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Akt Gene Therapy Enhances Angiogenesis in Ischemic Hind Limb

Chemical Research in Chinese Universities 2010年第3期26卷 409-414页

作者： huo xin zhang qiang liu ying Department of Geriatrics First Affiliated Hospital China Medical University Shenyang 110001 P. R. China

Both bone marrow-derived mesenchymal stem cells（MSCs） therapy and gene therapy have been applied to animal studies and clinical trials. The goal of this study was to compare the effects of three types of MSCs transplantation. In the first part of the study, MSCs were transduced with the pEGFP-C1 and transfected with the pEGFP-C1/Akt in a model of bilateral hind limb ischemia in rats. Following gene transduction, the production of Akt and VEGF protein was more in the culture media of GFP-Akt/MSCs group than that in those of the GFP/MSCs group In the second part of the study, Sprague-Dawley rats（n=10/group） underwent surgery to create bilateral hind limb ischemia and were randomized into two groups consisting of a GFP/Akt-MSCs group（MSCs suspension, 1×10^7 MSCs/100 μL transfected pEGFP-C1/Akt） and a GFP-MSCs group（MSCs suspension, 1 × 10^7 MSCs/100 μL）. These PEGFP-C1/Akt and PEGFP-C 1 transfected MSCs suspensions were slowly infused into the adductor muscles of the rat＇s left hind limb, while the rat＇s right hind limb in the control group received an equal volume of PBS. Endpoints includes angiographic analysis, evaluation of capillary density, immunohistochemistry for von Willebrand factor （vWF）, immunodetection of Akt and VEGF protein, RT-PCR of VEGF and Akt mRNA levels in vitro and in vivo. Our data indicate that the tissue perfusion can improve capillary density and the mature of vasculature in the GFP-Akt/MSCs group compared to that in the GFP-MSCs group or control group in the rat model of bilateral hind limb ischemia. Transplantation of MSCs transfected with Akt gene may become the future therapy for hind limb ischemia.

关键词： Bone mesenchymal stem cell pEGFP-C1/Akt Hindlimb Ischemia Angiogenesis

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BET Surface Area and Porous Structure of Fullerenes and Fullerene Soots

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Chemical Research in Chinese Universities 1999年第1期15卷 92-95页

作者： CHEN Jian-xin SHENG Guo-ying liu Shu-ying liu Zi-yang liu Zhi-qiang FU Jia-mo zhang Xiao-mei Changchun Institute of Applied Chemistry a Chinese Academy of SciencesChangchun130022 State Key Laboratory of Organic Geochemistry Guangzhou Institute of Geochemistry Chinese Academy of ScienoesGuangzhou510640 Institute of Animal Saience Guangdong Acadeny of Agricultural Science510640 Environmental Monitoring Centeral Station off Jilin Province Changchun130011

ＩｎｔｒｏｄｕｃｔｉｏｎＡｆａｓｃｉｎａｔｉｎｇｓｏｌｉｄｂｌａｃｋｃｏｎｄｅｎｓａｔｅ（ｈｅｒｅｃａｌｅｄｆｕｌｅｒｅｎｅｓｏｏｔ）ｉｓｇｅｎｅｒａｔｅｄｉｎｔｈｅｇａｓｐｈａｓｅａｎｄｄｅｐｏｓｉｔｅｄｄｕｒｉｎ... 详细信息

ＩｎｔｒｏｄｕｃｔｉｏｎＡｆａｓｃｉｎａｔｉｎｇｓｏｌｉｄｂｌａｃｋｃｏｎｄｅｎｓａｔｅ（ｈｅｒｅｃａｌｅｄｆｕｌｅｒｅｎｅｓｏｏｔ）ｉｓｇｅｎｅｒａｔｅｄｉｎｔｈｅｇａｓｐｈａｓｅａｎｄｄｅｐｏｓｉｔｅｄｄｕｒｉｎｇｔｈｅｄｉｓｃｈａｒｇｅｉｎａＫ...

关键词： Fullerenes Fullerene soots Surface area Pore size distribution Adsorbent

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A new seamoth species of Pegasus(Syngnathiformes:Pegasidae)from the East China Sea

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Zoological Research 2022年第4期43卷 675-678页

作者： ying-Yi zhang Rong-Rong zhang Shao-Bo Ma Shuai-Shuai liu qiang Lin xin Wang CAS Key Laboratory of Tropical Marine Bio-resources and Ecology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhouGuangdong 510301China Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) GuangzhouGuangdong 511458China State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology Xiamen UniversityXiamenFujian 361102China University of the Chinese Academy of Sciences Beijing 100049China Laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)QingdaoShandong 266237China

DEAR EDITOR,The Pegasidae family,which includes seven species of seamoths,is widely distributed in the temperate and tropical waters of the Indo-Pacific,ranging from South Africa to *** this study,we describe a new species of seamoth,Pegasus sinensis ***.,based on morphological and molecular characterization of 14 specimens collected from the surrounding waters of Xiamen City,Fujian Province,*** new species differs from closely related *** in both the density and color of spots present on the dorsal and lateral body surfaces.

