检索结果-南通市图书馆

Anti-cancer effect of ethylacetate fraction from Orostachys japonicus on HT-29 human colon cancer cells by induction of apoptosis through caspase-dependent signaling pathway

引用

Asian Pacific Journal of Tropical Medicine 2018年第5期11卷 330-335页

作者： Deok-Seon Ryu Hyun-ji Lee ji-hye Kwon Dong-Seok Lee Department of Biomedical Laboratory Science Soonchunhyang University Department of Smart Foods and Drugs Graduate School of Inje University

Objective: To investigate the anti-colon cancer effects of ethylacetate fraction from Orostachys japonicus(0. japonicus) on HT-29 cancer cells. Methods: The viability of HT-29 cells was assayed by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2 H-tetrazolium(MTS) method. Apoptosis induction and cell cycle inhibition were confirmed by fluorescein isothiocyanate and propidium iodide staining using flow *** changes in the nucleus were observed, using a fluorescence microscope with4',6-diamidino-2-phenylindole(DAPI) nuclear staining. The expression levels of the upstream and downstream proteins involved in the anti-cancer mechanism were confirmed by Western blotting. Results: After treating HT-29 cells with different concentrations of ethylacetate fraction from O. japonicus, the viability of cells decreased in a concentration-dependent manner,while apoptosis induction and apoptotic body formation increased. Cell cycle analysis showed that the arrest occurred at the sub-G_1 and S phase. Among the upstream and downstream proteins involved in anti-cancer activity, the level of B cell lymphoma-2 decreased, and the bcl-2-associated x protein increased. The level of pro-caspase-3, pro-caspase-8, and pro-caspase-9 decreased, while the level of cleaved-caspase-3, cleaved-caspase-8, and cleaved-caspase-9 increased. Moreover, the phosphorylation, that is, activation of extracellular signal regulated kinase 1/2, Jun-N-terminal kinase, and p38 increased. Conclusions: Combining the above results, it is thought that the survival of HT-29 cells is suppressed by ethylacetate fraction from0. japonicus through mitochondrial regulation-induced caspase cascade activation, induction of apoptosis and cell cycle arrest.

关键词： Orostachys japonicus HT-29 human colon cancer cells Anti-cancer activity Apoptosis Caspase cascade

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Targeted and effective photodynamic therapy for cancer using functionalized nanomaterials

引用

Acta Pharmaceutica Sinica B 2016年第4期6卷 297-307页

作者： eun ji Hong Dae Gun Choi Min Suk Shim Division of Bioengineering Incheon National University

Photodynamic therapy(PDT) is an emerging, non-invasive therapeutic strategy that involves photosensitizer(PS) drugs and external light for the treatment of diseases. Despite the great progress in PS-mediated PDT, their clinical applications are still hampered by poor water solubility and tissue/cell specificity of conventional PS drugs. Therefore, great efforts have been made towards the development of nanomaterials that can tackle fundamental challenges in conventional PS drug–mediated PDT for cancer treatment. This review highlights recent advances in the development of nano-platforms, in which various functionalized organic and inorganic nanomaterials are integrated with PS drugs, for significantly enhanced efficacy and tumor-selectivity of PDT.

关键词： Organic nanomaterials Inorganic nanomaterials Nanoparticles Photodynamic therapy Photosensitizer Targeted therapy Cancer therapy Near-infrared light

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Characteristics of Sea Breeze Front Development with Various Synoptic Conditions and Its Impact on Lower Troposphere Ozone Formation

引用

Advances in Atmospheric Sciences 2013年第5期30卷 1461-1478页

作者： Hyo-eun ji Soon-Hwan LEE Hwa-Woon LEE Division of Earth Environmental System Pusan National University Department of Earth Science Education Pusan National University

To examine the correlation between the sizes of sea breeze fronts and pollutants under the influence of synoptic fields, a numerical simulation was conducted in the southeast coastal area of the Korean Peninsula, where relatively high concentrations of pollutants occur because of the presence of various kinds of industrial developments. Sea breeze and sea breeze front days during the period 2005 09 were identified using wind profiler data and, according to the results, the number of days were 72 and 53, respectively. When synoptic forcing was weak, sea breeze fronts moved fast both in horizontal fields and in terms of wind velocity, while in the case of strong synoptic forcing, sea breeze fronts remained at the coast or moved slowly due to strong opposing flows. In this case, the sea breeze front development function and horizontal potential temperature difference were larger than with weak synoptic forcing. The ozone concentration that moves together with sea breeze fronts was also formed along the frontal surfaces. Ozone advection and diffusion in the case of strong synoptic forcing was suppressed at the frontal surface and the concentration gradient was large. The vertical distribution of ozone was very low due to the Thermal Internal Boundary Layer （TIBL） being low.

