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补体因子H多态性在年龄相关性黄斑变性发病中的作用机制

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中国组织工程研究与临床康复 2007年第32期11卷 6469-6472页

作者：黄江徐国旭苏州大学附属第二医院眼科中心江苏省苏州市215004

目的:探讨补体H因子的多态性在年龄相关性黄斑变性疾病发生中的作用机制。资料来源:应用计算机检索PubMed数据库1983-01/2006-12相关年龄相关性黄斑变性及补体H因子多态性方面的文献,检索词"Age-related macular degeneration,complemen... 详细信息

目的:探讨补体H因子的多态性在年龄相关性黄斑变性疾病发生中的作用机制。资料来源:应用计算机检索PubMed数据库1983-01/2006-12相关年龄相关性黄斑变性及补体H因子多态性方面的文献,检索词"Age-related macular degeneration,complement factor H,polymorphism,epidemiology,etiology",限定文献语言种类为Engllish。资料选择:对资料进行初审,选取包括年龄相关性黄斑变性流行病学、病因学以及补体H因子多态性方面的文献,开始查找全文。纳入标准:文章所述内容与年龄相关性黄斑变性或补体H因子相关。排除标准:临床诊断及治疗。资料提炼:共检索到176篇相关文献,最终纳入38篇符合标准的文献。资料综合:年龄相关性黄斑变性的发病机制十分复杂,至今尚不清楚。最近研究发现,补体因子H在年龄相关性黄斑变性的发生、发展中起着相当重要的作用,如果补体因子H功能下降,补体被异常激活,导致补体成分C3b、C5b-9产生增多,C5b-9(膜攻击复合体)沉积于视网膜色素上皮细胞基底层,促进玻璃膜疣的形成并沉积于视网膜色素上皮层与Bruch膜之间,引起视网膜色素上皮层-Bruch膜-脉络膜毛细血管复合体变性,黄斑区和后极部萎缩,进而Bruch膜内胶原增厚,弹力纤维层断裂,脉络膜毛细血管进入视网膜色素上皮层下及视网膜神经上皮层下,最终导致视网膜下新生血管的形成,年龄相关性黄斑变性的发生。结论:补体因子H多态性在年龄相关性黄斑变性的发生中起着非常重要的作用,这为今后采取新的早期干预措施、寻求更为有效的治疗方法提供了新的方向。

关键词：年龄相关性黄斑变性补体因子H 多态性综述文献

神经营养因子Neuritin的生物学特性与神经再生

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中国组织工程研究与临床康复 2007年第19期11卷 3813-3816页

作者：李新丽黄谨石河子大学医学院生化教研室

目的:总结Neuritin基因表达调控、分子特征及在神经再生领域的研究。资料来源:应用计算机检索Pubmed和Springlink数据库2005-01/2006-10相关神经营养因子Neuritin、神经再生方面的文献,检索词“Neuritin、nerve regeneration”,限定文... 详细信息

目的:总结Neuritin基因表达调控、分子特征及在神经再生领域的研究。资料来源:应用计算机检索Pubmed和Springlink数据库2005-01/2006-10相关神经营养因子Neuritin、神经再生方面的文献,检索词“Neuritin、nerve regeneration”,限定文献语言种类为English。资料选择:对资料进行初审,选取包括Neuritin的全部文献和神经再生相关的文献,开始查找全文。纳入标准:与神经再生有关的神经营养因子的机制研究。排除标准:神经再生相关的药物及功能研究。资料提炼:在Pubmed共检索到与Neuritin相关的文章17篇,在Springlink检索到49篇与神经再生相关的生物医学和生命科学的文献,最终纳入39篇符合标准的文献。资料综合:Neuritin是一种新近发现的与神经可塑性相关的神经营养因子,能促进树突和轴突的生长和突触成熟,调节突触回路的形成。它是一条编码糖基磷脂酰肌醇锚定的糖蛋白基因,在神经组织中表达最多,其中以齿状回最高,其次为海马,大脑皮层和小脑。Neuritin作为神经活动和神经营养素发挥作用的共同下游因子,在促进突触成熟、防止神经细胞凋亡、保护运动神经元和促进血管生成等方面具有重要作用。本文就Neuritin的生物学特性及在神经再生领域的研究做一综述。结论:Neuritin作为一种新的、与神经可塑性相关的营养因子,在神经再生领域中具有潜在的、良好的应用前景。

关键词： Neuritin 神经可塑性,神经再生综述文献

Novel functional proteins interact with midkine in hepatic cancer cells

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Hepatobiliary & Pancreatic Diseases International 2012年第3期11卷 272-277页

