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First wetland mapping at 10-m spatial resolution in South America using multi-source and multi-feature remote sensing data

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Science China Earth Sciences 2024年第10期67卷 3252-3269页

作者： Weiwei SUN Gang YANG Yuling huang Dehua MAO ke huang Lin ZHU Xiangchao MENG Tian FENG Chao CHEN Yong GE Department of Geography and Spatial Information Techniques Ningbo University State key Laboratory of Resources and Environmental Information System Institute of Geographic Sciences & Natural Resources ResearchChinese Academy of Sciences Natural Resources and Planning Bureau Shanghai Pudong New District key Laboratory of Wetland Ecology and Environment Northeast Institute of Geography and Agroecology Chinese Academy of Sciences Faculty of Electrical Engineering and Computer Science Ningbo University School of Geography Science and Geomatics Engineering Suzhou University of Science and Technology

Wetland degradation has been accelerating in recent years globally. Accurate information on the geographic distribution and categories of wetlands is essential for their conservation and management. Despite being the world′s fourth largest continent, South America has limited research on wetland mapping, and there is currently no available map that provides comprehensive information on wetland distribution and categories in the region. To address this issue, we used Sentinel-1, Sentinel-2 and SRTM data, developed a sample collection method and a wetland mapping method with a collection of multi-source features such as optical features, polarization features and shape features for South American wetlands. We produced a 10-m resolution wetland map based on the Google Earth Engine（GEE） platform. Our Level-1 wetland cover map accurately captured six wetland sub-categories with an overall accuracy of 96.24% and a kappa coefficient of 0.8649, while our Level-2 water cover map included five sub-categories with an overall accuracy of 97.23% and a kappa coefficient of0.9368. The results show that the total area of existing wetlands in South America is approximately 1,737,000 km2, which is6.8% of the total land area. Among the ten wetland categories, shallow sea had the largest area（960,527.4 km2）, while aquaculture ponds had the smallest area 1513.6 km2. Swamp had the second largest area（306,240.1 km2）. Brazil, Argentina,Venezuela, Bolivia, and Colombia were found to have the largest wetland areas, with Brazil and Colombia having the most diverse wetland categories. This product can serve as baseline data for subsequent monitoring, management, and conservation of South American wetlands.

关键词： Wetland mapping Google Earth Engine Sentinel imagery South America

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Microstructure Recrystallization and Mechanical Properties of a Cold-Rolled TiNbZrTaHf Refractory High-Entropy Alloy

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Acta Metallurgica Sinica(English Letters) 2024年第4期37卷 633-647页

作者： Chuan Rong Jieren Yang Xiaoliang Zhao ke huang Ying Liu Xiaohong Wang Dongdong Zhu Ruirun Chen College of Materials Science and Engineering Sichuan UniversityChengdu610065China key Laboratory of Air-Driven Equipment Technology of Zhejiang Province Quzhou UniversityQuzhou324000China School of Materials Science and Engineering Harbin Institute of TechnologyHarbin150001China

The equiatomic TiNbZrTaHf alloy was successfully rolled at room temperature with the reduction of ~ 85%. The microstructure and tensile properties were investigated after cold working and annealing. It was determined that the recrystallization temperature of the TiNbZrTaHf alloy between 850 and 900 ℃. Complete recrystallization and normal grain growth occurred, the high stability of single phase was maintained after annealing at 1000, 1200, and 1400 ℃. But the precipitated phase appeared after long term annealing, as seen after 500 h at 1000 ℃. After cold working, the tensile strength and the elongation of TiNbZrTaHf were 1137 MPa and 25.1%, respectively. The annealed alloy has a high tensile strength (σ_(b )= 863 MPa) and ductility (ε_(e )= 28.5%). Moreover, the oxidation of TiNbZrTaHf alloy at elevated temperatures has a significant impact on its mechanical properties. The poor oxidation resistance of TiNbZrTaHf can accelerate tensile failure by inducing fractures at grain boundaries.