关键词： sea breeze front synoptic opposing flow wind profiler ozone numerical simulation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

CRISPR/Cas9-mediated gene editing to confer turnip mosaic virus (TuMV) resistance in Chinese cabbage (Brassica rapa)

引用

Horticulture Research 2023年第6期10卷 135-143页

作者： Ye-Rin Lee Muhammad Irfan Siddique Do-Sun Kim eun Su Lee Koeun Han Sang-Gyu Kim hye-eun Lee Vegetable Research Division National Institute of Horticultural and Herbal ScienceRural Development AdministrationWanju55365Republic of Korea Department of Horticultural Sciences North Carolina State University Mountain Horticultural Crops ResearchExtension Center 455 Research DriveMills RiverNC 28759USA Department of Biological Sciences Korea Advanced Institute for Science and TechnologyDaejeon34141Republic of Korea

Genome editing approaches,particularly the CRISPR/Cas9 technology,are becoming state-of-the-art for trait development in numerous breeding *** advances in improving plant traits are enabled by this influential tool,especially for disease resistance,compared to traditional *** of the potyviruses,the turnip mosaic virus(TuMV),is the most widespread and damaging virus that infects Brassica *** generated the targeted mutation at the eIF(iso)4E gene in the TuMV-susceptible cultivar“Seoul”using CRISPR/Cas9 to develop TuMV-resistant Chinese *** detected several heritable indel mutations in the edited T0 plants and developed T1 through generational *** was indicated in the sequence analysis of the eIF(iso)4E-edited T1 plants that the mutations were transferred to succeeding *** edited T1 plants conferred resistance to *** was shown with ELISA analysis the lack of accumulation of viral ***,we found a strong negative correlation(r=-0.938)between TuMV resistance and the genome editing frequency of eIF(iso)***,it was revealed in this study that CRISPR/Cas9 technique can expedite the breeding process to improve traits in Chinese cabbage plants.

关键词： CRISPR/Cas9 TuMV breeding

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Significance of adverse outcome pathways in biomarker-based environmental risk assessment in aquatic organisms

引用

Journal of Environmental Sciences 2015年第9期27卷 115-127页

作者： jin Wuk Lee eun-ji Won Sheikh Raisuddin Jae-Seong Lee Department of Biological Science College of Science Sungkyunkwan University Department of Medical Elementology and Toxicology Hamdard University

In environmental risk assessments（ERA）, biomarkers have been widely used as an early warning signal of environmental contamination. However, biomarker responses have limitation due to its low relevance to adverse outcomes（e.g., fluctuations in community structure, decreases in population size, and other similar ecobiologically relevant indicators of community structure and function）. To mitigate these limitations, the concept of adverse outcome pathways（AOPs） was developed. An AOP is an analytical, sequentially progressive pathway that links a molecular initiating event（MIE） to an adverse outcome. Recently, AOPs have been recognized as a potential informational tool by which the implications of molecular biomarkers in ERA can be better understood. To demonstrate the utility of AOPs in biomarker-based ERA, here we discuss a series of three different biological repercussions caused by exposure to benzo（a）pyrene（Ba P）, silver nanoparticles（Ag NPs）, and selenium（Se）. Using mainly aquatic invertebrates and selected vertebrates as model species, we focus on the development of the AOP concept. Aquatic organisms are suitable bioindicator species whose entire lifespans can be observed over a short period; moreover, these species can be studied on the molecular and population ***, interspecific differences between aquatic organisms are important to consider in an AOP framework, since these differences are an integral part of the natural *** development of an environmental pollutant-mediated AOP may enable a better understanding of the effects of environmental pollutants in different scenarios in the diverse community of an ecosystem.

