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Evolutionary Diversity of Coxsackievirus A6 Causing Severe Hand,Foot,and Mouth Disease—China,2012-2023

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China CDC weekly 2024年第20期6卷 442-449页

作者： huanhuan lu Jinbo Xiao Wenhui Wang Dongmei Yan Tianjiao Ji Qian Yang Haiyan Wei Yanhua Du Yunting Zeng Jun Guo Jianhua Chen Hanri Zeng Yingying Liu Shuaifeng Zhou Hong Ji Jianxing Wang Xiaofang Zhou Yong Zhang National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases World Health Organization Polio Reference Laboratory for the Western Pacific RegionKey Laboratory of Laboratory BiosafetyNational Health and Key Laboratory of Laboratory Biosafety of the National Health CommissionNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Linyi Center for Disease Control and Prevention Linyi CityShandong ProvinceChina Henan Provincial Center for Disease Control and Prevention Zhengzhou CityHenan ProvinceChina Shaanxi Provincial Center for Disease Control and Prevention Xi’an CityShaanxi ProvinceChina Hainan Provincial Center for Disease Control and Prevention Haikou CityHainan ProvinceChina Guizhou Provincial Center for Disease Control and Prevention Guiyang CityGuizhou ProvinceChina Gansu Provincial Center for Disease Control and Prevention Lanzhou CityGansu ProvinceChina Guangdong Provincial Center for Disease Control and Prevention Guangzhou CityGuangdong ProvinceChina Hebei Provincial Center for Disease Control and Prevention Shijiazhuang CityHebei ProvinceChina Hunan Provincial Center for Disease Control and Prevention Changsha CityHunan ProvinceChina Jiangsu Provincial Center for Disease Control and Prevention Nanjing CityJiangsu ProvinceChina Shandong Provincial Center for Disease Control and Prevention Jinan CityShandong ProvinceChina Yunnan Provincial Center for Disease Control and Prevention Kunming CityYunnan ProvinceChina

Introduction:Coxsackievirus A6(CVA6)has emerged as a significant pathogen responsible for severe cases of hand,foot,and mouth disease(HFMD).This study aims to delineate the demographic characteristics and analyze the viral evolution of severe HFMD associated with CVA6,thereby assisting in its surveillance and ***:In this investigation,74 strains of CVA6 were isolated from samples collected from severe HFMD cases between 2012 and *** VP1 gene sequences of CVA6 were amplified and analyzed to assess population historical dynamics and evolutionary characteristics using BEAST,DnaSP6,and ***:A significant portion(94.4%)of severe CVA6-associated HFMD cases(51 out of 54,with 20 lacking age information)were children under 5 years *** the 74 CVA6 strains analyzed,72 belonged to the D3a sub-genotype,while only two strains were D2 *** average genetic distance between VP1 sequences prior to 2015 was 0.027,which increased to 0.051 when compared to sequences *** population dynamics analysis indicated three significant population expansions of severe CVA6-associated HFMD during 2012-2013,2013-2014,and 2019-2020,resulting in the formation of 65 distinct *** with the MCC tree findings,transitioning between regional haplotypes required multiple base substitutions,showcasing an increase in population diversity during the evolutionary process(from 14 haplotypes in 2013 to 55 haplotypes over the subsequent decade).Conclusions:CVA6,associated with severe HFMD,is evolving and presents a risk of outbreak ***,enhanced surveillance of severe HFMD is imperative.

关键词： thereby subsequent finding

Preparation and immunoprotective effects of a virus-like particle candidate vaccine of the dominant epidemic D3 genotype coxsackievirus A6 in China

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Biosafety and Health 2024年第1期6卷 28-34页

作者： Xiaoliang Li Xizhu Xu Jichen Li huanhuan lu Congcong Wang Rui Wang Jinbo Xiao Ying Liu Yang Song Jingdong Song Qiang Sun Yong Zhang Department of Public Health and Health Administration Shandong First Medical UniversityJinan 250117China National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases(NITFID) National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China National Polio Laboratory WHO WPRO Regional Polio Reference LaboratoryNational Health Commission Key Laboratory for BiosafetyNational Health Commission Key Laboratory for Medical VirologyNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBejing 102206China Department of Medical Microbiology Weifang Medical UniversityWeifang 261053China

