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Fluorescent intracellular imaging of reactive oxygen species and pH levels moderated by a hydrogenase mimic in living cells

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Journal of Pharmaceutical Analysis 2022年第5期12卷 801-807页

作者： Xin-yuan Hu jia-Jing Li Zi-Wei Yang Jun Zhang huai-Song Wang Department of Pharmaceutical Analysis China Pharmaceutical UniversityNanjing210009China College of Food Science and Bioengineering Tianjin Agricultural UniversityTianjin300392China

The catalytic generation of H_(2) in living cells provides a method for antioxidant *** this study,an[FeFe]-hydrogenase mimic[Ru+Fe_(2)S_(2)@F127(80)]was synthesized by self-assembling polymeric pluronic F-127,catalytic[Fe_(2)S_(2)]sites,and photosensitizer Ru(bpy)_(3)^(2+).Under blue light irradiation,hydrated protons were photochemically reduced to H2,which increased the local pH in living cells(HeLa cells).The generated H2 was subsequently used as an antioxidant to decrease reactive oxygen species(ROS)levels in living cells(HEK 293T,HepG2,MCF-7,and HeLa cells).Our findings revealed that the proliferation of HEK 293T cells increased by a factor of about six times,relative to that of other cells(HepG2,MCF-7,and HeLa cells).Intracellular ROS and pH levels were then monitored using fluorescent cell *** study showed that cell imaging can be used to evaluate the ability of Ru t Fe2S2@F127 to eliminate oxidative stress and prevent ROS-related diseases.

关键词： Cell imaging [FeFe]-hydrogenase Reactive oxygen species Photocatalytic system

Surface lurking and interfacial ion release strategy for fabricating a superhydrophobic coating with scaling inhibition

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Petroleum Science 2022年第6期19卷 3068-3079页

作者： Ming-Liang Zhu Hui-Juan Qian Wei-Hao Fan Chi-jia Wang Rui-Xia yuan Qing-He Gao huai-yuan Wang College of Chemistry and Chemical Engineering Northeast Petroleum UniversityDaqing163318China College of Chemical Engineering Daqing Normal UniversityDaqing163712China

The design and manufacture of anti-scaling surface is a prospective way to prevent scaling in oil *** this work,a novel superhydrophobic Cu^(2+)-loaded and DTPMPA-modified anodized copper oxide(S-Cu^(2+)/D-ACO)coating was fabricated by modification of 1H,1H,2H,*** valid storing of scale inhibitors at the coating surface and the interfacial release of Cu^(2+)ions contribute to enhancing the anti-scaling of the S-Cu^(2+)/D-ACO *** water contact angle of the S-Cu^(2+)/D-ACO coating is 163.03°and exhibits superhydrophobicity,which makes it difficult for CaCO_(3)to deposit at the surface of the *** will steadily lurk into the inner space,and Cu^(2+)will be loaded at the interface in the form of the DTPMPA:Cu^(2+)*** the deposition of CaCO_(3),the dynamic release of DTPMPA can be realized by transferring DTPMPA:Cu^(2+)to DTPMPA:Ca^(2+).Interestingly,the released Cu^(2+)hinders the active growth of CaCO_(3).After 48 h of scaling,the mass of CaCO_(3)scale at the S-Cu^(2+)/D-ACO coating surface is only 44.1%that of the anodized copper oxide *** excellent anti-scaling performance of the S-Cu^(2+)/D-ACO coating is determined by the synergistic effect of the DTPMPA lurking and dynamic release,as well as the Cu^(2+)inhibition at the interface of superhydrophobic coating and against CaCO_(3)*** research provides a new exploration for designing and fabricating anti-scaling superhydrophobic surface for oil field development.

