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Isolation and functional characterization of the C-terminus of rice phos-phatidylinositol 4-kinase in vitro

Cell Research 2003年第2期13卷 131-139页

作者： XIANG FENG KONG, ZHI HONG XU, HONG WEI XUE, National Laboratory of Plant Molecular Genetics, institute of Plant physiology and Ecology, Shanghai institutes for Biological Science (SIBS), The Chinese Academy of Sciences Partner Group of Max-Planck-institute of Molecular Plant physiology (MPI-MP) on "Plant Molecular physiology and Signal Transduction", 300 Fenglin Road, 200032 Shanghai, China 1National Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS)The Chinese Academy of Sciences Partner Group el Max-Planck-institute of Molecular Plant physiology (MPI-MP) on “Plant Molecular physiology and Signal Transduction” 300 Fenglin Road 200032 Shanghai China 1National Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS)The Chinese Academy of Sciences Partner Group el Max-Planck-institute of Molecular Plant physiology (MPI-MP) on “Plant Molecular physiology and Signal Transduction” 300 Fenglin Road 200032 Shanghai China 1National Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS)The Chinese Academy of Sciences Partner Group el Max-Planck-institute of Molecular Plant physiology (MPI-MP) on “Plant Molecular physiology and Signal Transduction” 300 Fenglin Road 200032 Shanghai China

A partial rice (Oryza sativa L.) cDNA clone. OsPMK1c, was isolated through screening of a cDNA library constructed from tillering materials. OsPI4Klc encoded a peptide of 608 amino acids with a calculated molecular mass of 68.4 kDa. The OsPI4Klc peptide shared high homology and possessed the highly conserved domains present in most isolated cloned PI4-kinases, i.e. a lipid kinase unique (LKU) domain and a catalytic (CAT) domain. A region with similarity to pleckstrin homology (PH) domain was present in OsPI4K1c as well. Further comparison with genomic sequences in databases revealed that OsPI4K1c is located at the 3'-end of a putative rice PI 4-kinase coding gene OsPI4K1, and its coding region corresponded to the C-terminal half of OsPI4Kl protein. Twelve exons (49-562 bp in size) and 11 introns (77-974 bp in size) were identified in OsPI4K1c. The recombinant protein expressed in Escherichia coli phosphorylates phosphatidylinositol at the D4 position of the inositol ring. OsPI4K1 transcript levels were

关键词： Oryza sativa phosphatidylinositol 4-kinase.

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DNA chip-based expression profile analysis indicates involvement of the phosphatidylinositol signaling pathway in multiple plant responses to hormone and abiotic treatments

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Cell Research 2004年第1期14卷 34-45页

作者： Wen Hui LIN, Rui YE, Hui MA, Zhi Hong XU, Hong Wei XUE National Laboratory of Plant Molecular Genetics, institute of Plant physiology and Ecology, Shanghai institutes for Biological Sciences (SIBS), Chinese Academy of Sciences and Partner Group of Max-Planck-institute of Molecular Plant physiology (MPI-MP) on "Plant Molecular physiology and Signal Transduction", 300 Fenglin Road, 200032 Shanghai, China Xue HW Chinese Acad Sci Shanghai Inst Biol Sci Inst Plant Physiol & Ecol Natl Lab Plant Mol Genet 300 Fenglin Rd Shanghai 200032 Peoples R China

The phosphatidylinositol (PI) metabolic pathway is considered critical in plant responses to many environmental factors, and previous studies have indicated the involvement of multiple PI-related gene families during cellular responses. Through a detailed analysis of the Arabidopsis thaliana genome, 82 polypeptides were identified as being involved in PI signaling. These could be grouped into different families including PI synthases (PIS), PI-phosphate kinases (PIPK), phospholipases (PL), inositol polyphosphate phosphatases (IPPase), inositol polyphosphate kinases (IPK), PI transfer proteins and putative inositol polyphosphate receptors. The presence of more than 10 isoforms of PIPK, PLC, PLD and IPPase suggested that these genes might be differentially expressed during plant cellular responses or growth and development. Accordingly, DNA chip technology was employed to study the expression patterns of various isoforms. In total, 79 mRNA clones were amplified and used for DNA chip generation. Expression

关键词： phosphatidylinositol signal transduction DNA chip expression profile.

