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Agrobacterium-Mediated Transfer of Arabidopsis ICE1 Gene into Lemon (Citrus Limon (L.) Burm. F. cv. Eureka)

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Agricultural Sciences in China 2005年第9期4卷 714-720页

作者： huang Jia-quan SUN zhong-hai Citrus Research Institute Key Laboratory of Horticultural Plant Biology Ministry of Education Huazhong Agricultural UniversityWuhan 430070 P.RChina

The Arabidopsis ICEI （inducer of CBF expression 1） gene was cloned through RT-PCR of Arabidopsis cDNAs and introduced into the lemon （Citrus Limon （L.） Burm. F. cv. Eureka） genome using Agrobacterium-mediated transformation method. Epicotyl segments from in vitro grown lemon seedlings were co-cultivated with A. tumefaciens strain EHA 105 carrying the binary plasmid pMVICE1, whose T-DNA region contain ICEI gene driven by 35S CaMV promoter. Among 320 epicotyl segments inoculated, 71 explants responded and regenerated 51 elongated shoots. These shoots were subjected to an extra month of kanamycin exposure. In this way, the number of escapes reduced. Thirteen of 31 survived shoots formed roots and 7 were tested positive using PCR technique. Southern blot analyses confirmed PCR results and demonstrated that more than two copies of the ICE1 gene were integrated into the lemon genome.

关键词： Lemon ICE1 Agrobacterium-mediated transformation Regeneration

Enantioselective reductive conjugate alkenylation of α,β-enones with keto alkenyl acetates by nickel catalysis

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Science China Chemistry 2025年第1期68卷 157-162页

作者： Rong-De He Yun-Lei Luo Qiu-quan Pan Qi-Wei Yao Xing-zhong Shu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical Engineering Lanzhou University

Reactions involving alkenyl acetates offer cost-effective and environmentally friendly routes for alkene synthesis, yet their asymmetric variant remains elusive. Concurrently, asymmetric conjugate alkenylation predominantly centered on nucleophilic addition using alkenyl–M. This manuscript presents an asymmetric reductive conjugate alkenylation reaction involving alkenyl acetates. The method facilitates the enantioselective addition of keto alkenyl groups to α,β-enones, resulting in the formation of unsaturated diketones—a class of useful structural motifs that are challenging to access otherwise. The use of electron-rich Pyroxy ligand is essential for achieving both high reaction efficiency and enantioselectivity.

关键词： nickel alkenylation asymmetric cross-coupling alkenes

基于XML的海洋环境基础数据格式转换的研究

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海洋通报 2017年第6期36卷 700-705页

作者：温玉波黄全中国家海洋局南海信息中心广东广州510310

针对当前海洋科学研究、海洋预报、海洋环境保护等工作中遇到的海洋环境基础数据格式不一致的问题,通过对数据语义、语法、结构的分析,建立数据之间格式转换模型,在此基础上提出基于可扩展标记语言(XML)转换模板的数据格式转换体系,并... 详细信息

针对当前海洋科学研究、海洋预报、海洋环境保护等工作中遇到的海洋环境基础数据格式不一致的问题,通过对数据语义、语法、结构的分析,建立数据之间格式转换模型,在此基础上提出基于可扩展标记语言(XML)转换模板的数据格式转换体系,并以台站观测温盐数据为例,设计编写程序成功实现数据间的格式转换。

关键词：海洋环境数据格式转换元数据映射可扩展标记语言(XML) 数据建模

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交易情景下专利价值影响因素分析

科学学研究 2020年第9期38卷 1608-1620页

作者：钱坤张晓黄忠全南京财经大学会计学院江苏南京210023 江苏省人民医院资产处江苏南京210029 江苏五星资产评估有限责任公司江苏南京210008

从专利交易情景的角度出发,分析专利价值的影响因素,对提升专利交易市场的效率和活性具有积极的现实意义。文章以2009-2016年江苏省技术交易合同整理获得的463份专利交易合同为基础,运用统计软件对手工搜集到的491条专利样本数据对专利... 详细信息

