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细菌人工染色体基因组文库构建关键技术研究

生物技术通报 2008年第4期24卷 66-69页

作者：刘长青吴宏梅包阿东陆涛峰刘帅张洪海唐学玺关伟军马月辉中国农业科学院北京畜牧兽医研究所曲阜师范大学研究生处曲阜273165

基因组文库是进行分子克隆和基因结构与功能研究的基础,完整覆盖的基因组文库的构建,使基因组任何DNA片段的筛选和获得成为可能。细菌人工染色体(Bacterial Artificial Chromosome,BAC)与其它载体系统相比具有转化效率高、嵌合体少、插... 详细信息

基因组文库是进行分子克隆和基因结构与功能研究的基础,完整覆盖的基因组文库的构建,使基因组任何DNA片段的筛选和获得成为可能。细菌人工染色体(Bacterial Artificial Chromosome,BAC)与其它载体系统相比具有转化效率高、嵌合体少、插入片段易回收,高覆盖率、稳定性强,并且具有易分离和操作等特性等优点而被广泛应用。作为物种保护策略的重要部分,构建我国濒危动植物品种基因组BAC文库,对于其遗传资源的保护和研究具有非常重要的作用。在查阅大量国内外相关资料的基础上,就BAC基因组文库构建过程中载体的制备、高分子量DNA的制备、大片段DNA的回收、连接与电击转化、基因组BAC文库质量鉴定等几个重点、难点问题进行了详细的论述和分析,对于构建高分子量插入片段、高覆盖率和稳定性的基因组文库提供理论基础与技术支撑。

关键词：细菌人工染色体基因组文库构建质粒纯化高分子量DNA

冬前深耘断根对小麦根系活性的垂直分布及旗叶衰老的影响

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山东农业大学学报（自然科学版） 2001年第4期32卷 503-507页

作者：王法宏王旭清曹宏鑫任德昌于振文余松烈山东省农业科学院作物研究所济南250100 山东农业大学泰安271018

1996～ 1998年 ,用P2 O5同位素示踪技术研究了冬前深耘断根对小麦群体根系活性的垂直分布及旗叶衰老的效应。结果表明 ,冬前深耘断根可显著提高生育后期的小麦群体根系活性 ,使群体根系活性的垂直分布下移 ,深层土壤的群体根系活性相对... 详细信息

1996～ 1998年 ,用P2 O5同位素示踪技术研究了冬前深耘断根对小麦群体根系活性的垂直分布及旗叶衰老的效应。结果表明 ,冬前深耘断根可显著提高生育后期的小麦群体根系活性 ,使群体根系活性的垂直分布下移 ,深层土壤的群体根系活性相对提高。

关键词：小麦深耘断根根系活性垂直分布旗叶衰老

Spread of *** Aerosols Carrying Antibiotic-Resistant Genes （Including MRSA） from Chicken Farms to Their Neighborhood Residents Environment

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Spread of *** Aerosols Carrying Antibiotic-Resistant Genes ...

Prediction and Control for the Quantity of Rural Industrial crops by Using Statistical Methods

2012年环境污染与大众健康学术会议

作者： Dunjiang Liu 1 Tongjie Chai 1, * Yumei Cai 1 Lingyu Sun 1 Haiyu Hao 1 Jian Wang 2 1 College of Animal science and Veterinary Medicine, Shandong agricultural University, Sino-German Cooperative Research Centre for Zoonoses of Animal Origin of Shandong Province, Tai’an, 271018, China State Environmental Protection Key Laboratory of Microorganism Application and Risk Control, Tsinghua University, 100084 beijing, China. 2 Bureau of Animal Husbandry and Veterinary Zouping County, Shandong Province, China.

There is a rather limited understanding concerning the antibiotic-resistance of the airborne S. aureus and the transmission of the antibiotic-resistant genes it carries Therefore, we isolated 149 S. aureus strains from the samples collected from the feces, the indoor air and the outdoor air of 6 chicken farms, and performed the research on them with 15 types of antibiotics and the REP-PCR trace identification. The 100% homologous strains were selected to conduct the research on the carrying and transmission status of the antibiotic-resistant genes. The results revealed that 5.37% strains（8/149） were resistant to methicillins （MRSA）, and 94% strains （140/149） were resistant to compound sulfamethoxazole, etc. In addition, these strains displayed a resistance to multiple antibiotics （4, 5 or 6 types） and there were also 3 strains resistant to 9 antibiotics. the strains with the same REP-PCR trace identification result carried the same type of antibiotic-resistant genes. The results showed that airborne transmission not only causes the spread of epidemic diseases but also exerts threats to the public health of a community.