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Change of proton motive force across thylakoid membrane in soybean leaf during state transitions

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Chinese Science Bulletin 2003年第7期48卷 652-657页

作者： SU Jihu & SHEN Yungang Shanghai institute of Plant physiology, Chinese Academy of Sciences, Shanghai 200032, China Chinese Academy of Sciences

Change of proton gradient across thylakoid membrane in soybean leaves was studied with millisecond delayed light emission (ms-DLE) during the course of state transitions which were indicated by the chlorophyll fluores-cence at room temperature and 77 K. When dark-adapted leaves were induced to stateⅠ with far-red light, Fm/Fo, F685/F735 and the intensity of fast phase of ms-DLE were af-fected slightly. However, during the induction to stateⅡ with red light, both Fm/Fo and F685/F735 decreased immedi-ately and the former were quicker than the latter. In this interval, the intensity of fast phase of ms-DLE increased to a maximum and then decreased to a lower value during the transition to stateⅡ. Nigericin, an uncoupler which elimi-nates the proton gradient across thylakoid membrane, inhib-ited the increase in the intensity of fast phase of ms-DLE during the transition to stateⅡ. Another uncoupler, valino-mycin, which eliminates the membrane potential, did not affect the changes of the intensity of fast phase. These results suggest that the prompt increase in the intensity of fast phase of ms-DLE at the beginning of transitions to stateⅡ is cor-related mainly with the proton gradient released from water oxidation in photosystemⅡ.

关键词：大豆叶状态转换类囊体膜质子运动力量毫秒滞后光发射光合体系

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Identification of ABA-responsive genes in rice shoots via cDNA macroar- ray

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Cell Research 2003年第1期13卷 59-68页

作者： FANG LIN, SHOU LING XU, WEI MIN NI, ZHAO QING CHU, ZHI HONG XU, HONG WEI XUENational Laboratory of Plant Molecular Genetics, institute of Plant physiology and Ecology, Shanghai institutes for Biological Science (SIBS), Chinese Academy of Sciences and Partner Group of Max-Planck-institute of Molecular Plant physiology (MPI-MP) on "Plant Molecular physiology and Signal Transduc-tion", 300 Fenglin Road, 200032 Shanghai, China 1National Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences and Partner Group of Max-Planck-institute of Molecular Plant physiology (MPI-MP) on Plant Molecular physiology and Signal Transduction 300 Fenglin Road 200032 Shanghai China 1National Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences and Partner Group of Max-Planck-institute of Molecular Plant physiology (MPI-MP) on Plant Molecular physiology and Signal Transduction 300 Fenglin Road 200032 Shanghai China 1National Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences and Partner Group of Max-Planck-institute of Molecular Plant physiology (MPI-MP) on Plant Molecular physiology and Signal Transduction 300 Fenglin Road 200032 Shanghai China 1National Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences and Partner Group of Max-Planck-institute of Molecular Plant physiology (MPI-MP) on Plant Molecular physiology and Signal Transduction 300 Fenglin Road 200032 Shanghai China 1National Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences and Partner Group of Max-Planck-institute of Molecular Plant physiology (MPI-MP) on Plant Molecular physiology and Signal Transduction 300 Fenglin Road 200032 Shanghai China 1National Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences and Partner Group of Max-Planck-institute of Molecular Plant physiology (MPI-MP) on Plant Molecular physiology and Signal Transduct

Phytohormone abscisic acid (ABA) was critical for many plant growth and developmental processes including seed maturation, germination and response to environmental factors. With the purpose to detect the possible ABA related signal transduction pathways, we tried to isolate ABA-regulated genes through cDNA macroarray technology using ABA-treated rice seedling as materials (under treatment for 2, 4, 8 and 12 h). Of 6144 cDNA clones tested, 37 differential clones showing induction or suppression for at least one time, were isolated. Of them 30 and 7 were up- or down-regulated respectively. Sequence analyses revealed that the putative encoded proteins were involved in different possible processes, including transcription, metabolism and resistance, photosynthesis, signal transduction, and seed maturation. 6 cDNA clones were found to encode proteins with unknown functions. Regulation by ABA of 7 selected clones relating to signal transduction or metabolism was confirmed by reverse transcription PCR. In add

关键词： Oryza saliva abscisic acid (ABA) cDNA macroarray responsive genes signal transduc- tion

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THE RADIATION PROTECTIVE EFFECT OF SINAPINE DISTRIBUTED IN CRUCIFEROUS PLANTS ON GERMINATING SEEDS OF BARLEY AND WHEAT

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Science in China,Ser.B 1986年第7期29卷 723-732页