从专利交易情景的角度出发,分析专利价值的影响因素,对提升专利交易市场的效率和活性具有积极的现实意义。文章以2009-2016年江苏省技术交易合同整理获得的463份专利交易合同为基础,运用统计软件对手工搜集到的491条专利样本数据对专利价值的影响因素进行实证分析,结果表明,高校专利的价值显著低于企业专利的价值,且依据专利权人类型的不同,专利属性对专利价值的影响存在差异性。专利国际分类数和专利维持时间对高校专利价值具有显著正向影响,而企业专利价值受专利权利要求数的影响较为显著。此外,交易合同中的附条件支付条款可以显著增加高校专利的价值,但对企业专利价值的影响并不显著。

关键词：专利交易专利价值影响因素专利属性实证分析

Cobalamin-dependent radical S-adenosyl-L-methionine protein functions with a partner to successively methylate tricyclic indole alkaloid for chuangxinmycin maturation and derivatization

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Science China Chemistry 2025年第1期68卷 308-316页

作者： Sili Wang Jiancheng huang Yanan Du Wei huang Yufeng Xue Xiaokun Xu Guannan zhong Yuanyuan Shi Bin Hong Xiaoying Bian Wen Liu State Key Laboratory of Chemical Biology Shanghai Institute of Organic Chemistry University of Chinese Academy of SciencesChinese Academy of Sciences State Key Laboratory of Microbial Technology Shandong University National Health Commission (NHC) Key Laboratory of Biotechnology for Microbial Drugs Institute of Medicinal BiotechnologyChinese Academy of Medical Science & Peking Union Medical College

Cobalamin(Cbl)-dependent radical S-adenosyl-L-methionine(SAM) proteins constitute the largest collection of the radical SAM superfamily that has hundreds of thousands of individual members. Many of these proteins are involved in the biosynthesis of pharmaceutically important natural products to catalyze chemically demanding reactions. In the biosynthetic pathway of chuangxinmycin(CXM), a unique indole alkaloid antibiotic with potent anti-infective activity, functionalization of the characteristic thiopyrano[4,3,2-cd]indole scaffold by regio-and stereoselective C3-methylation is believed to rely on a Cbl-dependent radical process, which, however, remained to be reconstituted biochemically. We here report the dissection of this enzymatic process, which requires the incorporation of Cxm8, a Cbl-dependent radical SAM protein, with Cxm9, a DUF5825family protein that shares no homology to any proteins of known functions. Cxm8 and Cxm9 function together by forming an unexpected heterodimeric complex that selectively catalyzes C3-methylation of the tricyclic indole-S-hetero ring system in a successive manner, achieving CXM and a recently identified, C3-dimethylated congener. Detailed biochemical characterization,isotope labeling, structural simulation and bioinformatics analysis rationalized the catalysis of the Cxm8/Cxm9 complex and particularly the necessity of the DUF5825 protein for C3-methylase activity. This is the first example that a Cbl-dependent protein acts with a partner to exhibit radical SAM activity.

关键词： cbl-dependent radical SAM protein biosynthesis chuangxinmycin stereoselective methylation

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基于专利质押的P2P网贷信号博弈分析

软科学 2018年第6期32卷 108-112,118页

作者：钱坤潘玥黄忠全南京财经大学会计学院南京210023 江苏五星资产评估有限责任公司南京210008

针对互联网金融与专利质押结合的融资新模式,构建了借款企业与投资人之间信号博弈模型,分析了参与双方的策略选择以及均衡状态的影响因素。研究表明：考虑信息传递的动态性和不完全性,专利质押物可变现价值和信息掩饰成本是影响互联网... 详细信息

针对互联网金融与专利质押结合的融资新模式,构建了借款企业与投资人之间信号博弈模型,分析了参与双方的策略选择以及均衡状态的影响因素。研究表明：考虑信息传递的动态性和不完全性,专利质押物可变现价值和信息掩饰成本是影响互联网金融市场均衡的关键因素。因此,提高P2P平台专利质押融资效率应通过加快知识产权交易平台建设,引入互联网融资征信机制,加强P2P平台融资项目的内部监管以及完善P2P平台信息披露机制等途径来实现。

