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Knock-in of exogenous sequences based on CRISPR/Cas9 targeting autosomal genes and sex chromosomes in the diamondback moth,Plutella xylostella

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Journal of Integrative Agriculture 2024年第9期23卷 3089-3103页

作者： Shanyu Li Guifang Lin Haoqi Wen Haiyan Lu Anyuan Yin Chanqin Zheng Feifei Li Qingxuan Qiao Lu Jiao Ling Lin Yi Yan Xiujuan Xiang huang liao Huiting Feng Yussuf Mohamed Salum Minsheng You Wei Chen Weiyi He State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops/Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops/International Joint Research Laboratory of Ecological Pest Control Ministry of EducationInstitute of Applied EcologyFujian Agriculture and Forestry UniversityFuzhou 350002China Ganzhou Key Laboratory of Greenhouse Vegetable Gannan Normal UniversityGanzhou 341000China

Genetic pest control strategies based on precise sex separation and only releasing sterile males can be accomplished by site-specific genome *** the current study,we showed that the mutation of single-allele Pxfl(2)d can significantly impair the normal mating behavior and testis development in male adults of the notorious cruciferous insect pest Plutella xylostella,in addition to its known functions in the ovarian development in female adults and egg *** CRISPR/Cas9-based knock-in experiments revealed that site-specific integration of an exogenous green fluorescent protein(GFP)gene into autosomal Pxfl(2)d for labelling mutants could be ***,this gene is not a suitable target for GFP insertion to establish a genetically stable knock-in strain because of the severe decline in reproductive *** further screened for the W-chromosome-linked and Z-chromosome-linked regions to test the knock-in efficiency mediated by CRISPR/*** results verified that both types of chromosomes can be targeted for the site-specific insertion of exogenous *** ultimately obtained a homozygous knock-in strain with the integration of both Cas9 and cyan fluorescent protein(CFP)expression cassettes on a Z-linked region in ***,which can also be used for early sex *** injecting the sgRNA targeting Pxfl(2)d alone into the eggs laid by female adults of the Z-Cas9-CFP strain,the gene editing efficiency reached 29.73%,confirming the success of expressing a functional Cas9 *** together,we demonstrated the feasibility of the knock-in of an exogenous gene to different genomic regions in ***,while the establishment of a heritable strain required the positioning of appropriate *** study provides an important working basis and technical support for further developing genetic strategies for insect pest control.

关键词： cruciferous specialist fl(2)d CRISPR/Cas9 knock-in sex chromosome

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Potential of Pteris vittata L. for phytoremediation of sites co-contaminated with cadmium and arsenic: The tolerance and accumulation

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Journal of Environmental Sciences 2008年第1期20卷 62-67页

作者： XIAO Xiyuan CHEN Tongbin AN Zhizhuang LEI Mei huang Zechun liao Xiaoyong LIU Yingru Center for Environmental Remediation Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences Beijing 100101 China.

Field investigation and greenhouse experiments were conducted to study the tolerance of Pteris vittata L. （Chinese brake） to cadmium （Cd） and its feasibility for remediating sites co-contaminated with Cd and arsenic （As）. The results showed that P. vittata could survive in pot soils spiked with 80 mg/kg of Cd and tolerated as great as 301 mg/kg of total Cd and 26.8 mg/kg of diethyltriaminepenta acetic acid （DTPA）-extractable Cd under field conditions. The highest concentration of Cd in fronds was 186 mg/kg under a total soil concentration of 920 mg As/kg and 98.6 mg Cd/kg in the field, whereas just 2.6 mg/kg under greenhouse conditions. Ecotypes of P. vittata were differentiated in tolerance and accumulation of Cd, and some of them could not only tolerate high concentrations of soil Cd, but also accumulated high concentrations of Cd in their fronds. Arsenic uptake and transportation by P. vittata was not inhibited at lower levels （〈20 mg/kg） of Cd addition. Compared to the treatment without addition of Cd, the frond As concentration was increased by 103.8% at 20 mg Cd/kg, with the highest level of 6434 mg/kg. The results suggested that the Cd-tolerant ecotype of P. vittata extracted effectively As and Cd from the site co-contaminated with Cd and As, and might be used to remediate and revegetate this type of site.