关键词： Refractory high entropy alloy Cold rolling Recrystallization Oxidation resistance Mechanical properties

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Selenocysteine methyltransferase SMT catalyzed the synthesis of Se-methylselenocysteine to regulate the accumulation of glucosinolates and sulforaphane in broccoli

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Horticultural Plant Journal 2024年第5期10卷 1151-1164页

作者： Qi Wu Junwei Wang Yuxiao Tian Chunyan Zhou Shuxiang Mao Qiuyun Wu ke huang College of Horticulture Hunan Agricultural UniversityChangshaHunan 410128China Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding Ministry of EducationChangshaHunan 410128China key Laboratory for Vegetable Biology of Hunan Province ChangshaHunan 410128China

Selenocysteine methyltransferase(SMT)is a key enzyme involved in the Se metabolism pathway,and it is responsible for the catalysis of Se-methylselenocysteine(SeMSC)compound *** studies showed that selenium treatment activated SMT expression and promoted the accumulation of glucosinolates(GSLs)and sulforaphane,but the roles and functional mechanisms of SMT in mediating GSLs and sulforaphane synthesis remain *** this study,we identified the BoSMT gene in broccoli and uncovered its roles in mediating GSLs *** assays revealed that BoSMT is involved in SeMSC biosynthesis in *** importantly,the contents of GSLs and sulforaphane were significantly increased in the BoSMT-overexpressing broccoli lines but decreased in the knockdown lines,suggesting that BoSMT played a positive role in regulating GSLs and sulforaphane *** evidence indicated that BoSMT-mediated overaccumulation of GSLs and sulforaphane might be due to the increase in the endogenous SeMSC *** with the mock(water)treatment,selenite-induced significantly increases of the SeMSC content in the BoSMT-knockdown plants partially compensated the phenotype of GSLs and sulforaphane *** with the mock treatment,exogenous SeMSC treatment significantly increased the contents of GSL and sulforaphane and activated GSL synthesis-related gene expression,suggesting that SeMSC acted as a positive regulator for GSL and sulforaphane *** findings provided novel insights into selenium-mediated GSLs and sulforaphane *** genetic manipulation of BoSMT might be a useful strategy for improving the dietary nutritional values of broccoli.

关键词： Selenocysteine methyltransferase Transgenic broccoli Se-methylselenocysteine Glucosinolate sulforaphane Selenium

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BoMyrosinase plays an essential role in sulforaphane accumulation in response to selenite treatment in broccoli

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Horticultural Plant Journal 2024年第2期10卷 488-502页

作者： Qiuyun Wu Qi Wu Yuxiao Tian Chunyan Zhou Shuxiang Mao Junwei Wang ke huang College of Horticulture Hunan Agricultural UniversityChangshaHunan 410128China Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding Ministry of EducationChangshaHunan 410128China key Laboratory for Vegetable Biology of Hunan Province ChangshaHunan 410128China key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops Ministry of Agriculture and Rural Affairs of ChinaChangshaHunan 410128China

