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Hibernation with rhythmicity:the circadian clock and hormonal adaptations of the hibernating Asiatic toads(Bufo gargarizans)

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Integrative Zoology 2022年第4期17卷 656-669页

作者： Zhigang XIE ibrahim m.ahmad Lirong ZUO Feng XIAO Yongpeng WANG Dongming LI Zhejiang Academy of Agricultural Sciences HangzhouChina College of Chemistry and Life Science Zhejiang Normal UniversityJinhuaChina ministry of Education Key Laboratory of molecular and Cellular Biology Key Laboratory of Animal PhysiologyBiochemistry and Molecular Biology of Hebei ProvinceCollege of Life SciencesHebei Normal UniversityShijiazhuangChina

Hibernation is one of the fundamental strategies in response to cold environmental *** hibernation,the endocrine and circadian systems ensure minimal expenditure of energy for *** circadian rhythms of key hormones,melatonin(mT),corticosterone(CORT),triiodothyronine(T3),and thyroxine(T4),and the underlying molecular regulatory mechanisms of hibernation have been well determined in mammals but not in ***,a terrestrial hibernating species,Asiatic toad(Bufo gargarizans),was employed to investigate the plasma CORT,mT,T3,and T4;and the retina,brain,and liver mRNA expression of the core clock genes,including circadian locomotor output cycles kaput(Clock),brain and muscle ARNT-like 1(Bmal1),cryptochrome(Cry)1 and 2,and period(Per)1 and 2,at 7-time points over a 24-h period under acute cold(1 day at 4℃),and hibernation(45 days at 4℃).Our results showed that the circadian rhythms of the core clock genes were rather unaffected by acute cold exposure in the retina,unlike the brain and *** contrast,during hibernation,the liver clock genes displayed significant circadian oscillations,while those in the retina and brain stopped ***,plasma CORT expressed circadian oscillations in both groups,and T3 in acute cold exposure group,whereas T4 and mT did *** results reveal that the plasma CORT and the liver sustain rhythmicity when the brain was not,indicating that the liver clock along with the adrenal clock synergistically maintains the metabolic requirements to ensure basic survival in hibernating Asiatic toads.

关键词： Asiatic toad circadian rhythm clock genes cold exposure hibernation metabolic hormones

Physiological but not morphological adjustments along latitudinal gradients in a human commensal species,the Eurasian tree sparrow

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Integrative Zoology 2023年第5期18卷 891-905页

作者： Qian ZHANG mo LI Yuan YIN Shiyong GE Danjie LI ibrahim m.ahmad Ghulam NABI Yanfeng SUN Xu LUO Dongming LI Hebei Key Laboratory of Animal Physiology Biochemistry and Molecular BiologyCollege of Life SciencesHebei Normal UniversityShijiazhuangChina Key Laboratory for ConservingWildlife with Small Populations in Yunnan Faculty of Biodiversity and ConservationSouthwest Forestry UniversityKunmingChina Ocean College Hebei Agricultural UniversityQinhuangdaoChina Hebei Collaborative Innovation Center for Eco-Environment Hebei Normal UniversityShijiazhuangChina

Human commensal species take advantage of anthropogenic conditions that are less likely to be challenged by the selective pressures of natural *** morphological and physiological phenotypes can therefore dissociate from habitat *** how these species adjust their morphological and physiological traits across latitudinal gradients is fundamental to uncovering the eco-physiological strategies underlying coping ***,we studied morphological traits in breeding Eurasian tree sparrows(ETSs;Passer montanus)among low-latitude(Yunnan and Hunan)and middle-latitude(Hebei)localities in *** then compared body mass;lengths of bill,tarsometatarsus,wing,total body,and tail feather;and baseline and capture stress-induced levels of plasma corticosterone(CORT)and the metabolites including glucose(Glu),total triglyceride(TG),free fatty acid(FFA),total protein,and uric acid(UA).None of the measured morphological parameters varied with latitude except in the Hunan population,which demonstrated longer bills than those in other ***-induced CORT levels significantly exceeded baseline levels and decreased with increasing latitude,but total integrated CORT levels did not vary with *** stress-induced significantly increased Glu levels and decreased TG levels,independent of ***,the Hunan population had significantly higher baseline CORT,baseline and stress-induced FFA levels,but lower UA levels,which differed from other *** results suggest that rather than morphological adjustments,physiological adjustments are mainly involved in coping mechanisms for middle-latitude adaptation in *** is worth investigating whether other avian species also exhibit such dissociation from external morphological designs while depending on physiological adjustments.