关键词： arsenic (As) cadmium (Cd) Chinese brake (Pteris vittata L.) hyperaccumulator phytoremediation tolerance uptake

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麻雀搜索算法优化极端梯度提升模型的岩石爆破块度预测

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科学技术与工程 2024年第24期24卷 10212-10219页

作者：张朋超赵有明刘翔廖黄正何秋芝易泽邦广西科技大学经济与管理学院柳州545006 柳州威宇爆破工程有限责任公司柳州545002 广西工业高质量发展研究中心柳州545006 桂林理工大学地球科学学院桂林541004

为进一步提高岩石爆破块度预测效果,利用多个矿山的岩石爆破统计数据,通过优化极端梯度提升模型(extreme gradient boosting, XGBoost)超参数,建立一种基于随机森林(random forest, RF)特征选择的麻雀搜索算法(sparrow search algorithm... 详细信息

为进一步提高岩石爆破块度预测效果,利用多个矿山的岩石爆破统计数据,通过优化极端梯度提升模型(extreme gradient boosting, XGBoost)超参数,建立一种基于随机森林(random forest, RF)特征选择的麻雀搜索算法(sparrow search algorithm, SSA)优化XGBoost爆破块度预测模型。利用麻雀搜索算法对XGBoost模型决策树数量、决策树最大深度、学习率3个核心超参数进行优化以提高运行效率;利用随机森林对输入特征进行筛选,并将优化后的特征集输入预测模型。结果表明:经特征集优化的模型,爆破块度预测效果整体上更加逼近实际值,且预测结果的可决系数(R-squared,R~2)、均方根误差(root mean square error, RMSE)和平均绝对误差(mean absolute error, MAE)分别为0.954、0.026和0.020,相较于BP(back propagation)神经网络、随机森林和XGBoost模型的效果更优,在实际应用中更具适用性,能为爆破参数设计和优化提供借鉴。

关键词：麻雀搜索算法(SSA) XGBoost模型爆破块度预测

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STUDY ON THE ACCOUNT-SHARING STRATEGY FOR MULTI-SERVICES IN IMS ONLINE CHARGING MECHANISM

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Journal of Electronics(China) 2009年第4期26卷 456-465页

作者： huang Hai liao Jianxin Zhu Xiaomin State Key Laboratory of Networking and Switching Technology Beijing University of Posts and Telecommunications Beijing 100876 China

A prepaid subscriber is allowed to simultaneously implement multiple services in online charging mechanism of IP Multimedia Subsystem (IMS). It is a noteworthy discussion to effectively distribute the limited account resources among concurrent services. An account-sharing algorithm is proposed for multi-services,which introduces resource reclamation and redistribution processes based on the resource reservation of standard specifications and dynamically adjusts them according to the changes of Quality of Service (QoS). Three performance indexes are investigated in the simulation experiments, which are average number of accommodated sessions, average number of completed ses- sions, and average number of iterations per accommodated session. The results show that in the normal QoS level, the three indexes of the proposed algorithm averagely increase by 18.7%, 5.4%, and 3.1% compared with the Prepaid Credit Distribution (PCD) algorithm, and by 2.1%, 1.0%, and 1.8% compared with the Prepaid Credit Reclaim (PCR) algorithm. In the poor QoS level, the performance advantages are greater, which averagely increase by 29.1%, 7.1%, and 2.8% compared with PCD, and by 9.4%, 4.1%, and 3.6% compared with PCR.

关键词： IP Multimedia Subsystem (IMS) Online charging Account-sharing of multi-services Resource reclamation and redistribution

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FHL2通过NF-κB信号通路调节THP-1巨噬细胞泡沫化

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基础医学与临床 2024年第2期44卷 204-209页

作者：陈卫卫廖煌史振鸿罗颖海南医学院第二附属医院心血管内科海南海口570216

目的明确4个半LIM结构域蛋白(FHL2)是否能够通过调节核因子κB(NF-κB)信号通路影响巨噬细胞泡沫化。方法构建FHL2过表达质粒及合成FHL2小干扰RNA(si-FHL2),分别转染至人单核/巨噬细胞系THP-1中,Western blot检测FHL2表达;使用氧化低... 详细信息

目的明确4个半LIM结构域蛋白(FHL2)是否能够通过调节核因子κB(NF-κB)信号通路影响巨噬细胞泡沫化。方法构建FHL2过表达质粒及合成FHL2小干扰RNA(si-FHL2),分别转染至人单核/巨噬细胞系THP-1中,Western blot检测FHL2表达;使用氧化低密度脂蛋白(ox-LDL)刺激细胞,ELISA检测细胞IL-6、IL-1β、TNF-α等细胞因子的表达;油红O染色检测细胞泡沫化程度;Western blot检测NF-κB信号通路相关蛋白表达。结果转染过表达FHL2质粒的细胞中FHL2表达量升高(P<0.01),而转染si-FHL2的细胞表达量降低(P<0.05);敲低FHL2能够下调炎性细胞因子的分泌(P<0.01);FHL2下调能够缓解THP-1巨噬细胞泡沫化;同时,FHL2下调抑制NF-κB信号通路的活化(P<0.05),而在过表达FHL2组中结果呈相反趋势。结论下调FHL2的表达可通过抑制NF-κB信号通路活化,降低炎性细胞因子的分泌,缓解巨噬细胞的泡沫化。

