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Evaluation of plant seed DNA and botanical evidence for potential forensic applications

Forensic Sciences Research 2020年第1期5卷 55-63页

作者： Cheng-Lung Lee Yi-Hsin Huang ian chsu Henry CLee Department of Criminal Investigation Taiwan Police CollegeTaiwanChina Crime Scene Theater Qatar Police CollegeDohaQatar Institute of Business and Management National Chiao Tung UniversityTaiwanChina Department of Biomedical Engineering and Environmental Sciences National Tsing Hua UniversityTaiwanChina Forensic Science Program University of New HavenWest HavenCTUSA

Seeds,the reproductive organs of plants,are common as trace evidence from crime *** evidence could be grouped into several categories based on the types of crimes they are associated with,including child abuse,homicides and *** commonly,seeds are examined microscopically and identified to the plant species level to show a linkage between persons and *** recently,forensic researchers have evaluated the poten-tial for extracting and typing DNA from seeds to further individualize the *** a model system,tomato seeds were examined microscopically after different cooking treat-ments and assessed for the potential to DNA type seeds for variety identification.A sufficient quantity and quality of DNA were recovered from uncooked,digested and undigested tomato seeds for amplified fragment length polymorphism(AFLP)analysis;however,any form of cooking destroyed the seed DNA.A simple microscopic analysis was able to distin-guish between a cooked tomato seed versus an uncooked *** article is intended to provide an overview of case examples and current techniques for the forensic examination of seeds as plant-derived evidence.

关键词： Forensic sciences forensic botany tomato seed DNA extraction crime scene non-human evidence AFLP STR

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