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Moxibustion activates mast cell degranulation at the ST25 in rats with colitis

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World Journal of Gastroenterology 2011年第32期17卷 3733-3738页

作者： Yin Shi Li Qi Jing Wang Ming-Shu Xu Dan Zhang Lu-Yi wu huan-gan wu Shanghai Institute of Acupuncture-Moxibustion and Meridians Shanghai University of Traditional Chinese Medicine Shanghai 200030 China Research Centre of Interconnections between meridian and entrails Anhui University of Traditional Chinese Medicine Hefei 230038 Anhui Province China

AIM: To investigate the effects of moxibustion on the morphology and function of mast cells (MC) at Tianshu (ST25) in rats with trinitro-benzene-sulfonic acid (TNBS)-induced colitis. METHODS: A total of 53 male Sprague-Dawley rats were randomly divided into a normal group and experimental group. In the experimental group, a rat model of TNBS-induced colitis was established, and the rats were then randomly divided into a model group, moxi-bustion group, moxibustion plus disodium cromoglycate (M + DC) group and moxibustion plus normal saline (M+ NS) group. Rats in the moxibustion group received suspended moxibustion at bilateral ST25 for 10 min, once a day for 7 d. Rats in the M + DC and M + NS groups were pretreated with disodium cromoglycate and normal saline at bilateral ST25, respectively, and were then concurrently subjected to the same treatment as rats in the moxibustion group. The hematoxy- lin-eosin staining method was used to observe histology of the colon and the toluidine blue-improved method was used to observe mast cells at ST25 acupoint areas. RESULTS: An improvement in colonic injury in the moxibustion group was observed and the degranulation ratio of MC at ST25 acupoint was markedly higher in the moxibustion group than in the model group (45.91 ± 11.41 vs 32.58 ± 8.28, P < 0.05). After inhibition of degranulation of MC at ST25 by disodium cromoglycate, no improvement in colon tissue injury was observed. CONCLUSION: Moxibustion exerted its effect on healing impaired colonic mucosa in rats with TNBS-induced colitis by increasing the degranulation ratio of local MC, but had little effect on the morphology of MC at ST25 acupoint.

关键词： Disodium cromoglycate Colitis Mast cell Moxibustion ST25 acupoint

Effect of electro-acupuncture on substance P, its receptor and corticotropin-releasing hormone in rats with irritable bowel syndrome

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World Journal of Gastroenterology 2009年第41期15卷 5211-5217页

作者： Xiao-Peng Ma Lin-Ying Tan Yun Yang huan-gan wu Bin Jiang Hui-Rong Liu Ling Yang Shanghai Institute of Acupuncture-Moxibustion and Meridian Shanghai 200030 China

AIM： To investigate the effect and mechanism of electro-acupuncture lEA） at ST25 and ST37 on irritable bowel syndrome （IBS） of rats. METHODS： A total of 21 male Sprague-Dawley rats were randomly divided into normal group, model group and EA group. A rat model of IBS was established by constraining the limbs and distending the colorectum of rats. Rats in EA group received bilateral EA at ST25 and ST37 with a sparse and intense waveform at a frequency of 2/50 Hz for 15 min, once a day for 7 d as a course. Rats in normal and model groups were stimulated by distending colorectum （CR）. An abdominal withdrawal reflex （AWR） scoring system was used to evaluate improvements in visceral hypersensitivity. Toluidine blue-improved method, immunohistochemistry and radioimmunoassay were used to observe mucosal mast cells （MC）, changes of substance P （SP） and substance P receptor （SPR） in colon and change of corticotropin-releasing hormone （CRH） in hypothalamus. RESULTS： The threshold of visceral sense was significantly lower in model group than in normal group,and significantly higher in EA group than in model group. The number of mucosal MC was greater in model group than in normal group and significantly smaller in EA group than in model group. The CRH level in hypothalamus of rats was significantly higher in model group than in normal group, which was remarkably decreased after electro-acupuncture treatment. The SP and SPR expression in colon of rats in model group was decreased after electro-acupuncture treatment. CONCLUSION： EA at ST25 and ST37 can decrease the number of mucosal MC and down-regulate the expression of CRH in hypothalamus, and the expression of SP and SPR in colon of rats with IBS.