关键词： Adverse outcome pathway Biomarker Omics Environmental risk assessment Aquatic organisms Interspecific difference

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Design of 1 kbit antifuse one time programmable memory IP using dual program voltage

引用

Journal of Central South University 2011年第1期18卷 125-132页

作者：金丽妍 JANG ji-hye KIM Du-Hwi KIM Young-Hee Department of Electronic Engineering Changwon National University9 Sarim-DongChangwon 641-773Korea

A 1 kbit antifuse one time programmable(OTP) memory IP,which is one of the non-volatile memory IPs,was designed and used for power management integrated circuits(ICs).A conventional antifuse OTP cell using a single positive program voltage(VPP) has a problem when applying a higher voltage than the breakdown voltage of the thin gate oxides and at the same time,securing the reliability of medium voltage(VM) devices that are thick gate transistors.A new antifuse OTP cell using a dual program voltage was proposed to prevent the possibility for failures in a qualification test or the yield *** the newly proposed cell,a stable sensing is secured from the post-program resistances of several ten thousand ohms or below due to the voltage higher than the hard breakdown voltage applied to the terminals of the *** layout size of the designed 1 kbit antifuse OTP memory IP with Dongbu HiTek's 0.18 μm Bipolar-CMOS-DMOS(BCD) process is 567.9 μm×205.135 μm and the post-program resistance of an antifuse is predicted to be several ten thousand ohms.

关键词： one time programmable memory IP antifuse hard breakdown dual program voltage post-program resistance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

SARS-CoV-2 mutations, vaccines, and immunity: implication of variants of concern

引用

Signal Transduction and Targeted Therapy 2021年第6期6卷 1638-1639页

作者： ji Yun Noh hye Won Jeong Eui-Cheol Shin Division of Infectious Diseases Department of Internal MedicineKorea University Guro HospitalKorea University College of MedicineSeoulRepublic of Korea Graduate School of Medical Science and Engineering Korea Advanced Institute of Science and Technology(KAIST)DaejeonRepublic of Korea Division of Infectious Diseases Department of Internal MedicineChungbuk National University College of MedicineCheongjuRepublic of Korea The Center for Epidemic Preparedness KAISTDaejeonRepublic of Korea

In a recent study published in Nature,Wang et al.1 investigated the neutralizing activities of antibodies elicited by COVID-19 mRNA vaccines and natural infection with SARS-CoV-2 against SARS-CoV-2 *** devastating impact of the ongoing COVID-19 pandemic on public health,the economy,and society has made vaccine development a top priority for global ***,vaccine development is progressing at an unprecedented pace as an urgent response to COVID-19.

关键词： immunity vaccines variants

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Time-dependent effect of combination therapy with erythropoietin and granulocyte colony-stimulating factor in a mouse model of hypoxic-ischemic brain injury

引用

Neuroscience Bulletin 2014年第1期30卷 107-117页

作者： ji Hea Yu Jung Hwa Seo Jong eun Lee ji Hoe Heo Sung-Rae Cho Department and Research Institute of Rehabilitation Medicine Yonsei University College of Medicine Brain Korea 21 PLUS Project for Medical Science Yonsei University Graduate Program of Nano Science and Technology Yonsei University Department of Anatomy Yonsei University College of Medicine Department of Neurology Yonsei University College of Medicine Yonsei Stem Cell Center Avison Biomedical Research CenterYonsei University College of Medicine Rehabilitation Institute of Neuromuscular Disease Yonsei University College of Medicine

Erythropoietin （EPO） and granulocyte colony- stimulating factor （G-CSF） are likely to play broad roles in the brain. We investigated the effects of combination therapy with EPO and G-CSF in hypoxic- ischemic brain injury during the acute, subacute, and chronic phases. A total of 79 C57BL/6 mice with hypoxic-ischemic brain injury were randomly assigned acute （days 1-5）, subacute （days 11-15）and chronic （days 28-32） groups. All of them were treated with G-CSF （250 μg/kg） and EPO （5 000 U/kg） or saline daily for 5 consecutive days. Behavioral assessments and immunohistochemistry for angiogenesis, neuro- genesis, and astrogliosis were performed with an 8-week follow-up. Hypoxia-inducible factor-1 （HIF-1） was also measured by Western blot analysis. The results showed that the combination therapy with EPO and G-CSF in the acute phase significantly improved rotarod performance and forelimb-use symmetry compared to the other groups, while subacute EPO and G-CSF therapy exhibited a modest improvement compared with the chronic saline controls. The acute treatment significantly increased the density of CD31^＋（PECAM-1） and a-smooth muscle actin^＋ vessels in the frontal cortex and striatum, increased BrdU^＋/PSA- NCAM^＋ neurogenesis in the subventricular zone, and decreased astroglial density in the striatum. Furthermore, acute treatment significantly increased the HIF-1 expression in the cytosol and nucleus, whereas chronic treatment did not change the HIF-1 expression, consistent with the behavioral outcomes. These results indicate that the induction of HIF-1 expression by combination therapy with EPO and G-CSF synergistically enhances not only behavioral function but also neurogenesis and angiogenesis while decreasing the astroglial response in a time- dependent manner.

