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Analysis of proteomic profiling of mouse embryonic stem cells derived from fertilized, parthenogenetic and androgenetic blastocysts

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Stem Cell Discovery 2011年第1期1卷 1-15页

作者： Xiang-Shun Cui Xing-Hui Shen Chang-Kwon lee Yong-Kook Kang Teruhiko Wakayama Nam-hyung Kim 不详

Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of preimplantation embryos. ESCs exhibit true pluripotency, i.e., the ability to differentiate into cells of all three germ layers in the developing embryo. We used 2-DE MALDI-TOF/TOF to identify differentially expressed proteins among three types of mouse embryonic stem cells (ESCs) derived from ferti-lized, parthenogenetic, and androgenetic (fESC, pESC and aESC, respectively) blastocysts. We detected more than 800 proteins on silver- stained gels of whole protein extracts from each type of ESC. Of these, 52 differentially expressed proteins were identified by the MALDI–TOF/TOF analyzer, including 32 (fESCs vs. pESCs), 28 (fESCs vs. aESCs) and 17 (pESCs vs. aESCs) prominent proteins with significantly higher or lower expression relative to the comparison cells. Among the 32 proteins from fESCs, 12 were significantly increased in expression and 20 were reduced in expression in fESCs com-pared with pESCs. Similarly, 10 of 28 and 8 of 17 proteins were more highly expressed in fESCs and pESCs compared with aESCs, respectively. In contrast, 18 of 28 and 9 of 17 proteins were reduced in expression in fESCs and pESCs compared with aESCs, respectively. Of the eight protein candidates in fESCs, four were in-creased and four were decreased in expression relative to both pESCs and aESCs in the 2-DE analysis. Differential expression of these pro-teins were confirmed by mRNA expression analysis using real time RT-PCR. For three pro-teins, ANXA5, CLIC1 and SRM, Western blot analysis corroborated the expression patterns indicated by the 2-DE results. ANXA5 and CLIC1 were increased in expression and SRM was de-creased in expression in fESCs compared with both pESCs and aESCs. The differentially ex-pressed proteins identified in the present study warrant further investigation towards the goal of their potential application in ESC therapy.

关键词： Protein Profiling Embryonic Stem Cell Parthenogenote Androgenote Fertilization

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Sawdust Cultivation of Lentinus lepideus Using Liquid-Cultured Spawn

Sawdust Cultivation of Lentinus lepideus Using Liquid-Cultur...

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The 7th Korea-China Joint Symposium for Mycology

作者： Keum chul SHIN Nam Kyu KIM Byung Ju CHO Jong Kyu lee Kangwon National University Kangwon National University Kangwon National University Kangwon National University

＜正＞To obtain the basic data for large scale cultivation of Lentinus lepideus, mushroom cultivation using Korean pine （Pinus koraiensis） sawdust and liquid-cultured spawn were carried out. L. lepideus showed the most favorable growth in culture medium, BPYM （Brown sugar, Peptone, Yeast extract, and Malt extract）. The optimal culture period and aeration rate

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Extension and Design of Securc Dynamic Updates in Domain Name Systems

Extension and Design of Securc Dynamic Updates in Domain Nam...

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亚太地区通信会议暨光电子通信会议

作者： Young-chul Shim Hee-Won Shim Man-Hee lee OK-Hwan Byeon Hong-Ik University Dept.of Computer Engineering 72-1 Sangsudong Mapogu Seoul Korea 121-791 Hong-Ik University Dept.of Computer Engineering 72-1 Sangsudong Mapogu Seoul Korea 121-791 ETRl Supercomputer Center Taejon Korea ETRl Supercomputer Center Taejon Korea

Recent IETF proposals for the secure dynamic updatesin the DNS dictate that any DNS update requests arerestricted to the same zone as the requester. This limitsthe flexibility and applicability of many servers such asCA servers. In this paper we propose to use two morebits in the DNS header section to define the scope of thezones over which a dynamic update key has an updateaccess right. These bits are called scope control *** also describe how requests for dynamic updates canbe generated and processed in the resolver and the DNSservers using these scope control bits