作者： Qiang Yan, Hui-Lian huang, Xing Yao, jing Li, Li-Qin Li, jing Zhong, Li-Shan Min, Li-Cheng Dai and Shu-Sen Zheng Department of Hepatobiliary and Pancreatic Surgery, Huzhou Central Hospital (Yan Q and Yao X) Huzhou Key Laboratory of Molecular Medicine, Affiliated Central Hospital of Huzhou Teachers College (huang HL, Li j, Li LQ, Zhong j, Min LS and Dai LC), Huzhou 313000, China Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Yan Q and Zheng SS) Department of Hepatobiliary and Pancreatic Surgery Huzhou Central Hospital Huzhou 313000 China Division of Hepatobiliary and Pancreatic Surgery First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China Huzhou Key Laboratory of Molecular Medicine Affiliated Central Hospital of Huzhou Teachers College Huzhou 313000 China

BACKGROUND: Midkine is a heparin-binding growth factor that promotes the proliferation, survival, migration and differentiation of various target cells. Midkine plays an important role in tumorigenesis and tumor progression, and is overexpressed in many human malignant tumors. Patients with high tumor midkine expression frequently have a worse prognosis than those with low expression. The present study was designed to investigate the interaction network of midkine in hepatic cancer cells, and to elucidate its role in hepatocellular carcinoma. METHODS: DNA encoding full-length midkine was cloned into pDBLeu vector to serve as bait in yeast two-hybrid screening to identify interacting proteins. Candidate proteins were examined on SC-Leu-Trp-His+3-AT (20 mmol/L) plates and assayed for X-gal activity, then sequenced and classified according to the GenBank. Finally, identified proteins were expressed by the in vitro expression system pCMVTnT, and protein interactions were confirmed by co-immunoprecipitation. RESULTS: Using the yeast two-hybrid system, we found 6 proteins that interacted with midkine: NK-kappa-B inhibitor alpha (I-κ-B-α), Dvl-binding protein naked cuticle 2, granulin, latent active TGF-β binding protein 3, latent active TGF-β binding protein 4, and phospholipid scramblase 1. In vitro co-immunoprecipitation demonstrated that all identified proteins directly interacted with ***: The identification of midkine-interacting proteins in hepatic cancer cells indicates that midkine is a multifunctional factor that may participate in cell migration, differentiation, and proliferation, and is also associated with the multicellular response feedback during the development of hepatocellular carcinoma.

关键词： midkine yeast two-hybrid interaction hepatic cancer cell

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地震是一个确定性混沌的例子吗？

地震译文集 1992年第2期11卷 53-56,F003页

作者： huang,j 周尽

Circulating HER2 extracellular domain(ECD) predicts a poor prognosis for metastatic breast cancer patients

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Circulating HER2 extracellular domain(ECD) predicts a poor p...

Numerical prediction of a bulb turbine performance hill chart through RANS simulations

国家级“乳腺癌诊治新进展”学习班暨学术年会

作者： Shao XY Xu XH Wang S huang j Cai jF Cao WM Chen ZH huang P Lou Cj Ling ZQ Han MX Wang Xj Zhejiang Cancer Research Institute Zhejiang Cancer Hospital

Background:Amplification or overexpression of HER2 has been shown to play an important role in approximately 30%of breast cancers and is strongly associated with increased recurrence and a worse *** HER2 extracellular domain(ECD) may be cleaved and shed from the surface of breast cancer cells and serum HER2 ECD levels can be detected by enzyme-linked immunosorbent assay (ELISA).In this study,we explored the relationship between circulating HER2 ECD and tissue HER2

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Numerical prediction of a bulb turbine performance hill char...

Endoplasmic Reticulum Stress Induced by Tunicamycin and Antagonistic Effect of Tiantai No.1(天泰1号) on Mesenchymal Stem Cells

The 26th IAHR Symposium on Hydraulic Machinery and Systems

作者： V Guénette S Houde G D Ciocan G Dumas j huang C Deschênes Hydraulic Machines Laboratory LAMHFaculty of Science and EngineeringLaval UniversityQuébecCanada Numerical Fluid Mechanics Laboratory LMFNFaculty of Science and EngineeringLaval UniversityQuébecCanada Renewable Energy Technologies CanmetENERGYNatural Resources CanadaOttawaCanada

With in the framework of an international research consortium on low-head hydraulic turbine flow dynamics,the predictive behavior of Reynolds Averaged Navier-Stokes （RANS） simulations of the efficiency （η） hill chart of a bulb turbine is *** paper presents the impacts of the blade tip gap and the hub gaps on performance predictions.

