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Journal of Integrative Agriculture 2016年第3期15卷 702-704页

作者： KONG Ling-an wu Du-qing huanG wen-kun PENG huan HE wen-ting PENG De-liang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences

Soybean cyst nematode Heterodera glycines is one of the most serious soil-borne pathogens in soybean production. However, the researches were limited in China due to lack of an effective pathosystem. In this study, we screened 21 legume Medicago plants in both Medicago truncatula and Medicago sativa to obtain candidate model plants for establishing a new pathosystem for legume-H. glycines interactions. The nematode infection of tested plants was assayed with Race 3 and 4 respectively, which were two dominant H. glycines inbred races in China soybean producing areas. The results showed that the model legume plant M. truncatula A17 failed to allow Race 3 of H. glycines to complete its life cycle, in contrast, it provided the Race 4 population to form several cyst nematodes, however, the female index（FI） value was approximately 1.6. Three M. sativa cultivars, including Xunlu, Aergangjin and Junren, provided either Race 3 or 4 of H. glycines to develop into mature cysts with their FI value below 5 as well. Our results demonstrated that legume plants in both M. truncatula and M. sativa were not likely to be a model plant for H. glycines because of an extreme high resistance.

关键词： soybean cyst nematode alfalfa life cycle female index pathosystem

维氏气单胞菌TH0426胞外产物酶活性及免疫原性分析

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中国兽医学报 2017年第10期37卷 1896-1899页

作者：毕建飞陈龙康元环孙武文吴同垒张海月张冬星孟良良单晓枫钱爱东吉林农业大学动物科学技术学院吉林长春130118 河北科技师范学院河北省预防兽医学重点实验室河北秦皇岛066004

为研究维氏气单胞菌(Aeromonasveronii)胞外产物的合成及其功能,本试验采用超滤浓缩法提取Aeromonasveronii TH0426的胞外产物,通过平板琼脂扩散法对其主要酶成分进行初步研究,对蛋白酶、脂酶、卵磷脂酶、淀粉酶、脲酶、明胶酶和溶血活... 详细信息

为研究维氏气单胞菌(Aeromonasveronii)胞外产物的合成及其功能,本试验采用超滤浓缩法提取Aeromonasveronii TH0426的胞外产物,通过平板琼脂扩散法对其主要酶成分进行初步研究,对蛋白酶、脂酶、卵磷脂酶、淀粉酶、脲酶、明胶酶和溶血活性进行测定,对斑马鱼腹腔注射胞外产物测定其致病性,并对其免疫原性进行初步分析。结果显示,菌株胞外产物具有脂酶、淀粉酶、蛋白酶和溶血活性,人工感染试验结果表明,菌株胞外产物对斑马鱼具有致病性,其半数致死量为3.355 0μg/条。SDS-PAGE表明,ECP由19条蛋白带组成,利用鲤鱼抗ECP血清进行Western blot显示,组成ECP的19条蛋白带中有5条具有免疫原性,能够引发机体的免疫应答反应产生抗体,其相对分子质量为别为110 000,47 000,45 000,42 000和38 000,为Aeromonasveronii胞外产物亚单位疫苗的研制与开发提供理论依据。

关键词： Aeromonasveronii 胞外产物斑马鱼致病性免疫原性

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红曲黄素对蚕丝的染色性能研究

成都纺织高等专科学校学报 2017年第1期34卷 160-164页

作者：皮婷婷黄启帆马辉张焕侠吴雯曹建达嘉兴学院材料与纺织工程学院浙江嘉兴314000

将红曲黄素作为染料上染蚕丝织物,研究红曲黄素的染色特性,确定其染色蚕丝的最佳工艺,以及探究红曲黄素染色后蚕丝织物力学性能上的变化,以期将天然的真菌色素应用至蚕丝织物。结果表明:在较低的温度如85℃就可以实现蚕丝织物的染色,从... 详细信息

将红曲黄素作为染料上染蚕丝织物,研究红曲黄素的染色特性,确定其染色蚕丝的最佳工艺,以及探究红曲黄素染色后蚕丝织物力学性能上的变化,以期将天然的真菌色素应用至蚕丝织物。结果表明:在较低的温度如85℃就可以实现蚕丝织物的染色,从而可降低染色时的能耗;随着染色浓度变化,蚕丝的K/S值会随之增大,在15%(o.w.f)之后,增加速度变缓,即在15%(o.w.f)时就可以达到较高的上染率。