关键词： Electro-acupuncture Corticotropin-releasing hormone Irritable bowel syndrome Substance P Substance P receptor

Moxibustion down-regulates colonic epithelial cell apoptosis and repairs tight junctions in rats with Crohn's disease

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World Journal of Gastroenterology 2011年第45期17卷 4960-4970页

作者： Chun-Hui Bao Lu-Yi wu Yin Shi huan-gan wu Hui-Rong Liu Rong Zhang Li-Qing Yu Jin-Hai Wang Shanghai Institute of Acupuncture-Moxibustion and Meridian Shanghai University of Traditional Chinese Medicine Shanghai 200030 China

AIM: To investigate the effects of moxibustion on down-regulation of the colonic epithelial cell apoptosis and repair of the tight junctions in rats with Crohn's disease (CD). METHODS: Sixty male Sprague-Dawley rats were randomly divided into a normal control (NC) group, a model control (MC) group, an herbs-partitioned moxibustion (HPM) group, a mild-warm moxibustion (MWM) group and a salicylazosulphapyridine (SASP) group, with 12 rats in each group. The CD model rats were treated with trinitrobenzene sulphonic acid to induce intestinal inflammation. The rats in the HPM and MWM groups were treated at the Tianshu (ST25) and Qihai (CV6) acupoints once daily for 14 d, and the SASP group was fed SASP twice daily for 14 d. No additional treatment was given to the MC and NC groups. Themicrostructure of the colonic epithelium was observed under a transmission electron microscope, the transepithelial resistance was measured using a shortcircuit current, colonic epithelial cell apoptosis was determined by terminal deoxynucleotidyl transferasemediated dUTP-biotin nick end labelling assay, and the expression of occludin, claudin-1 and zonula occludens-l (ZO-1) in the colonic epithelial junction was determined by Western blotting and immunofluorescence staining. RESULTS: Compared with the MC group, the microstructure of the colonic epithelial barrier was signifi-cantly improved in rats treated with HPM, MWM or SASP, meanwhile, the current flow was reduced signifi-cantly, with values of 168.20 ± 6.14 vs 99.70 ± 3.13, 99.10 ± 4.28 and 120.30 ± 3.65 mA, respectively (P = 0.001). However, the HPM and MWM groups had higher current flow rates than the SASP group (99.70 ± 3.13, 99.10 ± 4.28 vs 120.30 ± 3.65 mA, P = 0.001). The number of the apoptotic colonic epithelial cells in HPM, MWM and SASP groups was largely reduced (61.5 ± 16.91 vs 15.5 ± 8.89, 14.8 ± 6.27 and 24.7 ± 9.68, respectively (P = 0.001); and the expression of occlu- din, claudin-1 and ZO-1 in the MWM and HPM groups w

关键词： Moxibustion Colonic epithelial cells apoptosis Tight junctions Colonic epithelial barrier Crohn’s disease Rats

Acupuncture and moxibustion for irritable bowel syndrome:An umbrella systematic review

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Journal of Integrative Medicine 2024年第1期22卷 22-31页

作者： Yue-ying Ma Zhou Hao Zi-yi Chen Yan-xi Shen Hui-rong Liu huan-gan wu Chun-hui Bao Shanghai Clinical Research Centre for Acupuncture and Moxibustion Yueyang Hospital of Integrated Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghai 200437China Key Laboratory of Acupuncture and Immunological Effects Shanghai University of Traditional Chinese MedicineShanghai 200030China

BackgroundIrritable bowel syndrome(IBS)is a functional bowel disease characterized by abdominal pain or discomfort associated with altered bowel *** clinical studies have demonstrated the effectiveness of acupuncture and moxibustion for *** systematic reviews of acupuncture and moxibustion for IBS have been published in recent years,but their results are not entirely *** evaluate the methodological,reporting,and evidence quality of systematic reviews of acupuncture and moxibustion for *** strategySystematic reviews of acupuncture and moxibustion for IBS published before February 20,2023 were searched in eight databases:PubMed,Embase,Cochrane Library,Web of Science,China National Knowledge Infrastructure,Wanfang Data,VIP Database for Chinese Technical Periodicals,and China Biology *** keywords used for literature search were acupuncture,moxibustion,systematic review,meta-analysis,and irritable bowel *** criteriaSystematic reviews and meta-analyses of randomized controlled trials of acupuncture and moxibustion for IBS were *** extraction and analysisRelevant information was independently extracted by two *** A MeaSurement Tool to Assess systematic Reviews 2(AMSTAR 2),Preferred Reporting Items for Systematic Reviews and Meta-Analyses(PRISMA 2020),and Grading of Recommendations Assessment,Development and Evaluation(GRADE)were used to evaluate the methodological quality,reporting quality and evidence quality,*** total of 342 studies were retrieved and 15 systematic reviews were *** results of AMSTAR 2 showed low methodological quality in 2 studies and very low methodological quality in the remaining 13 studies,with main issues being failure to register a protocol,incomplete search strategy,not providing a list of excluded studies,incomplete consideration of the risk of bias in the included studies,and a failure to assess the publication *** results of

关键词： Irritable bowel syndrome Acupuncture Moxibustion Umbrella systematic review Systematic review

Effects of moxibustion on dynorphin and endomorphin in rats with chronic visceral hyperalgesia

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World Journal of Gastroenterology 2010年第32期16卷 4079-4083页