关键词： 4个半LIM结构域蛋白(FHL2) NF-κB信号通路 THP-1 巨噬细胞泡沫化

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A bounding surface visco-plasticity model considering generalized spacing ratio of soils

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第5期16卷 1832-1846页

作者： Xiaosen Kang Hongjian liao Qiangbing huang Jianbing Peng Department of Geological Engineering Chang’an UniversityXi’an710054China Department of Civil Engineering Xi’an Jiaotong UniversityXi’an710049China

The non-unique critical state of soils with time-dependent behaviors is a significant issue in geotechnical engineering ***,previous bounding surface plasticity models cannot predict accurately the non-unique critical state of soils,because the distance between the compression line and critical state line charged by strain-rate effect is basically *** fill this gap,a generalized spacing ratio of soils is defined in the elasto-viscoplastic framework,and a bounding surface visco-plasticity model is formulated and verified,which can consider the generalized spacing ***,the generalized spacing ratio of soils reflects the distance between the compression line and the critical state line of soils with time-dependent ***,the generalized spacing ratio is introduced into an improved anisotropic bounding surface.A new expression of the visco-plastic multiplier is derived by solving the consistency equation of an anisotropic bounding *** the expression,a strain rate index is proposed to account for the strain-rate effect on visco-plastic strain increment,and a visco-plastic hardening modulus is derived to predict the visco-plastic response of soils in overconsolidation *** model is then verified through constant strain rate tests and creep ***,it can capture the non-unique critical states of soils with time-dependent behaviors due to the generalized spacing ratio and the creep rupture of soils due to the visco-plastic multiplier that considers the stress ratio and visco-plastic strain rate.

关键词： Soil Constitutive model Visco-plastic behavior Strain rate Creep Anisotropic

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HTDcr:a job execution framework for high-throughput computing on supercomputers

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Science China(Information Sciences) 2024年第1期67卷 69-85页

作者： Jiazhi JIANG Dan huang Hu CHEN Yutong LU Xiangke liao School of Computer Science and Engineering Sun Yat-sen University School of Software Engineering South China University of Technology

High-throughput computing(HTC) is a computing paradigm that aims to accomplish jobs by easily breaking them into smaller,independent ***,it requires a large amount of computing power for a long *** existing HTC frameworks are job-oriented without support for coscheduling with hardware architecture and task-level ***,most of the frameworks reach a limited scale,and their usability needs further ***,we present HTDcr,a job execution framework for the HTC on *** study aims to improve the throughput,task dispatching,and usability of the *** detail,the throughput optimizations include a sophisticated designed task management system,a hierarchical scheduler,and the co-optimization of the task-scheduling strategy with the application and hardware *** optimizations for usability include a programable execution workflow,mechanisms for more robust and reliable service qualities,and a fine-grained resource allocation system for the colocation of multiple *** to our evaluations,HTDcr can achieve outstanding scalability and high throughput on large-scale clusters for the HTC *** evaluate HTDcr with several microbenchmarks and real-world applications on Tianhe-2 and Sunway TaihuLight to demonstrate its effects on existing design *** instance,the task scheduling for two real-world applications integrated with the application and hardware characteristics achieves 1.7× and 1.9× speedups over the basic task-scheduling strategy.

关键词： high-throughput computing supercomputer task scheduling middleware password guessing

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FPR1 Antagonist (BOC-MLF) Inhibits Amniotic Epithelial-mesenchymal Transition

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Current Medical Science 2024年第1期44卷 187-194页

作者： Xiao-mei huang E liao Jun-qun liao Ya-ling LIU Yong SHAO Department of Obstetrics and Gynecology The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400042China Department of Obstetrics Maternal and Child Health Hospital of Hubei ProvinceWuhan 430070China Medical Laboratory Science The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400042China Department of Obstetrics Yubei Maternity and Child Healthcare HospitalChongqing 400042China

Objective:Premature rupture of membranes(PROM)is a common pregnancy disorder that is closely associated with structural weakening of fetal *** have found that formyl peptide receptor 1(FPR1)activates inflammatory pathways and amniotic epithelial-mesenchymal transition(EMT),stimulates collagen degradation,and leads to membrane weakening and membrane *** purpose of this study was to investigate the anti-inflammatory and EMT inhibitory effects of FPR1 antagonist(BOC-MLF)to provide a basis for clinical prevention of ***:The relationship between PROM,FPR1,and EMT was analyzed in human fetal membrane tissue and plasma samples using Western blotting,PCR,Masson staining,and ELISA ***(LPS)was used to establish a fetal membrane inflammation model in pregnant rats,and BOC-MLF was used to treat the LPS rat *** detected interleukin(IL)-6 in blood from the rat hearts to determine whether the inflammatory model was successful and whether the anti-inflammatory treatment was *** used electron microscopy to analyze the structure and collagen expression of rat fetal ***:Western blotting,PCR and Masson staining indicated that the expression of FPR1 was significantly increased,the expression of collagen was decreased,and EMT appeared in *** rat model indicated that LPS caused the collapse of fetal membrane epithelial cells,increased intercellular gaps,and decreased ***-MLF promoted an increase in fetal membrane collagen,inhibited EMT,and reduced the weakening of fetal ***:The expression of FPR1 in the fetal membrane of PROM was significantly increased,and EMT of the amniotic membrane was ***-MLF can treat inflammation and inhibit amniotic EMT.