关键词： capture stress corticosterone human commensal species latitudinal gradients plasma metabolites

Seasonal variations in gonad morphology and hypothalamic GnRH-I and GnIH in Eurasian Tree Sparrow,a multi-brooded passerine

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Avian Research 2022年第3期13卷 369-378页

作者： Limin Wang Ghulam Nabi Li Zhang Dan Liu mo Li Juyong Li Kaixin Shi ibrahim m.ahmad Yuefeng Wu John C.Wingfield Dongming Li ministry of Education Key Laboratory of molecular and Cellular Biology Key Laboratory of Animal PhysiologyBiochemistry and Molecular Biology of Hebei ProvinceCollege of Life SciencesHebei Normal UniversityShijiazhuang050024China Department of Neurobiology Physiology and BehaviorUniversity of Californi1DavisCAUSA

The hypothalamic-pituitary-gonadal(HPG)axis ubiquitously regulates seasonal reproduction,following the rhythmicity of a suite of environmental *** display prominent seasonal variations in gonad size regulated by two hypothalamic peptides,gonadotropin-releasing hormone-1(GnRH-I)and gonadotropin-inhibiting hormone(GnIH).How multi-brooded avian species adjust GnRH-I and GnIH to regulate gonadal morphology seasonally remains largely ***,we studied the variations in the hypothalamic immunoreactivity(ir)of GnRH-I and GnIH,gonadal proliferation,and apoptosis in a typical multi-brooded species,the Eurasian Tree Sparrow(Passer montanus),across the pre-breeding(PB),first breeding(FB),second breeding(SB),pre-basic molt(Pm),and wintering stages(WS).Our results showed that both sexes had higher preoptic area(POA)-GnRH-I-ir but lower paraventricular nucleus(PVN)-GnIH-ir neurons during the breeding stages(FB and SB)relative to other life-history stages,with no significant differences between two *** testes and ovaries were significantly larger during the breeding *** volume increased during the two broods due to anincreased diameter of seminiferous ***,there were more testicular apoptotic cells in PB and WS stages than in breeding stages,and in PB stage than in Pm *** had higher POA-GnRH-I expression than females during the breeding stages,but both sexes had comparable PVN-GnIH expression throughout the annual *** sexes of the sparrows may undergo a similar pattern of life-history stage-dependent variation in the hypothalamic GnRH-I,GnIH,and gonadal morphology,except that during breeding stages,males may display higher expression of POA-GnRH-I relative to *** higher expression of POA-GnRH-I-ir in breeding male sparrows may be critical for male-dependent parental care.

关键词： Eurasian Tree Sparrow Gonads Hypothalamus Reproduction Seasonality

Effects of constant light and dark conditions on the locomotor activity,body mass,and body temperature rhythms of Eurasian Tree Sparrows(Passer montanus)

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Avian Research 2023年第3期14卷 435-442页

作者： Lirong Zuo ibrahim mahmad Yuanyuan Liu Limin Wang Shu Fang Dongming Li Hebei Key Laboratory of Animal Physiology Biochemistry and Molecular BiologyCollege of Life SciencesHebei Normal UniversityShijiazhuang050024Hebei ProvinceChina Hebei Collaborative Innovation Center for Eco-Environment Hebei Normal UniversityShijiazhuang050024China

Light is an essential environmental cue influencing the endogenous circadian clocks that regulate behavioral and physiological processes in *** extensive research on the circadian rhythms of avian behavior and physiology,the mechanisms by which they adapt and adjust to abnormal photoperiod conditions,such as artificial light,have yet to be fully ***,the circadian rhythms of the body mass,activity,and core and peripheral body temperatures of the Eurasian Tree Sparrow(Passer montanus,ETS)-a diurnal passerine-were examined under three different lighting conditions:constant light(LL),constant darkness(DD),and normal light-dark cycles(LD).Our results showed that the activity,body mass,core,tarsometatarsus,and eye temperatures of the ETSs varied significantly with treatment,time,and the interaction between treatment and time;however,there was no significant change in body mass in response to the interaction between time and *** LD conditions,the ETSs exhibited significant circadian rhythms in activity and core,tarsometatarsus,and eye *** LL conditions,only the core temperature exhibited significant *** DD conditions,there were no significant circadian rhythms in activity,body mass,and eye temperature;however,the core and tarsometatarsus temperatures remained *** results indicate that the core body temperature rhythm of ETS is endogenous and can be maintained under abnormal *** contrast,the activity and body surface temperature,uncoupled from the core body temperature under abnormal photoperiod conditions,strongly depend on *** results differ from those of other diurnal avian species,suggesting a unique coping mechanism of ETS in response to an abnormal photoperiod.

