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Effects of Ginkgo biloba extracts with mirodenafil on the relaxation of corpus cavernosal smooth muscle and the potassium channel activity of corporal smooth muscle cells

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Asian Journal of Andrology 2011年第5期13卷 742-746页

作者： jung jun Kim Deok hyun Han Soo hyun Lim Tae Hun Kim Mee Ree Chae Kyung Jin Chung Sung Chul Kam ju-Hong Jeon Jong Kwan Parks Sung Won Lee Department of Urology Samsung Medical Center Sungkyunkwan University School of Medicine Seou1135-710 Korea Department of Urology Gachon University Gil Hospital Incheon 405-760 Korea Department of Urology Gyeengsang National University College of Medicine Gyeongsangnam-do 660-702 Korea Department of Physiology and Biophysics Seeul National University College of Medicine Seeul 110-799 Korea Department of Urology Medical School Chonbuk National University and Institute for Medical Sciences Chonbuk National University and Research Institute and Clinical Trial Center of Medical Device of Chonbuk National University Hospital Jeonju-si 561-712 Korea

In this study, we investigated the effects of a combination of Ginkgo biloba extracts （GBE） and phosphodiesterase type 5 （PDE-5） inhibitors on the muscular tone of the corpus cavernosum and potassium channel activity of corporal smooth muscle cells. Strips of corpus cavernosum from male New Zealand white rabbits were mounted in organ baths for isometric tension studies. After contraction with 1 × 10^-5 mol I^-1 norepinephrine, GBE （0.01-1 mg ml^-1） and mirodenafil （0.01-100 nmol I^-1） were added together into the organ bath. In electrophysiological studies, whole-cell currents were recorded by the conventional patch-clamp technique in cultured smooth muscle cells of the human corpus cavernosum. The corpus cavemosum was relaxed in response to GBE in a dose-dependent manner （from 0.64%±8.35% at 0.01 mg ml^-1 to 52.28%±11.42% at 1 mg ml^-1）. After pre-treatment with 0.03 mg ml^-1 of GBE, the relaxant effects of mirodenafil were increased at all concentrations, After tetraethylammonium （TEA）（1 mmol I^-1） administration, the increased effects were inhibited （P〈0.01）. Extracellular administration of GBE increased the whole-cell K^＋ outward currents in a dose-dependent fashion. The increase of the outward current was inhibited by I mmol 1-1 TEA. These results suggest that GBE could increase the relaxant potency of mirodenafil even at a minimally effective dose. The K＋ flow through potassium channels might be one of the mechanisms involved in this synergistic relaxation.

关键词： calcium-activated potassium channels erectile dysfunction Ginkgo biloba phosphodiesterase inhibitors smooth muscles

WNT5A drives interleukin-6-dependent epithelial-mesenchymal transition via the JAK/STAT pathway in keloid pathogenesis

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Burns & Trauma 2022年第1期10卷 200-215页

作者： Young In Lee jung Eun Shim Jihee Kim Won Jai Lee Jae Woo Kim Kee hyun Nam ju Hee Lee Department of Dermatology Severance HospitalCutaneous Biology Research InstituteYonsei University College of MedicineSeoul 03722Korea Bioinformatics Collaboration Unit Yonsei University College of MedicineSeoul 03722Korea Department of Plastic and Reconstructive Surgery Severance HospitalYonsei University College of MedicineSeoul 03722Korea Scar Laser and Plastic Surgery Center Yonsei Cancer HospitalYonsei University College of MedicineSeoul 03722Korea Department of Biochemistry and Molecular Biology Chronic Intractable Disease for Systems Medicine Research CenterYonsei University College of MedicineSeoul 03722Korea Department of Surgery Severance HospitalYonsei University College of MedicineSeoulKorea

Background:Keloid scarring is a fibroproliferative disease caused by aberrant genetic activation with an unclear underlying *** predisposition,aberrant cellular responses to environmental factors,increased inflammatory cytokines and epithelial-mesenchymal transition(EMT)phenomena are known as major *** this study,we aimed to identify the molecular drivers that initiate keloid ***:Bulk tissue RNA sequencing analyses of keloid and normal tissues along with ex vivo and in vitro tests were performed to identify the contributing genes to keloid *** animal model of inflammatory keloid scarring was reproduced by replication of a skin fibrosis model with intradermal bleomycin injection in C57BL/6 ***:Gene set enrichment analysis revealed upregulation of Wnt family member 5A(WNT5A)expression and genes associated with EMT in keloid ***,human keloid tissues and the bleomycin-induced skin fibrosis animal model showed significantly increased expression ofWNT5A and EMT *** activation of the interleukin(IL)-6/Janus kinase(JAK)/signal transducer and activator of transcription(STAT)pathway and subsequent elevation of EMT markerswas also observed in keratinocytes co-cultured withWNT5A-activated fibroblasts or keloid ***,WNT5A silencing and the blockage of IL-6 secretion via neutralizing IL-6 antibody reversed hyperactivation of the STAT pathway and EMT markers in ***,STAT3 silencing significantly reduced the EMT-like phenotypes in both keratinocytes and IL-6-stimulated ***:Intercellular communication via the WNT5A and STAT pathways possibly underlies a partial mechanism of EMT-like phenomena in keloid ***-6 secreted from WNT5A-activated fibroblasts or keloid fibroblasts activates the JAK/STAT signaling pathway in adjacent keratinocytes which in turn express EMT markers.A better understanding of keloid development and the