关键词： formyl peptide receptor 1 BOC-MLF epithelial-mesenchymal transition premature rupture of membranes

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A demand forecasting model for urban air mobility in Chengdu,China

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Green Energy and Intelligent Transportation 2024年第3期3卷 11-23页

作者： Wenqiu Qu Jie huang Chenglong Li Xiaohan liao State Key Laboratory of Resources and Environment Information System Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101China College of Resources and Environment University of Chinese Academy of SciencesBeijing 100101China Key Laboratory of Regional Sustainable Development Modeling Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101China Civil Aviation Flight University of China Guanghan618307China The Research Center for UAV Applications and Regulation CASBeijing 100101China

The successful application of new technologies such as remotely piloted aircraft systems,distributed electric propulsion systems,and automatic control systems on electric vertical take-off and landing(eVTOL)aircraft has prompted Urban Air Mobility(UAM)to be mentioned *** is a newly raised transport mode of using eVTOL aircraft to transport people and cargo in urban areas,which is thought to share some of the traffic on the *** of the prerequisites for UAM to operate on a regular basis is that its demand can support the operating costs,so forecasting UAM demand is *** conduct UAM demand forecasting based on the four-step method,focusing on improving the third-step modal split,and propose a demand forecasting model based on the logit *** model combines a nested logit(NL)model with a multinomial logit(MNL)model to solve the problem of non-existent UAM sharing *** use Chengdu,China as an example,and focus on forecasting the UAM traffic demand in 2030 with the help of the four-step *** results show that UAM is suitable for shared operation during the early *** a fully shared operation,the UAM share rate increases by 0.73%for every kilometer increase in ***,UAM is more competitive than other modes for delivery distances exceeding 15 ***,using the distributions of the share rate and traffic flow pattern from the simulation,we propose the routes that can be prioritized for UAM operations in Chengdu.

关键词： Urban air mobility Four-step method Demand forecasting Logit model Share rate

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Facile synthesis of nanoparticles-stacked Co_(3)O_(4)nanoflakes with catalase-like activity for accelerating wound healing

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Regenerative Biomaterials 2024年第3期3卷 82-91页

作者： Yanan huang Wanyi liao Wenxuan Wang Tingting Zhang Yan Zhang Lei Lu school and Hospital of Stomatology Wenzhou Medical UniversityWenzhou 325027China sichuan Engineering Research Center for Biomimetic Synthesis of Natural Drugs School of Life Science and EngineeringSouthwest jiaotong UniversityChengdu 610031China Key Lab of Advanced Technology of Materials of Education Ministry School of Materials Science and EngineeringSouthwest jiaotong UniversityChengdu 610031China school of Chemistry Southwest Jiaotong UniversityChengduSichuan 610031China

Delayed wound healing caused by excessive reactive oxygen species(Ros)remains a considerable *** recent years,metal oxide nanozymes have gained significant attention in biomedical ***,a comprehensive investigation of Co_(3)O_(4)-based nanozymes for enhancing wound healing and tissue regeneration is *** study focuses on developing a facile synthesis method to produce high-stability and costeffective Co_(3)O_(4)nanoflakes(NFs)with promising catalase(CAT)-like activity to regulate the oxidative microenvironment and accelerate wound *** closely arranged Co_(3)O_(4)nanoparticles(NPs)within the NFs structure result in a significantly larger surface area,thereby amplifying the enzymatic activity compared to commercially available Co_(3)O_(4)*** physiological conditions,itwas observed that CogO,NFs efficientlybreak down hydrogen peroxide(H_(2)O_(2))without generating harmful radicals(OH).Moreover,they exhibit excellent compatibility with various cells involved in wound healing,promoting fibroblast growth and protecting cells from oxidatives *** a ratmodel,Co_(3)O_(4) NFs facilitate both the hemostatic and proliferative phases of wound healing,consequently accelerating the ***,thepromising results of Co_(3)O_(4)NFs highlight their potential in promoting wound healing and tissue regeneration.

关键词： Co_(3)O_(4) nanoflake nanozyxygen species wound healing

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