关键词：红曲黄染色蚕丝染色性能

Fabrication of Silver Yolk@Porous Janus Polymer Shell Nanospheres for Synergistic Catalysis

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Chinese Journal of Polymer Science 2020年第8期38卷 847-852,I0007页

作者： Shao-hong Liu Yan-huan Lin wen-tai Guo Shi-mei Li Wei-cong Mai Hui Wang Ruo-wen Fu Ding-cai wu Materials Science Institute PCFM Lab and GDHPPC LabSchool of ChemistrySun Yat-sen UniversityGuangzhou 510275China Department of Colorectal Surgery Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor DiseasesThe Sixth Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510655China

Yolk-shell nanostructures have recently attracted tremendous research interests in various areas because of their unique structural ***,there is an urgent need for developing porous shells with multifunctional features to enhance their performance in various ***,advanced yolk-shell nanospheres have been facilely prepared by encapsulating silver nanoparticles with porous Janus polymer shells that consist of a hypercrosslinked polystyrene(xPS)outer layer and a tethered poly(acrylic acid)(PAA)brush *** xPS outer layer possesses well-developed porosity as mass diffusion *** importantly,the tethered PAA brushes with customized carboxyl groups exhibit great affinity toward specific species(e.g.,cationic dyes),leading to their enrichment from the bulk solution into the interior *** unusual combination of highly porous outer layers with customizable inbuilt polymer linings in the porous Janus shells endows them with great promise for synergistically catalytic degradation of cationic dyes.

关键词： Yolk-shell nanostructure Janus shell Selective diffusion Synergistic catalysis

Anti-obesity and Gut Microbiota Modulation Effect of Astragalus Polysaccharides Combined with Berberine on High-Fat Diet-Fed Obese Mice

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Chinese Journal of Integrative Medicine 2023年第7期29卷 617-625页

作者： YUE Shi-jun WANG wen-xiao ZHANG Lei LIU Juan FENG wu-wen GAO huan TANG Yu-ping YAN Dan Beijing Key Laboratory of Bio-characteristic Profiling for Evaluation of Rational Drug Use Beijing Shijitan HospitalCapital Medical UniversityBeijing100038China Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility Shaanxi University of Chinese MedicineXi’an712046China Department of Pharmacy Beijing Friendship HospitalCaptical UniversityBeijing Institute of Clinical PharmacyBeijing100050China

Objective To investigate whether astragalus polysaccharides(APS)combined with berberine(BBR)can reduce high-fat diet(HFD)-induced obesity in *** Except for normal mice,32 HFD-induced obese mice were randomized into HFD,APS(1,000 mg/kg APS),BBR(200 mg/kg BBR),and APS plus BBR(1,000 mg/kg APS plus 200 mg/kg BBR)groups,*** 6-week treatment(once daily by gavage),the obesity phenotype and pharmacodynamic effects were evaluated by histopathological examination of epididymal fat,liver,and colon using hematoxylin-eosin staining and serum biochemical analyses by an automated chemistry *** feces were collected at the 12 th week,and taxonomic and functional profiles of gut microbiota were analyzed by 16S ribosomal ribonucleic acid(16S rRNA)*** Compared with HFD group,the average body weight of APS plus BBR group was decreased(P<0.01),accompanied with the reduced fat accumulation,enhanced colonic integrity,insulin sensitivity and glucose homeostasis(P<0.05 or P<0.01).Importantly,APS combined with BBR treatment was more effective than APS or BBR alone in improving HFD-induced insulin resistance(P<0.05 or P<0.01).16S rRNA sequence-based analysis of fecal samples demonstrated that APS combined with BBR treatment exhibited a better impact on HFD-induced gut microbiota dysbiosis,exclusively via the enriched abundances of Bacteroides,which corresponded to the large increase of predicted bacterial genes involved in carbohydrate *** APS combined with BBR may synergistically reduce obesity and modulate the gut microbiota in HFD-fed mice.