关键词： Keloid Wnt family member 5A Interleukin-6 JAK/STAT pathway Epithelial-mesenchymal transition Scar Epithelial mesenchymal transition

Identification of novel HLA-A 0201-restricted epitopes from anterior gradient-2 as a tumor-associated antigen against colorectal cancer

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Cellular & Molecular Immunology 2012年第2期9卷 175-183页

作者： hyun ju Lee Cheol Yi Hong Chun-Ji Jin Mi-hyun Kim Youn-Kyung Lee Thanh-Nhan Nguyen-Pham hyunah Lee Byoung Chul Park Ik-Joo Chung Hyeoung-Joon Kim Je-jung Lee Research Center for Cancer Immunotherapy Chonnam National University Hwasun Hospital Hwasun Jeollanamdo Korea Department of Hematology-Oncology Chonnam National University Gwangju Korea VaxcelI-Bio Therapeutics Hwasun Jeollanamdo Korea Samsung Medical Center Sungkyunkwan University School of Medicine Seoul Korea Korea Research Institute of Bioscience and Biotechnology （KRIBB） Daejeon Korea The Brain Korea 21 Project Center for Biomedical Human Resources at Chonnam National University Gwangiu Korea

Anterior gradient-2 （AGR2） promotes tumor growth, cell migration and cellular transformation and its enhanced expression is almost completely restricted to malignant tissues, thus making AGR2 an interesting target for the development of immunotherapeutic strategies. We investigated whether the AGR2 molecule comprises human leukocyte antigen （HLA）-A 0201-binding epitopes recognized by human cytotoxic T lymphocytes （CTLs）, which could be targeted in dendritic cell （DC）-based cancer immunotherapy against colorectal cancer （CRC）. We reviewed the sequence of AGR2 for peptides that could potentially bind to HLA-A 0201 with the aid of a computer-based program. Five candidate peptides with different binding scores were synthesized and tested. These peptides were then assessed for their immunogenicity to elicit specific immune responses mediated by CTLs in vitro by means of enzyme-linked immunospot assays and CTL assays. AGR2 was highly expressed in several CRC cell lines, including DK01, DLD1, KM 12C, HCT-8 and HT-29. DCs pulsed with AGR2-P2 （aa 11-19; LLVALSYTL） or AGR2-P4 （aa 127-135; RIMFVDPSL） generated potent CTLs that could lyse T2 cells pulsed with AGR2-P2 or AGR2-P4 and HLA-A0201＋ AGR2-positive CRC cell lines in a strong dose-dependent and HLA-A 0201-restricted manner. In conclusion, these novel epitopes derived from AGR2 protein may be attractive candidates for DC-based immunotherapy for CRC.

关键词： AGR2 colorectal cancer dendritic cell tumor-associated antigen

Optimal Growth Conditions of Microorganisms Isolated from the Buyeo Royal Tomb No. 1 and Their Effects on Painting Layers

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Advances in Microbiology 2022年第9期12卷 525-540页

作者： hyun ju Lee Yong Jae Chung Department of Heritage Conservation and Restoration Graduate School of Cultural Heritage Korea National University of Cultural Heritage Buyeo Republic of Korea

The Buyeo Royal Tomb No. 1 is an ancient tomb built in the late 6th and early 7th century. The four walls of the main room have murals of four guardian deities, and the ceiling has murals of lotus and cloud patterns. This study assessed the optimal growth conditions of two fungal (Fusarium oxysporum, Mortierella sp.) and four bacterial (Bacillus cereus, Cupriavidus campinensis, Streptomyces avidinii, Streptomyces cirratus) strains isolated from the Tomb No. 1, along with their effects on the painting layer. The two fungi showed optimal growth at 20°C - 30°C under both nutrient and non-nutrient conditions. These strains did not decompose or discolor the three pigments (cinnabar, hematite, oyster shell white);however, M. sp. showed slight decomposition of the media (starch paste, sea weed). The four bacterial strains showed the most active growth at 20°C - 25°C under nutrient conditions and did not grow under non-nutrient conditions. These bacteria commonly degraded animal glue and sea weed components. In addition, S. cirratus degraded starch. The genus Streptomyces discolored the pigment medium to brown and black, suggesting a possible risk of discoloration of the murals. The current environment in Tomb No. 1 was sufficient for microorganism growth, and the presence of strains such as soil bacteria and actinomycetes on the mural surface may damage the murals. The findings of this study could be helpful for preserving mural tombs against biological damage caused by microorganisms that are already present or may be present in the tombs in the future. These findings also provide guidelines for comprehensive conservation management.

