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High Throughput SNP Genotyping with Two Mini-sequencing Assays

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Acta Biochimica et Biophysica Sinica 2004年第6期36卷 379-384页

作者： Chunqing LUO1,2., Libin DENG1#, and Changqing ZENG1,2. The institute of Genetic and Developmental Biology, the chinese academy of sciences, {2. 100101, China 2.2. Genomics institute, the chinese academy of sciences, {2. 101300, China The institute of Genetic and Developmental Biology the Chinese Academy of SciencesBeijing 100101 China beijing Genomics institute the Chinese Academy of SciencesBeijing 101300 China

Two mini-sequencing methods, FP-TDI (template-directed dye-terminator incorporation with fluorescence-polarization) and MassArray (matrix assisted laser desorption ionization time of flight detection mass spectrometry), were optimized. A numeric standard was introduced to evaluate the SNP scoring quality of FP-TDI assay, thus made the optimization work easier. At the same time, using multi-PCR technology, 8-plex genotyping of MassArray assay was successfully carried out, some softwares were developed and the data process of MassArray was highly automated. Then these two methods were applied to high throughput SNP genotyping, the accuracy, efficiency and robustness were compared. The result shows FP-TDI is more sensitive to the concentration of SNPprimer and PCR product, as well as extension cycles, the SNPprimer length of FP-TDI should be 2.–30 bp long, whereas MassArray assay prefers to be as short as only 16 *** 6440 SNP sites of human chromosome 3 were genotyped in a sample of 90 individuals, 4792.sites by FP-TDI assay and 1648 sites by MassArray assay, the success rates of FP-TDI and MassArray were 67.7% and 93.6% respectively. The throughput of MassArray was higher than FP-TDI, and the cost of MassArray was lower, MassArray was more suitable for high throughput SNP genotyping.

关键词： SNP genotyping FP-TDI MassArray high throughput

“Tali Glaciation” on Massif Diancang

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Science China Earth sciences 2007年第11期50卷 1685-1692页

作者： YANG JianQiang1, CUI ZhiJiu2. YI ChaoLu1, SUN JiMin3 & YANG LiRong3 1 institute of Tibetan Plateau Research, chinese academy of sciences, {2. 100085, China 2.College of Environmental sciences, Peking University, {2. 100871, China 3 institute of geology and geophysics, chinese academy of sciences, beijing 1.002., China 1. institute of Tibetan Plateau Research Chinese Academy of Sciences Beijing 100085 China 2. College of Environmental sciences Peking University Beijing 100871 China 3. institute of geology and geophysics Chinese Academy of Sciences Beijing 100029 China

The term of ''Tali Glaciation'' is nominated from Massif Diancang in Yunnan Province. The confusing process of the term''s being put forward is confirmed through literature checking. Based on several times of field trip, the glacial landforms in this region are studied in detail, and the magnitude of the glaciation was determined. According to the numerical ages from TL, AIMS ^(14)C and OSL dating, the earliest glacial advance on Massif Diancang occurred at 30—40 ka BP, followed by the advances at the last stage of the last glaciation, the late glacial and neoglations in turn, until glaciers vanished at 1.2.1.5 ca. a BP in this region. It can be concluded that glaciers were limited above 3600 m a.s.l., and no glaciers existed on the lower part and adjacent mountains since the last glaciation.