作者：顾瑞琦 Shamghai institute of Plant physiology Academia Sinica

Many species of Cruciferae are very resistant to ionizing radiation. The inhibition of barley growth induced by X-irradiation was reduced by pre-irradiation treatments with seedling extracts of Brassica napus, B. chinensis or B. juncea. The extracts of seedling of B. napus, B. chinensis, B. juncea, B. juncea var. crispifolia, B. oleracea, Raphanus sativus and the extracts of the infloreseences and leaves of Rorippa montana had the similar effect on wheat. The results showed that the radiation resistance of these cruciferous plants may relate to some natural radiation protective substances in these plants. Sinapine is widely distributed in Cruciferae. The reactions of rape extract in qualitative analysis were identical to those of sinapine. Pre-irradiation treatments of wheat and barley seeds with the solution of sinapine thiocyanate crystals extracted from rape showed similar radiation protective effect. The results of ESR spectra of sinapine-thymine binary systems showed that sinapine protected thymine

关键词： Th THE RADIATION PROTECTIVE EFFECT OF SINAPINE DISTRIBUTED IN CRUCIFEROUS PLANTS ON GERMINATING SEEDS OF BARLEY AND WHEAT

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Effects of phytoestrogen genistein on myocardial ischemia/reperfusion injury and apoptosis in rabbits

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Acta Pharmacologica Sinica 2004年第3期25卷 52-58页

作者： En-sheng JI,Hua YUE,Yu-ming WU,Rui-rong HE1 Department of physiology,institute ofBasic Medicine,Hebei MedicalUniversity,Shijiazhuang 050017,China Department of physiology Institute of Basic Medicine Hebei Medical University

AIM: To study the effect of genistein (GST) on rabbit heart ischemia/reperfusion (I/R) injury. METHODS: Rabbit heart I/R injury was induced by occluding the left anterior descending coronary artery for 45 min and reperfusing for 180 min. GST (1.0 mg/kg) was intravenously injected 5 min before heart ischemia. Hemodynamic data, infarct size, and cardiomyocytic apoptosis were measured. The pathologic changes of I/R myocardium were observed. RESULTS: During the I/R, heart rate, mean arterial blood pressure, myocardial oxygen consumption, left ventricu- lar (LV) –dp/dtmax and +dp/dtmax were decreased progressively. The infarct size was occupied 60.23 %±3.97 % (% of area at risk) in vehicle +I/R group while GST reduced the infarct size to 39.62 %±4.30 % (P<0.01). DNA ladder patter in myocardium was revealed by agarose gel electrophoresis in vehicle +I/R group while was not found in GST+I/R group. Apoptotic cardiomyocytes were sparse within ischemic myocardium at risk in GST+I/R group as compared with that in vehicle +I/R group (TUNEL stain). Apoptosis rate in ischemic myocardium from vehicle +I/R and GST+I/R groups detected by flow cytometry were 15.33 %±1.31 % and 3.88 %±0.33 %, respectively. Fas and Bax protein expressions in ischemic myocardium of vehicle +I/R group were higher than that in GST+I/R group (P<0.01). Bcl-2/Bax ratio in vehicle +I/R group was lower than that in nonischemic myocar- dium (P<0.01), while in GST+I/R group, the Bcl-2/Bax ratio was higher than that in vehicle +I/R group (P<0.01). CONCLUSION: GST reduced infarct size and apoptosis of myocytes in I/R rabbit heart.

关键词： genistein reperfusion injury apoptosis rabbits

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MORN motifs in plant PIPKs are involved in the regulation of subcellular localization and phospholipid binding

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Cell Research 2006年第5期16卷 466-478页

作者： Hui Ma~(1,*) Ying Lou~1 Wen Hui Lin~1 Hong Wei Xue~1 ~1National Key Laboratory of Plant Molecular Genetics,institute of Plant physiology and Ecology,Shanghai institutes for Biological Science(SIBS),Chinese Academy of Sciences and Parmer Group of Max-Planck-institute of Molecular Plant physiology(MPI-MP)on"Plant Molecular physiology and Signal Transduction",300 Fenglin Road,200032 Shanghai,China National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences Partner Group of Max-Planck-institute of Molecular Plant physiology (MPI-MP) on “Plant Molecular physiology and Signal Transduction” Shanghai China Unit of Molecular Signal Transduction ERRB NICHD Bldg 49 Bethesda

