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Purification and characterization of glyceraldehyde-3-phosphate dehydrogenase from saline strain Idiomarina loihiensis

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Advances in Biological Chemistry 2013年第2期3卷 170-176页

作者： ilham mardad Tarik Baibai Emna Ammar Abdelaziz Soukri Laboratoire de Physiologie et Génétique Moléculaire Facultédes Sciences Ain ChockUniversitéHassan II-Ain ChockCasablancaMorocco URétude et Gestion des Environnements Urbains et Cotiers LARSENUniversitéde Sfaxécole Nationale d’Ingénieurs de SfaxTunisia

Idiomarina loihiensis was isolated from the salt works in Sfax (Tunisia), until now, the characterization of the GAPDH phosphorylante was never studied. Here, we report the isolation and the biochemical characterization of glyceralehyde-3-phosphate dehydrogenase (GAPDH) fromI. loihiensis saline’s bacteria on the basis of the apparent native and subunit molecular weights, physico-chemical and kinetic characterizations. The purification method consisted of two steps, ammonium sulfate fractionation followed by one chromatographic step, namely dye-affinity on Blue Sepharose CL-6B. Polyclonal antibodies against the purified enzyme were used to recognize theI. loihiensis GAPDH by Western blotting. The optimum pH of the purified enzyme was 8.5. Studies on the effect of temperatures revealed an enzyme increasing activity of about 45?C. The molecular weight of the purified enzyme was 36 kDa determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Non-denaturing polyacrylamide gels yield a molecular weight of 147 kDa. The Michaelis constants for NAD+ and D-glyceraldehyde-3-phosphate estimated was 19 μM and 3.1 μM, respectively. The maximal velocity of the purified enzyme was estimated to be 2.06 U/mg, approximately 6-fold increase in specific activity and a final yield of approximately 32.5%. The physicochemical properties of this GAPDH, being characterized, could be used in further studies.

关键词： Glyceraldehyde-3-Phosphate Dehydrogenase Idiomarina loihiensis Purification NAD^(+) Kinetics Saline Strain

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Machine Learning and Data Fusion Approach for Elastic Rock Properties Estimation and Fracturability Evaluation

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Energy and AI 2024年第2期16卷 1-13页

作者： Yiwen Gong ilham El-Monier Mohamed Mehana The Ohio State University SimTech LLC Los Alamos National Laboratory

Accurate rock elastic property determination is vital for effective hydraulic fracturing,particularly Young’s modulus due to its link to rock *** study integrates interdisciplinary data for better predictions of elastic modulus,combining data mining,experiments,and calibrated *** used the microstructural insights extracted from rock images for geomechanical facies ***,the petrophysical data and well logs were correlated with shear wave velocity(Vs)and Young’s *** developed a machine-learning workflow to predict Young’s modulus and assess rock fracturability,considering mineral composition,geomechanics,and *** findings indicate that artificial neural networks effectively predict Young’s modulus,while K-Means clustering and hierarchical support vector machines excel in identifying rock and geomechanical *** Microscale thin section analysis in conjunction with fracture modeling enhances our understanding of fracture geometries and facilitates fracturability ***,fracturability is controlled by specific geomechanical facies during initiation and propagation and influenced by continuity of geomechanical facies in small depth *** conclusion,this study demonstrates data mining and machine learning potential for predicting rock properties and assessing fracturability,aiding hydraulic fracturing design optimization through diverse data and advanced methods.

关键词： Young’s modulus prediction fracability evaluation landing point selection rock mechanics machine learning

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Enhanced asphalt dynamic modulus prediction: A detailed analysis of artificial hummingbird algorithm-optimised boosted trees

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Journal of Road Engineering 2024年第2期4卷 224-233页

作者： Ikenna D.Uwanuakwa ilham Yahya Amir Lyce Ndolo Umba Department of Civil Engineering Near East UniversityMersin 10Turkey Faculty of Sciences University of LubumbashiLubumbashiThe Democratic Republic of Congo

This study introduces and evaluates a novel artificial hummingbird algorithm-optimised boosted tree(AHAboosted)model for predicting the dynamic modulus(E*)of hot mix asphalt *** a substantial dataset from NCHRP Report-547,the model was trained and rigorously *** metrics,specifically RMSE,MAE,and R2,were employed to assess the model's predictive accuracy,robustness,and *** benchmarked against well-established models like support vector machines(SVM)and gaussian process regression(GPR),the AHA-boosted model demonstrated enhanced *** achieved R2 values of 0.997 in training and 0.974 in testing,using the traditional Witczak NCHRP 1-40D model *** features such as test temperature,frequency,and asphalt content led to a 1.23%increase in the test R2,signifying an improvement in the model's *** study also explored feature importance and sensitivity through SHAP and permutation importance plots,highlighting binder complex modulus|G*|as a key *** the AHA-boosted model shows promise,a slight decrease in R2 from training to testing indicates a need for further ***,this study confirms the AHA-boosted model as a highly accurate and robust tool for predicting the dynamic modulus of hot mix asphalt concrete,making it a valuable asset for pavement engineering.