关键词： Copper alloy DTPMPA lurking And dynamic release Cu^(2+)loading and chelating CaCO3 deposition Scale inhibition mechanism

基于建构主义的“信息素养与检索实践”课程思政元素挖掘与实践

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大学图书情报学刊 2025年第1期43卷 79-85页

作者：袁帆李佳上海第二工业大学图书馆 201209 华东师范大学课程与教学研究所上海200062

文章基于建构主义理论探讨在“信息素养与检索实践”课程中如何融入思政元素,以期提升新时代高校学生信息素养及思想政治素养。通过案例教学与项目教学法挖掘课程思政元素,以能力和知识目标为抓手,构建思政元素融入信息素养教育的研究... 详细信息

文章基于建构主义理论探讨在“信息素养与检索实践”课程中如何融入思政元素,以期提升新时代高校学生信息素养及思想政治素养。通过案例教学与项目教学法挖掘课程思政元素,以能力和知识目标为抓手,构建思政元素融入信息素养教育的研究体系。研究表明,政治认同、家国情怀、科学精神及信息道德是培育课程思政的关键要素,是促进高校信息素养教学质量发展的核心指标。建立以创设问题情境、引导自主探究为主的课程思政实践机制,有助于增强独立思考与批判分析能力,提升学生的信息处理能力。研究为高校图书馆信息素养教育实现立德树人目标提供了教学策略和研究启示。

关键词：信息素养文献检索课程思政建构主义

Prediction of adsorption of small molecules in porous materials based on ab initio force field method

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Science China Chemistry 2008年第8期51卷 760-767页

作者： LIU LianChi FU jia SUN huai School of Chemistry and Chemical Technology Shanghai Jiao Tong University Shanghai 200240 China

Computational prediction of adsorption of small molecules in porous materials has great impact on the basic and applied research in chemical engineering and material sciences. In this work,we report an approach based on grand canonical ensemble Monte Carlo(GCMC) simulations and ab initio force fields. We calculated the adsorption curves of ammonia in ZSM-5 zeolite and hydrogen in MOF-5(a metal-organic-framework material). The predictions agree well with experimental data. Because the predictions are based on the first principle force fields,this approach can be used for the adsorption prediction of new molecules or materials without experimental data as guidance.

关键词： Porous materials adsorption ab initio force field Grand Canonical ensemble Monte Carlo

An image encryption algorithm based on improved baker transformation and chaotic S-box

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Chinese Physics B 2021年第6期30卷 216-225页

作者： Xing-yuan Wang huai-huai Sun Hao Gao School of Information Science and Technology Dalian Maritime UniversityDalian 116026China

The algorithm is an image encryption algorithm based on the improved baker transformation and chaotic substitution box(S-box). It mainly uses the initial values and parameters of a one-dimensional logistic chaotic system as an encryption key. Specifically, in the image scrambling stage, the algorithm primarily uses an improved baker transform method to process the image. In the image diffusion stage, the algorithm first uses the chaotic S-box method to process the encryption key. Secondly, an exclusive OR(XOR) operation is performed on the image and the encryption key to initially diffuse the image. Finally, the image is again diffused using the method of ortho XOR. Simulation analysis shows that the algorithm can achieve good encryption effect, simple and easy implementation, and good security. In the digital image communication transmission, it has good practical value.

关键词： image encryption improved baker transformation chaotic S-box chaotic sequence

Development of a seven-cell S-band standing-wave RF-deflecting cavity for Tsinghua Thomson scattering X-ray source

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Nuclear Science and Techniques 2021年第4期32卷 20-29页

作者： Xian-Cai Lin Hao Zha jia-Ru Shi Liu-yuan Zhou Shuang Liu jian Gao huai-Bi Chen Department of Engineering Physics Tsinghua UniversityBeijing 100084China Key Laboratory of Particle and Radiation Imaging(Tsinghua University) Ministry of EducationBeijing 100084China

A 2856-MHz,π-mode,seven-cell standingwave deflecting cavity was designed and fabricated for bunch length measurement in Tsinghua Thomson scattering X-ray source(TTX)*** cavity was installed in the TTX and provided a deflecting voltage of 4.2 MV with an input power of 2.5 *** length diagnoses of electron beams with energies up to 39 MeV have been *** this article,the RF design of the cavity using HFSS,fabrication,and RF test processes are ***-power operation with accelerated beams and calibration of the deflecting voltage are also presented.

