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基于A-BiLSTM和CNN的文本分类

计算机工程与设计 2024年第5期45卷 1428-1434页

作者：黄远戴晓红黄伟建于钧豪黄峥河北工程大学信息与电气工程学院河北邯郸056038

为解决单一神经网络不能获取准确全局文本信息的问题,提出一种基于A-BiLSTM双通道和优化CNN的文本分类模型。A-BiLSTM双通道层使用注意力机制关注对文本分类贡献值较大的部分,并用BiLSTM提取文本中上下文语义信息;A-BiLSTM双通道层中将... 详细信息

为解决单一神经网络不能获取准确全局文本信息的问题,提出一种基于A-BiLSTM双通道和优化CNN的文本分类模型。A-BiLSTM双通道层使用注意力机制关注对文本分类贡献值较大的部分,并用BiLSTM提取文本中上下文语义信息;A-BiLSTM双通道层中将两者输出的特征信息融合,得到高级语义;A-BiLSTM双通道层后,使用优化CNN的强学习能力提取关键局部特征,得到最终文本特征表示。分类器输出文本信息的类别。实验结果表明,该模型分类效果优于其它对比模型,具有良好的泛化能力。

关键词：文本分类深度学习双通道网络注意力机制双向长短时记忆网络卷积神经网络词向量模型

表达IFN-α的重组猪繁殖与呼吸综合病毒的构建及生物学特性分析

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动物医学进展 2024年第3期45卷 48-53页

作者：黄静王玉旭王豪陈樱欧阳康黄伟坚黄稳妃韦祖樟广西大学动物科学技术学院动物传染病与分子免疫学实验室广西南宁530005 广西壮族自治区兽用生物制品工程研究中心广西南宁530005 广西畜禽繁育与疾病防控重点实验室广西南宁530004 广西高校动物疫病预防与控制重点实验室广西南宁530005 广西农业职业技术大学动物科学技术系广西南宁530007

猪繁殖与呼吸综合征(PRRS)是危害我国生猪业的重要疫病之一,其病原是猪繁殖与呼吸综合征病毒(PRRSV),是一种免疫抑制性病毒。干扰素(IFN)是一类具有免疫调节功能和抗病毒作用的细胞因子。IFN-α除更直接的抗病毒作用外,还可以调节宿主... 详细信息

猪繁殖与呼吸综合征(PRRS)是危害我国生猪业的重要疫病之一,其病原是猪繁殖与呼吸综合征病毒(PRRSV),是一种免疫抑制性病毒。干扰素(IFN)是一类具有免疫调节功能和抗病毒作用的细胞因子。IFN-α除更直接的抗病毒作用外,还可以调节宿主的先天性和适应性免疫。论文构建表达IFN-α的重组PRRSV,分析重组病毒的生物学特性以及IFN-α的生物学活性。通过反向遗传操作方法将IFN-α插入到PRRSV ORF1b和ORF2a之间,重组质粒转染细胞后可以拯救出重组病毒(rGXAM-P-IFN-α)。插入到PRRSV基因组中的IFN-α可遗传稳定9代。重组病毒生长特性分析可发现rGXAM-P-IFN-α复制能力显著低于亲本病毒。rGXAM-P-IFN-α感染猪肺泡巨噬细胞(PAM)可显著上调抗病毒基因(PKR,ISG15和ISG54)mRNA表达水平,为进一步研发新型PRRSV疫苗提供参考。

关键词：猪繁殖与呼吸综合征病毒感染性克隆 α干扰素抗病毒基因

猪繁殖与呼吸综合征病毒重组N蛋白原核表达及间接ELISA方法的建立

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动物医学进展 2024年第9期45卷 1-6页

作者：覃建光姚静刘文波刘嘉琪陈国昌任同伟陈樱欧阳康黄伟坚韦祖樟广西大学动物科学技术学院动物传染病与分子免疫学实验室广西南宁530005 广西壮族自治区兽用生物制品工程研究中心广西南宁530005 广西畜禽繁育与疾病防控重点实验室广西南宁530004 广西高校动物疫病预防与控制重点实验室广西南宁530005

为制备猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)N蛋白多克隆抗体,建立检测PRRSV N蛋白抗体的间接ELISA方法,通过PCR扩增N蛋白基因,将N蛋白基因克隆至原核表达载体pET-32a(+),构建重组质粒pE... 详细信息