关键词： Eurasian Tree Sparrow Activity Body mass Body temperature Circadian rhythm

mHD stagnation point flow of nanofluid with SWCNT and mWCNT over a stretching surface driven by Arrhenius kinetics

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Applied mathematics(A Journal of Chinese Universities) 2022年第3期37卷 366-382页

作者： Sohail Nadeem Shafiq ahmad Alibek Issakhov ibrahim m.Alarifi Department of mathematics Quaid-I-Azam UniversityIslamabad 44000Pakistan Al-Farabi Kazakh National University av.al-Farabi 71050040AlmatyKazakhstan Department of mechanical and Industrial Engineering College of engineering Majmaah UniversityAl-Majmaah 11952RiyadhSaudi Arabia

The intention of the current research is to address the conclusion of non-isothermal heterogeneous reaction on the stagnation-point flow of SWCNT-engine oil and mWCNT-engine oil nanofluid over a shrinking/stretching ***,exemplify the aspect of heat and mass transfer the upshot of magnetohydrodynamics(mHD),thermal radiation,and heat generation/absorption coefficient are *** bvp4 c from matlab is pledged to acquire the numerical explanation of the problem that contains nonlinear system of ordinary differential equations(ODE).The impacts of miscellaneous important parameters on axial velocity,temperature field,concentration profile,skin friction coefficient,and local Nusselt number,are deliberated through graphical and numerically erected tabulated *** solid volume fraction diminishes the velocity distribution while enhancing the temperature ***,the rate of shear stress declines with increasing the magnetic and stretching parameter for both SWCNT and mWCNT.

关键词： heat generation surface reaction CNTs based nanofluid stretching/shrinking sheet thermal radiation

Solution approximations for a mathematical model of relativistic electrons with beta derivative

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Applied mathematics(A Journal of Chinese Universities) 2024年第3期39卷 469-485页

作者： ibrahim Yalcinkaya Orkun Tasbozan Ali Kurt Hijaz ahmad Department of mathematics and Computer Sciences Faculty of ScienceNecmettin Erbakan UniversityKonyaTurkey Department of mathematics Faculty of Science and LiteratureHatay Mustafa Kemal UniversityHatayTurkey Department of mathematics Faculty of SciencePamukkale UniversityDenizliTurkey Near East University Operational Research Center in HealthcareTRNC Mersin 10Nicosia99138Turkey Department of mathematics Faculty of ScienceIslamic University of MadinahMadinahSaudi Arabia

The main aim of this paper is to obtain the exact and semi-analytical solutions of the nonlinear Klein-Fock-Gordon(KFG)equation which is a model of relativistic electrons arising in the laser thermonuclear fusion with beta *** this purpose,both the modified extended tanh-function(mETF)method and the homotopy analysis method(HAm)are *** applying the mETF the chain rule for beta derivative and complex wave transform are used for obtaining the exact *** advantage of this procedure is that discretization or normalization is not *** applying the mETF,the exact solutions are ***,by applying the HAm semi-analytical results for the considered equation are *** HAm?curve gives us a chance to find the suitable value of the for the convergence of the solution ***,comparative graphical representations are given to show the effectiveness,reliability of the *** results show that the m ETF and HAm are reliable and applicable tools for obtaining the solutions of non-linear fractional partial differential equations that involve beta *** study can bring a new perspective for studies on fractional differential *** the other hand,it can be said that scientists can apply the considered methods for different mathematical models arising in physics,chemistry,engineering,social sciences and *** involves fractional *** the results may cause a new insight who studies on relativistic electron modelling.

关键词： Klein-Fock-Gordon(KFG)equation beta derivative semi-analytical solution exact solution

An efficient nano-adsorbent via surfactants/dual surfactants assisted ultrasonic co-precipitation method for sono-removal of monoazo and diazo anionic dyes

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Chinese Journal of Chemical Engineering 2021年第12期34卷 225-236页

作者： marwa m.ibrahim Department of Chemistry Faculty of EducationAin Shams UniversityRoxy 11711CairoEgypt

To preserve the environment for civilization,we should remove the pollutants like toxic dyes by friendly and cost efficacious *** this study,the effect of surfactants or mixed surfactants on physicochemical,optical and adsorption properties of ternary mixed oxide CeO_(2)-ZrO_(2)-Al_(2) O_3(CZA) are *** ternary mixed oxide CZA was prepared by surfactants or mixed surfactants assisted ultrasonic coprecipitation *** physicochemical and optical properties are estimated by different techniques like XRD,TEm,EDX,FTIR,S_(BET) and UV-Vis/*** CZA_T and CZA_C have hybrid shapes and high surface *** adsorption properties of ternary mixed oxides adsorbents were characterized by sono-removing anionic dyes such as Congo red(CR) and Remazol red RB-133(RR).The different factors like contact time,different dye concentrations and temperatures also *** kinetics and isotherms applications showed that,the adsorption process was followed pseudo second order kinetics and the Freundlich isotherm ***,the adsorption is spontaneous and endothermic process through the thermodynamic ***,the results showed that the ternary mixed oxide nano-adsorbent(CeO_(2)-ZrO_(2)-Al_(2) O_3) is promising and functional materials for anionic dye sweep from wastewater.

