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Immobilization and Properties of Lipase from Candida rugosa on Electrospun Nanofibrous Membranes with Biomimetic Phospholipid Moities

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Chemical Research in Chinese Universities 2008年第2期24卷 231-237页

作者： huang xiao-jun YU An-guo GE Dan XU Zhi-kang Institute of Polymer Science Key Laboratory of Macromolecular Synthesis and Functionalization of Ministry of Education State Key Laboratory of Chemical Engineering Zhejiang University Hangzhou 310027 P. R. China

Reported here is a protocol to fabricate a biocatalyst with high enzyme loading and activity retention, from the conjugation of electrospun nanofibrous membrane having biomimetic phospholipid moiety and lipase. To improve the catalytic efficiency and activity of the immobilized enzyme, poly（acrylonitrile-co-2-methacryloyloxyethyl phosphorylcholine）s（PANCMPCs） were, respectively, electrospun into nanofibrous membranes with a mean diameter of 90 nm, as a support for enzyme immobilization. Lipase from Candida rugosa was immobilized on these nanofibrous membranes by adsorption. Properties of immobilized lipase on PANCMPC nanofibrous membranes were compared with those of the lipase immobilized on the polyacrylonitrile（PAN） nanofibrous and sheet membranes, respectively. Effective enzyme loading on the nanofibrous membranes was achieved up to 22.0 mg/g, which was over 10 times that on the sheet membrane. The activity retention of immobilized lipase increased from 56.4% to 76.8% with an increase in phospholipid moiety from 0 to 9.6%（molar fraction） in the nanofibrous membrane. Kinetic parameter Km was also determined for free and immobilized lipase. The Km value of the immobilized lipase on the nanofibrous membrane was obviously lower than that on the sheet membrane. The optimum pH was 7.7 for free lipase, but shifted to 8.3-8.5 for immobilized lipases. The optimum temperature was determined to be 35 ℃ for the free enzyme, but 42-44℃ for the immobilized ones, respectively. In addition, the thermal stability, reusability, and storage stability of the immobilized lipase were obviously improved compared to the free one.

关键词： Biomimetic polymer Nanofibrous membrane Electrospinning Enzyme immobilization Lipase

Electrospun Poly(acrylonitrile-co-acrylic acid) Nanofibrous Membranes for Catalase Immobilization:Effect of Porphyrin Filling on the Enzyme Activity

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Chemical Research in Chinese Universities 2011年第2期27卷 339-344页

作者： KE Bei-bei WAN Ling-shu huang xiao-jun XU Zhi-kang Key Laboratory of Macromolecular Synthesis and Functionalization Ministry of EducationDepartment of Polymer Science and Engineering Zhejiang University Hangzhou 310027 P. R. China

Porphyrin-filled nanofibrous membranes were facilely prepared by electrospinning of the mixtures of poly（acrylonitrile-co-acrylic acid）（PANCAA） and porphyrins. 5,10,15,20-Tetraphenylporphyrin（TPP） and its metal-loderivatives（ZnTPP and CuTPP） were studied as filling mediators for the immobilization of redox enzyme. Results indicate that the introduction of TPP, ZnTPP and CuTPP improves the retention activity of the immobilized catalase. Among these three porphyrins, the ZnTPP-filled PANCAA nanofibrous membrane exhibits an activity retention of 93%, which is an exciting improvement. This improvement is attributed to both the strong catalase-porphyrin affinity and the possible facilitated electron transfer induced by the porphyrin as evidenced by quartz crystal microbalance （QCM） and fluorescence spectroscopy studies.

关键词： Enzyme immobilization Nanofibrous membrane Electrospinning Quartz crystal microbalance(QCM) Porphyrin

HLA-mismatched/haploidentical hematopoietic stem cell transplantation： a field in which Chinese doctors are making great contributions

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Chinese Medical Journal 2010年第10期123卷 1235-1240页

作者： huang xiao-jun Institute of Hematology Peking University People's Hospital Beijing 100044 China

Allogeneic hematopoietic stem cell transplantation .（allo-HSCT） is one of the best, or even the only,option for the cure of leukemia, especially for patients with high risk factors . However, it is limited by the shortage of suitable donors, because only 25%-30% patients can find a human leukocyte antigen （HLA）-identical sibling donor. Although much progress has been made in finding a suitable unrelated donor or unrelated cord blood （uCB） due to the expansion of the worldwide unrelated donor program,

关键词： allogeneic hematopoietic stem cell transplantation haploidentical China

Optical terminal analysis of a multigrating tiled compressor in a PW-class CPA-laser

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Chinese Physics B 2012年第1期21卷 254-259页

作者： Yang Yu-Chuan Luo Hui Wang xiao Li Fu-Quan huang xiao-jun Jing Feng College of Optoelectric Science and Engineering National University of Defense TechnologyChangsha 410073China Research Center of Laser Fusion China Academy of Engineering PhysicsMianyang 621900China

In the highest-power chirped-pulse amplification lasers, the pulse must be stretched in time, amplified, compressed in a grating compressor and subsequently focused by off-axis parabola to obtain a high peak power. In the optical terminal, the temporal and spatial effects of mismatched multigrating tiled compressor on the far-field pulse are critical factors to be analysed. In this paper, a k-space raytracing model is proposed for the temporal and spatial analyses of possible errors in a four-grating single-pass tiled compressor. The results show that the last grating affects mainly the partial focal spot, while the middle two gratings affect the temporal waveform, and the partial focal spot needs much higher error control than that in the temporal domain in a picosecond pulse compression.