关键词： Deflecting RF cavity Standing wave Bunchlength measurement Thomson X-ray source

Phase transition of the frustrated antiferromagntic J1-J2-J3 spin-1/2 Heisenberg model on a simple cubic lattice

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Frontiers of physics 2019年第1期14卷 137-146页

作者： Ai-yuan Hu huai-Yu Wang College of Physics and Electronic Engineering Chongqing Normal University Chongqing 401331 China Department of Physics Tsinghua University Beijing 100084 China

We have comprehensively investigated the frustrated J1-J2-J3 Heisenberg model on a simple cubic lattice. This model allows three regimes of magnetic order, viz., (π;π;π), (0;π;π) and (0;0;π), denoted as AF1, AF2, and AF3, respectively. The effects of the interplay of neighboring couplings on the model are studied in the entire temperature range. The zero temperature magnetic properties of this model are discussed utilizing the linear spin wave (LSW) theory, nonlinear spin wave (NLSW) theory, and Green’s function (GF) method. The zero temperature phase diagrams evaluated by the LSW and NLSW methods are illustrated, and are observed to exhibit different parameter boundaries. In certain regions and along the parameter boundaries, the possible phase transformations driven by the parameters are discussed. The results obtained using the LSW, NLSW, and GF methods are compared with those obtained using the series expansion (SE) method, and are observed to be in good agreement when the value of J2 is not close to the parameter boundaries. The ground state energies obtained using the LSW and NLSW methods are close to that obtained using the SE method. At finite temperatures, only the GF method is employed to evaluate the magnetic properties, and the calculated phase diagram is observed to be identical to the classical phase diagram. The results indicate that at the parameter boundaries, a temperature-driven first-order phase transition between AF1 and AF2 may occur along the boundary line. Along the AF1-AF3 and AF2-AF3 boundary lines, AF3 is less stable than AF1 and AF2. Our calculated critical temperature agrees with that obtained using Monte Carlo simulations and pseudofermion functional renormalization group scheme.

关键词： quantized spin models quantum phase transitions antiferromagnetics

Fabrication,tuning,and high-gradient testing of an X-band traveling-wave accelerating structure for VIGAS

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Nuclear Science and Techniques 2022年第8期33卷 77-88页

作者： Xian-Cai Lin Hao Zha jia-Ru Shi Qiang Gao jia-Yang Liu Liu-yuan Zhou jian Gao huai-Bi Chen Chuan-Xiang Tang Department of Engineering Physics Tsinghua UniversityBeijing 100084China Key Laboratory of Particle and Radiation Imaging(Tsinghua University) Ministry of EducationBeijing 100084China

X-band high-gradient linear accelerators are a challenging and attractive technology for compact electron linear-accelerator *** Very Compact Inverse Compton Scattering Gamma-ray Source(VIGAS)program at Tsinghua University will utilize X-band high-gradient accelerating structures to boost the electron beam from 50 to 350 MeV over a short distance.A constant-impedance traveling-wave structure consisting of 72 cells working in the 2π/3 mode was designed and fabricated for this *** tuning and detailed measurements were successfully applied to the *** 180 h of conditioning in the Tsinghua high-power test stand,the structure reached a target gradient of 80 MV/*** breakdown rate versus gradient of this structure was measured and analyzed.

关键词： Traveling-wave accelerating structure X-band high gradient Tuning method High-power test

Estimation of the Asian telecommunication technical efficiencies with panel data

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The Journal of China Universities of Posts and Telecommunications 2007年第1期14卷 111-114页

作者： YANG Yu-yong jia huai-jing School of Economics and Management Beijing University of Posts and Telecommunications Beijing 100876 China

This article used panel data and the Stochastic frontier analysis （SFA） model to analyze and compare the technical efficiencies of the telecommunication industry in 28 Asian countries from 1994 to 2003. In conclusion, the technical efficiencies of the Asian countries were found to steadily increase in the past decade. The high-income countries have the highest technical efficiency; however, income is not the only factor that affects the technical efficiency.

关键词： panel data SFA technical efficiency Asian telecommunication sectors