关键词： Ancient Mural Tomb Murals Biodeterioration Buyeo Royal Tombs Growth Condition Painting Layer

Reinfection rate and endoscopic changes after successful eradication of Helicobacter pylori

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World Journal of Gastroenterology 2010年第2期16卷 251-255页

作者： Kum Hei Ryu Sun Young Yi Youn ju Na Su jung Baik Su Jin Yoon Hae-Sun jung hyun Joo Song Center for Cancer Prevention and Detection National Cancer CenterMadu1-dongIlsandong-guGoyangsiGyeonggi-do410-769South Korea Departments of Internal Medicine Ewha Womans University School of Medicine911-1 Mokdong Yancheon-guSeoul158-710South Korea

AIM:To determine the long-term outcomes regarding reinfection with Helicobacter pylori(***)and endoscopic changes after successful *** eradication. METHODS:From june 1994 to January 2007,186 patients(M:F=98:88;mean age 50.0±11.4 years), in whom *** had been successfully eradicated, were *** mean duration of follow up was 41.2±24.0 mo. RESULTS:*** reinfection occurred in 58 patients (31.2%).The average annual reinfection rate was 9.1% per patient *** recurrence of peptic ulcer was detected at the follow up *** were no significant differences between the *** eradication regimens for the reinfection rate and no significant differences in endoscopic findings between the *** group and the ***-cured ***:The reinfection rate in Korea is 9.1% which represents a decreasing *** was no relationship between *** infection status and changes in endoscopic *** was also no recurrence or aggravation of ulcers.

关键词： Helicobacter pylori Eradication Reinfection Endoscopy

Recent progress on the seasonal tropical cyclone predictions over the western North Pacific from 2014 to 2020

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Tropical Cyclone Research and Review 2022年第1期11卷 26-35页

作者： Eun-Jeong Cha Se Hwan Yang Yu Sun hyun Chang-Hoi Ho Il-ju Moon Meso-scale Meteorological Phenomenon Research Team National Institute of Meteorological SciencesKorean Meteorological AdministrationJejuRepublic of Korea National Typhoon Center Korean Meteorological AdministrationJejuRepublic of Korea School of Earth and Environmental Sciences Seoul National UniversitySeoulRepublic of Korea Typhoon Research Center Jeju National UniversityJejuRepublic of Korea

This study summarized the procedure for the seasonal predictions of tropical cyclones(TCs)over the western North Pacific(WNP),which is currently operating at the Korea Meteorological Administration(KMA),Republic of *** methodology was briefly described,and its prediction accuracy was *** predictions were produced by synthesizing spatiotemporal evolutions of various climate factors such as El Ni no–Southern Oscillation(ENSO),monsoon activity,and Madden–julian Oscillation(MJO),using four models:a statistical,a dynamical,and two statistical–dynamical *** KMA forecaster predicted the number of TCs over the WNP based on the results of the four models and season to season climate *** seasonal prediction of TCs is announced through the press twice a year,for the summer on May and fall on *** present results showed low accuracy during the period 2014–*** advance forecast skill,a set of recommendations are suggested.

关键词： Tropical cyclones Seasonal prediction Western north pacific Statistical model Statistical-dynamical model Dynamical model Forecast error and verification

Inhibition of advanced glycation endproducts formation by Korean thistle,Cirsium maackii

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Asian Pacific Journal of Tropical Medicine 2015年第1期8卷 1-5页

作者： hyun Ah jung Jin ju Park Byung Sun Min Hee Jin jung Md.Nurul Islam Jae Sue Choi Department of Food Science and Human Nutrition Chonbuk National University Department of Food and Life Science Pukyong National University College of Pharmacy Catholic University of Daegu