关键词： Asphalt Dynamic modulus Prediction Artificial hummingbird algorithm Boosted tree

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Assessing the impact of booster vaccination on diphtheria transmission:Mathematical modeling and risk zone mapping

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Infectious Disease Modelling 2024年第1期9卷 245-262页

作者： ilham Saiful Fauzi Nuning Nuraini Ade Maya Sari Imaniah Bazlina Wardani Delsi Taurustiati Purnama Magdalena Simanullang Bony Wiem Lestari Department of Accounting Politeknik Negeri MalangMalangIndonesia Department of Mathematics Faculty of Mathematics and Natural SciencesInstitut Teknologi BandungBandungIndonesia Center for Mathematical Modeling and Simulation Institut Teknologi BandungBandungIndonesia Study Program of Biology Education Faculty of Education and Teacher TrainingUIN Kiai Haji Achmad Siddiq JemberJemberIndonesia West Java Provincial Health Office West JavaIndonesia Department of Public Health Faculty of MedicineUniversitas PadjadjaranBandungIndonesia Department of Internal Medicine Radboud Institute for Health SciencesRadboud University Medical CentreNijmegenthe Netherlands

The COVID-19 pandemic caused significant disruptions in the healthcare system,affecting vaccinations and the management of diphtheria *** a consequence of these disruptions,numerous countries have experienced a resurgence or an increase in diphtheria *** Java province in Indonesia is identified as one of the high-risk areas for diphtheria,experiencing an upward trend in cases from 2021 to *** analyze the situation,we developed an SIR model,which integrated DPT and booster vaccinations to determine the basic reproduction number,an essential parameter for infectious *** spatial analysis of geo-referenced data,we identified hotspots and explained diffusion in diphtheria case *** calculation of R0 resulted in an R0=1.17,indicating the potential for a diphtheria outbreak in West *** control the increasing cases,one possible approach is to raise the booster vaccination coverage from the current 64.84%to 75.15%,as suggested by simulation ***,the spatial analysis revealed that hot spot clusters were present in the western,central,and southern regions,posing a high risk not only in densely populated areas but also in rural *** diffusion pattern of diphtheria clusters displayed an expansion-contagious *** the rising trend of diphtheria cases and their geographic distribution can offer crucial insights for government and health authorities to manage the number of diphtheria cases and make informed decisions regarding the best prevention and intervention strategies.

关键词： Diphtheria Vaccination Mathematical modeling SIR model Hot spot analysis

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Assessing Moroccan physician knowledge and practices regarding maternal obesity's impact on childhood obesity:Implications for prevention and intervention

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World Journal of Clinical Pediatrics 2024年第2期13卷 146-160页

作者： Imane Douiyeh Jihane Khamlich Naima Nabih Asmae Saih ilham Boumendil Anas Regragui Anass Kettani Amal Safi Laboratory Biochemistry Environment and Agri-food Department of BiologyFaculty of Science and Technics MohammediaHassanⅡUniversity CasablancaMohammedia 28806Morocco Department of Biology Faculty of Sciences Ben M’Sik HassanⅡUniversity of CasablancaCasablanca 20000Morocco Department of Medical ACHAS Association of the Doctors Ain Chock Casablanca MoroccoCasablanca 20400Morocco Department of Medical HassanⅡUniversityCasablanca 20400Morocco

BACKGROUND Childhood obesity is a growing global concern with far-reaching health *** study focuses on evaluating the knowledge and practices of physicians in Morocco regarding the link between maternal obesity and childhood *** the increasing prevalence of childhood obesity worldwide,this issue remains inadequately addressed in the Moroccan *** To assess the awareness and practices of physicians in Morocco concerning the connection between maternal obesity and childhood *** The research encompasses a comprehensive survey of practicing physicians,revealing significant gaps in awareness and practices related to maternal *** Notably,a significant portion of doctors do not provide adequate guidance to overweight pregnant women,highlighting the urgency for targeted educational *** In conclusion,this research illuminates critical areas for improvement in tackling childhood obesity in *** addressing these gaps,fostering awareness,and enhancing medical practices,the healthcare system can contribute significantly to preventing childhood obesity and improving the overall health of future generations.

关键词： Childhood obesity Maternal obesity Management Prevention Screening

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Asthma Attack Revealing Sickle Cell Disease

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Open Journal of Pediatrics 2024年第5期14卷 865-868页

作者： Khaoula Achir ilham Tadmori Moustapha Hida Pediatric Department of CHU Hassan II in Fez Fez Morocco

Sickle cell disease is a genetic disorder characterized by the presence of hemoglobin S (HbS) and a significant reduction in normal hemoglobin A (HbA) in red blood cells. In deoxygenated conditions, HbS molecules polymerize, causing vascular occlusions and hemolysis. Pulmonary complications associated with this disease result from vascular occlusion, ischemia-reperfusion, and inflammation. Literature reports that asthma is observed in 30% to 70% of patients with sickle cell disease. We present the case of a boy whose sickle cell disease diagnosis was established following an asthma attack.