关键词： Massif Diancang,Tali Glaciation,glacial landform,glaciation sequence,numerical dating

Genetic Mechanism of Mineral Inclusions in Zircons from the Khondalite Series, Southeastern Inner Mongolia

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Acta Geologica Sinica(English Edition) 2003年第1期77卷 28-35页

作者： LIU Fulai and SHEN Qihan institute of geology, {2. academy of Geological sciences, beijing 100037 chinese Acad Geol Sci Inst Geol Beijing 100037 Peoples R China

The early Precambrian khondalite series is widely distributed in theJining-Zhuozi-Fengzhen-Liangcheng area, southeastern Inner Mongolia. The khondalite series mainlyconsists of sillimanite garnet potash feldspar (or two-feldspar) gneiss and garnet biotiteplagioclase gneiss. These gneissic rocks have commonly experienced granulite-facies metamorphism. Inzircons separated from sillimanite garnet potash feldspar gneisses, many mineral inclusions,including Sil, Grt, Ky, Kfs, Qtz and Ap, have been identified by the Laser Raman ***, prograde metamorphic mineral inclusion assemblages such as Ky + Kfs + Qtz + Ap and Ky +Grt + Kfs + Qtz are preserved in the core of zircon, while peak granulite-facies metamorphicminerals including Sil + Grt + Kfs + Qtz and Sil + Gif + Kfs + Qtz + Ap are identified in the mantleand rim of the same zircon. However, in some zircons are only preserved the peak metamorphicminerals such as Sil + Grt + Kfs + Qtz and Sil + Grt + Kfs + Qtz + Ap from core to rim, and inothers are inherited the primary cores with minor mineral inclusions of Kfs + Qtz, with peakmetamorphic mineral inclusions around the inherited cores. These data indicate that the mineralassemblage evolution of sillimanite garnet potash feldspar gneisses in the study are did experiencea polymorphic transformation of kyanite to sillimanite. In garnet biotite plagioclase gneisses,secondary electron microscopic images reveal that most zircons display distinct zoning textures,which comprise cores and rims, each with distinctive inclusion assemblages. The inherited mineralinclusions, mainly consisting of Kfs + Pl + Qtz, Kfs + Qtz and Kfs + Qtz + Ap, are preserved in theprimary cores, while peak granulite-facies mineral asemblages, including Grt + Bt + Pl + Qtz + Ap,Grt + Bt + Pl + Qtz and Grt + Bt + Pl + Qtz + Rt, are identified on the rims. The occurrence of peakmetamorphic mineral inclusions in zircons indicates that these gneissic rocks, includingsillimanite garnet potas

关键词： mineral inclusion zircon Laser Raman secondary electron microscopy genetic mechanism khondalite series Inner Mongolia

MOLECULAR DYNAMICS SIMULATIONS OF FILLED AND EMPTY CAGE-LIKE WATER CLUSTERS IN LIQUID WATER AND THEIR SIGNIFICANCE TO GAS HYDRATE FORMATION MECHANISMS

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化工学报 2003年第Z1期54卷 62-66页

作者： GUO Guangjun,ZHANG Yigang and ZHAO Yajuan institute of geology and geophysics,chinese {2. of sciences beijing 1.002.,chinese institute of geology and geophysics Chinese Academy of Sciences Beijing 100029 China

Molecular dynamics simulations are performed to observe the evolutions of 512.and 512.2.cage-like water clusters filled with or without a methane molecule immersed in bulk liquid water at 2.0 K and 2.0 K. The lifetimes of these clusters are calculated according to their Lindemann index δ (t) using the criteria of δ≥0.07. For both the filled and empty clusters, we find the dynamics of bulk water determines the lifetimes of cage-like water clusters, and that the lifetime of 512.62.cage-like cluster is the same as that of 512.cage-like cluster. Although the methane molecule indeed makes the filled cage-like cluster more stable than the empty one, the empty cage-like cluster still has chance to be long-lived compared with the filled clusters. These observations support the labile cluster hypothesis on the formation mechanisms of gas hydrates.

