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Neuroprotection by hypoxic preconditioning in mice involves histaminemediated expression of VEGF

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Neuroprotection by hypoxic preconditioning in mice involves ...

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Yan-Ying FAN~1,Jian-Xiang ZHANG~1,Lu-Yi ZHANG~1,Ping HE~1,Hua-Wei ZHAO~1,Wei-Wei HU~1,Zhong CHEN~(1,2,*) 1 institute of Neuroscience,Department of Pharmacology,school of medicine,zhejiang university,hangzhou 310058, china 2 Department of Pharmacology,College of Pharmaceutical Sciences,zhejiang university,hangzhou 310058,china

Objective Cerebral ischemic tolerance is an adaptive process in which a brief subtoxic stress,such as hypoxic preconditioning,induces endogenous neuroprotection against a subsequent severe ischemia,but the mechanisms are poorly *** lines of evidence show that histamine mediates neuroprotective *** hypothesized that histamine is required for neuroproteciton induced by hypoxic *** Wild-type(WT) and histidine decarboxylase knock-out(HDC-KO) mice were preconditioned by 3 hours of hypoxia(8%O) and subjected to 30 min of transient middle cerebral artery occlusion(MCAO) 48 hours ***-fluoromethylhistidine(25 mg/kg,i.p.) was administered 3 hours before hypoxic preconditioning in WT *** deficit and infarct size were determined 24 hours *** histamine level in brain cortex of WT mice was measured by high-performance liquid chromatography immediately after 3 hours of *** endothelial growth factor(VEGF) mRNA(real-time PCR) and protein expression(western blot) in brain cortex of WT and HDC-KO mice were determined 0,3,8 and 24 hours after hypoxic ***,we treated cultured cortical neurons,astrocytes and bEDN.3 cells with histamine 30 min before hypoxia(0%O) and measured the induction of VEGF mRNA after *** Hypoxic preconditioning significantly improved neurological function and decreased infarct size in WT mice but not in HDC-KO or WT mice treated withα-*** decreased in the cortex of WT mice after 3 hours of hypoxia(65.5±10.2%vs. control,F<0.05),which indicates that hypoxia increased the release of histamine in mouse *** mRNA expression in WT mice showed a significantly greater increase after hypoxia than that in HDC-KO *** expression of VEGF 165 protein was induced by hypoxia in the cortex of WT mice but not of HDC-KO ***,histamine selectively enhanced the hypoxia-induced VEGF mRNA expression of astrocytes but not

关键词： histamine hypoxic preconditioning vascular endothelial growth factor

转禽冠状病毒免疫原基因马铃薯及其对小鼠的免疫原性

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生物化学与生物物理学报 2003年第11期35卷 1011-1015页

作者：吴建祥周继勇周雪平浙江大学动物预防医学研究所浙江大学生物技术研究所杭州310029浙江大学生物技术研究所杭州310029

为了探索禽冠状病毒免疫原基因在植物中表达的可行性 ,将传染性支气管炎病毒 (IBV) ZJ971毒株S1基因定向克隆到pBI1 2 1质粒的 35S启动子下游。用三亲交配法将重组质粒导入EHA1 0 5根癌农杆菌 ,建立了一种由农杆菌介导的马铃薯转化体系 ... 详细信息

为了探索禽冠状病毒免疫原基因在植物中表达的可行性 ,将传染性支气管炎病毒 (IBV) ZJ971毒株S1基因定向克隆到pBI1 2 1质粒的 35S启动子下游。用三亲交配法将重组质粒导入EHA1 0 5根癌农杆菌 ,建立了一种由农杆菌介导的马铃薯转化体系 ,其愈伤形成率达 1 0 0 % ,芽的分化率达 95 %。经PCR和Southern印迹检测证明 ,IBVS1基因已整合到植物基因组内 ,获 4 7株转基因植株。Northern印迹和ELISA分析表明 ,转基因植株中IBVS1基因能正常转录和翻译。转基因马铃薯的BALB/C小鼠免疫试验结果表明 ,0 .5g和 1 g剂量转基因马铃薯免疫小鼠 ,2 1天后的抗体ELISA效价分别达 1∶2 0～ 1∶4 0和 1∶80 1∶1 6 0 ,中和抗体效价分别达 1∶1 6 5～ 1∶5 6 2和 1∶330～ 1∶2 0 4 8,说明禽冠状病毒免疫原基因的转基因马铃薯表达产物具有免疫原性