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Formation of ZSM-5 on Silicon Carbide Fibers for Catalytic Support

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材料科学与工程（中英文B版） 2016年第5期6卷 211-217页

作者： Yoon-joo lee Dong-geun Shin Younghee Kim Woo-teck Kwon Soo-ryong Kim Doh-hyung Riu Energy Materials Center Korea Institute of Ceramic Engineering and Technology 101 Soho-ro Jinju-si Gyeongsangnam-do52851 Korea Ceramic Fiber & Composite Center Korea Institute of Ceramic Engineering and Technology 101 Soho-ro Jinju-sLGyeongsangnam-do 52851 Korea Department of Materials Science and Engineering Seoul National University of Science and Technology 172 Gongreung 2-dongNowon-gu Seoul 139-743 Korea

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Portal vein stenting as a significant risk factor for biliary stricture in adult living donor liver transplantation

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Hepatobiliary & Pancreatic Diseases International 2016年第5期15卷 480-486页

作者： Min-Ho Shin Deok-Bog Moon Sung-Gyu lee Shin Hwang Ki-Hun Kim chul-Soo Ahn Tae-Yong Ha Gi-Won Song Dong-Hwan Jung Gil-Chun Park Young-In Yun Wan-Jun Kim Woo-Hyoung Kang Seok-Hwan Kim Gi-Young Ko Division of Hepatobiliary Surgery and Liver Transplantation Department of SurgeryAsan Medical CenterUniversity of Ulsan College of Medicine88 0lympic-ro 43 gilSongpa-guSeoul 05505Korea Department of Radiology and Research Institute of Radiology Asan Medical CenterUniversity of Ulsan College of Medicine88 0lympic-ro 43 gilSongpa-guSeoul 05505Korea

BACKGROUND: Although perioperative portal vein (PV) stent implantation is an effective treatment for steno-occlusive disease in adult living donor liver transplantation (LDLT) re-cipients, we experienced high incidence of biliary anastomotic strictures (BAS) after PV stenting. In this study, we sought to clarify the relation between BAS and PV stenting and to suggest the possible mechanism of BAS and measures to reduce its in-cidence. METHODS: We retrospectively analyzed 44 LDLT recipients who underwent PV stent implantation across the line of PV anastomosis regardless of the location of steno-occlusion (stent group) and their matched controls (non-stented LDLT recipients, n=131). RESULTS: The incidence of BAS was higher in patients in the stent group than that in the control group (43.2% vs 17.6%, P=0.001). Cumulative 6-month and 1-, 2- and 5-year BAS rates were 31.8%, 34.1%, 41.4% and 43.2%, respectively, in the stent group and 13.0%, 13.8%, 16.1% and 17.8%, respectively, in the control group (P=0.001). Multivariate analysis revealed that PV stenting was an independent risk factor for BAS. CONCLUSIONS: Although PV stent implantation is a reliable treatment modality for steno-occlusive PV in adult LDLT recipients, innovative methods to prevent the PV stent from crossing the line of PV anastomosis may be necessary to reduce the incidence of postoperative BAS.

关键词： portal vein stenting biliary stricture living donor liver transplantation

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Diffusion-weighted imaging of biliopancreatic disorders:Correlation with conventional magnetic resonance imaging

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World Journal of Gastroenterology 2012年第31期18卷 4102-4117页

作者： Nam Kyung lee Suk Kim Gwang Ha Kim Dong Uk Kim hyung Il Seo Tae Un Kim Dae Hwan Kang Ho Jin Jang Department of Radiology Biomedical Research InstitutePusan National University HospitalSchool of MedicinePusan National UniversityBusan 602-739South Korea Department of Internal Medicine Biomedical Research InstitutePusan National University HospitalSchool of MedicinePusan National UniversityBusan 602-739South Korea Department of Surgery Biomedical Research InstitutePusan National University HospitalSchool of MedicinePusan National UniversityBusan 602-739South Korea Department of Radiology Yangsan Pusan National University HospitalSchool of MedicinePusan National UniversityYangsan 626-770South Korea Department of Internal Medicine Yangsan Pusan National University HospitalSchool of MedicinePusan National UniversityYangsan 626-770South Korea Department of Obstetrics and Gynecology Armed Forces Seoul HospitalSeoul 110-200South Korea

