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新致病基因在颅缝早闭Crouzon综合征中的初步机制研究

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组织工程与重建外科杂志 2012年第5期8卷 256-263页

作者：杨娴娴 Jodie t.Hat.ield Susan J Hinze 穆雄铮 Pet.r J Anderson Barry C Powell 上海交通大学医学院附属第九人民医院整复外科上海市200011 澳大利亚颅颌面外科中心阿德莱德妇女儿童健康研究中心复旦大学医学院附属华山医院整形外科上海市200040

目的研究Rbp4(Ret.nol binding prot.in 4)、Gpc3(Glypican family of growt. fact.r binding prot.in 3)、C1qt.f3(Collagenous repeat.cont.ining sequence of 26 KDa prot.in)等新致病基因在颅缝早闭症中的发病机制,为疾病非手术治疗奠定理论基础。方法以Crouzon综合征Fgfr2cC342Y/+小鼠为实验模型,应用MicroCt.组织学染色,研究小鼠颅缝闭合模式;以Fgfr2cC342Y/+模型,Rt.qPCR研究Rbp4、Gpc3、C1qt.f3等新致病基因的表达差异,初步探讨其在颅缝闭合过程中的调控作用;以体外培养的Fgfr2cC342Y/+小鼠颅缝细胞为模型,研究基因突变动物细胞增殖与代谢改变。结果获得Fgfr2cC342Y/+小鼠后额缝、冠状缝、人字缝、矢状缝等颅缝闭合模式,随颅骨发育、颅缝闭合,OC(Ost.ocalcin)、ALP(Alkalinephosphat.se)表达增加,目的基因Rbp4、Gpc3、C1qt.f3表达下降,Msx2(Muscle segment.homeobox gene 2)表达增加,杂合子与野生型小鼠之间均存在显著统计学差异,与前期人颅缝组织Microarray研究结果一致。Gpc1(Glypican family ofgrowt. fact.r binding prot.in 1)、FliI(Flight.ess I)在颅缝闭合中的表达较恒定,野生型与杂合子之间未见明显统计学差异。体外培养Fgfr2cC342Y/+小鼠颅缝细胞,Cellt.t.r96 Mt.和Quant.it.Picogreen dsDNA细胞增殖与代谢分析结果显示,Fgfr2功能获得性突变可促进冠状缝细胞的增殖,从而导致成骨增加,颅缝早闭。结论 Fgfr2cC342Y/+模型新致病基因表达趋势与前期人颅缝组织Microarray研究结果一致,Rbp4、Gpc3、C1qt.f3可能在颅缝早闭中具重要调控作用。

关键词：颅缝早闭症 Fgfr2cC342Y/+小鼠 Crouzon综合征 Rt.qPCR 微量Ct Rbp4 Gpc3 Clqt.f3

超高分辨率和标准分辨率OCt.显示黄斑裂孔病理及修复中的比较研究

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世界核心医学期刊文摘（眼科学分册） 2005年第3期1卷 55-56页

作者： Ko t.H. Fujimot. J.G. Duker J.S. 黎黎 Dept. Elec. Eng. Comp. Sci. Res. L. Massachusetts Inst. of Technology Cambridge MA United States Dr

Purpose t. compare ult.ahigh resolut.on opt.cal coherence t.mography (UHR OC t. t.chnology t. a st.ndard resolut.on OCt.inst.ument.for t.e imaging of macula r hole pat.ology and repair; t. ident.fy sit.at.ons where UHR OCt.provides addi t.onal informat.on on disease morphology, pat.ogenesis, and management. and t. u se UHR OCt.as a baseline for improving t.e int.rpret.t.on of t.e st.ndard reso lut.on images. Design Observat.onal and int.rvent.onal case series. Part.cipant. t.ent. nine eyes of 24 pat.ent. clinically diagnosed wit. macular hole in at.l east.one eye. Met.ods A UHR OCt.syst.m has been developed and employed in a t.r t.ary care opht.almology clinic. Using a femt.second laser as t.e lowcoherence light.source, t.is new UHR OCt.syst.m can achieve an unprecedent.d 3 μm axial resolut.on for ret.nal OCt.imaging. Comparat.ve imaging was performed wit. UHR OCt.and st.ndard 10 μm resolut.on OCt.in 29 eyes of 24 pat.ent. wit. various st.ges of macular holes. imaging was also performed on a subset.of t.e populat. on before and aft.r macular hole surgery. Main out.ome measures Ult.ahigh and s t.ndard reso lut.on cross sect.onal OCt.images of macular hole pat.ologies. R esult. Bot. UHR OCt.and st.ndard resolut.on OCt.exhibit.d comparable performan ce in different.at.ng various st.ges of macular holes. t.e UHR OCt.provided imp roved imaging of finer int.aret.nal st.uct.res, such as t.e ext.rnal limit.ng me mbrane and phot.recept.r inner segment.(IS) and out.r segment.(OS), and ident.fi cat.on of t.e anat.my of successful surgical repair. t.e improved resolut.on of UHR OCt.enabled imaging of previously unident.fied changes in phot.recept.r mor phology associat.d wit. macular hole pat.ology and post.perat.ve repair. Visuali zat.on of t.e junct.on bet.een t.e phot.recept.r IS and OS was found t. be an im port.nt.indicat.r of phot.recept.r int.grit. for bot. st.ndard resolut.on and U HR OCt.images. Conclusions Ult.ahigh resolut.on opt.cal coherence t.mography i mproves t.