关键词：禽冠状病毒免疫原基因转基因马铃薯免疫原性

二氧化硅对成纤维细胞和肺上皮细胞的DNA损伤作用

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中国职业医学 2002年第3期29卷 29-31页

作者：余晨曾昭玉吴巍巍浙江大学医学院劳动卫生与环境卫生研究所尘肺研究室浙江杭州310031

目的　研究小剂量二氧化硅 (SiO2 )粉尘对中国仓鼠肺成纤维细胞 (CHL)和貂肺上皮细胞 (CCL 6 4)的DNA损伤作用。方法　采用彗星试验 ,按SiO2 粉尘不同的作用浓度 (终浓度 0、7 5、15、30、6 0、12 0mg/L)和时间分组 ( 1h、2h)来观察DNA... 详细信息

目的　研究小剂量二氧化硅 (SiO2 )粉尘对中国仓鼠肺成纤维细胞 (CHL)和貂肺上皮细胞 (CCL 6 4)的DNA损伤作用。方法　采用彗星试验 ,按SiO2 粉尘不同的作用浓度 (终浓度 0、7 5、15、30、6 0、12 0mg/L)和时间分组 ( 1h、2h)来观察DNA损伤。以彗星尾长 (彗头末端到彗尾末端的长度 )作为DNA损伤程度的评价指标。结果　与对照组相比 ,SiO2明显增加了CHL及CCL 6 4细胞的DNA迁移距离 (P <0 0 1) ,在 7 5～ 12 0mg/L的剂量范围内 ,彗星尾长随作用剂量的增加而增加 ;一般 2h组比 1h组引起更严重的DNA损伤。结论　小剂量SiO2 粉尘确有遗传毒性 ,体外试验可致CHL细胞和CCL 6 4细胞DNA链断裂。由彗星试验的系列浓度SiO2

关键词：二氧化硅成纤维细胞肺上皮细胞 DNA损伤

Enhanced Antitumor Activity by a Selective Conditionally Replicating Adenovirus Combining with MDA-7/Interleukin-24 for B Lymphoblastic Leukemia via Induction of Autophagy

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Enhanced Antitumor Activity by a Selective Conditionally Rep...

第11次中国实验血液学会议

作者： Wenbin Qian~(1,2) Junqing Liu1 Yin Tong~1 Senxiang Yah~1 Min Yang~1 Xinyuan Liu~(2,3) 1.institute of Hematology,the First Affiliated Hospital,College of medicine,zhejiang university,Key lab of Combined Multi-Organ Transplantation,Ministry of Public Health,79# Qingchun Road,hangzhou 310003,china 2.Xinyuan institute of medicine and 8iotechnology,school of Life Sciences,zhejiang university of Technology and Sciences,hangzhou 310018,china 3.institute of Biochemistry and Cell Biology,Shanghai institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,china

Conditionally replicating adenoviru- ses(CRAds)represent a promising new platform for anticancer *** CRAds have been eval- uated extensively in ithelial twnors but little in hema- topoific malignancies because of the lack of the cox- sackie adenovirus receptor,CAR on their cell sur- *** this study, we showed at CAR was expressed on two types of lymphoblastic leukemia cell lines and primary leukemia cells,and that ZD55,a CRAd, exerted a potent antileukemia effect in vitro and in vi- ***,ZD55 expressmg melanoma differ- entiation-as soc iated gene-7/interleukin-24 (ZD55-IL-24)elicited significant enhanced anti leukemia activity comparing with ZD55,consistent with induction of *** data for the first time indicate that IL-24 exerts its antitumor effect on leukemia cells by not only inducing apoptosis but also stimulating autophagy,and suggest that oncolytic adenovirus,ZD55 and ZD55-IL-24 could poten- tially be used against CAR-expressing hematological malignancies such as B-lymphoblastic leukemia/ lymphoma and some myeloid leukemia.