Sulforaphane, a naturally specialized metabolite, plays significant roles in human disease prevention and plant defense. Myrosinase(MY) is a key gene responsible for the catalysis of sulforaphane formation, but the molecular mechanisms through which MY regulates sulforaphane biosynthesis in plants remains largely unknown. Here, we discovered that the change of sulforaphane content in broccoli sprouts caused by exogenous selenite treatments is positively related to BoMY expression. BoMY overexpression in the Arabidopsis thaliana tgg1 mutants could dramatically increase myrosinase activity and sulforaphane content in the rosette leaves of 35S::BoMY/tgg1 and rescue its ***, an obvious increase of myrosinase activity and sulforaphane content was displayed in transgenic BoMY-overexpressed broccoli *** addition, a 2 033 bp promoter fragment of BoMY was isolated. Yeast one-hybrid(Y1H) library screening experiment uncovered that one bHLH transcription factor, BoFAMA, could directly bind to BoMY promoter to activate its expression, which was further evidenced by Y1H assay and dual-luciferase reporter assay. BoFAMA is a selenite-responsive transcription factor that is highly expressed in broccoli leaves;its protein is solely localized to nucleus. Additionally, genetic evidence suggested that the knockdown of FAMA gene in Arabidopsis thaliana could significantly decrease sulforaphane yield by inhibiting the expression of myrosinase genes. Interestingly, exogenous selenite supply could partially restore the low level of sulforaphane content in transgenic Arabidopsis FAMA-silencing plants. Our findings uncover a novel function of FAMAMY module in the regulation of selenite-mediated sulforaphane synthesis and provide a new insights into the molecular mechanism by which selenite regulates the accumulation of sulforaphane in plants.

关键词： Brassica oleracea var.italica Myrosinase BoMY Sulforaphane Selenite treatment

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Absorbed Bioactive Compounds Replicate GuanxinⅡ-Induced Endothelium-Associated in/ex vivo Vasodilation

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Chinese Journal of Integrative Medicine 2024年第5期30卷 387-397页

作者： XU Min LIU Hao SU Meng-qing LI Lan YU Ai-ling CHEN ken huang Yun-ke ZHAO Qiu-long huang Wen-ya huang Xi Institute of Traditional Chinese Medicine Related Comorbid Depression Nanjing University of Chinese MedicineNanjing 210029China Department of Gynecology Women's HospitalZhejiang University School of MedicineHangzhou 310053China

Objective:To develop an interference-free and rapid method to elucidate GuanxinⅡ(GXⅡ)'s representative vasodilator absorbed bioactive compounds(ABCs)among enormous ***:The contents of ferulic acid,tanshinol,and hydroxysafflor yellow A(FTA)in GXⅡ/rat serum after the oral administration of GXⅡ(30 g/kg)were detected using ultra-performance liquid chromatography-mass *** 18 rats were randomly assigned to the control group(0.9%normal saline),GXⅡ(30 g/kg)and FTA(5,28 and 77 mg/kg)by random number table *** coronary flow velocity-time integral(VTI),i.e.,coronary flow or coronary flow-mediated dilation(CFMD),and endothelium-intact vascular tension of isolated aortic rings were *** 12 h of exposure to blank medium or 0.5 mmol/L H_(2)O_(2),endothelial cells(ECs)were treated with post-dose GXⅡof supernatant from deproteinized serum(PGSDS,300μL PGSDS per 1 m L of culture medium)or FTA(237,1539,and 1510 mg/m L)for 10 min as control,H_(2)O_(2),PGSDS and FTA *** oxide(NO),vascular endothelial growth factor(VEGF),endothelin-1(ET-1),superoxide dismutase(SOD),malondialdehyde(MDA)and phosphorylated phosphoinositide 3 kinase(p-PI3K),phosphorylated protein kinase B(p-AKT),phosphorylated endothelial nitric oxide synthase(p-e NOS)were *** was developed as a GXⅡproxy of ex vivo herbal crude ***:PGSDS effectively eliminates false responses caused by crude GXⅡ*** doses equaled the contents in GXⅡ/its post-dose serum,FTA accounted for 98.17%of GXⅡ-added CFMD and 92.99%of PGSDS-reduced vascular *** ECs,FTA/PGSDS was found to have significant antioxidant(lower MDA and higher SOD,Pkedly abolished by NG-nitro-L-arginine methyl ester(L-NA,e NOS inhibitor)and wortmannin(PI3K/AKT inhibitor

关键词： ultra-performance liquid chromatography-mass spectrometry endothelium-dependent vasodilation herbal extract GuanxinⅡ absorbed bioactive compound

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The Combined Effects of High Temperatures and Ozone Pollution on Medical Emergency Calls—Jinan City,Shandong Province,China,2013–2019