Diffusion-weighted magnetic resonance imaging(DWI)is a well established method for the evaluation of intracranial diseases,such as acute *** for extracranial application is more difficult due to physiological motion artifacts and the heterogeneous composition of the ***,thanks to the newer technical development of DWI,DWI has become increasingly used over the past few years in extracranial organs including the abdomen and *** previous studies of DWI have been limited to the evaluation of diffuse parenchymal abnormalities and focal lesions in abdominal organs,whereas there are few studies about DWI for the evaluation of the biliopancreatic *** further studies are needed to determine its performance in evaluating bile duct,gallbladder and pancreas diseases,DWI has potential in the assessment of the functional information on the biliopancreatic tract concerning the status of tissue cellularity,because increased cellularity is associated with impeded diffusion,as indicated by a reduction in the apparent diffusion *** detection of malignant lesions and their differentiation from benign tumor-like lesions in the biliopancreatic tract could be improved using DWI in conjunction with findings obtained with conventional magnetic resonance ***,DWI can be useful for the assessment of the biliopancreatic tract in patients with renal impairment because contrast-enhanced computed tomography or magnetic resonance scans should be avoided in these patients.

关键词： Magnetic resonance imaging Diffusion-weighted imaging Biliary tract Gallbladder Pancreas

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Dynamic Behavior of Bump Foils with Thermal Effect in Air Foil Bearings

Dynamic Behavior of Bump Foils with Thermal Effect in Air Fo...

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第9届全国转子动力学学术讨论会ROTDYN'2010

作者： Yong-Bok lee Jun-Hyeon Jo Kyu Ho Sim Yoon-chul Rhim Energymechanics Center Korea Institute of Science and Technology P.O.Box 131CheongryangSeoulKorea130-650 Yonsei University 134 Sinchon-dongSeodaemun-guSeoulRepublic of Korea 120-749

An air foil bearing is composed of a bump foil,top foil and bearing *** bump foil provides high stiffness and damping. In this study,the dynamics of the bump foil is investigated by FEM(Finite element method)and an experimental *** strain-stress method and triangle elements were used for FEM *** thermal effect was considered by property of foils’ high temperature material *** measure stiffness and damping coefficients of the bump foil considering thermal effects,the high temperature bump foil test setup was *** results of comparing FEM analysis and experiments show that the frictions between the bump foil-the top foil and the bump foil-the bearing housing,and high temperature affect to stiffness and damping coefficients of bump foils.

关键词： Air foil bearing High temperature thermal effect Dynamic coefficient

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Occult micrometastases in colorectal cancer-An immunohistochemical study

Occult micrometastases in colorectal cancer-An immunohistoch...

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第六次全国大肠癌会议暨中日韩大肠癌会议

作者： Seok-Hwan lee Tae-Young Kim Choong Yoon Hoong-Zae Joo Yoon-Wha Kim Kee-hyung lee M.D. Department of Surgery and Pathology Kyung-Hee University Hospital Seoul Korea M.D. Department of Surgery and Pathology Kyung-Hee University Hospital Seoul Korea M.D. Department of Surgery and Pathology Kyung-Hee University Hospital Seoul Korea M.D. Department of Surgery and Pathology Kyung-Hee University Hospital Seoul Korea M.D. Department of Surgery and Pathology Kyung-Hee University Hospital Seoul Korea M.D. Department of Surgery and Pathology Kyung-Hee University Hospital Seoul Korea

＜正＞Purpose : One of the most important prognostic factors in colorectal cancer is lymph node metastasis, which predicts a reduced survival time. Although lymph node metastases were not detected by a conventional hematoxylin-eosin stain technique, 20 to 30 percent of patients fail long-term survival on account of a local or systemic recurrence. Recurrent disease in these patients is believed to develop from occult tumor in lymph nodes. We have conducted an immunohistochemical study with two different antibodies against cytokeratin to identify occult micrometastases in lymph nodes which were diagnosed as tumor negative by conventional histopathology.

