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Rice pollen-specific OsRALF17 and OsRALF19 are essential for pollen tube growth

植物学报:英文版 2023年第9期65卷 2218-2236页

作者： Eui‐Jung Kim Ji‐Hyun Kim woo‐Jong Hong Eun Young Kim Myung‐Hee Kim Su Kyoung lee Cheol woo Min Sun Tae Kim Soon Ki Park Ki‐Hong Jung Yu‐Jin Kim Graduate School of Green Bio‐Science&Crop Biotech Institute Kyung Hee UniversityYongin 17104Republic of Korea Department of Life Science and Environmental Biochemistry Life and Industry Convergence Research InstitutePusan National UniversityMiryang 50463Republic of Korea Department of Smart Farm Science Kyung Hee UniversityYongin 17104Republic of Korea School of Applied Biosciences Kyungpook National UniversityDaegu 41566Republic of Korea Genomics Division Department of Agricultural Bio‐ResourcesNational Institute of Agricultural SciencesRural Development AdministrationJeonju 54874Republic of Korea Department of Plant Bioscience Pusan National UniversityMiryang 50463Republic of Korea Research Center for Plant Plasticity Seoul National UniversitySeoul 08826Republic of Korea

Pollen tube growth is essential for successful double fertilization, which is critical for grain yield in crop plants. Rapid alkalinization factors(RALFs) function as ligands for signal transduction during fertilization. However, functional studies on RALF in monocot plants are ***, we functionally characterized two pollen-specific RALFs in rice(Oryza sativa) using multiple clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9-induced loss-of-function mutants,peptide treatment, expression analyses, and tag reporter lines. Among the 41 RALF members in rice, OsRALF17 was specifically expressed at the highest level in pollen and pollen *** applied OsRALF17 or OsRALF19peptide inhibited pollen tube germination and elongation at high concentrations but enhanced tube elongation at low concentrations, indicating growth regulation. Double mutants of OsRALF17 and OsRALF19(ralf17/19) exhibited almost full male sterility with defects in pollen hydration, germination, and tube elongation,which was partially recovered by exogenous treatment with OsRALF17 peptide. This study revealed that two partially functionally redundant OsRALF17 and OsRALF19 bind to Oryza sativa male-gene transfer defective 2(OsMTD2) and transmit reactive oxygen species signals for pollen tube germination and integrity maintenance in rice. Transcriptomic analysis confirmed their common downstream genes, in osmtd2 and ralf17/19. This study provides new insights into the role of RALF, expanding our knowledge of the biological role of RALF in regulating rice fertilization.

关键词： CRISPR-Cas9 CrRLK1L monocot Oryza sativa pollen tube growth rapid alkalinization factor(RALF)

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Estimation of genetic parameters on growth characteristics of a 35-year-old Pinus koraiensis progeny trial in South Korea

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Journal of Forestry Research 2021年第5期32卷 2227-2236页

作者： Kyungmi lee In Sik Kim Seok woo lee Division of Forest Tree Improvement Department of Forest BioresourcesNational Institute of Forest ScienceSuwonRepublic of Korea

The effects of genetic and environmental factors on Pinus koraiensis growth were studied based on a35 year-old progeny trial composed of open-pollinated offspring of twenty-one plus ***,DBH and volume of the offspring was analyzed using restricted maximum likelihood/best linear unbiased prediction in mixed model *** site and family effects on the three traits were *** distinct growth of offspring by site with disparate climates corroborated the importance of planting species in suitable *** differences by family was significant,emphasizing the importance of identifying families with either superior or inferior *** parental ranking was assigned in the sites,inferring the breeding value of each plus *** estimates of individual heritability(h_(i)^(2))of height,DBH and volume growth were 0.169-0.645,0.108-0.331 and 0.129-0.343 respectively,with higher h_(i)^(2) of the height than DBH on each *** of variance of genetic effect was higher with DBH in some cases,indicating the scope for selection is larger for this trait despite the lower heritability compared to *** the variation between families in terms of the performance stability across sites,consideration of the genotype by environment interaction is required in selecting materials to be used in reforestation with Korean pine.A few families with either superior or inferior performance retained their parental ranking for at least a *** families with increased growth on a particular site were identified,indicating their high breeding value and low *** in the genetic performance of the families by site requires delineation of the breeding region of the species.