关键词： replication-competent adenovirus leukemia Mda-7 interleukin-24 autophagy

Association of CASP9,CASP10 gene polymorphisms and tea drinking with colorectal cancer risk in the Han Chinese population

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Journal of zhejiang university-Science B(Biomedicine & Biotechnology) 2013年第1期14卷 47-57页

作者： He LIU Ming-wu ZHANG Yi-feng PAN Yun-xian YU Shan-chun ZHANG Xin-yuan MA Qi-long LI Kun CHEN Department of Epidemiology and Health Statistics School of Medicine Zhejiang University Hangzhou 310058 China institute of Cancer Research and Prevention of Jiashan County Jiashan 314100 China

The initiators caspase-9 （CASP9） and caspase-10 （CASPIO） are two key controllers of apoptosis and play important roles in carcinogenesis. This study aims to explore the association between CASPs gene polymorphisms and colorectal cancer （CRC） susceptibility in a population-based study. A two-stage designed population-based case-control study was carried out, including a testing set with 300 cases and 296 controls and a validation set with 206 cases and 845 controls. A total of eight tag selected single nucleotide polymorphisms （SNPs） in CASP9 and CASPIO were chosen based on HapMap and the National Center of Biotechnology Information （NCBI） datasets and genotyped by restriction fragment length polymorphism （RFLP） assay. Multivariate logistic regression models were applied to evaluate the association of SNPs with CRC risk. In the first stage, from eight tag SNPs, three polymorphisms rs4646077 （odds ratio （OR）AA＋AG： 0.654, 95% confidence interval （CI）： 0.406-1.055; P=0.082）, rs4233532 （ORcc： 1.667, 95% CI： 0.967-2.876; Oacm： 1.435, 95% CI： 0.998-2.063; P=0.077）, and rs2881930 （ORcc： 0.263, 95% CI： 0.095-0.728, P=0.036） showed possible association with CRC risk. However, none of the three SNPs, rs4646077 （ORAA＋AG： 1.233, 95% CI： 0.903-1.683）, rs4233532 （ORcc： 0.892, 95% CI： 0.640-1.243; ORcT： 1.134, 95% CI： 0.897-1.433）, and rs2881930 （ORcc： 1.096, 95% CI： 0.620-1.938; ORcT： 1.009, 95% CI： 0.801-1.271）, remained significant with CRC risk in the validation set, even after stratification for different tumor locations （colon or rectum）. In addition, never tea drinking was associated with a significantly increased risk of CRC in testing set together with validation set （OR： 1.755, 95% CI： 1.319-2.334）. Our results found that polymorphisms of CASP9 and CASPIO genes may not contribute to CRC risk in Chinese population and thereby the large-scale case-control studies might be in consideration. In addition, tea drinking was a protective factor for CRC.

关键词： CASP9 CASPIO Colorectal cancer Single nucleotide polymorphisms Susceptibility to cancer Tea drinking

Long-term synaptic plasticity induced by vibrissal stimuli in rat

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Long-term synaptic plasticity induced by vibrissal stimuli i...

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Ming-De HUANG,Man-Li SUN,Yong HAN,Shu-Min DUAN,Yan-Qin YU~* Department of Basic Medical Science,institute of Neuroscience,zhejiang university school of medicine,866~# Yu Hang Tang Road,hangzhou 310058,china

＜正＞Rats actively explore their environment by rhythmically sweeping their *** a consequence, neuronal activity in somatosensory pathways is modulated by the frequency of whisker *** potential role of rhythmic neuronal activity for the integration and consolidation of sensory signals,however,remains unexplored. Here,we show that a brief period of rhythmic vibrissal stimulation in anesthetized rats resulted in a frequencyspecific and long-lasting increase or decrease in the amplitude and slope of somatosensory-evoked potentials in the contralateral primary somatosensory（barrel） *** application of AP-5 revealed that this vibrissa-to-cortex long-term plasticity in adult rat in vivo is NMDA receptor-dependent and the long-lasting plasticity caused by rhythmic vibrissal stimulation is generated in barrel *** results also showed that the long-lasting enhancement of the vibrissa-to-cortex evoked response was side- but not *** vibrissal stimulation at 100 Hz applied to the vibrissa for 1 s in infant-trimmed[postnatal day（P） 1-7]rats could not cause an obvious long-lasting increase in the amplitudes of vibrissa-to-cortex evoked responses,but in rats whose whiskers clipped daily to within 2-3 mm of the skin surface for 7 d during P 29-35,the long-lasting increase in the amplitudes of vibrissa-to-cortex evoked responses could still be induced by 100 Hz vibrissal ***,our results suggest that natural,rhythmic patterns of whisker activity modify the cerebral processing of sensory information,providing a possible mechanism underlying sensory perception.

