, charcoal production and overgrazing are the three major triggers for the extensive degradation of alpine oak forests, Sa-bina tibetica forests and meadows. The arid valley grasslands expanded too. The degradation of vegetation in the southern slopes impairs ecological shelter functions and affects livelihood of local residents, so it is essential to find effective measures for ecological restoration and recon-struction. Field investigations have found

关键词： land use/cover classification, upper Dadu River watershed, vegetation successions, GIS, RS.

DISCOVERY AND DETERMINATION OF A DOMINANT MALE-STERILE GENE AND

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science in China,Ser.B 1982年第5期25卷 508-520页

作者：邓景扬高忠丽 institute of crop Breeding and Cultivation chinese academy of agricultural sciences institute of agricultural sciences of Taigu County Shanxi Province China

This paper deals with the determination of one male-sterile plant found in chinese wheatcultivar (T. aestuvum L.). It is the first time in the world that a spontaneous mutant is dis-eovered in wheat. This dominant male-sterile gene is assigned as Ta 1. This determinationfirmly confirms the existence of the genic male-sterility and provides once again the scien-tific evidence to solve the argument of this problem. On the other hand, a breeding programof using this gene, especially in recurrent selection, is herewith proposed.

关键词： DISCOVERY AND DETERMINATION OF A DOMINANT MALE-STERILE GENE AND

Relationships of Aegilops tauschii revealed by DNA fingerprints:The evidence for agriculture exchange between China and the West

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Progress in Natural science:Materials International 2008年第12期18卷 1525-1531页

作者： Huiting Wei~a Jun Li~a Zhengsong Peng~b Baorong Lu~c Zhijun Zhao~d Wuyun Yang~a a crop Research institute,Sichuan academy of agricultural sciences,Chengdu 610066,China b Life science College,China West Normal University,Nanchong 637002,China c The Ministry of Education Key Laboratory for Biodiversity science and Ecological Engineering,institute of Biodiversity science,Fudan University,Shanghai 200433,China d chinese academy of Social science,beijing 110710,China [a]crop Research institute Sichuan Academy of Agricultural Sciences Chengdu 610066 China [b]Life science College China West Normal University Nanchong 637002 China [c]The Ministry of Education Key Laboratory for Biodiversity science and Ecological Engineering Institute of Biodiversity Science Fudan University Shanghai 200433 China [d]chinese academy of Social science Beijing 110710 China

Genetic diversity and relationships of wild goat grass (Aegilops tauschii Cosson) from Iran and Xinjiang,west China,as well as its weedy type from the Yellow River region of Shaanxi and Henan provinces in China were analyzed by simple sequence repeat (SSR) fingerprints.A high level of genetic diversity in Aegilops tauschii accessions from Iran was observed,and the richness of genetic diversity was followed by accessions from Shaanxi,Henan,and *** weedy type of Aegilops tauschii showed a close genetic relationship with the wild type from different regions in *** results indicated that the weedy Aegilops tauschii found in the Yellow River region was most likely introduced from Iran—the diversity center of Aegilops *** weedy Aegilops tauschii populations found in the Yellow River region may be brought into the central part of China as a weed species together with common wheat (Triticum aestivum L.) from the West during various periods of time in *** finding has provided strong evidence for the introduction of common wheat from the West into China via the Silk Road,and also demonstrated the important role of the Silk Road in the exchange of agri- culture and other relevant technologies between China and the West.

关键词： The Silk Road Aegilops tauschii Common wheat SSR fingerprints Genetic diversity Affinity crop dispersion

Construction of a soybean linkage map using an F_2 hybrid population from a cultivated variety and a semi-wild soybean

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chinese science Bulletin 1997年第12期42卷 1033-1037页

作者： ZHANG Deshui, DONG Wei, HUI Dongwei, CHEN Shouyi and ZHUANG Bingchang1. Plant Biotechnology Laboratory, institute of Genetics, chinese academy of sciences, beijing 100101, China 2 Soybean institute, Jilin academy of agricultural sciences, Jiltn 136100, China Plant Biotechnolcgy Laboratory Institute of Genetics Chinese Academy of Sciences Beijing China Soybean institute Jilin Academy of Agricultural Sciences Jilin China

ALTHOUGH soybean is one of world’s leading sources of seed oil and seed protein, the develop-ment of its genetic map has lagged behind that of other crops such as rice and maize. The maincauses for this can be attributed to the lack of genetic diversity among soybean accessions, thepaucity of classical markers, the big size and complexity of the genome, and the small size anddifficulty in distinguishing of the chromosomes. The classical genetic map of

关键词： soybean F2 population molecular linkage map.

Numerous experiments for the impact of warming climate on the phenology and grain yield of winter wheat

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Journal of Environmental sciences 1990年第4期2卷 11-17页

作者： Zhao Siqiang and ZhangYu The academy of Metorological science,State Meteorlogical Administration,beijing 100081,China

Based on the environmental and crop data at Zhenjiang City, in Jiangsu Province, middle east of China, the growing process of winter wheat was simulated by CERES-Wheat model assuming the daily average temperature is 1℃ to 2℃ higher than at the present, which are mostly possible for the change of climate because of enrichment of greenhouse gases in the atmosphere. The simulation results show that warming climate can promote development rate and shorten phenological stages of wheat, and the grain yield will be higher than present. Its impact on the kernel weight and grains per square meter were different for the plantings of various sowing dates. The results of this study suggest that substantial changes in agricultural production and management practices are needed to respond to the climatic changes expected to take place in China.

关键词： greenhouse gases warming climate winter wheat climatic change phenology.

Regional differences and determinants of built-up area expansion in China

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science China Earth sciences 2007年第12期50卷 1835-1843页

作者： HUANG JiKun1,2,ZHU LiFen3,1 & DENG XiangZheng1,2 1 Center for chinese agricultural Policy,chinese academy of sciences,beijing 100101,China 2 institute of Geographic sciences and Natural Resources Research,chinese academy of sciences,beijing 100101,China 3 Research Center of the Economy of the Upper Reaches of the Yangtze River,Chongqing Technology and Business University,Chongqing 400067,China 1. Center for chinese agricultural Policy Chinese Academy of Sciences Beijing 100101 China 2. institute of Geographic sciences and Natural Resources Research Chinese Academy of Sciences Beijing 100101 China 3. Research Center of the Economy of the Upper Reaches of the Yangtze River Chongqing Technology and Business University Chongqing 400067 China

Based on remote sensing data on land use provided by the chinese academy of sciences and socio-economic data collected by the authors,this paper analyzes the trends and regional differences in built-up area(BUA) expansion in China from the late 1980s to 2000,and empirically estimates the major determinants of BUA expansion in different regions in 1996―*** 1989―2000,although China's overall BUA expansion accelerated,the trends differed significantly among *** expansion in the central and western regions accelerated significantly,but it slowed down considerably in the east-ern *** estimation results from our econometric analysis reveal that BUA expansion in the eastern region reached a period when economic growth had no further significant impact on per capita BUA,the land utilization in this region has become more intensive with further expansion of the *** the central and western regions,the BUA has expanded remarkably due to the relatively more flexible land development policies and the relatively cheap land ***,as the econ-omy continues to grow rapidly,policies relating to BUA expansion and cultivated land reductions may face more serious challenges in the central and western regions.

关键词： built-up area, land use, regional difference, determinants, China

Chromosomal Localization of the 18S-5.8S-26S rDNA Loci in Thinopyrum ponticum （Podp.） Liu ＆ Wang and Related Diploid Species by Fluorescence In Situ Hybridization

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Chromosomal Localization of the 18S-5.8S-26S rDNA Loci in Th...