作者： Hui-Rong Liu Li Qi Lu-Yi wu Xiao-Peng Ma Xiu-Di Qin Wen-Yan huang Ming Dong huan-gan wu Key Laboratory of Acupuncture-Moxibustion and Immunological Effects Shanghai University of Traditional Chinese MedicineShanghai 200030China

AIM:To observe the analgesic effects of moxibustion in rats with chronic visceral hyperalgesia and its influence on the concentration of dynorphin(Dyn) and endomorphin(EM) in spinal ***:The rat model of chronic visceral hyperalgesia was established by colorectal distention(CRD).In moxibustion(MX) group,moxibustion was applied once daily for 7 d;in sham moxibustion(SM) group,moxibustion was given to the same acupoints but with the nonsmoldered end of the moxa *** control(MC) group and normal control group were also *** scoring system of abdominal withdrawal reflex was used to evaluate visceral pain for behavioral *** linked immunosorbent assay was performed to determine the concentrations of Dyn and EM in spinal ***:Moxibustion significantly decreased visceral pain to CRD in this rat model,and no significant difference was detected between the SM group and the MC *** MX group,moxibustion also increased the concentrations of Dyn and EM in spinal cord,and no significant difference was found between the SM group and the MC ***:Moxibustion therapy can significantly enhance the pain threshold of rats with chronic visceral hyperalgesia,and the effect may be closely related to the increased concentration of Dyn and EM in spinal cord.

关键词： Moxibustion Analgesia Hypersensitivity Dynorphins Endomorphin

Suspended moxibustion relieves chronic visceral hyperalgesia and decreases hypothalamic corticotropin-releasing hormone levels

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World Journal of Gastroenterology 2011年第5期17卷 662-665页

作者： En-Hua Zhou Xiao-Mei Wang Guang-Hong Ding huan-gan wu Li Qi Hui-Rong Liu Shu-Jing Zhang Department of Mechanicsand Engineering Science Fudan University Shanghai 200433China Key Laboratory of Acupuncture-Moxibustion and Immunological Effects Shanghai Universityof Traditional Chinese Medicine Shanghai 201203 China Shanghai Research Center of Acupunctureand Meridians Shanghai 200433 China

AIM:To evaluate the effect of suspended moxibustion(SM) on rectal sensory thresholds and to analyze the possible mechanisms involved in SM treatment of chronic visceral hypersensitivity(CVH) in ***:SM was administered once daily to 37-dold CVH rats for 7 *** two pairs of acupoints(ST25 and ST37,bilateral) were simultaneously treated with *** treatment lasted for 30 *** undergoing treatment with SM were not *** CVH rats and normal rats were used as *** abdominal withdrawal reflex was determined 30-90 min after the seven *** hypothalamic corticotropin-releasing hormone(CRH) mRNA level was measured using real-time quantitative reverse transcriptionpolymerase chain ***:We found that SM treatment significantly decreased visceral sensitivity to colorectal distention in this rat *** treated animals,SM also decreased the relative hypothalamic CRH mRNA expression level to control ***:Lower hypothalamic CRH levels may mediate the beneficial effects of SM in this rat irritable bowel syndrome model.

关键词： Chronic visceral hypersensitivity Corticotro-pin-releasing hormone Irritable bowel syndrome Rat Suspended moxibustion

Acupuncture-moxibustion in treating irritable bowel syndrome: How does it work?

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World Journal of Gastroenterology 2014年第20期20卷 6044-6054页

作者： Xiao-Peng Ma Jue Hong Cai-Ping An Dan Zhang Yan huang huan-gan wu Cui-Hong Zhang Sian Meeuwsen Key Laboratory of Acupuncture-moxibustion and Immunology Shanghai Research Institute of Acupuncture and Meridian Department of Acupuncture and Moxibustion Obstetrics and Gynecology Hospital of Fudan University Graduate School Shanghai University of Traditional Chinese Medicine Research Department Bodystat Ltd. Douglas IM99 1DQ United Kingdom

Irritable bowel syndrome (IBS) is a functional intestinal disease characterized by abdominal pain or discomfort and altered bowel habits. It has drawn great attention because of its high prevalence, reoccurring symptoms, and severe influence on patients’ lives. Many clinical studies have demonstrated the efficacy of acupuncture-moxibustion in treating IBS. Increasing attention has been paid to research regarding the action mechanisms of acupuncture-moxibustion for IBS, and the adoption of modern techniques has achieved some progress. This article reviews the latest advances among action mechanism studies from the perspectives of gastrointestinal motility, visceral hypersensitivity, the brain-gut axis, the neuroendocrine system, and the immune system. It is shown that acupuncture-moxibustion can effectively regulate the above items, and thus, this treatment should have a high efficacy in the treatment of IBS. This article also identifies existing problems in current mechanism research and raises several ideas for future studies. Further revelations regarding these action mechanisms will promote the application of acupuncture-moxibustion in treating IBS.