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China CDC weekly 2024年第2期6卷 30-35,I0001-I0007页

作者： Huiyun Chang Chong Liu Xingyi Geng Xiumiao Peng ke huang Liheng Wang Liangliang Cui Department of Scientific Research and Education Jinan Municipal Center for Disease Control and PreventionJinan Municipal Center for Disease Control and Prevention Affiliated to Shandong UniversityJinan CityShandong ProvinceChina Jinan Health Well Development Center Jinan CityShandong ProvinceChina Department of Environmental Health Jinan Municipal Center for Disease Control and PreventionJinan CityShandong ProvinceChina Department of Emergency Jinan Medical Emergency CenterJinan CityShandong ProvinceChina

What is already known about this topic?Studies have extensively documented the separate and independent effects of extreme temperature and ozone on morbidity and mortality associated with respiratory and circulatory *** is added by this report?The study revealed a significant association between elevated temperature,ozone pollution,and the combined effect of high temperature and ozone pollution with an increased risk of all-cause medical emergency calls(MECs)and MECs specifically related to neurological *** are the implications for public health practice?Interventional measures should be implemented to mitigate exposure to high temperatures and ozone ***,during the warm season,it is crucial for relevant authorities to focus on disseminating scientific information regarding the health impacts of elevated temperatures and ozone ***,timely public health advisories should be issued to alert the public effectively.

关键词： ozone elevated pollution

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Effect of quenching cooling rate on residual stress and microstructure evolution of 6061 aluminum alloy

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Journal of Central South University 2024年第7期31卷 2167-2180页

作者： huang ke YI You-ping huang Shi-quan HE Hai-lin LIU Jie HUA Hong-en TANG Yun-jian Research Institute of Light Alloy Central South UniversityChangsha 410083China State key Laboratory of Precision Manufacturing for Extreme Service Performance Changsha 410083China School of Mechanical and Electrical Engineering Central South UniversityChangsha 410083China

In this study,the cooling rate was manipulated by quenching with water of different temperatures(30,60 and 100℃).Surface and internal residual stresses in the quenched 6061 aluminum alloy samples were measured using hole-drilling and crack compliance methods,***,the processability of the quenched samples was evaluated at cryogenic *** mechanical properties of the as-aged samples were assessed,and microstructure evolution was *** surface residual stresses of samples W30℃,W60℃and W100℃is−178.7,−161.7 and−117.2 MPa,respectively along x-direction,respectively;and−191.2,−172.1 and−126.2 MPa,respectively along *** sample quenched in boiling water displaying the lowest residual stress(~34%and~60%reduction in the surface and core).The generation and distribution of quenching residual stress could be attributed to the lattice distortion *** plasticity was also exhibited in the samples with relatively low quenching cooling rates at cryogenic *** strengthes of the as-aged samples are 291.2 to 270.1 MPa as the quenching water temperature increase from 30℃to 100℃.Fine and homogeneous β"phases were observed in the as-aged sample quenched with boiling water due to the clusters and Guinier-Preston zones(GP zones)premature precipitated during quenching process.

关键词： 6061 aluminum alloy residual stress cooling rate cryogenic cooling mechanical properties microstructure evolution

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Rapid Antidepressant-Like Potential of Chaihu Shugan San Depends on Suppressing Glutamate Neurotransmission and Activating Synaptic Proteins in Hippocampus of Female Mice

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Chinese Journal of Integrative Medicine 2024年第8期30卷 692-700页

作者： LU Chao GAO Zi-wei XING Shan WANG Hui-hui huang Yun-ke ZHOU Hang WU Lei Department of Chinese Medicine Preparations Affiliated Hospital of Nanjing University of Chinese MedicineJiangsu Province Hospital of Chinese MedicineNanjing210029China College of Chinese Medicine Nanjing University of Chinese MedicineNanjing210046China Department of Chinese Medicine Women’s HospitalSchool of MedicineZhejiang UniversityHangzhou310000China