关键词： chicken farming environment aerosols of Staphylococcus aureus including MRSA REP-PCR tracing antibiotic-resistance antibiotic-resistant genes significance to epidemiology and public health.

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Prediction and Control for the Quantity of Rural Industrial ...

The Analysis of Rural Human Capital Accumulation Effect on the Transfer of Labor Employment

2012 International Conference on Computer science and Mathematics,Physical Education and Management（ICCMPE 2012）

作者： Wen Xi Duan School of Applied Mathematics beijing Normal University Zhuhai Zhuahi China Mei Ling Liu Library beijing Normal University Zhuhai Zhuahi China

Planting industrial crops is the main approaches for famers’ obtaining household income. However, the farmers often blindly arrange cultivated amount without the correct guidance, they can’t adapt to the market demand. Therefore, they cannot get the greatest economic income. By using statistical methods, the crop information of the planting profit can be collected. Therefore, we can forecast and control the planting quantity of the following year. In the production of the industrial crops, the planting quantity is a decreasing function of the purchasing price and the required quantity is a increasing function of the price. When the planting quantity is equal to the required amount, the planting profit is increased. So we should make the conclusion that the planting quantity equals the required. Sometimes farmers store up the industrial crops in order to have the much money in the next year. Whether the approach is reasonable or not depends on the next year’s planting quantity.

关键词： Industrial crops purchasing price planting cost planting profits growing quantity prediction and control

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The Analysis of Rural Human Capital Accumulation Effect on t...

Spatial variability of irrigation factors and their relationships with “corridor-barrier” functions in the Longitudinal Range-Gorge Region

Lecture Notes in Information Technology—2012 International Conference on Education Technology and Management Engineering(ETME 2012)

作者： Chen Yi-an School of Labor Economics Capital University of Economics and Business beijing 100026

This study aimed to examine the effects of human capital accumulation on the rural labor *** on the human capital theory,the study constructed Multinomial Logit model to examine the influence of human capital accumulation in rural areas on the outside employment options,the final conclusion:the low levels of human capital accumulation is an important factor that restricting the rural migrant workers into a higher levels of industry employment in a *** the long run,accelerating the accumulation of human capital in rural areas,as well as enhancing the levels of human capital in rural areas will not only help farmers non-agricultural employment and rural incomes,but also favorable to the industrial structure upgrading and urban needs.

关键词： Rural Area Human Capital Accumulation Labour Transfer Effects Analysis

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science Bulletin 2007年第S2期 33-41页

作者： GU ShiXiang~(1,2) HE DaMing~(3+) CUI YuanLai~1 XIE XianHong~1 LI YuanHua~4 1 State Key Laboratory of Water Resources and Hydropower Engineering science,Wuhan University,Wuhan 430072,China 2 Yunnan institute for Investigation,Design and Research of Water Resources & Hydropower Engineering,Kunming 650021,China 3 Asian International Rivers Center,Yunnan University,Kunming 650091,China 4 Department of Irrigation,Drainage and Rural Water Supply,Ministry of Water Resources,P.R.China,beijing 100053,China

Based on the monthly meteorological data at 58 meteorological stations from 1971 to 2000 in the Lon- gitudinal Range-Gorge Region（LRGR）and the surrounding areas,six factors including yearly ET0, maximum monthly ET0（May）,minimum monthly ET0（December）,paddy irrigation quota and total ag- ricultural irrigation quota（yearly and main irrigation period from April to June）were selected to exam- ine their spatial variability using a geostatistical *** results indicated that the coefficients of Moran's I,describing the Intensity of spatial autocorrelation,were greater in longitudinal direction than in other directions;the spatial variabilities of the six parameters were mainly caused by structural factor accounting for 60.2%—87.9%;the largest variabilities of ET0（yearly,in May and December）ap- peared in the northwest-southeast and northeast-southwest *** to the summer monsoon atmospheric circumfluence from the Indian Ocean and the Pacific Ocean,and continental warm-dry current in winter,the minimum fractal dimension and the maximum spatial variability of ET0（yearly,in May and December）occurred in southwest-northeast and southeast-northwest *** highest variabilities of paddy and total agricultural irrigation requirements occurred in the south-north direction due to the vapor and energy diffusion caused by corridor function of the longitudinal *** minimum spatial autocorrelation was in east-west direction,which proves that the six parameters of agricultural irrigation requirement were influenced by the"corridor-barrier"function in the LRGR and its surrounding areas.