关键词： Xiamen asi temperate

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Stellate ganglion block reduces inflammation and improves neurological function in diabetic rats during ischemic stroke

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Neural Regeneration Research 2022年第9期17卷 1991-1997页

作者： Ting-Ting Li qiang Wan xin zhang Yuan Xiao Li-ying Sun Yu-Rong zhang Xiang-Nan liu Wan-Chao Yang Department of Anesthesiology the 2nd Affiliated Hospital of Harbin Medical UniversityHarbinHeilongjiang ProvinceChina

Diabetes mellitus is an independent risk factor for ischemic *** diabetes mellitus and stroke are linked to systemic inflammation that aggravates patient *** ganglion block can effectively regulate the inflammatory ***,it is hypothesized that stellate ganglion block could be a potential therapy for ischemic stroke in diabetic *** this study,we induced diabetes mellitus in rats by feeding them a high-fat diet for 4 successive *** left middle cerebral artery was occluded to establish models of ischemic stroke in diabetic ***,we performed left stellate ganglion block with 1%lidocaine using the percutaneous posterior approach 15 minutes before reperfusion and again 20 and 44 hours after *** results showed that stellate ganglion block did not decrease the blood glucose level in diabetic rats with diabetes mellitus but did reduce the cerebral infarct volume and the cerebral water *** also improved the recovery of neurological function,increased 28-day survival rate,inhibited Toll like receptor 4/nuclear factor kappa B signaling pathway and reduced inflammatory response in the plasma of ***,injection of Toll like receptor 4 agonist lipopolysaccharide 5 minutes before stellate ganglion block inhibited the effect of stellate ganglion block,whereas injection of Toll like receptor 4 inhibitor TAK242 had no such *** also found that stellate ganglion block performed at night had no positive effect on diabetic ischemic *** findings suggest that stellate ganglion block is a potential therapy for diabetic ischemic stroke and that it may be mediated through the Toll like receptor 4/nuclear factor kappa B signaling *** also found that the therapeutic effect of stellate ganglion block is affected by circadian rhythm.

关键词： circadian rhythms diabetes mellitus inflammation ischemic stroke long-term prognosis neurological function neuron nuclear factor kappa B stellate ganglion block Toll like receptor 4

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Reduced He ion irradiation damage in ZrC-based high-entropy ceramics

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Journal of Advanced Ceramics 2023年第5期12卷 916-929页

作者： Xiao-Ting xin Weichao Bao xin-Gang Wang Xiao-Jie Guo ying Lu Chenxi Zhu Ji-Xuan liu qiang Li Fangfang Xu Guo-Jun zhang State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsShanghai 200050China bState Key Laboratory for Modification of Chemical Fibers and Polymer Materials Institute of Functional MaterialsDonghua UniversityShanghai 201620China University of Chinese Academy of Sciences Beijing 100049China School of Engineering Westlake UniversityHangzhou 310024China

Excellent irradiation resistance is the basic property of nuclear materials to keep nuclear *** high-entropy design has great potential to improve the irradiation resistance of the nuclear materials,which has been proven in ***,whether or not high entropy can also improve the irradiation resistance of ceramics,especially the mechanism therein still needs to be *** this work,the irradiation and helium(He)behaviors of zirconium carbide(ZrC)-based high-entropy ceramics(HECs),i.e.,(Zr_(0.2)Ti_(0.2)Nb_(0.2)Ta_(0.2)W_(0.2))C,were investigated and compared with those of ZrC under 540 keV He ion irradiation with a dose of 1×10^(17) cm^(−2) at room temperature and subsequent *** ZrC and(Zr_(0.2)Ti_(0.2)Nb_(0.2)Ta_(0.2)W_(0.2))C maintain lattice integrity after irradiation,while the irradiation-induced lattice expansion is smaller in(Zr_(0.2)Ti_(0.2)Nb_(0.2)Ta_(0.2)W_(0.2))C(0.78%)with highly thermodynamic stability than that in ZrC(0.91%).After annealing at 800℃,ZrC exhibits the residual _(0.2)0%lattice expansion,while(Zr_(0.2)Ti_(0.2)Nb_(0.2)Ta_(0.2)W_(0.2))C shows only 0.10%.Full recovery of the lattice parameter(a)is achieved for both ceramics after annealing at 1500℃.In addition,the high entropy in the meantime brings about the favorable structural evolution phenomena including smaller He bubbles that are evenly distributed without abnormal coarsening or aggregation,segregation,and shorter and sparser *** excellent irradiation resistance is related to the high-entropy-induced phase stability,sluggish diffusion of defects,and stress dispersion along with the production of vacancies by valence *** present study indicates a high potential of high-entropy carbides in irradiation resistance applications.