关键词： like in time that were MOLECULAR DYNAMICS SIMULATIONS OF FILLED AND EMPTY CAGE-LIKE WATER CLUSTERS IN LIQUID WATER AND THEIR SIGNIFICANCE TO GAS HYDRATE FORMATION MECHANISMS of cage GAS

Characteristics of the Middle-Late Triassic sedimentary facies assemblages in the Songpan-Ruoergai area

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Progress in Natural Science:Materials International 2007年第3期17卷 305-313页

作者： YAN Zhen, YU Liangjun, LI Jiliang, BIAN Qiantao, WANG Zongqi and YANG Yongcheng ( institute of geology, {2. academy of Geological sciences, beijing 100037, China State Key Laboratory of Lithospheric Evolution, institute of geology and geophysics, chinese academy of sciences, beijing 1.002., China Shaanxi Insititute of geology Survey, Xianyang 712.00, China) [a]institute of geology Chinese Academy of Geological Sciences Beijing China [b]State Key Laboratory of Lithospheric Evolution Institute of Geology and Geophysics Chinese Academy of Sciences Beijing China [c]Shaanxi Insititute of geology Survey Xianyang China

The Middle-Late Triassic sedimentary rocks in the Songpan-Ruoergai area mainly consist of calcareous siltstone, muddy limestone, lithic arkose, feldspathic litharenite, mudstone, wormkalk, oolithic limestone, and conglomerate. Except for limestone bed increasing eastward longitudinally and vertically, the calcareous component of the sandstones increases obviously. Abundant benthic and plant fossils and their clasts occur within these rocks. The sedimentary structures predominately contain flaser, parallel, tabular, wavy, and herringbone cross beddings. These data coevally imply that the Middle-Late Triassic sediments deposited in the fluvial, lake and tide environments. Additionally, the rocks display graded, parallel, hummocky beddings, and sandy lamination, a feature characteristic of storm deposits.

关键词： Middle-Upper Triassic fluvial-lake environments tide environment tempestite Songpan-Ruoergai

Some key technics of drop tower experiment device of National Microgravity Laboratory (China) (NMLC)

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Science China(Technological sciences) 2005年第3期48卷 305-316页

作者： ZHANG Xiaoqian 1 , YUAN Longgen 1 , WU Wendong 1 , TIAN Lanqiao 2.& YAO Kangzhuang 2.1. institute of Engineering Thermophysics, chinese academy of sciences, {2. 100080, China 2. institute of Mechanics, chinese academy of sciences, {2. 100080, China 1. institute of Engineering Thermophysics Chinese Academy of Sciences 100080 Beijing China 2. institute of Mechanics Chinese Academy of Sciences 100080 Beijing China

Drop tower is an important ground based facility for microgravity science experiment. The technical performances of the drop tower NMLC are advanced compared with similar facilities in the US, Germany and Japan. The main components such as drop capsule, deceleration devices, release mechanism present its advantages and creativities.

关键词： drop tower facility,drop capsule,deceleration devices,release mechanism.

^(40)Ar-^(39)Ar ages of the Louzidian-Dachengzi ductile shear zone near Chifeng,Inner Mongolia and their tectonic significance

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chinese Science Bulletin 2002年第15期47卷 1292-1297页

作者： ZHANG Xiaohui, LI Tiesheng, PU Zhiping & WANG HuiResearch Center for Mineral Resources Exploration, institute of geology and geophysics, chinese academy of sciences, {2. 100101, China Guangzhou institute of Geochemistry, chinese academy of sciences, G Research Center for Mineral Resources Exploration Institute of Geology and Geophysics Chinese Academy of Sciences Beijing China Guangzhou institute of Geochemistry Chinese Academy of Sciences Guangzhou China

The Louzidian normal fault occurs as the eastern detachment fault of the Kalaqin metamorphic core complex. Field observations and microstructural analyses reveal that the Louzidian-Dachengzi ductile shear zone developed in its lower-plate was genetically related to sinistral strike-slips and extensional faulting. Two samples from this ductile shear zone yield 40Ar-39Ar plateau ages of 133 Ma (Bi) and 12. Ma (Kp), which are concordant with their isochron ages. The plateau age of 133 Ma (Bi) records the formation age of the ductile shear zone. The inconsistent relationship between the earlier strike-slip ductile shear zone and the later normal fault makes the Kalaqin Quasi-metamorphic core complex distinctive from Cordil-leran nietamorphic core complex. These ages provide important geochronological data for putting constraints on the formation age and genesis of such ductile shear zones.