Multiple repeats of membrane occupation and recognition nexus （MORN） motifs were detected in plant phosphati-dylinositl monophosphate kinase （PIPK）,a key enzyme in PI-signaling *** analysis indicates that allthe MORN motifs （with varied numbers at ranges of 7-9）,which shared high homologies to those of animal ones,werelocated at N-terminus and sequentially arranged,except those of OsPIPK1 and AtPIPK7,in which the last MORN motifwas separated others by an～100 amino-acid"island"region,revealing the presence of two kinds of MORN arrangementsin plant *** employing a yeast-based SMET （sequence of membrane-targeting） system,the MORN motifswere shown being able to target the fusion proteins to cell plasma membrane,which were further confirmed by expressionof fused MORN-GFP *** detailed analysis via deletion studies indicated the MORN motifs in OsPIPK1,together with the 104 amino-acid"island"region are involved in the regulation of differential subcellular localization,*** membrane or nucleus,of the fused *** Western blot analysis of the recombinant MORN polypep-tide,expressed in Escherichia coli,showed that MORN motifs could strongly bind to PA and relatively slightly to PI4Pand PI（4,5）*** results provide informative hints on mechanisms of subcellular localization,as well as regulationof substrate binding,of plant PIPKs.

关键词： membrane targeting PIPK MORN motif subcellular localization

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Selective protection of glycinebetaine on extrinsic polypeptides of PS Ⅱ particles

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Chinese Science Bulletin 1997年第20期42卷 1744-1748页

作者： HOU Caixia, XU Chunhe, TANG Zhangcheng and SHEN YunkangShanghai institute of Plant physiology, Chinese Academy of Sciences, Shanghai 200032, China Shanghai institute of Plant physiology Chinese Academy of Sciences Shanghai China

PHOTOSYSTEM Ⅱ（PS Ⅱ） particles capable of O2 evolution contain D1, D2 transmembrane pro-teins, and three extrinsic polypeptides which are exposed to the outer aqueous phase with ap-parent molecular masses of 33, 23, 18 kD. The three proteins can be partially or totally re-moved by treating the PS Ⅱ membranes with high concentrations of salt or other methods,

关键词： glycinebetaine extrinsic polypeptides trichloroacetate NaCI.

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Foliar modifications induced by inhibition of polar transport of auxin

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Cell Research 1999年第1期9卷 27-35页

作者： NI DI AN LING JIAN WANG ZHI HONG XU ZHEN Ao XIA(Shanghai institute of Plant physiology, Chinese Academy of Sciences, Shanghai 200032, China) 1Shanghai institute of Plant physiology Chinese Academy of Sciences Shanghai 200032 China 1Shanghai institute of Plant physiology Chinese Academy of Sciences Shanghai 200032 China 1Shanghai institute of Plant physiology Chinese Academy of Sciences Shanghai 200032 China 1Shanghai institute of Plant physiology Chinese Academy of Sciences Shanghai 200032 China

The effects of auxin polar transport inhibitors, 9-hydroxy-fluorene-9-carboxylic acid (HFCA); 2, 3, 5-triiodobenzoic acid (TIBA) and trans-cinnamic acid (CA)on leaf pattern formation were investigated with shoots formed from cultured leaf explants of tobacco and cultured pedicel explants of Orychophragmus violaceus, and the seedlings of tobacco and Brassica chinensis. Although the effective concentration varies with the inhibitors used, all of the inhibitors induced the formation of trumpet-shaped and/or fused leaves. The frequency of trumpet-shaped leaf formation was related to the concentration of inhibitors in the *** observation of tobacco seedlings showed that there was only one main vascular bundle and several minor vascular bundles in normal leaves of the control, but there were several vascular bundles of more or less the same size in the trumpet-shaped leaves of treated *** results indicated that auxin polar transport played an important role on bilateral symmetry of leaf growth.

关键词： Brassica Chinensis Orychophragmus violaceus Nicotiana tabacum auxin polar transport foliar modification

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The Accumulation of Free Proline and Its Roles in Water-Stressed Sorghum Seedlings

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植物生理与分子生物学 1989年第1期 105-110页

作者： TANG Zhang-Cheng Shanghai institute of Plant physiology, Academia Sinica, Shanghai 200032

The role of proline has recently been a sub-ject of intensive research. This stems from:(1) the marked accumulation of free prolinein plants undergoing water stress, salinity, lowtemperature, air pollutants, etc. and ... 详细信息

关键词： line Pro HC In The Accumulation of Free Proline and Its Roles in Water-Stressed Sorghum Seedlings

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