关键词： high-power chirped-pulse amplification lasers optical terminal multigrating tiled com-pressor off-axis parabola

A novel 12,23-epoxy dammarane saponin from Panax notoginseng

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Chinese Journal of Natural Medicines 2015年第4期13卷 303-306页

作者： YUAN Cen XU Fa-Xiang huang xiao-jun LI Shao-Ping ZHANG Qing-Wen State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of Macao Institute of Traditional Chinese Medicine and Natural Products Jinan University

The present study investigated the chemical constituents of the roots and rhizomes of Panax *** were isolated by various column chromatographic methods,and their structures were elucidated by the extensive analysis of spectroscopic data and chemical evidences.A novel 12,23-epoxy dammarane-type saponin,named epoxynotoginsenoside A(1),together with four known compounds(2-5),was isolated and characterized.

关键词： Panax notoginseng Dammarane saponin Epoxynotoginsenoside A

Unique hematopoietic stem cell transplantation system in China

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Chinese Medical Journal 2011年第16期124卷 2403-2405页

作者： huang xiao-jun Peking University Institute of Hematology Peking UniversityPeople's Hospital Beijing 100044 China

Allogeneic hematopoietic stem cell transplantation .（allo-HSCT） is an effective, even the only option to cure patients with certain hematological diseases. HSCT has been applied in China for about 30 years, and great improvement has been made during the past decade. A distinctive HSCT system with Chinese characteristics has been gradually stylized, which manifested in the following aspects.

关键词： Unique hematopoietic transplantation

Advance in hematopoietic stem cells transplantation for leukemia

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Chinese Medical Journal 2008年第18期121卷 1763-1765页

作者： huang xiao-jun Institute of Hematology People's Hospital Peking University Beijing 100044 China

uring the past 50 years, intensive studies into the characteristics of hematopoietic stem cell transplantation immunology immunosuppressant and and the emergence of new anti-infective drugs have significantly improved... 详细信息

关键词： stem cell transplantation hematopoietic stem cell transplantation immunology

Current status and development of hematopoietic stem cell transplantation in China： a report from Chinese Hematopoietic Stem Cell Transplantation Register Group

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Chinese Medical Journal 2011年第16期124卷 2548-2555页

作者： XU Lan-ping huang xiao-jun Peking University Institute of Hematology Peking UniversityPeople's Hospital Beijing 100044 China

Allogeneic hematopoietic stem cell transplantation （alIo-HSCT） has three decades history in China. During these periods, the number of HSCT has been increasing, donor and stem cell sources were expanded, indication of diseases and patients for HSCT extended. Forty-two HSCT units offered their data 1-6 times from July 2007 to june 2010. The annual increase rates were 8.8% to 10.8%. Matched sibling donor is 41%, mismatched related/haploidentical donor is 24%, unrelated volunteer donor is 16%, and umbilical cord blood is 2%. The indications of major disease entities are acute myeloid leukemia （AML, 35%）, acute lymphobastic leukemia （ALL, 25%）, chronic myeloid leukemia （CML, 21%）, and myelodysplastic syndrome （MDS, 8%）. The different opinions on the indication of HSCT were supported by some trials, matched/haploidentical HSCT fit for middle or high risk ALL and AML in first complete remission （CR1）, the international prognosis score system （IPSS） -- middle-II/high risk MDS, CML in advanced stage and so on, when patients have no matched sibling donor. In the Peking University Institute of Hematology, Peking University People＇s Hospital, haploidentical HSCT has received a comparable result to matched simbling donor HSCT and unrelated matched donor HSCT; we suggest haploidentical donor might be a routine alternative donor for high-risk patents who need an urgent HSCT without matched related donor in soecial center.

关键词： hematopoietic stem cell transplantation registration unrelated haploidentical

Allogeneic hematopoietic stem cell transplantation for the treatment of chronic myeloid leukemia in the era of tyrosine kinase inhibitors

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Chinese Medical Journal 2013年第4期126卷 768-774页

作者： XU Lan-ping huang xiao-jun People's Hospital and Institute of Hematology Peking University Beijing Key laboratory of Hematopoitic Stem Cell Transplantation Beijing 100044 China

The approval of imatinib in 2001 has changed the landscape of CML management.^-3 With its excellent efficacy in the International Interferon and STI571 （IRIS）, Randomized Study of imatinib has become the standard of care for newly diagnosed patients. This change challenges the previous role of allogeneic hematopoietic stem cell transplantation （allo-HSCT） in CML treatment. However,

关键词： allogeneic hematopoietic stem cell transplantation chronic myeloid leukemia tyrosine kinase inhibitors," imatinib