关键词： CeO_(2)-ZrO_(2)-Al_(2)O_3 Surfactants Ultrasonic co-precipitation method Optical properties Wastewater Adsorption

multi-Tier Sentiment Analysis of Social media Text Using Supervised machine Learning

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Computers, materials & Continua 2023年第3期74卷 5527-5543页

作者： Hameedur Rahman Junaid Tariq m.Ali masood ahmad F.Subahi Osamah ibrahim Khalaf Youseef Alotaibi Department of Creative Technologies Air UniversityE-9 Islamabad44230Pakistan Department of Computer Science National University of Modern LanguagesRawalpindiPakistan Department of Computer Science University College of Al JamoumUmm Al-Qura UniversityMakkah21421Saudi Arabia Al-Nahrain Nano-Renewable Energy Research Center Al-Nahrain UniversityBaghdad10072Iraq Department of Computer Science College of Computer and Information SystemsUmm Al-Qura UniversityMakkah21955Saudi Arabia

Sentiment Analysis(SA)is often referred to as opinion *** is defined as the extraction,identification,or characterization of the sentiment from ***,the sentiment of a textual document is classified into binary classes i.e.,positive and ***,fine-grained classification provides a better insight into the *** downside is that fine-grained classification is more challenging as compared to *** the contrary,performance deteriorates significantly in the case of multi-class *** this study,pre-processing techniques and machine learning models for the multi-class classification of sentiments were *** augment the performance,a multi-layer classification model has been *** to similitude with social media text,the movie reviews dataset has been used for the *** machine learning models namely Decision Tree,Support Vector machine,and Naive Bayes models have been implemented for the task of sentiment *** have compared the models of single-layer architecture with multi-tier *** results of multi-tier model have slight improvement over the single-layer ***,multi-tier models have better recall which allow our proposed model to learn more *** have discussed certain shortcomings of the model that will help researchers to design multi-tier models with more contextual information.

关键词： Sentiment analysis machine learning multi-class classification svm decision tree naive bayes

molecular characterization of yolk proteins in the female crab Neptunus pelagicus(***-Edwards,1861) from the mediterranean Sea of Alexandria,Egypt

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Journal of Oceanology and Limnology 2020年第2期38卷 438-453页

作者： ibrahim G ELGHAZALY m Department of Zoology Faculty of ScienceAlexandria UniversityAlexandria 21547Egypt Department of Zoology Faculty of ScienceDamnhour UniversityEl Behara 22516Egypt

This study aimed to characterize the morphological changes in the ovary of the female crab Neptunus pelagicus and to identify specific fractions of vitelloginin and vitelline molecules during primary and secondary *** of the blue crab were collected from the mediterranean Sea of Alexandria monthly during *** and oocytes in primary and secondary vitellogenesis were detached and treated for histological *** polyacrylamide gel electrophoresis(PAGE)Bis-Tris Gels was applied to identify vitelloginin(VN)and vitelline(VL)*** Analyses were done by *** initial degenerate primers were built regarding the conserved amino acid domains of the yolk *** and secondary vitellogeneses consisted of 8 *** fraction with molecular weight 550 kDa was identified in the hemolymph in secondary *** protein fractions(VLI&VLII)were identified in secondary vitellogenic *** electrophoresis performed with extract of stage I oocyte showed two protein fractions with molecular weights 550 kDa and 460 *** stage II and III oocyte,4 subunits were presented of 180,195,140 and 120 kDa in VLI and 2 subunits with molecular weight of 110 kDa and 95 kDa in *** two fractions in stage V oocyte presented with molecular weights of 380 kDa and 360 *** blot analysis proved that both fractions were of four major polypeptide subunits with molecular weight of 180,125,90 and 85 kDa in each of the two *** hybridization signal obtained by the Northern blot was detected in the hepatopancreas during ovarian cycle and in the ovary during secondary *** result of the reverse transcription-polymerase chain reaction(RT-PCR)analysis showed that the mRNA that encodes the C-terminal region of the VN cDNA was found in the ovary in secondary vitellogenesis and in the hepatopancreas.

关键词： Neptunus pelagicus vitellogenesis vitelloginin and vitelline native polyacrylamide gel electrophoresis(PAGE)Bis-Tris Gels PAGE-SDS western blot analysis reverse transcription-polymerase chain reaction(RT-PCR)