关键词： Colorectal cancer Immunohistochemical study Lymph node metastases Occult micrometastases

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Spontaneous liver rupture in a patient with peliosis hepatis:A case report

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World Journal of Gastroenterology 2009年第43期15卷 5493-5497页

作者： Sun-Keun Choi Jun-Shuo Jin Soon-Gu Cho Suk-Jin Choi chul-Soo Kim Yun-Mee Choe Keon-Young lee Department of Surgery Inha University School of MedicineIncheon 400-711South Korea Department of Radiology Inha University School of MedicineIncheon 400-711South Korea Department of Pathology Inha University School of MedicineIncheon 400-711South Korea Department of Internal Medicine Inha University School of MedicineIncheon 400-711South Korea

Peliosis hepatis is a rare pathological entity and may cause fatal hepatic hemorrhage and liver ***, we present a young male patient with aplastic anemia,who had received long-term treatment with *** patient suffered from sudden onset of intra-abdominal hemorrhage with profuse *** patient was treated successfully with a right hemihepatectomy and is in good health after 13 postoperative *** suggest that peliosis hepatis be considered in patients with hepatic parenchymal hematoma,especially in patients under prolonged synthetic anabolic steroid *** possibility of a potentially life-threatening complication of massive intra-abdominal bleeding should also be considered.

关键词： Peliosis hepatis Spontaneous rupture Liver

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Interstitial tissue-specific gene expression in mouse testis by intra-tunica albuguineal injection of recombinant baculovirus

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Asian Journal of Andrology 2009年第3期11卷 342-350页

作者： Hyun Jung Park Won Young lee Jin Hoi Kim Jae Hwan Kim Hun Jong Jung Nam hyung Kim Bo Kyung Kim Hyuk Song Department of Animal Science College of Natural Science Konkuk University Chung-ju 380-701 Korea CHA Stem Cell Institute Graduate School of Life Science and Biotechnology Pochon CHA University Seoul 135-907 Korea Department of Animal Biotechnology College of Animal Biotechnology Konkuk University Seoul 143-701 Korea Occupation and Environment Department Medical School Konkuk University Chung-ju 380-704 Korea Department of Animal Science College of Agriculture Chungbuk National University Choung-ju Chung-buk 361-763 Korea Department of Physiology Medical School Konkuk University Chung-ju 380-701 Korea

The purpose of this study is to establish a gene delivery system for interstitial tissue-specific protein expression in mice testes using modified recombinant baculovirus. Green fluorescent protein （GFP）-expressing recombinant baculovirus （GFP-baculovirus）, in which the insect cell-specific polyhedron promoter was replaced by the cytomegalovirus （CMV）-IE promoter, was used to transfect testicular cells in vitro, and for intra-tunica albuguineal injection of the interstitial tissue of the testis. GFP expression was monitored in frozen testes sections by fluorescence microscopy. Expression of GFP in testicular tissues was also assessed by reverse transcription polymerase chain reaction （RT-PCR）, and protein expression was assessed by Western blot. Testicular cells in vitro were infected efficiently by modified recombinant GFP-baculovirus. lntra-tunica albuguineal injection of GFP- baculovirus into the mouse testis resulted in a high level of GFP expression in the interstitial tissues. RT-PCR analysis clearly showed GFP gene expression in the testis, particularly interstitial tissues. Intra-tunica albuguineal injection of a modified baculovirus that encoded recombinant rat insulin-like growth factor binding protein （IGFBP）-5 resulted in an increase in IGFBP-5 in testis and semen. In conclusion, we have developed an efficient delivery system for gene expression in vivo in testicular cells, particularly cells of the interstitial tissue using intratunica albuguineal injection of a modified recombinant baculovirus. This method will be particularly relevant for application that requires gene delivery and protein expression in the testicular cells of the outer seminiferous tubule of the testis.

关键词： intra-tunica albuguineal injection recombinant baculovirus testis gene delivery

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