关键词： Sickle Cell Disease Asthma Morbidity

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Kostmann’s Neutropenia: A Case Report

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Open Journal of Pediatrics 2024年第5期14卷 869-872页

作者： Khaoula Achir ilham Tadmori Mounia Lakhdar Idrissi Fatima Zahra Souilmi Mohamed Hbibi Moustapha Hida Pediatric Department of CHU Hassan II in Fez Fez Morocco

Kostmann syndrome, or severe congenital neutropenia, is a rare condition in children marked by a neutrophil count of less than 500/mm3. This congenital agranulocytosis, an autosomal recessive genetic disorder, is often first identified by a neonatal infectious syndrome. The deficiency in neutrophils increases susceptibility to bacterial and fungal infections. Prior to the availability of hematopoietic growth factors, the disease was associated with significant morbidity and early mortality. We present the case of a 17-month-old boy who was admitted to the pediatric emergency department at Hassan II University Hospital in Fes with skin abscesses.

关键词： Kostmann Syndrome Severe Congenital Neutropenia Skin Abscesses

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On the Gauss-Weierstrass Summability of Multiple Trigonometric Series at μ-smoothness Points

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Acta Mathematica Sinica,English Series 2011年第4期27卷 741-746页

作者： Sinem SEZER ilham A. ALIEV Department of Mathematics Akdeniz University 07058 Antalya Turkey

The notion of μ-smoothness points of periodic functions of several variables is introduced and the rate of convergence of the Gauss-Weierstrass means of relevant Fourier series at these points is investigated.

关键词： Multiple Fourier series Fourier transform Gauss-Weierstrass summability modulus ofcontinuity

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Microphase separation of a miscible binary liquid mixture under confinement at the nanoscale

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npj Computational Materials 2019年第1期5卷 766-775页

作者： ilham Essafri Denis Morineau Aziz Ghoufi Institut de Physique de Rennes IPRCNRS-Universitéde Rennes 1UMR CNRS 625135042 RennesFrance

Recent experimental works suggested that the confinement into a cylindrical nanopore induced the microphase separation of a binary liquid,despite the miscible character of its bulk counterpart.A core–shell organization was evidenced such that one of the liquids was strongly anchored to the solid surface whereas the other was confined at the center of the *** the same time,a study based on atomistic simulations suggested a strong heterogeneity and the absence of a *** this work,by refining the solid–liquid interactions to qualitatively reproduce the experimental adsorption isotherms of both single liquids,the microphase separation and the core–shell structure are *** tuning the surface chemistry of the nanopore to mimic hydrophilic and hydrophobic confinement,we show that it is possible to control the structural characteristics of the core–shell *** molecular origin of the microphase separation is then ascribed to the strong hydrogen bonds and a commensurate arrangement between the confining material and both liquids.

关键词： separation liquid binary

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Variation in the life-history traits of a Schilbid catfish,Clupisoma garua (Hamilton, 1822) in the coastal waters of southern Bangladesh

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Chinese Journal of Oceanology and Limnology 2017年第5期35卷 1189-1196页

作者： Muhammad Abu Bakar SIDDIK Md Reaz CHAKLADER Md Abu HANIF Ashfaqun NAHAR ilham ilham Anthony COLE Ravi FOTEDAR Department of Fisheries Biology and Genetics Patuakhali Science and Technology University Patuakhali-8602 Bangladesh Department of Marine Fisheries and Oceanography Patuakhali Science and Technology University Patuakhali-8602 Bangladesh Department of Aquatic Resources and Management Jakarta Fisheries University Jl. A UP Pasar Minggu Jakarta Selatan 12520 Indonesia Department of Environment and Agriculture Curtin University 1 Turner Avenue Bentley VIA 6102 Australia

For the first time, the present study reports the life-history traits, comprising length-frequency distribution （LFD）, sex ratio （SR）, length-weight relationships （LWRs）, condition factors （CFs）, and relative growth （WR）, of Clupisoma garua in the coastal waters of Bangladesh. A total of 150 specimens ranging from 8.60 to 25.20 cm total length （TL） and 4.26 to 128.80 g body weight （BW） were collected using traditional fishing gear from August 2013 to July 2014. The overall sex ratio of males to females in the study did not differ significantly from the expected value of 1：1 （χ2=0.96, P〈0.05） but there were significant sex differences （P〈0.05） in the intercepts and slopes of graphs characterizing traits in C. garua. The calculated b values for the LWRs were 2.955, 2.893 and 2.927 for males, females and combined sexes, respectively, and there was negative allometric growth in all cases （b〈3）. The condition factors （KA, KF, KrR） and relative growth （WR） also did not differ significantly （P〈0.05） between the sexes. This study provides a useful tool for fishery specialists to evaluate the relative condition of fish and to initiate early management strategies and regulations for the sustainable management of the remaining stocks of this species in the entire coastal region of southern Bangladesh.

关键词： size frequency length-weight sex ratio allometric growth Clupisoma garua

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