关键词： 40Ar-39Ar age Sinistral strike-slip Louzidian-Dachengzi ductile shear zone Metamorphic core complex.

Algebraically explicit analytical solu-tions for the unsteady non-Newtonian swirling flow in an annular pipe

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Science China(Physics,Mechanics & Astronomy) 2006年第4期49卷 396-400页

作者： CAI Ruixian1 & GOU Chenhua1,2.1. institute of Engineering Thermophysics, chinese academy of sciences, {2. 100080, China 2. Graduate School, chinese academy of sciences, {2. 100080, China 1. institute of Engineering Thermophysics Chinese Academy of Sciences Beijing 100080 China 2. Graduate School Chinese Academy of Sciences Beijing 100080 China

This paper presents two algebraically explicit analytical solutions for the incompressible unsteady rotational flow of Oldroyd-B type in an annular pipe. The first solution is derived with the common method of separation of variables. The second one is deduced with the method of separation of variables with addition developed in recent years. The first analytical solution is of clear physical meaning and both of them are fairly simple and valuable for the newly developing computational fluid dynamics. They can be used as the benchmark solutions to verify the applicability of the existing numerical computational methods and to inspire new differencing schemes, grid generation ways, etc. Moreover, a steady solution for the generalized second grade rheologic fluid flow is also presented. The correctness of these solutions can be easily proven by substituting them into the original governing equation.

关键词： analytical solution, Oldroyd-B type fluid, swirling flow, non-Newtonian.

Mineralization episode of porphyry copper deposits in the Jinshajiang-Red River mineralization belt: Re-Os dating

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Science China Earth sciences 2005年第2期48卷 192-198页

作者： WANG Denghong, QU Wenjun, LI Zhiwei, YIN Hanlong4 & CHEN Yuchuan institute of Mineral Resources, chinese {2. of Geological sciences, beijing 100037, China National Center of Rock and Mineral Analysis, chinese {2. of Geological sciences, beijing 100037, China Resource Assessment Center of Yunnan, Kunming 650011, China institute of geology and geophysics, chinese academy of sciences, beijing 100101, China chinese {2. of Geological sciences, beijing 100037, China 1. institute of Mineral Resources Chinese Academy of Geological Sciences 100037 Beijing China 2. National Center of Rock and Mineral Analysis Chinese Academy of Geological Sciences 100037 Beijing China 3. Resource Assessment Center of Yunnan 650011 Kunming China 4. institute of geology and geophysics Chinese Academy of Sciences 100101 Beijing China 5. chinese academy of Geological {2. 100037 Beijing China

Re-Os isotopic dating for the molybdenites from the porphyry copper deposits of the Jinshajiang-Red River mineralization belt in Yunnan Province yields isochron ages of 33.9±1.1 Ma for the Machangqing deposit and 34.4±0.5 Ma for the Tongchang deposit. This result shows that both the Machangqing and the Tongchang porphyry Cu-Mo deposits from two different ore-fields formed simultaneously. This new data and the published Re-Os model ages of molybdenite (35.4 Ma, 35.9 Ma, 36.2.Ma) of the Yulong porphyry copper deposit in Tibet, which is located in the same Jinshajiang-Red River mineralization belt as the Machangqing deposit and the Tongchang deposit, suggest that these three Cenozoic porphyry copper deposits in the Jinshajiang-Red River mineralization belt were contemporary for their mineralization episode. That is to say, even their present locality is far away and nearly iso-distantly distributed, these three porphyry Cu(Mo) deposits belong to the same mineralization episode at the end of Eocene.

关键词： Re-Os isotopic dating, porphyry Cu deposits, Cenozoic, mineralization episode.