关键词： Pinus koraiensis Genetic parameters Genotype by environment interaction Tree improvement Progeny trial

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Gastric cancer surgery in South Korea: Past, present, and future

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Chinese Journal of Cancer Research 2023年第6期35卷 627-635页

作者： Sung Gon Kim Sang Eok lee Bang wool Eom Hong Man Yoon Young-woo Kim Keun Won Ryu Department of Surgery Konyang University HospitalDaejeon 35365Republic of Korea Center for Gastric Cancer Research Institute&HospitalNational Cancer CenterGoyang-si 410-769Republic of Korea

Gastric cancer remains a significant global health concern and its surgical management approaches have undergone significant changes in South Korea and worldwide. Subtotal or total gastrectomy with D2 lymph node dissection is well established as a standard surgical procedure for gastric cancer. With the active implementation of cancer screening in South Korea, the proportion of early gastric cancer cases has significantly increased over the past few decades, leading to a steady increase in the survival rate among patients. Furthermore, recent advances in surgical instruments and techniques have made minimally invasive surgery increasingly prevalent, not only for early but also for advanced gastric cancer. We aim to provide a comprehensive overview of the evolution and current status of gastric cancer surgery in South Korea.

关键词： Function-preserving surgery gastric cancer minimally invasive surgery surgery

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Electronic and optical properties of CdS/CdZnS nanocrystals

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Chinese Physics B 2012年第8期21卷 441-448页

作者： A.John Peter Chang woo lee epartment of Chemical Engineering and Green Energy Center College of EngineeringKyung Hee University1 SeochunGihungYonginGyeonggi 446-701S.Korea Department of Physics Govt.Arts CollegeMelurMadurai-62510India Department of Chemical Engineering and Green Energy Center College of EngineeringKyung Hee University1 SeochunGihungYonginGyeonggi 446-701S.Korea

Cd1-xZnxS nanocrystals are prepared by a co-precipitation method with different atomic fractions of Zn. The texture, structural transformation and optical properties with increasing x value in Cd1-xZnxS are studied with scanning electron microscopy, electron diffraction patterning, and absorption spectra respectively. Quantum confinement in a strained CdS/Cd1-xZnxS related nanodot with various Zn content values is investigated theoretically. Binding energies on exciton bound CdS/CdxZn1-xS quantum dot are computed, with consideration of the internal electric field induced by the spontaneous and piezoelectric polarizations, and thereby the interband emission energy is calculated as a function of the dot radius. The optical band gap from the UV absorption spectrum is compared with the interband emission energy computed theoretically. Our results show that the average diameter of composite nanoparticles ranges from 3 nm to 6 nm. The X-ray diffraction pattern shows that all the peaks shift towards the higher diffracting angles with an increase in Zn content. The lattice constant gradually decreases as the Zn content increases. The strong absorption edge shifts towards the lower wavelength region and hence the band gap of the films increases as the Zn content increases. The values of the absorption edge are found to shift towards the shorter wave length region and hence the direct band gap energy varies from 2.5 eV for the CdS film and 3.5 eV for the ZnS film. Our numerical results are in good agreement with the experimental results.

关键词： quantum dots quasicrystals semiconductor

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A Routing Protocol for Urban Vehicular Multi-hop Data Delivery

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Chinese Journal of Electronics 2016年第2期25卷 348-356页

作者： CHANG Sang-woo lee Sang-sun Department of Electronic Engineering Hanyang University

Vehicular ad-hoc network(VANET) has distinctive environments such as highly dynamic topology, frequently disconnected network, hard delay constraints for safety-related application, and various communications environments based on highway or urban traffic scenarios. Therefore, development of a suitable routing protocol that considers these characteristics of VANET should be needed. In this paper we propose the improved distance-based VANET routing protocol in urban traffic environments. We apply approaches to multi-hop broadcast scheme for reliable packet dissemination in the intersection and stable route decision scheme based on the adaptive waiting time. The performance is evaluated under simulation which is implemented with the Manhattan urban traffic model. The results show the improved performance as compared with other protocols.

关键词： Distance-based routing protocol Vehicle-to-vehicle communication Waiting time Urban traffic environments Vehicular ad-hoc network(VANET)

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Radiative life time of an exciton confined in a strained GaN/Ga_(1-x)Al_xN cylindrical dot:built-in electric field effects

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Chinese Physics B 2011年第7期20卷 367-372页

作者： Chang woo lee A.John Peter Department of Chemical Engineering and Green Energy Center College of EngineeringKyung Hee University1 SeochunGihungYonginGyeonggi 446-701Republic of Korea Department of Physics Government Arts and Science CollegeMelur-625106India

The binding energy of an exciton in a wurtzite GaN/GaAlN strained cylindrical quantum dot is investigated theoretically. The strong built-in electric field due to the spontaneous and piezoelectric polarizations of a GaN/GaAlN quantum dot is included. Numerical calculations are performed using a variational procedure within the single band effective mass approximation. Valence-band anisotropy is included in our theoretical model by using different hole masses in different spatial directions. The exciton oscillator strength and the exciton lifetime for radiative recombination each as a function of dot radius have been computed. The result elucidates that the strong built-in electric field influences the oscillator strength and the recombination life time of the exciton. It is observed that the ground state exciton binding energy and the interband emission energy increase when the cylindrical quantum dot height or radius is decreased, and that the exciton binding energy, the oscillator strength and the radiative lifetime each as a function of structural parameters （height and radius） sensitively depend on the strong built-in electric field. The obtained results are useful for the design of some opto-photoelectronic devices.