关键词：黄斑裂孔 OCt 标准分辨率光感受器细胞超高分辨率外节外界膜干预性相干性诊疗机构

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长期胃肠疾病患儿局部免疫反应增强

世界核心医学期刊文摘（儿科学分册） 2005年第5期1卷 9-10页

作者： Kokkonen J. Holm K. Kart.unen t.J. Mkim . 李开 Depart.ent.of Paediat.ics Oulu University HospitalPO Box 23FIN -90029 OysOuluFinland

Aim :Recent.y,we report.d t.pical endoscopic findings and an increment.in γδ+ t.ells in t.e foregut.among children wit. food-sensit.ve ent.ropat.y ot.er t.an coeliac *** find out.he ext.nd t. which t.e upregulat.on of t.e local immune response might.explain gast.oint.st.nal (GI)complaint. of t.e foregut.we sought.t. exam ine by t.e increm ent.in γδ+ t.cells a 1-y consecut.ve series of children referred for recurrent.G I com plaint. t. a t.r- t.ary-levelhospit.l.M et.ods:A 1-y cohort.of 102chil- dren scheduled for gast.oduodenoscopy were exam ined for m ucosalhist.logy and t.e densit.es ofCD3+,αβ+ and γδ+ t.cell subset. from m id-duodenal specim *** final diagnost.c cat.gories were used in analysing t.e dat.. Result.:Fift.en subject. showed villous at.ophy and a high γδ+ t.cell densit.;t.e finding being com pat.ble wit. coeliac disease (CD ).At.he ot.erext.em e,20subject. in whom diagnost.c G I diseases were ruled out.showed low densit.es and served as *** subject. report.ng GI sympt.m s aft.r an open food challenge wit. m ilk and /or cereals (n = 18)as well as children rem it.ingwit. am ilk-or cereal-elim inat.ng diet.but.not.responding t. a challenge (n = 23)also expressed significant.y higher densit.es ofγδ+ t.cells t.an t.e *** all,45of102 children could be considered t. have an elevat.d γδ+ t.cell densit. as an indicat.on of locally act.vat.d im m une re- sponse. Lack of villous archit.ct.re and lym phonodular hyperplasia of t.e duodenal bulb as an endoscopie finding and at.pic derm at.t.s but.not.t.e presence of D Q2alleles showed a close associat.on wit. t.ese increased densit.es. Conclusion:Considering t.at.n elevat.d incidence ofj6+ t.cells is an indicat.on of m ucosal response against.lum inal ant.gens,up t. halft.e children wit. prolonged G I sym p- t.m s have im m une m ediat.d disorder;CD and food allergy being t.e m ost.bvious clinicalent.t.es.

关键词：疾病患儿免疫反应细胞密度组织学检查食物过敏前肠细胞亚群免疫应答反复发作者免疫性疾病

EX-PRESS MINIAt.RE GLAUCOMA im- PLANt. imPLANt.t.ON UNDER A SCLE- RAL FLAP

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国际眼科杂志 2003年第1期3卷 104-104页

作者： E.Dahan t.Carmichael Oxford Eye Cen-t.e University of Witwatersrand South Africa

t.e Ex-PRESS (Opt.nol Lt.) is a miniat.re st.inless st.el glaucoma implant.wit. a 50-micron int.rnal lumen. t.o single-cent.r case series were performed t. evaluat. t.e safet. and efficacy of implant.t.on of t.e Ex-PR... 详细信息

关键词：青光眼微型Ex-PRESS埋植物巩膜瓣不埋植 IOP 随访

COMPARISON OF NON PENEt.At.NG FILt.At.ON SURGERY FOR OPEN ANGLE GLAUCOMA WIt. NON ABSORBABLE im-PLANt.(t.FLUX) VERSUS t.ABECULEC-t.MY: A MULt.CENt.IC St.DY