关键词： high-entropy carbides zirconium carbide(ZrC) irradiation damage dislocations helium(He)bubbles

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Human cytomegalovirus RNA2.7 inhibits RNA polymeraseⅡ(PolⅡ)Serine-2 phosphorylation by reducing the interaction between PolⅡand phosphorylated cyclin-dependent kinase 9(pCDK9)

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Virologica Sinica 2022年第3期37卷 358-369页

作者： Yujing Huang xin Guo Jing zhang Jianming Li Mingyi Xu Qing Wang Zhongyang liu Yanping Ma ying Qi qiang Ruan Virology Laboratory Shengjing Hospital of China Medical UniversityShenyang110004China Department of Pediatrics Shengjing Hospital of China Medical UniversityShenyang110004China Department of Obstetrics and Gynecology Shengjing Hospital of China Medical UniversityShenyang110004China Department of Pediatrics The Fourth Affiliated HospitalChina Medical UniversityShenyang110033China

Human cytomegalovirus(HCMV)is a ubiquitous pathogen belongs to betaherpesvirus ***2.7 is a highly conserved long non-coding RNA accounting for more than 20%of total viral *** our study,functions of HCMV RNA2.7 were investigated by comparison of host cellular transcriptomes between cells infected with HCMV clinical strain and RNA2.7 deleted *** was demonstrated that RNA polymeraseⅡ(PolⅡ)-dependent host gene transcriptions were significantly activated when RNA2.7 was removed during infection.A145 nt-in-length motif within RNA2.7 was identified to inhibit the phosphorylation of PolⅡSerine-2(PolⅡS2)by reducing the interaction between PolⅡand phosphorylated cyclin-dependent kinase 9(pCDK9).Due to the loss of PolⅡS2 phosphorylation,cellular DNA pre-replication complex(pre-RC)factors,including Cdt1 and Cdc6,were significantly decreased,which prevented more cells from entering into S phase and facilitated viral DNA *** results provide new insights of HCMV RNA2.7 functions in regulation of host cellular transcription.

关键词： Human cytomegalovirus(HCMV) RNA2.7 RNA polymeraseⅡ(PolⅡ) Cyclin-dependent kinase 9(CDK9) Phosphorylation

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Prenylated stilbenes and flavonoids from the leaves of Cajanus cajan

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Chinese Journal of Natural Medicines 2019年第5期17卷 381-386页

作者： WU Gui-Yun zhang Xiao GUO Xue-ying huo Lu-Qiong liu Hong-xin SHEN Xiao-Ling QIU Sheng-Xiang HU ying-Jie TAN Hai-Bo Laboratory of Chinese Herbal Drug Discovery Institute of Tropical Medicine Guangzhou University of Chinese Medicine Key Laboratory of Plant Resources Conservation and Sustainable Utilization Guangdong Provincial Key Laboratory of Applied Botany South China Botanical Garden Chinese Academy of Sciences Guangdong Institute of Microbiology

Three new prenylated stilbenes, named as cajanusins A-C(1-3), and one new natural product cajanusin D(4), along with six known derivatives(5-10) were isolated from the leaves of Cajanus cajan. Their structures were fully elucidated by means of extensive spectroscopic methods and comparison with data in the reported literatures. The new compounds of 1 and 2 were evaluated for in vitro cytotoxic activities against a panel of human cancer cell lines.

关键词： Cajanus cajan Stilbenes Flavonoids Leguminosae

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Label-Free Microscopic Imaging Based on the Random Matrix Theory in Wavefront Shaping

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Chinese Physics Letters 2019年第11期36卷 27-30页

作者： Li-Qi Yu xin-Yu Xu Zhen-Feng zhang Qi Feng Bin zhang ying-Chun Ding qiang liu College of Mathematics and Physics Beijing University of Chemical TechnologyBeijing 100029 State Key Laboratory of Precision Measurement Technology and Instruments Department of Precision InstrumentsTsinghua UniversityBeijing 100084

Wavefront shaping technology has mainly been applied to microscopic fluorescence imaging through turbid media,with the advantages of high resolution and imaging depth beyond the ballistic regime. However, fluorescence needs to be introduced extrinsically and the field of view is limited by memory effects. Here we propose a new method for microscopic imaging light transmission through turbid media, which has the advantages of label-free and discretional field of view size, based on transmission-matrix-based wavefront shaping and the random matrix theory. We also verify that a target of absorber behind the strong scattering media can be imaged with high resolution in the experiment. Our method opens a new avenue for the research and application of wavefront shaping.