为制备猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)N蛋白多克隆抗体,建立检测PRRSV N蛋白抗体的间接ELISA方法,通过PCR扩增N蛋白基因,将N蛋白基因克隆至原核表达载体pET-32a(+),构建重组质粒pET-32a-N。将重组质粒转化至BL21(DE3)感受态细胞中,通过IPTG诱导N蛋白表达,将纯化后的重组N蛋白免疫小鼠,获得N蛋白多克隆抗体。将纯化后的N蛋白作为抗原,建立检测PRRSV N蛋白抗体的间接ELISA方法,并评估该方法的特异性、重复性和准确性。结果表明,表达的重组N蛋白呈可溶性,制备的N蛋白多克隆抗体ELISA效价能达到1∶2048000,IFA和Western blot结果表明制备的鼠多克隆抗体能特异性识别PRRSV N蛋白。建立的间接ELISA方法特异性良好,重复性试验变异系数均小于10%,且与同类型商品化试剂盒结果比较,符合率达到91.10%,可为PRRSV N蛋白的免疫学检测和结构功能的研究提供参考。

关键词：猪繁殖与呼吸综合征病毒 N蛋白原核表达多克隆抗体间接ELISA

Synthesis and characterization of insulin-5-Fu conjugate,enabling insulin as multi-drug carrier via dendritic approach

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Chinese Chemical Letters 2007年第3期18卷 247-250页

作者： Juan huang jian wei Wang Tao Gong Zhi Rong Zhang Key Laboratory of Drug Targeting Ministry of Education West China School of Pharmacy Sichuan University Chengdu 610041 China

To enable insulin as multi-drug carrier, we designed and synthesized dendritic linker molecule bearing three 5-fluorouracil residues at the branch ends. The new conjugate showed excellent water solubility. The stabilities under different conditions were investigated. The results showed that the conjugate was a potential prodrug to release free 5-Fu.

关键词： Insulin 5-Fluorouracil Prodrug Characterization Stability

A comparison of the spontaneous firing patterns between principal cells and fast spiking interneurons from dentate gyrus in waking guinea pigs

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Neuroscience Bulletin 2006年第1期22卷 21-28页

作者： Bo HU Li YANG wei huang jian-Feng SUI Department of Physiology Third Military Medical University Chongqing 400038 China

Objective To explore the possible mechanisms that cause the dentate gyrus （DG） neurons to play different roles in information coding. Methods In vivo extracellular single unit recording was performed on 22 waking female guinea pigs, which were positioned in a sound-attenuated recording chamber without any muscular relaxants. The spontaneous firing patterns of the DG neurons were detected and compared. Results There were two different electrophysiologi- cal populations in the DG of guinea pigs, principal cells （PCs） and fast spiking interneurons （INs）. Of the PCs, 1.3% discharged regularly, 48.1% irregularly and 50.6% in bursts ; in contrast, of the INs units, 64.1% discharged regularly, 2.6% irregularly and 33.3% in bursts. The spontaneous firing patterns of PCs were significantly different from those of INs （P 〈0.01 ）. In addition, the differences of several interspike interval （ISI） parameters also have been observed：（1） the ISI coefficients of variation of PCs （3.39 ± 3.56） were significantly higher than those of INs （1.08 ± 0.46）（P 〈0.01） ; （2） the ISI asymmetric indexes of PCs （0. 047±0. 059） were significantly lower than those of INs （0.569±0. 238）（P 〈 0.01 ）. Conclusion In the DG, the spontaneous firing patterns of PCs were significantly different from those of INs. The former were prone to fire in bursts, the latter were prone to fire regularly. The different roles in information coding between PCs and INs might be caused by their different firing patterns.

关键词： dentate gyrus principal cell intemeuron interspike interval spontaneous firing pattern

Portal vein embolization induces compensatory hypertrophy of remnant liver

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World Journal of Gastroenterology 2006年第3期12卷 408-414页

作者： Jing-Yao huang wei-Zhu Yang jian-Jun Li Na jiang Qu-Bin Zheng Department of Intcrvcntional Radiology TheAffiliated Union Hospital of Fujian Medical University Fuzhou350001 Fujian Province China

AIM： To evaluate the effectiveness and safety of different portal vein branch embolization agents in inducing compensatory hypertrophy of the remnant liver and to offer a theoretic basis for clinical portal vein branch embolization. METHODS： Forty-one adult dogs were included in the experiment and divided into four groups. Five dogs served as a control group, 12 as a gelfoam group, 12 as a coil-gelfoam group and 12 as an absolute ethanol group. Left portal vein embolization was performed in each group. The results from the embolization in each group using different embolic agents were compared. The safety of portal vein embolization （PVE） was evaluated by liver function test, computed tomography （CT） and digital subtraction angiography （DSA） of liver and portal veins. Statistical test of variance was performed to analyze the results. RESULTS： Gelfoam used for PVE was inefficient in recanalization of portal vein branch 4 wk after the procedure. The liver volume in groups of coil-gelfoam and absolute ethanol increased 25.1% and 33.18%, respectively. There was no evidence of recanalization of embolized portal vein, hepatic dysfunction, and portal hypertension in coil-gelfoam group and absolute ethanol group. CONCOUSION： Portal vein branch embolization using absolute ethanol and coil-gelfoam could induce atrophy of the embolized lobes and compensatory hypertrophy of the remnant liver. Gelfoam is an inefficient agent.