关键词： erythropoietin granulocyte colony-stimulating factor hypoxia-inducible factor-1 hypoxic-ischemic brain injury

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Experience of primary intestinal lymphangiectasia in adults: Twelve case series from a tertiary referral hospital

引用

World Journal of Clinical Cases 2024年第4期12卷 746-757页

作者： ji eun Na ji eun Kim Sujin Park eun Ran Kim Sung Noh Hong Young-Ho Kim Dong Kyung Chang Department of Internal Medicine Inje University Haeundae Paik HospitalBusan 48108South Korea Department of Medicine Samsung Medical CenterSeoul 06351South Korea Department of Pathology Samsung Medical CenterSeoul 06351South Korea

BACKGROUND While primary intestinal lymphangiectasia(PIL)is considered a rare condition,there have been several reported cases in ***,the absence of clear guidance from diagnosis to treatment and prognosis poses challenges for both physicians and *** To enhance understanding by investigating clinical presentation,diagnosis,treatment,complications,and prognoses in adult PIL *** We enrolled adult patients diagnosed with PIL between March 2016 and September *** primary outcome involved examining the diagnosis and treatment process of these *** secondary outcomes included identifying complications(infections,thromboembolism)and assessing prognoses(frequency of hospitalization and mortality)during the follow-up *** Among the 12 included patients,peripheral edema(100%)and diarrhea(75%)were the main presenting *** tests showed that all the pati-ents exhibited symptoms of hypoalbuminemia and ***,the predominant findings were edema of the small intestine(67%)and ascites(58%).The typical endoscopic finding with a snowflake appearance was observed in 75%of *** the 12 patients,two responded positive-ly to octreotide and sirolimus,and eight who could undergo maintenance therapy discontinued *** due to PIL led to infection in half of the patients,thromboembolism in three patients,and one *** PIL can be diagnosed in adults across various age groups,with different severity and treatment responses among patients,leading to diverse complications and ***,tailored treatments will be *** anticipate that our findings will contribute to the management of PIL,an etiology of protein-losing enteropathy.

关键词： Primary intestinal lymphangiectasia in adults Protein-losing enteropathy Diagnosis Treatment Prognosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Gyejibokryeong-Hwan improves recovery of injured muscles in mouse model of muscle contusion

引用

Journal of Traditional Chinese Medicine 2021年第3期41卷 406-413页

作者： Chi Ho Lee Jeong June Choi Joo hye Sim Younmin Shin Seung-Joon Oh ji-Hyang Gu Hyun Ju Ha Dong Hwi Jeon eun-Jung Lee Min-Seok Oh Department of Korean Medicine Rehabilitation College of Korean MedicineDaejeon UniversityDaejeon 34520Republic of Korea Laboratory of Molecular Medicine College of Korean MedicineDaejeon UniversityDaejeon 34520Republic of Korea

OBJECTIVE: To investigate the effects of Gyejibokryeong-Hwan(Guizhifuling-wan, GBH) on muscle injury in a mouse model of muscle ***: C57/BL6 mouse biceps femoris muscles were injured using the drop-mass method and injured animals were treated orally with GBH(50,100, or 500 mg/kg) once a day for 7 d. Open field and treadmill running tests were performed to assess functional recovery from muscle injury. The production of pro-inflammatory cytokines was examined by enzyme-linked immunosorbent assay and Western blotting analysis. Expression of the muscle regeneration biomarkers, myoblast determination(Myo D), myogenic factor 5(Myf5), and smooth muscle actin(α-SMA), in the biceps femoris muscle was investigated at the protein and m RNA level by Western blotting and real time-PCR, respectively. Histological analysis was performed using hematoxylin and eosin staining. Finally, myosin heavy chain production was investigated in differentiated C2C12 myoblasts in the presence of ***: GBH treatment markedly improved locomotion and running behavior. GBH significantly inhibited the secretion of monocyte chemoattractant protein-1 into the bloodstream in muscle-contused animals. The levels of Myo D, Myf5, and α-SMA protein and m RNA were significantly up-regulated by GBH in injured muscle tissue. Histological studies suggested that GBH facilitated recovery from muscle damage. However, GBH did not induce the production of myosin heavy chain in ***: Overall, the present study suggested that GBH improves the recovery of the injured muscles in the mouse model of muscle contusion.

关键词： muscles wounds and injuries contusions regeneration muscle skeletal myogenic regulatory factor 5 Gyejibokryeong-Hwan

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：