关键词： theory. matrix absorber

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Saikosaponin a increases interleukin-10 expression and inhibits scar formation after sciatic nerve injury

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Neural Regeneration Research 2018年第9期13卷 1650-1656页

作者： Meng-qiang Huang Xiao-Yu Cao Xu-Yi Chen ying-Fu liu Shuang-Long Zhu Zhong-Lei Sun Xian-Bin Kong Jing-Rui huo Sai zhang Yun-qiang Xu Graduate School Tianjin Medical University Department of Orthopedics Tianjin Medical University General Hospital Department of Rehabilitation Medicine First Affiliated Hospital of Anhui Medical University Tianjin Key Laboratory of Neurotrauma Repair Institute of Traumatic Brain Injury and NeuroscienceCenter for Neurology and Neurosurgery of Affiliated HospitalLogistics University of Chinese People's Armed Police Force Science and Technology Experiment Center Cangzhou Medical College Graduate School Jinzhou Medical University Graduate School Tianjin University of Traditional Chinese Medicine

Nerve scarring after peripheral nerve injury can severely hamper nerve regeneration and functional ***,the anti-inflammatory cytokine,interleukin-10,can inhibit nerve scar *** a（SSa） is a monomer molecule extracted from the Chinese medicine,*** can exert anti-inflammatory effects in spinal cord injury and traumatic brain ***,it has not been shown whether SSa can play a role in peripheral nerve *** this study,rats were randomly assigned to three *** the sham group,the left sciatic nerve was directly sutured after *** the sciatic nerve injury（SNI）＋ SSa and SNI groups,the left sciatic nerve was sutured and continuously injected daily with SSa（10 mg/kg） or an equivalent volume of saline for 7 *** linked immunosorbent assay results demonstrated that at 7 days after injury,interleukin-10 level was considerably higher in the SNI ＋ SSa group than in the SNI *** staining and western blot assay demonstrated that at 8 weeks after injury,type I and III collagen content was lower and nerve scar formation was visibly less in the SNI ＋ SSa group compared with the SNI ***,sciatic functional index and nerve conduction velocity were improved in the SNI ＋ SSa group compared with the SNI *** results confirm that SSa can increase the expression of the anti-inflammatory factor,interleukin-10,and reduce nerve scar formation to promote functional recovery of injured sciatic nerve.

关键词： nerve regeneration saikosaponin a anti-inflammatory factor inflammation interleukin-l O nerve scar peripheral nerve injury sciatic nerve injury sciatic functional index nerve conduction velocity neuroelectrophysiological function neural regeneration

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Polar interaction of polymer host-solvent enables stable solid electrolyte interphase in composite lithium metal anodes

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Journal of Energy Chemistry 2022年第1期31卷 172-178,I0006页

作者： Peng Shi Ze-Yu liu Xue-qiang zhang Xiang Chen Nan Yao Jin Xie Cheng-Bin Jin ying-xin Zhan Gang Ye Jia-Qi Huang Stephens IfanE L Titirici Maria-Magdalena qiang zhang Beijing Key Laboratory of Green Chemical Reaction Engineering and Technology Department of Chemical EngineeringTsinghua UniversityBeijing 100084China Collaborative Innovation Center of Advanced Nuclear Energy Technology Institute of Nuclear and New Energy TechnologyTsinghua UniversityBeijing 100084China Advanced Research Institute of Multidisciplinary Science Beijing Institute of TechnologyBeijing 100081China Shanxi Research Institute for Clean Energy Tsinghua UniversityTaiyuan 030032ShanxiChina Department of Materials Molecular Sciences Research HubImperial College LondonLondon W12 OBZUK epartment of Chemical Engineering Imperial College LondonSouth Kensington CampusLondon SW72AZUK

The lithium(Li) metal anode is an integral component in an emerging high-energy-density rechargeable battery.A composite Li anode with a three-dimensional(3 D) host exhibits unique advantages in suppressing Li dendrites and maintaining dimensional ***,the fundamental understanding and regulation of solid electrolyte interphase(SEI),which directly dictates the behavior of Li plating/stripping,are rarely researched in composite Li metal ***,the interaction between a polar polymer host and solvent molecules was proposed as an emerging but effective strategy to enable a stable SEI and a uniform Li deposition in a working *** carbonate molecules in electrolytes are enriched in the vicinity of a polar polyacrylonitrile(PAN) host due to a strong dipole-dipole interaction,resulting in a LiF-rich SEI on Li metal to improve the uniformity of Li deposition.A composite Li anode with a PAN host delivers 145 cycles compared with 90 cycles when a non-polar host is ***,60 cycles are demonstrated in a 1:0 Ah pouch cell without external *** work provides a fresh guidance for designing practical composite Li anodes by unraveling the vital role of the synergy between a 3 D host and solvent molecules for regulating a robust SEI.

关键词： Lithium metal Polar interaction Solid electrolyte interphase Lithium plating Composite anode

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