关键词： Astragalus polysaccharides berberine obesity gut microbiota Bacteroides

Magnetic resonance cholangiopancreatography for the detection of pancreatic duct stones in patients with chronic pancreatitis

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World Journal of Gastroenterology 2009年第20期15卷 2543-2546页

作者： Zhen-Hua Ma Qing-Yong Ma huan-Chen Sha Sheng-Li wu Jun wen Department of Hepatobiliary Surgery the First Affiliated Hospital Medical School of Xi'an Jiaotong University Xi'an 710061 Shaanxi Province China

AIM:To assess the role of magnetic resonance cholangiopancreatography(MRCP) in detection of pancreatic duct stones(PDS) in patients with chronic pancreatitis(CP).METHODS:Clinical data of 78 CP patients who were treated at the First Affi liated Hospital of Xi'an Jiaotong University(China) between January 2004 and July 2008 were retrospectively analyzed.A predictive model of pancreatic duct stones was established through logistic regression and its effectiveness was verifi *** these patients, MRCP was performed in 60 patients who served as a control group, while 44 patients with a higher predictive value than the entry threshold of the predictive model served as an experimental ***:The positive rate of PDS in the 78 patients with CP was 19.2%(15/78).The predictive entry threshold of the predictive model was 5%(P < 0.05).The possibility of existence of PDS could be predicted according to the following 4 indexes:gastrointestinal symptoms, intermittent abdominal pain, diabetes mellitus(DM)/impaired glucose tolerance(IGT) and positive B-mode ultrasound *** incidence of PDS in the experimental group was higher than that in the control group(P < 0.05).CONCLUSION:MRCP is strongly suggested for the detection of PDS in patients with gastrointestinal symptoms, intermittent abdominal pain, DM/IGT and positive B-mode ultrasound results.

关键词： Chronic pancreatitis Pancreatic duct stone Magnetic resonance cholangiopancreatography B-mode ultrasound Logistic regression

Upconversion Emission Properties of Erbium-and Ytterbium-Doped Potassium Lithium Tantalate Niobate Ceramics

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Chinese Physics Letters 2013年第12期30卷 116-119页

作者： LI Lei ZHOU Zhong-Xiang YANG wen-Long LI huan wu Ye Jiangsu Key Laboratory of Power Transmission and Distribution Equipment Technology Hohai UniversityChangzhou 213022 Department of Physics Harbin Institute of TechnologyHarbin 150001

We investigate the luminescence properties of erbium-doped and erbium-ytterbium-codoped potassium lithium tantalate niobate ceramics prepared by the solid phasesyntheses *** crystalline structure and surface morphology of these ceramics are tested by x-ray diffraction and scanning electron *** green,red,and near-infrared upconversion photoluminescence properties are analyzed by the steady-state spectra under 975 nm and 800 nm *** ceramics arewell sintered and tetragonal tungsten type crystalline structure with the 4 mm point group and the P4bm space *** Er3+upconversion emission integrated intensities increase with theincrease in Er3+ion concentration,while the green emissiondecreases with the increase in Yb^(3+)ion concentration under the 800 nm *** effective energy back transfer process from the Er^(3+)^(4)S_(3/2)state to Yb^(3+)ground state plays an important role in enhancing the red emission and weakening the green emission.

关键词： ceramics crystalline Potassium

The utility of endogenous glycochenodeoxycholate-3-sulfate and 4β-hydroxycholesterol to evaluate the hepatic disposition of atorvastatin in rats

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Asian Journal of Pharmaceutical Sciences 2021年第4期16卷 519-529页

作者： Yanrong Ma Mingyan Xin Yuanjie wen huan Wang Guoqiang Zhang Jianye Dai Xin-An wu School of Pharmacy Lanzhou UniversityLanzhou 730000China Department of Pharmacy the First Hospital of Lanzhou UniversityLanzhou 730000China

The liver is an important organ for drugs disposition,and thus how to accurately evaluate hepatic clearance is essential for proper drug ***,there are many limitations in drug dosage adjustment based on liver function and pharmacogenomic *** this study,we evaluated the ability of endogenous glycochenodeoxycholate-3-sulfate(GCDCA-S)and 4β-hydroxycholesterol(4β-HC)plasma levels to evaluate organic anion-transporting polypeptide(Oatps)-mediated hepatic uptake and Cyp3 a-meidated metabolism of atorvastatin(ATV)in *** concentration of ATV and its metabolites,2-OH ATV and 4-OH ATV,was markedly increased after a single injection of rifampicin(RIF),an inhibitor of ***,plasma GCDCA-S levels were also *** a single injection of the Cyp3 a inhibitor ketoconazole(KTZ),plasma ATV concentrations were significantly increased and 2-OH ATV concentrations were decreased,consistent with the metabolism of ATV by Cyp3 ***,plasma 4β-HC was not affected by KTZ treatment despite it being a Cyp3 a metabolite of *** repeated oral administration of RIF,plasma concentrations of ATV,2-OH ATV and 4-OH ATV were markedly increased and the hepatic uptake ratio of ATV and GCDCA-S was *** did not affect plasma concentrations of ATV,2-OH ATV and 4-OH ATV,but significantly decreased the metabolic ratio of total and 4-OH ***,the plasma level and hepatic metabolism of 4β-HC were not changed by *** inhibition of hepatic uptake of GCDCA-S by RIF was fully reversed after a 7-d washout of *** concentration and hepatic uptake ratio of GCDCA-S were correlated with the plasma level and hepatic uptake of ATV in rats with ANIT-induced liver injury,*** results demonstrate that plasma GCDCA-S is a sensitive probe for the assessment of Oatps-mediated hepatic uptake of ***,Cyp3 a-mediated metabolism of ATV was not predicted by plasma 4β-HC levels in rats.