关键词： reference crop evapotranspiration water demand of paddy irrigation agriculture irrigation quota spatial variability scale "corridor-barrier"function the Longitudinal Range-Gorge Region(LRGR)

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我国鸡白痢沙门氏菌病诊断与防治研究概况

西南民族大学学报（自然科学版） 2003年第2期29卷 190-195页

作者：沙莎孙裕光王琴西南农业大学荣昌校区重庆荣昌412460 中国兽药监察所北京100080

鸡白痢沙门氏菌可感染各日龄、不同品种的鸡,其临床症状表现不尽相同,均严重影响鸡的生产性能.细菌学方法仍是不可或缺的诊断手段.随着血清学技术、PCR技术、快速检测试剂盒逐步应用于鸡白痢的检疫之后,对鸡白痢的防制已跃上了一个新台... 详细信息

鸡白痢沙门氏菌可感染各日龄、不同品种的鸡,其临床症状表现不尽相同,均严重影响鸡的生产性能.细菌学方法仍是不可或缺的诊断手段.随着血清学技术、PCR技术、快速检测试剂盒逐步应用于鸡白痢的检疫之后,对鸡白痢的防制已跃上了一个新台阶,鸡白痢沙门氏菌的毒力质粒和耐药性研究,为药物、疫苗防治打开了新思路.减毒菌苗、灭活菌苗、脂多糖(LPS)抗原、卵黄抗体、活菌生物制剂、抗生素、磺胺药、中草药、有机酸等措施在鸡白痢的防治中发挥着重要作用.

关键词：鸡白痢沙门氏菌病诊断防治措施疫苗研制

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作物秸秆发酵转化高效菌株筛选及其初步鉴定

山东农业大学学报（自然科学版） 2001年第4期32卷 438-442页

作者：万鲁长黄春燕于迎春郭惠东曹德强史之煌韩振荣程业武山东省农科院植保所济南250100 即墨市科委即墨266200 即墨市土肥站即墨266200 定陶县植保站定陶274100

通过对筛选出的 9个不同菌株与常用菌株或自然菌群在秸杆基料上进行分型发酵对比试验检测 ,进一步验证了筛选菌株对秸秆复合料的发酵转化效能 ,供试的 5类型 9株菌发酵效能水平分别比同转化型对照菌皆提高 13%以上。对 9个菌株进行了初... 详细信息

通过对筛选出的 9个不同菌株与常用菌株或自然菌群在秸杆基料上进行分型发酵对比试验检测 ,进一步验证了筛选菌株对秸秆复合料的发酵转化效能 ,供试的 5类型 9株菌发酵效能水平分别比同转化型对照菌皆提高 13%以上。对 9个菌株进行了初步鉴定。

关键词：秸杆发酵菌株转化型筛选生物饲料有机肥料

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苏州高产稻区氮肥的经济施用

土壤学报 1983年第4期 373-386页

作者：陈荣业孙秀廷李阿荣潘遵谱陈全武惠茂新中国科学院南京土壤研究所江苏省苏州地区农业科学研究所

苏州地区是我国有名的农业集约地区之一,一年二熟或三熟,每熟作物施用氮肥的次数多,数量大,估计每季水稻的氮肥施用量按纯氮计一般超过每亩20斤。所施入的氮肥,据15N标记的氮肥去向研究结果,损失达30—70%。常年如此大量的施用氮肥加上... 详细信息

苏州地区是我国有名的农业集约地区之一,一年二熟或三熟,每熟作物施用氮肥的次数多,数量大,估计每季水稻的氮肥施用量按纯氮计一般超过每亩20斤。所施入的氮肥,据15N标记的氮肥去向研究结果,损失达30—70%。常年如此大量的施用氮肥加上如此严重的损失,不能不影响到农业的增产增收,对该地区的农田生态系统也可能是个潜在威胁,值得重视并设法加以改进。

关键词：深施尿素氮施氮肥肥料氮素利用率分次施肥氮肥施用追肥粒肥经济