关键词： Irritable bowel syndrome Acupuncture-moxibustion Mechanism study Gastrointestinal motility Visceral hypersensitivity Brain-gut axis

Effects of electroacupuncture on corticotropin-releasing hormone in rats with chronic visceral hypersensitivity

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World Journal of Gastroenterology 2015年第23期21卷 7181-7190页

作者： Hui-Rong Liu Xiao-Yi Fang huan-gan wu Lu-Yi wu Jing Li Zhi-Jun Weng Xin-Xin Guo Yu-Guang Li First Affiliated Hospital of Shantou University Medical College Shanghai Research Institute of Acupuncture and Meridian Shanghai University of Traditional Chinese Medicine

AIM: To investigate the effect of electroacupuncture on corticotropin-releasing hormone(CRH) in the colon, spinal cord, and hypothalamus of rats with chronic visceral ***: A rat model of chronic visceral hypersensitivity was generated according to the internationally accepted method of colorectal balloon dilatation. In the 7th week after the procedure, rats were randomly divided into a model group(MG), electroacupuncture group(EA), and sham electroacupuncture group(S-EA). After treatment, the abdominal withdrawal reflex(AWR) score was used to assess the behavioral response of visceral hyperalgesia. Immunohistochemistry(En Vision method), ELISA, and fluorescence quantitative PCR methods were applied to detect the expression of CRH protein and m RNA in the colon, spinal cord, and ***: The sensitivity of the rats to the colorectal distension stimulus applied at different strengths(20-80 mm Hg) increased with increasing stimulus strength, resulting in increasing AWR scores in each group. Compared with NG, the AWR score of MG was significantly increased(P 0.05) compared with normal rats(NG). However, the decrease in EA compared with MG rats was statistically significant(P < 0.01). The average optical density of CRH expression in the colon of the MG rats was significantly enhanced compared with NG(P < 0.05), while the average optical density of CRH expression in the EA and S-EA rats was significantly decreased compared with MG rats(P < 0.01, P < 0.05, respectively). Compared with MG rats, the CRH concentration in the spinal cord of EA rats was significantly reduced(P < 0.01), but there was no sign

关键词： Corticotropin-releasing hormone Electroacupuncture Irritable bowel syndrome Visceral pain Shangjuxu

Moxibustion combined with acupuncture increases tight junction protein expression in Crohn's disease patients

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World Journal of Gastroenterology 2015年第16期21卷 4986-4996页

作者： Hai-Xia Shang An-Qi Wang Chun-Hui Bao huan-gan wu Wei-Feng Chen Lu-Yi wu Rong Ji Ji-Meng Zhao Yin Shi Shanghai University of Traditional Chinese Medicine Shanghai Institute of Acupuncture-Moxibustion and Meridian Zhongshan Hospital affiliated to Fudan University

AIM:To investigate the effect of herb-partitioned moxibustion combined with acupuncture on the expression of intestinal epithelial tight junction(TJ) ***:Sixty patients diagnosed with mild to moderate Crohn’s disease(CD)were allocated into the herb-partitioned moxibustion combined with acupuncture(HMA)group(n=30)or the mesalazine(MESA)group(n=30)using a parallel control *** were 2 sets of acupoints used alternately for HMA *** following points were included in Set A:ST25(Tianshu),RN6(Qihai),and RN9(Shuifen)for herb-partitioned moxibustion and ST36(Zusanli),ST37(Shangjuxu),LI11(Quchi),and LI4(Hegu)for *** points for Set B included BL23(Shenshu)and BL25(Dachangshu)for herb-partitioned moxibustion and EX-B2 of T6-T1(Jiajixue)fo r *** patients received the same treatment6 times a week for 12 consecutive *** MESA group received 1 g of mesalazine enteric coated tablets4 times daily for 12 consecutive *** were stained and examined to compare the morphological and ultrastructural changes before and after the treatment *** and in situ hybridization assays were used to detect the expression of intestinal epithelial TJ proteins zonula occludens-1(ZO-1),occludin,and *** m RNA levels were also ***:After the treatment,both herb-partitioned moxibustion combined with acupuncture and mesalazine improved intestinal morphology and ultrastructure of CD patients;the patients treated with HMA showed better *** significantly increased the expression of ZO-1(P=0.000),occludin(P=0.021),and claudin-1(P=0.016).MESA significantly increased the expression of ZO-1(P=0.016)and occludin(P=0.026).However,there was no significant increase in the expression of claudin-1(P=0.935).There was no statistically significant difference between the two groups for the expression of occludin and claudin-1(P>0.05).The HMA group showed a significant improvement in ZO-1 exp

关键词： Crohn's disease Herb-partitioned moxibustion Acupuncture Intestinal epithelial cells Tight junctionproteins