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Chinese journal of Integrative Medicine 2010年第1期16卷 41-49页

作者：吴正治李映红 Andrew C.j.huang 李明张晓丽王济国杨敏陈嫚茵 Shenzhen Hospital of Southern Medical University Larry Hillblom Islet Research Center David Geffen School of MedicineUCLALos AngelesCA 90024USA

Objective： Changes of the internal and external cellular environments can induce calcium homeostasis disorder and unfolded protein aggregation in the endoplasmic reticulum （ER）. This ER function disorder is called endoplasmic reticulum stress （ERS）. Severe long-term ERS can trigger the ER apoptosis signaling pathway, resulting in cell apoptosis and organism injury. Recent researches revealed that ERS-induced cell death was involved in the neurocyte retrogradation in the progress of neuron degenerative diseases, such as Alzheimer＇s disease （AD）, Parkinson＇s disease and so on. Therefore, the protection effect of the traditional Chinese drug Tiantai No. 1 （天泰1号） on the ERS injury of AD was investigated at the molecular gene level in this study with a view to explore the gene pharmacodynamic actions and mechanisms of this drug. Methods： Primarily cultured marrow mesenchymat stem cells （MSCs） of rats were treated by tunicamycin （TM） in order to induce ERS. RT-PCR, fluorescence immunocytochemistry and Western blot techniques were used to determine the mRNA and protein expression levels of the protective stress protein-ER molecular chaperones GRP78 and GRP94 （which would assist cells to resist cellular stress injury）, and to determine the mRNA and protein expression levels of apoptosis promoting molecule Caspase-12 on the membrane of the ER, respectively. Results： Protein expression levels of GRP78 and GRP94 were significantly increased in the TM-induced MSCs, and the mRNA level of Caspase-12 was also remarkably increased in the TM-induced MSCs （P〈0.05）. All these proved that the ERS model was successfully established by TM in MSC. Meanwhile, the mRNA and protein levels of GRP78 and GRP94 were all significantly increased compared with the model group （P〈0.05 or P〈0.01） after MSCs were treated with Tiantai No.1 while the mRNA and protein expression levels of Caspase-12 were significantly decreased compared with the model group （P〈0.05 or P〈0.01）. This effect showed a dose d

关键词： endoplasmic reticulum stress Alzheimer's disease mesenchymal stem cell tunicamycin Tiantai No.1

Reactor Protection Diversity Requirements and the Typical Solution Based on DCS

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journal of Energy and Power Engineering 2011年第12期5卷 1149-1153页

作者： W.j. huang Y.j. Tian X.S. Dong L.M. Zhang G.M. Liu G.j. jiang China Nuclear Power Design Co. L TD Shenzhen 518045 China

The defense-in-depth and diversity have become the basic requirements for digital I＆C and should be strictly followed when carrying out the overall scheme design of digital control system, in which the risk of common cause failure has to be prevented or coped with. This paper analyzes the requirements of the diversity principle and sums up the typical solution of the diversified protection of reactor based on the CPR1000 NPP. Some concepts will be helpful to the design of DCS for others plants.

关键词： Protection defense-in-depth and diversity DCS nuclear power plant.

Neuropeptide cholecystokinin:a key neuromodulator for hippocampal functions

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Neural Regeneration Research 2025年第7期20卷 1991-1992页

作者： Fengwen huang Stephen Temitayo Bello Department of Neuroscience City University of Hong KongKowloon TongHong Kong Special Administrative RegionChina Center for Regenerative Medicine and Health Hong Kong Institute of Science&InnovationChinese Academy of SciencesHong Kong Special Administrative RegionChina

Spatial memory is crucial for survival within external surroundings and wild *** hippocampus,a critical hub for spatial learning and memory formation,has received extensive investigations on how neuromodulators shape its functions(Teixeira et al.,2018;Zhang et al.,2024).However,the landscape of neuromodulations in the hippocampal system remains poorly understood because most studies focus on classical monoamine neuromodulators,such as acetylcholine,serotonin,dopamine,and *** neuropeptides,comprising the most abundant neuromodulators in the central nervous system,play a pivotal role in neural information processing in the hippocampal ***(CCK),one of the most abundant neuropeptides,has been implicated in regulating various physiological and neurobiological statuses(Chen et al.,2019).CCK-A receptor(CCK-AR)and CCK-B receptors(CCK-BR)are two key receptors mediating the biological functions of CCK,both of which belong to class-A sevenfold transmembrane G protein-coupled receptors(Nishimura et al.,2015).CCK-AR preferentially reacts to sulfated CCK,whereas CCK-BR binds both CCK and gastrin with similar affinities(Ding et al.,2022).The expression patterns of CCK-AR and CCK-BR are distinct,implying that CCK has various functions in target *** instance,CCK-AR is widely expressed in the GI and brain subregions and is hence implicated in the control of digestive function and satiety ***,CCK-BR is abundantly and widely distributed in the central nervous system,which majorly regulates anxiety,learning,and memory(Ding et al.,2022).However,the roles of endogenous CCK and CCK receptors in regulating hippocampal function at electrophysiological and behavioral levels have received less attention.

关键词： abundant functions modulator