关键词： quantum dot donor bound excitons oscillator strength

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Analgesic efficacy of median nerve stimulation in mice with chemotherapy-induced peripheral neuropathy via modulation of brain-derived neurotrophic factor expression

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中医杂志:英文版 2023年第4期43卷 686-694页

作者： Dong-wook Kang Jae-Gyun Choi Hee Ju Song Jaehyuk Kim Miae lee Taehee Kim Suk-Yun Kang Yeonhee Ryu Hwa Seung Yoo Jin Sun lee Jin Bong Park Sang Do lee Hyun-woo Kim Department of Physiology and Medical Science College of Medicine and Brain Research InstituteChungnam National UniversityDaejeon 35015Republic of Korea KM Fundamental Research Division Korea Institute of Oriental Medicine Daejeon 34054Republic of Korea East-West Cancer Center Dunsan Korean Medicine Hospital of Daejeon UniversityDaejeon 35234Republic of Korea Department of Surgery Chungnam National University HospitalDaejeon 35015Republic of Korea Laboratory of Veterinary Pharmacology College of Veterinary Medicine and Research Institute for Veterinary ScienceSeoul National University1 Gwanak-roGwanak-guSeoul 08826Republic of Korea

OBJECTIVE:Chemotherapeutic agents such as docetaxel(DTX)can trigger chemotherapy-induced peripheral neuropathy(CIPN),which is characterized by unbearable *** study was designed to investigate the analgesic effect and related neuronal mechanism of low-frequency median nerve stimulation(LFMNS)on DTX-induced tactile hypersensitivity in ***:To produce CIPN,DTX was administered intraperitoneally 4 times,once every 2 d,to male ICR *** was performed on the wrist area,and the pain response was measured using von Frey filaments on both hind *** blot and immunofluorescence staining were performed using dorsal root ganglion and spinal cord samples to measure the expression of brainderived neurotrophic factor(BDNF).RESULTS:Repeated LFMNS significantly attenuated the DTX-induced abnormal sensory response and suppressed the enhanced expression of BDNF in the DRG neurons and spinal dorsal ***:LFMNS might be an effective nonpharmaceutical option for treating patients suffering from CIPN via regulating the expression of peripheral and central BDNF.

关键词： analgesia brain-derived neurotrophic factor chemotherapy-induced peripheral neuropathy docetaxel nerve stimulation

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Advancing Li-ion storage performance with hybrid vertical carbon/Ni_(3)S_(2)-based electrodes

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Journal of Energy Chemistry 2022年第4期31卷 8-18页

作者： Neelakandan M.Santhosh Nitheesha Shaji d Petra Stražar Gregor Filipič Janez Zavašnik Chang Won Ho Murugan Nanthagopal Chang woo lee UrošCvelbar Department of Gaseous Electronics(F6) Jožef Stefan InstituteJamova cesta 39SI-1000 LjubljanaEUSlovenia Jožef Stefan International Postgraduate School Jamova cesta 39SI-1000 LjubljanaEUSlovenia Faculty of Mathematics and Physics University of LjubljanaJadranska cesta 19SI-1000 LjubljanaEUSlovenia Department of Chemical Engineering(Integrated Engineering) College of EngineeringKyung Hee University1732 Deogyeong-daeroGiheungYonginGyeonggi 17104South Korea Center for the SMART Energy Platform College of EngineeringKyung Hee University1732 Deogyeong-daeroGiheungYonginGyeonggi 17104South Korea

Conversion-reaction induced charge storage mechanisms of transition metal sulphides have received considerable interest in designing high-capacity electrodes for electrochemical energy storage ***,their low conductivity and structural degradation during cycling limit their applications as energy storage devices.A combination of different nickel sulphide phases tailored with carbon nanostructures is suggested to address these ***,a facile,two-step approach is demonstrated for fabricating a hybrid electrode,consisting of trinickel disulphide(Ni_(3)S_(2))formed on a metallic Ni nanoparticle supported by vertical carbon nanotubes(VCN)backbone in the form Ni_(3)S_(2)/Ni@***_(3)S_(2)/Ni@VCN electrodes were tested as anode for lithium-ion batteries,and the electrode featured outstanding lithiumstorage capabilities with a high reversible capacity(1113 m Ah g^(-1) after 100 cycles at 100 m A g^(-1)),excellent long-term cycling stability(770 m Ah g^(-1) after 500 cycles at 200 m A g^(-1)),and good rate *** resulting electrode performance is one of the best Li-ion storage capabilities in the Ni_(3)S_(2)-type anode materials described.A unique “broccoli-like”structure of polycrystalline Ni_(3)S_(2)capped on conductive VCN backbone helps the interface storage process and boosts lithium storage performance.