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国际眼科杂志 2003年第1期3卷 55-55页

作者： M.Dal Vecchio B.Brogliat.i t.Rolle F.M.Grignolo L.Bauchiero G.Vadala G.Caramello M.Bruni F.Molfino E.Gremmo Eye Clinic Universit. of t.rin Eye Clinic University of TurinEye Clinic University of TurinEye Clinic University of TurinEye Departmentt of Civil Hos-pitalEye Departmentt of Civil Hos-pitalEve Department of Santa Croce HospitalEve Department of Santa Croce HospitalEve Department of Santa Croce HospitalOcular Traumathology Department of Ophthalmic Hospital Italy

Purpose: t. compare t.e efficacy and safet. of deep sclerect.my wit. no absorbable implant.(t.flux) vs t.abeculect.my in POAG eyes. Met.ods: 23 eyes of 19 pat.ent. wit. medically uncont.olled open angle glaucoma underwent.t. deep sclerect.my wit. non absorbable implant.(t.flux). t.e post.perat.ve IOP values and t.e frequency of complicat.ons were mat.hed wit. t.ose of a group of 34 eyes of 34 POAG pat.ent. operat.d wit. t.abeculect.my. t.e examinat.ons were performed before surgery and post.

关键词：开角型青光眼非穿透性滤过术非可吸收埋植物小梁切除术比较研究

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浅析控制砖混结构开裂的问题

天津城市建设学院学报 1999年第2期5卷 26-28页

作者：贾绍平张裕媛天津城市建设学院土木工程系天津城市建设学院建筑设计研究院

目前砖混结构开裂最多的位置是首层窗台部位．按照锅底形沉降的规律，指出不宜在设计中将外墙下基础的地基承载能力设计值取得相对保守，与此相反，应该相对紧扣其地基承载能力来设计，另外再采取措施来控制窗台下墙壁体裂缝的出现，... 详细信息

目前砖混结构开裂最多的位置是首层窗台部位．按照锅底形沉降的规律，指出不宜在设计中将外墙下基础的地基承载能力设计值取得相对保守，与此相反，应该相对紧扣其地基承载能力来设计，另外再采取措施来控制窗台下墙壁体裂缝的出现，这样才是合理的设计．

关键词：砖混结构沉降承载能力窗台裂缝

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化学教学中素质教育的探讨

绥化师专学报 2002年第2期22卷 109-110页

作者：王庆商丘师范学院河南商丘476000

研究和探索实施素质教育的途径和方法,是全面推进素质教育,培养21世纪高素质人才的重要课题本文就高校大学生在上化学课时如何进行素质教育进行了系统的论述。

关键词：化学教学素质教育人才培养社会实践大学教育教学观念教学方法

超快速磁共振成像方案在诊断新发卒中患者中的应用

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世界核心医学期刊文摘（神经病学分册） 2005年第10期1卷 31-31页

作者： U-King-im J.M. t.ivedi R.A. Graves M.J. J.H. Gillard 王孝文 Depart.ent.of Radiology Addenbrooke’s Hospital University of Cambridge Cambridge CB2 2QQ United Kingdom Dr.

Object.ve: t. evaluat. t.e t.chnical feasibilit. of an int.grat.d ult.afast.h ead magnet.c resonance (MR) prot.col using a sensit.vit. encoding (SENSE) t.chni que for depict.ng parenchymal ischaemia and vascular compromise in pat.ent. wit. suspect.d recent.st.oke. Met.ods: 23 pat.ent. were evaluat.d wit. t.e ult.afast.MR prot.col using t., t., fluid at.enuat.d inversion recovery (FLAIR), 3D t.0}e of flight.magnet.c resonance angiography (MRA), and diffusion weight.d imaging ( DWI)- sequences. t.ese were compared wit. rout.ne convent.onal MR sequences. Re sult.: One pat.ent.could not.t.lerat. convent.onal imaging, alt.ough imaging usi ng t.e t.ree minut. head SENSE prot.col was diagnost.c. Bot. convent.onal and ul t.afast.prot.cols were of similar diagnost.c yield in t.e remaining pat.ent.. t. ere were no significant.differences in clinical diagnost.c qualit. for t.e t., t.2, FLAIR, and DWI sequences. One MRA examinat.on was of bet.er qualit. when SENS E was used, owing t. reduced mot.on art.fact. and short.r imaging t.0}e. Conclusi ons: It.is possible t. undert.ke a comprehensive MR examinat.on in st.oke pat.en t. in approximat.ly t.ree t. five minut.s. Ult.afast.imaging may become a useful t.iage t.ol before t.rombolyt.c t.erapy. It.may be of part.cular benefit.in pat.ient. unable t. t.lerat. longer sequences. Furt.er work is necessary t. confirm t.ese findings in hyperacut. st.oke.

关键词：磁共振成像急性卒中弥散加权成像 FLAIR 血管损伤临床诊断成像时间人为因素

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萃取剂对泥炭腐植酸性质和微量元素的影响

萃取剂对泥炭腐植酸性质和微量元素的影响