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Identification of differentially expressed genes in photo-period sensitive genic male sterile rice by randomly amplified cDNAs using RAPD primers

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Chinese Science Bulletin 1999年第4期44卷 348-351页

作者： JIANG Shuye, CHEN Qifeng and FANG Xuanjun Biotechnology Research Center, Chinese Academy of agricultural Science, Beijing 100081, china institute of genetics and crop breeding, fujian agricultural university, fuzhou 350002, china corresponding author Biotechnology Research Center Chinese Academy of Agricultural Science Beijing China institute of genetics and crop breeding Fujian Agricultural University Fuzhou China

The fertile and sterile young panicle representational populations were constructed by bulked sampling method using young panicles in the photo-period sensitive stage of fertility *** populations were analyzed using cDNA-RAPD. The results showed that: ( ⅰ ) bulked sampling method can be employed to analyze differentially expressed genes using cDNA-RAPD, taking an advantage in avoiding false positive caused by conventional sampling method. (ⅱ) Among 150 random primers used, 83 primers amplified the same banding patterns, and 34 primers amplified the same banding pattern but different staining of intensity on gel. ( ⅲ ) 33 primers amplified differential cDNA bands between fertile and sterile cDNA populations, and the ratio of polymorphism was 22%. It is concluded that there may exist a lot of genes relating to sterility, which makes the differentially expressed cDNA fragments complicated.

关键词： photo-period sensitive genie male sterile genes cDNA-RAPD differential display analysis.

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breeding Technology of eui-hybrids of Rice

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agricultural Sciences in china 2002年第4期1卷 359-363页

作者： YANG Ren-cui, ZHANG Shu-biao, HUANG Rong-hua, YANG Shu-Ian and ZHANG Qing-qi(institute of genetics and crop breeding , fujian agricultural and Forestry university, fuzhou 350002 , P. R . china ) institute of genetics and crop breeding Fujian Agricultural and Forestry University Fuzhou 350002 P.R. China

Induced mutants of elongated uppermost internode (eui) were directly obtained after irradiation on maintainer (B) and restorer (R) lines of hybrid rice. CMS lines(A) with eui (referred to as eA), restorer lines with eui (referred to as eR) as well as hybrid rice with eui gene (referred to as e-hybrids)were developed. The results showed that the eui mutation frequency averaged 0.10‰ at a range of 0.02‰- 0.47‰ varying with applied dosage and mutated materials. Genetic diversities of eui stock, and a new eui gene, temporarily designated as eui2(t), was identified. eA lines have a significantly elongated uppermost internode causing better panicle exsertion and possessing higher outcrossing potential, e-hybrids increased plant height by 3 -10 cm and headed 2-3 days earlier, as well as showed greater grain weight resulting in higher yield potential than that of corresponding original hybrids. The technology systems without GA3, a few of using GA3 in hybrid rice seed production, and the establishment of e-hybrid rice breeding system were also discussed in this paper.

关键词： Rice Radiation mutation eut-gene eui -CMS lines eui -restorer lines eui -hybrid rice

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不同eui基因对杂交稻干物质积累的影响

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上海交通大学学报（农业科学版） 2003年第2期21卷 139-143页

作者：章清杞陈邦东梁康迳李毓杨仁崔福建农林大学作物遗传育种研究所福州350002 福建省建瓯市农技中心建瓯353100

分别取带有eui1(t)、eui2(t)基因的长穗颈不育系及其对照2对,以及带有eui1(t)基因的恢复系及其对照2对,以不完全半双列杂交方式组配成遗传背景一致、但eui基因型不同的一套4组共16个F1材料,采用随机区组设计,对其干物质积累进行研究。... 详细信息

分别取带有eui1(t)、eui2(t)基因的长穗颈不育系及其对照2对,以及带有eui1(t)基因的恢复系及其对照2对,以不完全半双列杂交方式组配成遗传背景一致、但eui基因型不同的一套4组共16个F1材料,采用随机区组设计,对其干物质积累进行研究。结果表明:含有eui基因使杂交稻的茎、叶干物质生产能力增强,且以eui1(t)基因效果最大,eui2(t)基因效果不显著,而同时含有eui1、2基因的效果则相当或略小于eui1(t)基因;eui基因对穗干物质重影响较小;e-杂交稻的群体生长率也比不含eui基因的对照高,以eui1(t)基因的作用最为显著,作用强度依次为:eui1(t)>eui1(t)+eui2(t)>eui2(t),eui基因对杂交稻群体生长率增加作用主要是在生长前期和中期。eui基因对物质输出和转化影响较小。

关键词： eui基因杂交稻干物质积累遗传杂交方式

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