关键词： Ni_(3)S_(2) Vertical carbon nanostructures Hierarchical structures Binder-free electrode Lithium-ion batteries

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Experimental Investigation and Numerical Analysis to Develop Low-Energy Large-Midwater Trawls

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China Ocean Engineering 2017年第6期31卷 700-708页

作者： Jihoon lee Taeho KIM Chun-woo lee Subong PARK Division of Marine Technology Chonnam National University Yeosu 550-749 Korea Division of Marine Production System Management Pukyong National University Busan 608-737 Korea Department of Fisheries Physics Pukyong National University Busan 608-737 Korea

Fuel consumption in fisheries is a primary concern because of its effects on the environment and the costs incurred by fishermen. Many studies have been conducted to reduce the fuel consumption in fishing operations. Fuel consumption due to fishing gear during a fishing operation is generally related to the hydrodynamic resistance on the gear. This means that fuel consumption is proportional to the drag created by the towing speed. Based on numerical methods, this study suggests a new approach to reduce fuel consumption in fisheries. The results of the simulation are in good agreement with those of model experiments. The total as well as partial resistance forces on the gear are calculated by simulation. The simulation results suggest improved materials and gear structure for reducing the hydrodynamic forces on the gear while maintaining gear performance. The method for assessing the gear performance involves measuring the height and width of the net mouth. Furthermore, this study investigates the efficiency of a low-energy trawl from an economic point of view. The findings of this study will be useful in reducing greenhouse gas （GHG） emissions in fishing operations, and thereby contribute toward lowering fishing costs by saving fuel.

关键词： low-energy fishing gear numerical methods greenhouse gasesemission hydrodynamic resistance fuelconsumption

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Intra and extra pelvic multidisciplinary surgical approach of retroperitoneal sarcoma:Case series report

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World Journal of Clinical Cases 2022年第27期10卷 9693-9702页

作者： Heekyoung Song Jung Hwan Ahn Yuyeon Jung Jae Yeon woo Jimin Cha Yang-Guk Chung Keun Ho lee Department of Obstetrics and Gynecology Incheon St.Mary’s HospitalCollege of MedicineThe Catholic University of KoreaSeoul 06591South Korea Department of Obstetrics and Gynecology Seoul St.Mary’s HospitalCollege of MedicineThe Catholic University of KoreaSeoul 06591South Korea Department of Orthopaedic Surgery Seoul St.Mary’s HospitalCollege of MedicineThe Catholic University of KoreaSeoul 06591South Korea

BACKGROUND Retroperitoneal sarcoma(RPS) is a rare malignancy arising from mesenchymal cells that most commonly presents as an abdominal mass and is associated with poor *** several studies have assessed the survival benefits of wide excision,few have reported detailed methods for achieving wide excision in patients with *** To describe our experience with multidisciplinary surgical resection of RPS using intra-and extra-pelvic *** Multidisciplinary surgery is an anatomical approach that combines intra-and extra-peritoneal access within the same surgery to achieve complete RPS *** retrospective review of the records of patients who underwent multidisciplinary surgery for RPS analyzed surgical and survival *** Eight patients underwent 10 intra-and extra-pelvic surgical resections,and their median mass size was 12.75 cm(range,6-45.5 cm).Using an intrapelvic approach,laparoscopy-assisted surgery was performed in four cases and laparotomy surgery in *** an extrapelvic approach,ilioinguinal and posterior approaches were used in four cases each,and the prone position and midline skin incision were shared in *** patients’ RPS masses were removed completely,and four achieved R0 resection through intra-and extra-pelvic *** median estimated blood loss was 2000 m L(range,300-20000 m L) and the median hospitalization was 12.6 d(range,9-69 d).Reoperation was needed in two patients(one for wound necrosis and the other for bowel perforation and wound necrosis).The median overall survival rate and median progression-free survival were 64.6 and 13.7 mo,*** RPS is therapeutically challenging because of its location and high risk of ***,intra-and extra-pelvic surgical approaches can improve the macroscopic security of the surgical margin.

关键词： Margins of excision Retroperitoneal neoplasms Sarcoma

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