关键词： Portal vein embolization Interventional therapy Liver Dog

Micro-Pulse Lidar Measurements of Aerosol Vertical Structure over the Loess Plateau

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Atmospheric and Oceanic Science Letters 2008年第1期1卷 8-11页

作者： huang jian-Ping huang Zhong-wei BI jian-Rong ZHANG Wu ZHANG Lei College of Atmospheric Sciences Lanzhou University Lanzhou 730000 China

Knowledge of the vertical distribution of aerosols in the free troposphere is important for estimating their impact on climate. In this study, direct observations of the vertical distribution of aerosols in the free troposphere are made using surface Micro-Pulse Lidar (MPL) measurements. The MPL measurements were made at the Loess Plateau (35.95°N, 104.1°E), which is near the major dust source regions of the Taklimakan and Gobi deserts. The vertical distribution of the MPL backscattering suggested that nondust aerosols floated from ground level to an altitude of approximately 9 km around the source regions. Early morning hours are characterized by a shallow aerosol layer of a few hundred meters thick. As the day progresses, strong convective eddies transport the aero- sols vertically to more than 1500 m.

关键词： lidar aerosol vertical structure Loess Plateau

Overview of computation strategies on the dispersion analysis for explicit finite difference solution of acoustic wave equation

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Petroleum Science 2024年第4期21卷 2311-2328页

作者： jian-Ping huang wei-Ting Peng Ji-Dong Yang Lu-Feng Lou School of Geosciences China University of Petroleum (East China) Pilot National Laboratory for Marine Science and Technology (Qingdao)

Finite-difference(FD) method is the most extensively employed numerical modeling technique. Nevertheless, when using the FD method to simulate the seismic wave propagation, the large spatial or temporal sampling interval can lead to dispersion errors and numerical instability. In the FD scheme, the key factor in determining both dispersion errors and stability is the selection of the FD weights. Thus,How to obtain appropriate FD weights to guarantee a stable numerical modeling process with minimum dispersion error is critical. The FD weights computation strategies can be classified into three types based on different computational ideologies, window function strategy, optimization strategy, and Taylor expansion strategy. In this paper, we provide a comprehensive overview of these three strategies by presenting their fundamental theories. We conduct a set of comparative analyses of their strengths and weaknesses through various analysis tests and numerical modelings. According to these comparisons, we provide two potential research directions of this field: Firstly, the development of a computational strategy for FD weights that enhances stability; Secondly, obtaining FD weights that exhibit a wide bandwidth while minimizing dispersion errors.

关键词： Finite-difference scheme FD coefficients Dispersion error Forward modeling Numerical simulation

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锡矿山锑矿田谭家冲矿区控矿条件及找矿前景

南方金属 2024年第3期 8-12页

作者：黄建伟唐代文韦俊杰赵志坚薛超刘伍安邹利群曹毅湖南省国土空间调查监测所湖南长沙410129 锡矿山闪星锑业有限责任公司湖南冷水江417500

锡矿山锑矿田位于湘中冷水江市,是世界级的超大型锑矿,享有“世界锑都”的美誉。矿山开采已有一百多年,保有资源量逐年减少,为矿山生产的延续寻找接替资源迫在眉睫。谭家冲矿区为探矿普查区,位于锡矿山锑矿田的西北部,通过近年来对矿区... 详细信息

锡矿山锑矿田位于湘中冷水江市,是世界级的超大型锑矿,享有“世界锑都”的美誉。矿山开采已有一百多年,保有资源量逐年减少,为矿山生产的延续寻找接替资源迫在眉睫。谭家冲矿区为探矿普查区,位于锡矿山锑矿田的西北部,通过近年来对矿区的地质调查,从区域地质背景、矿区地质特征、控矿条件等,结合钻探工程分析,对谭家冲矿区找矿前景进行了预测,认为谭家冲矿区找矿前景良好。

关键词：锡矿山锑矿田谭家冲矿区控矿条件找矿前景