Coxsackievirus A6 of the D3a genotype(CVA6 D3a)is a primary pathogen causingmainland of China's hand,foot,and mouth disease(HFMD).Viral‐like particle(VLP)vaccines represent a potential candidate vaccine to prevent *** study collected Anti‐CVA6 D3a VLPs serum from BALB/c female mice immunized using CVA6 D3a *** neutralizing antibody levels were compared against the representative 14‐JX2018(D3a)and N4‐YN2015(D3b)strains between the antisera of different immune *** immunoprotective effect of anti‐CVA6 D3a VLPs against these strains was monitored using pathological sections and immuno-histochemical results of lung and skeletal muscle tissues in seven‐day‐old Institute of Cancer Research(ICR)*** protection against different branches of viruses was evaluated in 7‐day‐old(serum passive immune protection)and 14‐day‐old(VLPs active immune protection)neonatal ICR mice ***‐neutralizing antibody levels were positively correlated with the number of immunizations and higher against 14‐JX2018 than against N4‐***,these levels were significantly higher with abdominal injection than intramuscular *** immunized serum of 7‐day‐old ICR mice inoculated three times was 100%protected against CVA6 D3a 14‐JX2018(lethal titer:106.25 TCID 50)and CVA6 D3b N4‐YN2015(lethal titer:105.25TCID 50)fatal attacks,*** ICR mice that have completed two active immunizations for 14 days,both CVA6 D3a 14‐JX2015(challenge titer:108.25 TCID 50)and CVA6 D3b N4‐YN2015(challenge titer:107.25 TCID 50)were used for the challenge,and the mice were able to ***,the CVA6 D3a VLPs prepared in this study are a potential vaccine candidate for CVA6,as it has the optimal protective effect against both CVA6 D3a and D3b evolutionary branches viruses.

关键词： Coxsackievirus A6 D3 genotype Virus‐like particle Immunoprotective effect

日光温室作物冠层夏季光照强度与空气温湿度时空变化规律研究

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农业机械学报 2024年第5期55卷 368-378页

作者：吕欢欢牛源艺韩雨晓李顺达张漫李寒中国农业大学智慧农业系统集成研究教育部重点实验室北京100083

针对温室内不同时间、位置的环境参数存在变异性,且随天气与季节变化,日光温室冠层光照强度、空气温度和空气相对湿度的分布差异性问题,构建了基于无线传感器网络的环境监测系统。环境感知节点部署在作物冠层位置,集成了光照强度、空气... 详细信息

针对温室内不同时间、位置的环境参数存在变异性,且随天气与季节变化,日光温室冠层光照强度、空气温度和空气相对湿度的分布差异性问题,构建了基于无线传感器网络的环境监测系统。环境感知节点部署在作物冠层位置,集成了光照强度、空气温湿度等传感器。首先,基于实时采集的温室环境数据,采用反距离加权算法进行插值分析,得到环境参数的离散曲面;其次,通过基于质心坐标的K-means聚类算法,得到了温室内连通及非连通区域的代表性特征点;最后,采用半变异函数与变异系数方法对温室环境的空间变异性与时间变异性进行分析。实验结果表明,夏季日光温室在下午表现为高温与高光照,08:00、16:00的光照强度分别为12:00的24.2%、72.9%,08:00的空气温度(27.7℃)较12:00、16:00低约6.0℃,对应的空气湿度(90%)高约30%。晴天最大光照强度分别为阴天、雨天的1.4倍和4.6倍,晴天、阴天最高空气温度高于雨天(29.5℃)约6℃,最小空气相对湿度远低于雨天(84%)。夏季日光温室晴天与阴天表现为高温和低湿,雨天表现为高湿和低光照。各环境参数中,光照强度的空间变异性最强,变程为10.34 m。空气温湿度的空间变异性较弱,整体分布均匀。光照强度、空气温度和空气相对湿度的时间变异性均为中等变异程度。环境参数的特征点及时空变化规律有助于日光温室传感器的高效部署,为揭示作物与环境的交互作用提供了基础。

关键词：日光温室光照强度空气温湿度时空变化 K-means 无线传感器网络

半监督空谱局部判别分析的高光谱影像特征提取

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液晶与显示 2024年第2期39卷 131-145页

作者：吕欢欢黄煜铖张辉王雅莉辽宁工程技术大学软件学院辽宁葫芦岛125105 湖州师范学院信息工程学院浙江湖州313000

为充分利用高光谱影像中蕴含的空谱特征,提出了一种半监督空谱局部判别分析的高光谱影像特征提取算法(S4LFDA)。鉴于高光谱数据集具有空间一致性,首先将像元进行空间重构,保存高光谱数据的近邻关系;其次引入光谱信息散度重构像元间的相... 详细信息