关键词： P2P融资信息不对称专利质押信号博弈

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基于演化博弈视角的邻避设施环境补偿机理

系统工程 2017年第3期35卷 88-94页

作者：钱坤黄忠全刘小峰南京财经大学会计学院江苏南京210023 南京大学工程管理学院江苏南京210009

近年来,环境补偿作为缓解邻避行为的重要经济政策逐渐成为研究热点,文章基于有限理性和不完全信息视角,构建邻避设施建设中针对环境补偿问题的社区民众和地方政府之间演化博弈模型,分析了参与双方的演化稳定策略,考察模型参量对参与双... 详细信息

近年来,环境补偿作为缓解邻避行为的重要经济政策逐渐成为研究热点,文章基于有限理性和不完全信息视角,构建邻避设施建设中针对环境补偿问题的社区民众和地方政府之间演化博弈模型,分析了参与双方的演化稳定策略,考察模型参量对参与双方演化博弈均衡状态的影响。研究表明:在对邻避设施周围社区民众进行环境补偿的同时,努力降低社区民众通过不合作策略改变邻避设施环境负效应的预期才是缓解邻避冲突问题的关键,要促成社区民众和地方政府之间协调,需要实现参与式公共政策设计,适时公开邻避设施的建设方案和环境影响评价,而法律监督机制的完善和第三方环境损失评估机构的引入也是缓解邻避冲突的重要途径。

关键词：邻避设施环境补偿演化博弈有限理性

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非开挖水平定向钻进软件设计研究

煤田地质与勘探 2005年第1期33卷 76-78页

作者：周从明黄中权宁波市机电工业研究设计院浙江宁波315012 成都勘测设计研究院四川成都610000

轨迹设计是非开挖水平定向钻进管线铺设施工的关键。本文应用动态规划原理 ,对轨迹最优化设计进行了深入研究。根据工程实际需要 ,设计出一套水平定向钻进软件 ,实现了轨迹最优化设计、施工图设计、竣工图处理、施工记录表填写及轨迹查... 详细信息

轨迹设计是非开挖水平定向钻进管线铺设施工的关键。本文应用动态规划原理 ,对轨迹最优化设计进行了深入研究。根据工程实际需要 ,设计出一套水平定向钻进软件 ,实现了轨迹最优化设计、施工图设计、竣工图处理、施工记录表填写及轨迹查询等功能 ,集设计、优化、出图于一体。实践证明。

关键词：水平定向钻进轨迹最优化软件设计

A promising approach for quantifying focal stroke modeling and assessing stroke progression:optical resolution photoacoustic microscopy photothrombosis

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Neural Regeneration Research 2025年第7期20卷 2029-2037页

作者： Xiao Liang Xingping quan Xiaorui Geng Yujing huang Yonghua Zhao Lei Xi Zhen Yuan Ping Wang Bin Liu Faculty of Health Sciences University of MacaoMacao Special Administrative RegionChina Center for Cognitive and Brain Sciences University of MacaoMacao Special Administrative RegionChina Department of Biomedical Engineering Southern University of Science and TechnologyShenzhenGuangdong ProvinceChina State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoTaipaMacao Special Administrative RegionChina Department of Emergency Zhujiang HospitalSouthern Medical UniversityGuangzhouGuangdong ProvinceChina

To investigate the mechanisms underlying the onset and progression of ischemic stroke,some methods have been proposed that can simultaneously monitor and create embolisms in the animal cerebral ***,these methods often require complex systems and the effect of age on cerebral embolism has not been adequately studied,although ischemic stroke is strongly *** this study,we propose an optical-resolution photoacoustic microscopy-based visualized photothrombosis methodology to create and monitor ischemic stroke in mice simultaneously using a 532 nm pulsed *** observed the molding process in mice of different ages and presented age-dependent vascular embolism ***,we integrated optical coherence tomography angiography to investigate age-associated trends in cerebrovascular variability following a *** imaging data and quantitative analyses underscore the differential cerebrovascular responses to stroke in mice of different ages,thereby highlighting the technique's potential for evaluating cerebrovascular health and unraveling age-related mechanisms involved in ischemic strokes.

关键词： age-dependent cerebral cortex ischemic stroke mouse model optical coherence tomography angiography photoacoustic microscopy photothrombosis vascular imaging