关键词： Atorvastatin Hepatic disposition Glycochenodeoxycholate-3-sulfate 4β-hydroxycholesterol Oatp Cyp3a

Evaluation of groundwater potential and eco-geological environment quality in Sanjiang Plain of Heilongjiang Province

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Journal of Groundwater Science and Engineering 2017年第2期5卷 193-201页

作者： wu Ting-wen WANG Li-huan YANG Xiang-kui School of Water Resources and Environment China University of Geosciences(Beijing) Beijing 100083 China Geological environment monitoring station in Heilongjiang Province Harbin 150000 China Heilongjiang Institute of Geological survey Harbin 150001 China

Sanjiang Plain of Heilongjiang Province has become an important commodity grain base in our country after the construction of more than a century. Groundwater is the main water source for industrial and agricultural production and daily life in the area. With the large-scale development and construction in this area, there are a series of ecological and environmental geological problems, such as the reduction of groundwater resources, which seriously restricts the sustainable development of local agriculture and society as well as economy. Based on the characteristics of three-dimensional flowof groundwater in Sanjiang Plain, this paper adopts the simulation software Visual MODFLOW to evaluate groundwater exploitation potential. The results show that overall there is a certain exploitation potential of Sanjiang Plain groundwater but it is unevenly distributed, and overdraft phenomenon exists in seven farms such as Baoquanling and Chuangye. Based on analytic hierarchy process, the evaluation result of eco-geological environment quality in Sanjiang Plain shows that 94.5% of the region features good geological environment quality, medium stability and medium bearing capacity. The study can provide geological evidence for optimal allocation of water resources, land planning and regulation, and the high and stable yield of the commodity grain base in Sanjiang Plain.

关键词： Sanjiang Plain Groundwater resources potential Eco-geological environment evaluation

GmPHR1, a Novel Homolog of the AtPHR1 Transcription Factor, Plays a Role in Plant Tolerance to Phosphate Starvation

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Journal of Integrative Agriculture 2014年第12期13卷 2584-2593页

作者： LI Xi-huan WANG Yun-jie wu Bing KONG You-bin LI wen-long CHANG wen-suo ZHANG Cai-ying North China Key Laboratory for Germplasm Resources Ministry of Education/Agricultural University of Hebei

GmPHR1 from soybean （Glycine max） was isolated and characterized. This novel homolog of the AtPHR1 transcription factor confers tolerance to inorganic phosphate （Pi）-starvation. The gene is 2 751 bp long, with an 819-bp open reading frame and ifve introns. Analysis of transcription activity in yeast revealed that the full-length GmPHR1 and its C-terminal activate the reporter genes for His, Ade and Ura, suggesting that the C-terminal peptide functions as a transcriptional activator. Quantitative real-time PCR indicated that patterns of GmPHR1 expression differed. For example, under low-Pi stress, this gene was quickly induced in the tolerant JD11 after 0.5 h, with expression then decreasing slowly before peaking at 12-24 h. By contrast, induction in the sensitive Niumaohuang （NMH） was slow, peaking at 6 h before decreasing quickly at 9 h. GmPHR1 showed sub-cellular localization in the nuclei of onion epidermal cells and Arabidopsis roots. Growth parameters in wild-type （WT） Arabidopsis plants as well as in overexpression （OE） transgenic lines were examined. Under low-Pi conditions, values for shoot, root and whole-plant dry weights, root to shoot ratios, and lengths of primary roots were signiifcantly greater in OE lines than in the WT. These data demonstrate that GmPHR1 has an important role in conferring tolerance to phosphate starvation.

关键词： GmPHR1 transcription factor AtPHR1 phosphate starvation low phosphate-stress tolerance soybean