Objective:To evaluate inhibitoy potential of seven Korean thistles against the advanced glyeatinn endproducts(AGE) formation as well as to identify responsible compounds from the most active ***:We used an in vitro AGE inhibition assay to evaluate the antidiabetic complication potential of the methanol extracts of the selected Korean ***:Among the seven Korean thistles,the leaves of Cirsium maackii(***) exhibited the most significant inhibitory activity against AGE *** means of bioassay-directed fractionation,a *** acid and 14 flavonoids were isolated from the active ethyl acetate soluble fraction of a methanol extract from *** *** and its 5-O-glueoside have been previously isolated:however,a lignan and 13 known compounds were isolated for the first lime from C maackii leaves in this *** of the isolated compounds exhibited inhibitory activities against potential AGE *** them,cernuoside was shown to be the most potent AGE inhibitor with an IC_(50) value of 21.21 μ mol/*** importantly,two major flavonoids,luteolin and ils 5-O-glucoside,also significantly inhibited AGE formation,with IC_(50) values of36.33 and 37.47 μmol/L,*** activity relationship revealed that the presence of free 3'and 4' dihydroxyl group in flavonoids skeleton played an important role in AGE ***:These results indicate that *** and C maackii-derived flavonoids might be explored further to develop therapeutic agents for the prevention of diabetic eoniplicalions due to their significant inhibitory activity against AGE formation.

关键词： Flavonoids Thistle Advanced glycation endproducts Diabetic complications Cirsium maackii

Suppressed estrogen supply via extra-ovarian progesterone receptor membrane component 1 in menopause

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The Journal of Biomedical Research 2021年第3期35卷 228-237页

作者： Sang RLee hyun Yang Seong Lae Jo Young Ho Lee Hye Won Lee Bae-keun Park Eui-ju Hong College of Veterinary Medicine Chungnam National UniversityDaejeon 34134Republic of Korea KM Convergence Research Division Korea Institute of Oriental MedicineDaejeon 34054Republic of Korea

In post-menopausal women,intra-mammary estrogen,which is converted from extra-ovarian estrone(E1),promotes the growth of breast *** the aromatase inhibitor letrozole does not suppress 17β-estradiol(E2)production from E1,high intra-mammary E1 concentrations impair letrozole's therapeutic *** receptor membrane component 1(Pgrmc1)is a non-classical progesterone receptor associated with breast cancer *** the present study,we introduced a Pgrmc1 heterozygous knockout(hetero KO)murine model exhibiting low Pgrmc1 expression,and observed estrogen levels and steroidogenic gene ***ïve Pgrmc1 hetero KO mice exhibited low estrogen(E2 and E1)levels and low progesterone receptor(PR)expression,compared to wild-type *** contrast,Pgrmc1 hetero KO mice that have been ovariectomized(OVX),including letrozole-treated OVX mice(OVX-letrozole),exhibited high estrogen levels and PR *** extra-ovarian estrogen production in Pgrmc1 hetero KO mice was observed with the induction of steroid sulfatase(STS).In MCF-7 cell,letrozole suppressed PR expression,but PGRMC1 knockdown increased PR and STS *** presented results highlight the important role of Pgrmc1 in modulating estrogen production when ovary-derived estrogen is limited,thereby suggesting a potential therapeutic approach for letrozole resistance.

关键词： extra-ovarian Pgrmc1 steroid sulfatase estrogen letrozole

Contemporary trends in radical prostatectomy and predictors of recovery of urinary continence in men aged over 70 years:comparisons between cohorts aged over 70 and less than 70 years

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Asian Journal of Andrology 2020年第3期22卷 280-286页

作者： Young ju Lee Jin-Woo jung Sangchul Lee Sang Wook Lee Jeong hyun Kim Sung Kyu Hong Seok-Soo Byun Sang Eun Lee Seong Jin Jeong Departme nt of Urology Seoul National University Bundang HospitalSeongnam 13620Korea Department of Urology Catholic Kwan dong Univerisity International St.Mary's HospitalIncheon 22711Korea Department of Urology Kangwon National University School of MedicineChuncheon 24341Korea.

We evaluated contemporary trends in radical prostatectomy(RP)in men aged>70 years and investigated associations of selected variables with recovery of urinary continence(UC)in two age groups:>70 andohort of 2301 eligible patients attending our institution from 2004 to 2015 was *** were divided into two groups based on age at surgery(>70 years[n=610]vs70 years gradually increased up to 30.0%,and the rate of robot-assisted RP and neurovascular bundle(NVB)saving increased continually to 80.0%and 67.4%of older patients,*** the rate of recovery of UC within 12 months(3 months)in patients aged>70 years was lower than that in those aged S70 years(81.5%[52.6%]vs 88.6%[60.9%],respectively;both P70 ***,unlike younger patients,only variables(age and MUL),possibly associated with the inhere nt fun ction of the urinary sphi ncter,were predictors of recovery of UC in patients aged>70 years.

关键词： elderly predictor prostate cancer radical prostatectomy urinary incontinence