关键词： barrel cortex synaptic plasticity vibrissal stimulation

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人防御素的研究进展

生物工程学报 2003年第3期19卷 261-266页

作者：彭力徐志南方向明吴金民岑沛霖浙江大学生物工程研究所杭州310027 浙江大学医学院附属邵逸夫医院中心实验室杭州310016

人防御素是近年发现的人体内重要的内源性抗生素 ,已引起国内外生物和医学研究者的日益重视。这里综述了人防御素的组织分布、分子生物学特点、作用机理、医学及药用价值等方面的研究进展 ,对采用基因重组方法生产人防御素的方法进行了... 详细信息

人防御素是近年发现的人体内重要的内源性抗生素 ,已引起国内外生物和医学研究者的日益重视。这里综述了人防御素的组织分布、分子生物学特点、作用机理、医学及药用价值等方面的研究进展 ,对采用基因重组方法生产人防御素的方法进行了评述 ,并展望了防御素的应用前景和发展趋势。

关键词：抗菌肽组织分布分子生物学作用机理人防御素

Allelic frequency distributions of 21 non-combined DNA index system STR loci in a Russian ethnic minority group from Inner Mongolia,china

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Journal of zhejiang university-Science B(Biomedicine & Biotechnology) 2013年第6期14卷 533-540页

作者： Hong-dan WANG Chun-mei SHEN Wen-juan LIU Yu-dang ZHANG Guang YANG Jiang-wei YAN Hai-xia QIN Bo-feng ZHU school of medicine Xi'an Jiaotong University People's Hospital of Zhengzhou university Medical Genetic institute of Henan Province People's Hospital of Henan Province Blood Center of Shaanxi Province Vascular Biology Center Georgia Health Sciences UniversityAugustaGA 30912USA Beijing institute of Genomics Chinese Academy of Science Department of Internal medicine Morehouse School of MedicineAtlantaGA 30303USA

We studied the allelic frequency distributions and statistical forensic parameters of 21 new short tandem repeat(STR)loci and the amelogenin locus,which are not included in the combined DNA index system(CODIS),in a Russian ethnic minority group from the Inner Mongolia Autonomous Region,china.A total of 114 bloodstain samples from unrelated individuals were extracted and co-amplified with four fluorescence-labeled primers in a multiplex polymerase chain reaction(PCR)*** capillary electrophoresis,the PCR products of the 21 STR loci were separated and genotyped.A total of 161 alleles were observed in the Russian ethnic minority group,and corresponding allelic frequencies ranged from 0.0044 to *** 21 non-CODIS STR loci of the Russian ethnic minority group were characterized by high genetic diversity and therefore may be useful for elucidating the population's genetic background,for individual identification,and for paternity testing in forensic practice.

关键词： Short tandem repeat(STR) Russian population Genetic polymorphisms

Histamine attenuates autotomy by down-regulating Nav1.8 expression in DRG after peripheral nerve injury

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Histamine attenuates autotomy by down-regulating Nav1.8 expr...

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Jie YU~(1,2),Wen-Ting SHOU~1,Guo-Dong LOU~1,Wei-Wei HOU,Shi-Hong ZHANG~1,Zhong CHEN~(1,*) 1 Department of Pharmacology,institute of Neuroscience,school of medicine,zhejiang university,hangzhou,china 2 school of Pharmaceutical Sciences,Wenzhou Medical College,Wenzhou,china

＜正＞Objective To investigate the role of histamine in spontaneous neuropathic pain following peripheral nerve *** To prepare the model of spontaneous neuropathic pain（neuroma model）,histidine decarboxylase gene knock out（HDC-KO）mice and their wild-type littermates（n=14-16/group）underwent complete transection of the sciatic and saphenous nerves *** of mice was scored daily until postoperative day ***1.3, Nav1.7 and Nav1.8 protein levels in the sciatic nerve and L4/L5 DRGs from both wild-type and HDC-KO mice were measured by immunohistochemistry and west *** HDC-KO mice showed higher levels of autotomy than ***1.3,Nav1.7 and Nav1.8 protein levels significantly increase in the sciatic nerve after nerve transection both in HDC-KO mice and their wild-type littermates,and presence of endogenous histamine had no effect on this ***.3 expression was increased,while Navl.8 was decreased by axotomy in DRGs of both ***,a role of endogenous histamine was only detected in regulating Navl.8 expression in DRGs after *** These results indicate that endogenous histamine probably suppresses the development of spontaneous neuropathic pain by down-regulating Navl.8 expression in DRGs.

关键词： spontaneous neuropathic pain histamine Nav1.8 Nav1.7 Nav1.3