Objective To explore the rapid antidepressant potential and the underlying mechanism of Chaihu Shugan San(CSS)in female *** Liquid chromatography mass spectrometry(LC-MS)/MS was used to determine the content of main components in CSS to determine its *** C57BL/6J mice were randomly divided into 4 groups,including control(saline),vehicle(saline),CSS(4 g/kg)and ketamine(30 mg/kg)*** were subjected to irregular stress stimulation for 4 weeks to establish the chronic mild stress(CMS)model,then received a single administration of *** hours later,the behavioral tests were performed,including open field test,tail suspension test(TST),forced swimming test(FST),novelty suppression feeding test(NSF),and sucrose preference test(SPT).Western blot analysis was used to detect the expression levels of N-methyl-D-aspartate receptor(NMDA)subtypes[N-methyl-D-aspartate receptor 1(NR1),NR2A,NR2B],synaptic proteins[synapsin1 and post synaptic density protein 95(PSD95)],and brain-derived neurotrophic factor(BDNF).Moreover,the rapid antidepressant effect of CSS was tested by pharmacological technologies and optogenetic interventions that activated glutamate receptors,*** Compared with the vehicle group,a single administration of CSS(4 g/kg)reversed all behavioral defects in TST,FST,SPT and NSF caused by CMS(Pketamine,CSS increased levels of synaptic proteins and BDNF(Pked by transient activation of NMDA receptors in the hippocampus(all P<0.01).Conclusion Rapid antidepressant effects of CSS by improving behavioral deficits in female CMS mice depended on rapid suppression of NMDA receptors and activation of synaptic proteins.

关键词： Chaihu Shugan San female rapid antidepressant potential N-methyl-D-aspartate receptor synapticprotein optogenetic Chinesemedicine

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Revealing precipitation behavior and mechanical response of wire-arc directed energy deposited Mg-Gd-Y-Zr alloy by tailoring aging procedures

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INTERNATIONAL JOURNAL OF EXTREME MANUFACTURING 2024年第4期6卷 045001-045001页

作者： Li, Xinzhi Fang, Xuewei Zhang, Zhiyan Ghafoor, Shahid Chen, Ruikai Liu, Yi Tang, kexin Li, Kai Ma, Minghua Shang, Jiahao huang, ke Xi An Jiao Tong Univ Sch Mech Engn State Key Lab Mfg Syst Engn Xian 710049 Shaanxi Peoples R China

Mg-Gd-Y-Zr alloy, as a typical magnesium rare-earth (Mg-RE) alloy, is gaining popularity in the advanced equipment manufacturing fields owing to its noticeable age-hardening properties and high specific strength. However, it is extremely challenging to prepare wrought components with large dimensions and complex shapes because of the poor room-temperature processability of Mg-Gd-Y-Zr alloy. Herein, we report a wire-arc directed energy deposited (DED) Mg-10.45Gd-2.27Y-0.52Zr (wt.%, GW102K) alloy with high RE content presenting a prominent combination of strength and ductility, realized by tailored nanoprecipitates through an optimized heat treatment procedure. Specifically, the solution-treated sample exhibits excellent ductility with an elongation (EL) of (14.6 +/- 0.1)%, while the aging-treated sample at 200 degrees C for 58 h achieves an ultra-high ultimate tensile strength (UTS) of (371 +/- 1.5) MPa. Besides, the aging-treated sample at 250 degrees C for 16 h attains a good strength-ductility synergy with a UTS of (316 +/- 2.1) MPa and a EL of (8.5 +/- 0.1)%. Particularly, the evolution mechanisms of precipitation response induced by various aging parameters and deformation behavior caused by nanoprecipitates type were also systematically revealed. The excellent ductility resulted from coordinating localized strains facilitated by active slip activity. And the ultra-high strength should be ascribed to the dense nano-beta ' hampering dislocation motion. Additionally, the shearable nano-beta 1 contributed to the good strength-ductility synergy. This work thus offers insightful understanding into the nanoprecipitates manipulation and performance tailoring for the wire-arc DED preparation of large-sized Mg-Gd-Y-Zr components with complex geometries. The GW102K component is fabricated by wire-arc directed energy deposition. The precipitation behavior and mechanical response induced by aging parameters are clarified. The relationship among the microstructure evolution