为充分利用高光谱影像中蕴含的空谱特征,提出了一种半监督空谱局部判别分析的高光谱影像特征提取算法(S4LFDA)。鉴于高光谱数据集具有空间一致性,首先将像元进行空间重构,保存高光谱数据的近邻关系;其次引入光谱信息散度重构像元间的相似度;为了充分利用大量无标签样本提高算法性能,采用模糊C均值聚类算法对样本进行聚类分析得到伪标签;然后通过增加规范化项到局部力导引算法(FDA)的类内散度矩阵和类间散度矩阵中,以此保持无标签样本的聚类结构一致性;最后通过局部FDA算法来保持有标签样本类间散度最大化和类内散度最小化并求解最佳投影向量。S4LFDA算法既保持了数据集在光谱域的可分性,又保持了像元在空间区域内的近邻关系,合理利用有标签样本及无标签样本,提高了算法的分类性能。在Pavia University和Indian Pines数据集上进行实验,总体分类精度达到95.60%和94.38%。与其他维数约简算法相比,该算法有效提高了地物分类性能。

关键词：高光谱影像半监督空谱判别分析特征提取地物分类

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快递包装循环共用标准体系建设研究

物流科技 2024年第11期47卷 48-50页

作者：路欢欢宋敏上海市质量和标准化研究院上海200031

文章在快递包装循环共用概念及相关方研究的基础上,分析快递包装循环共用的标准化需求,提出快递包装循环共用标准体系框架及实施的对策建议。

关键词：快递包装循环共用标准体系

优化个体护理对支气管哮喘患儿生理健康状况的影响

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中西医结合护理（中英文） 2024年第1期10卷 9-12页

作者：沈琴卢欢欢苏州大学附属儿童医院江苏苏州215000

目的探讨优化个体护理应用于支气管哮喘患儿对其生理健康状况的影响。方法收集2021年1月至2022年12月苏州大学附属儿童医院收治的348例支气管哮喘患儿的一般资料并开展回顾性分析,依据不同的护理方式分为对照组(173例)和观察组(175例)... 详细信息

目的探讨优化个体护理应用于支气管哮喘患儿对其生理健康状况的影响。方法收集2021年1月至2022年12月苏州大学附属儿童医院收治的348例支气管哮喘患儿的一般资料并开展回顾性分析,依据不同的护理方式分为对照组(173例)和观察组(175例)。对照组行常规护理,观察组行优化个体护理。比较2组患儿的生理健康状况,包括肺功能指标、血气分析指标、临床相关指标及炎症因子水平。结果观察组的肺部啰音及喘憋消失时间均较对照组更早,住院时间较对照组更短(P均<0.05)。出院时及出院2周后,观察组的用力肺活量、一秒率、呼气高峰流量和动脉血氧分压均明显较同期对照组更高,动脉血二氧化碳分压、白介素-6、白介素-10和超敏C反应蛋白水平均明显较同期对照组更低(P均<0.05);2组的上述指标均较入院时更优(P均<0.05)。结论在支气管哮喘患儿中实施优化个体护理,可有效改善患儿的临床症状、肺功能及血气分析指标,降低其机体炎症水平,促进患儿康复。

关键词：小儿支气管哮喘优化个体护理肺功能炎症因子

A new approach of using Lorentz force to study single-asperity friction inside TEM

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Journal of Materials Science & Technology 2021年第25期84卷 43-48页

作者： huanhuan lu Zhangjie Wang Di Yun Ju Li Zhiwei Shan Center for Advancing Materials Performance from the Nanoscale(CAMP-Nano)and Hysitron Applied Research Center in China(HARCC) State Key Laboratory for Mechanical Behavior of MaterialsXi’an Jiaotong UniversityXi’an710049People’s Republic of China School of Nuclear Science and Technology Xi’an Jiaotong UniversityXi’an710049People’s Republic of China Department of Nuclear Science and Engineering and Department of Materials Science and Engineering Massachusetts Institute of TechnologyCambridgeMA02139USA

Taking advantage of the magnetic field inside transmission electron microscope(TEM),a unique Lorentzforce-actuated method for quantitative friction tests was developed via a commercial electromechanical *** this approach,a submicron-sized silver asperity sliding on a tungsten flat punch was actuated by Lorentz force due to electrical current through the punch,with the normal force imposed by the built-in transducer of the *** friction force was determined by tracking the elastic deflection of the fabricated cantilever from in situ *** correlating the friction behavior with the microstructural evolution near the silver-tungsten interface,we revealed that even when the relative motion commenced with the plastic deformation of the silver asperity,the interface can still sustain the further increasing static friction *** following the arrival of the maximum static friction force,the sliding occurred at the interface,indicating the transition from static to dynamic *** work enriches our understanding of the underlying physics of the dynamic friction process for metallic friction behavior.

关键词： friction deformation silver

2012-2023年882例重症手足口病病例病原谱及CV-A6基因特征分析

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病毒学报 2024年第2期40卷 383-391页

作者：路环环肖金波严冬梅冀天娇杨倩祝双利张勇传染病溯源预警和智能决策全国重点实验室中国疾病预防控制中心病毒病预防控制所北京102206 国家脊髓灰质炎实验室世界卫生组织西太平洋区脊髓灰质炎参比实验室中国疾病预防控制中心病毒病预防控制所北京102206 国家卫生健康委员会生物安全重点实验室中国疾病预防控制中心病毒病预防控制所北京102206

为了解重症手足口病(Hand,foot,and mouth disease,HFMD)病原谱变化情况以及重症HFMD相关柯萨奇病毒A组6型(Coxsackievirus A6, CVA6)的基因特征,本研究收集了2012-2023年各省级HFMD监测网络实验室送检的882份重症HFMD病例病毒分离物,... 详细信息

为了解重症手足口病(Hand,foot,and mouth disease,HFMD)病原谱变化情况以及重症HFMD相关柯萨奇病毒A组6型(Coxsackievirus A6, CVA6)的基因特征,本研究收集了2012-2023年各省级HFMD监测网络实验室送检的882份重症HFMD病例病毒分离物,通过扩增病毒的VP1编码区进行分子分型以确定肠道病毒的血清型,并对其中的CVA6进行全长VP1区扩增和核苷酸序列测定分析,进行最大似然法系统进化树和氨基酸置换熵值等生物信息学分析。结果发现,2018年开始,以CVA6和CVA10为主的肠道病毒逐渐取代EV-A71和CV-A16成为导致重症HFMD的主要病原体,在每年检出的病原体中的比例均超过50.0%。除2013年重症HFMD中分离的CVA6属于D2基因亚型外,2013年后重症HFMD中分离的CVA6均属于D3a基因亚型,但2019年以后分离的CVA6序列与以往分离株序列的进化距离明显增大。此外,针对氨基酸位点的置换熵值研究表明,参与构成构象表位的VP1-283易发生突变,该位点从2017年开始由亲水的苏氨酸突变成疏水的丙氨酸,导致其可能分别与VP3-57N、VP3-66R之间形成氢键,这可能会影响到病毒的感染过程。本研究明确了2012-2023年中国重症HFMD病原谱的变化特征,探究了导致重症HFMD的CVA6的进化特点以及重要氨基酸变异特征,为重症HFMD的预防控制策略的制定提供了理论依据。

关键词：重症手足口病病原谱柯萨奇病毒A组6型系统进化氨基酸置换熵值

The Dynamic Mortise-and-Tenon Interlock Assists Hydrated Soft Robots Toward Off-Road Locomotion

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Research 2023年第1期2022卷 553-564页

作者： Baoyi Wu Yaoting Xue Israt Ali huanhuan lu Yuming Yang Xuxu Yang Wei lu Yinfei Zheng Tao Chen Key Laboratory of Marine Materials and Related Technologies Zhejiang Key Laboratory of Marine Materials and Protective TechnologiesNingbo Institute of Material Technology and EngineeringChinese Academy of SciencesNingbo 315201China School of Chemical Sciences University of Chinese Academy of Sciences19A Yuquan RoadBejing 100049China Department of Engineering Mechanics Zhejang UniversityHangzhou 310027China INRS-EMT 1650 Boul.Lionel BouletVarennes J3X OA1Canada College of Chemical Engineering Ningbo PolytechnicNingbo 315800China Key Laboratory for Biomedical Engineering of Ministry of Education Ministry of China Key Laboratory of Clinical Evaluation Technology for Medical Device of Zhejiang ProvinceCollege of Biomedical Engineering and Instrument ScienceZhejang UniversityHangzhou 310027China Research Center for Humanoid Sensing Zhejang LabHangzhou 311100China

Natural locomotion such as walking,crawling,and swimming relies on spatially controlled deformation of soft tissues,which could allow efficient interaction with the external *** one of the ideal candidates for biomimetic materials,hydrogels can exhibit versatile bionic ***,it remains an enormous challenge to transfer these insitu deformations to locomotion,particularly above complex ***,inspired by the crawling mode of inchworms,an isotropic hydrogel with thermoresponsiveness could evolve to an anisotropic hydrogel actuator via interfacial diffusion polymerization,further evolving to multisection structure and exhibiting adaptive deformation with diverse degrees of ***,a dynamic mortise-and-tenon interlock could be generated through the interaction between the self-deformation of the hydrogel actuator and rough terrains,inducing continual multidimensional locomotion on various artificial rough substrates and natural sandy ***,benefiting from the powerful mechanical energy transfer capability,the crawlable hydrogel actuators could also be utilized as hydrogel motors to activate static cargos to overstep complex terrains,which exhibit the potential application of a biomimetic mechanical discoloration ***,we believe that this design principle and control strategy may be of potential interest to the field of deformable materials,soft robots,and biomimetic devices.

关键词： terrain versatile utilized