关键词： wire-arc directed energy deposition Mg-Gd-Y-Zr alloy precipitation response prominent strength-ductility combination deformation mechanism MAGNESIUM MICROSTRUCTURE STRENGTH ORIENTATION DUCTILITY SHAPE

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Influenza and pneumococcal vaccination coverage and associated factors in patients hospitalized with acute exacerbations of COPD in China:Findings from real-world data

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Chinese Medical Journal 2024年第10期137卷 1179-1189页

作者： Ruoxi He Xiaoxia Ren ke huang Jieping Lei Hongtao Niu Wei Li Fen Dong Baicun Li Ye Wang Ting Yang Chen Wang School of Population Medicine and Public Health Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Department of Respiratory Medicine National Key Clinical SpecialtyBranch of National Clinical Research Center for Respiratory DiseaseXiangya HospitalCentral South UniversityChangshaHunan 410008China Department of Pulmonary and Critical Care Medicine Center of Respiratory MedicineChina-Japan Friendship HospitalBeijing 100029China Institute of Respiratory Medicine Chinese Academy of Medical SciencesBeijing 100029China National Center for Respiratory Medicine Beijing 100029China National Clinical Research Center for Respiratory Diseases Beijing 100029China Department of Clinical Research and Data Management Center of Respiratory MedicineChina-Japan Friendship HospitalBeijing 100029China

Background:Influenza and pneumococcal vaccination are a priority in patients with chronic obstructive pulmonary disease(COPD).However,limited information is available on vaccination coverage among patients with acute exacerbations of COPD(AECOPD)in *** study aimed to determine the rates and associated factors of influenza and pneumococcal vaccination in patients hospitalized with ***:Baseline data from a national,multicenter,hospital-based study that included adult inpatients with AECOPD between 2017 and 2021 were *** outcomes of interest were the influenza vaccination in the past year and the pneumococcal vaccination in the past 5 *** ensure national representativeness,rates were weighted according to the distribution of hospital levels and types enrolled in this *** Poisson regression based on mixed-effects models were used to determine the associated *** independent variables included the region and hospital features where the participants were located,sociodemographic characteristics(age,sex,rural/urban residence,education,etc.),and clinical indicators(COPD disease history,lung function parameters,comorbidities,etc.).The treatment profiles of the vaccinated and unvaccinated participants were ***:Of 6949 eligible participants,the weighted rates of influenza/pneumococcal,influenza,and pneumococcal vaccination were 2.72%(95%confidence interval[CI]:2.34%-3.10%),2.09%(95%CI:1.76%-2.43%),and 1.25%(95%CI:0.99%-1.51%),*** multivariable models,age≥60 years(60-69 years,odds ratio[OR]:1.90,95%CI:1.11-3.25;≥80 years,OR:2.00,95%CI:1.06-3.78),geographical regions(Northern China relative to Eastern China,OR:5.09,95%CI:1.96-13.21),urban residence(OR:1.69,95%CI:1.07-2.66),a higher education level(junior high school,OR:1.77,95%CI:1.21-2.58;senior high school or above,OR:2.61,95%CI:1.69-4.03),former smoking(OR:1.79,95%CI:1.15-2.79),and regular inhaled medication treatment(OR:3.28,95%CI:2.29-4.70)were

关键词： Chronic obstructive pulmonary disease Influenza Pneumococcal Vaccine AECOPD

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