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Anti-proliferative and pro-apoptotic effects of tectorigenin on hepatic stellate cells

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World Journal of Gastroenterology 2010年第31期16卷 3911-3918页

作者： jun-hua wu Yu-Rong Wang wu-Yang huang Ren-Xiang Tan Institute of Functional Biomolecules State Key Laboratory of Pharma ceutic al Biote chnologyNanjing UniversityNanjing 210093Jiangsu ProvinceChina

AIM: To investigate the effect of tectorigenin on proliferation and apoptosis of hepatic stellate cells (HSC)-T6 cells. METHODS: HSC-T6 cells were incubated with tectorigenin at different concentrations, and their proliferation was assessed by bromodeoxyuridine incorporation assay. Apoptosis was detected by flow cytometry assay with Hoechst 33342 staining. Also, generation of reactive oxygen species (ROS), intracellular [Ca2+]i, potential of mitochondrial membrane, activities of cytochrome c and caspase-9 and-3 were investigated to explore a conceivable apoptotic pathway. RESULTS: Tectorigenin suppressed the proliferation of HSC-T6 cells and induced apoptosis of HSC-T6 cells in a time-and dose-dependent manner. Tectorigenin at the concentration of 100 μg/mL greatly inhibited the viability of HSC-T6 cells and induced the condensation of chromatin and fragmentation of nuclei. When treated for 48 h, the percentage of cell growth and apoptosis reached 46.3% ± 2.37% (P = 0.004) and 50.67% ± 3.24% (P = 0.003), respectively. Furthermore, tectorigenin-induced apoptosis of HSC-T6 cells was associated with the generation of ROS, increased intracellular [Ca2+]i, loss of mitochondrial membrane potential, translocation of cytochrome c, and activation of caspase-9 and -3. CONCLUSION: Tectorigenin inhibits proliferation of HSC-T6 cells and induces apoptosis of HSC-T6 cells.

关键词： Tectorigenin Apoptosis Hepatic stellate cells Hepatic fibrosis Mitochondria Proliferation

Pro-apoptotic effects of tectorigenin on human hepatocellular carcinoma HepG2 cells

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World Journal of Gastroenterology 2012年第15期18卷 1753-1764页

作者： Chun-Ping Jiang Hui Ding Da-hua Shi Yu-Rong Wang Er-Guang Li jun-hua wu The First Clinical Medical College NanjingUniversity of Chinese Medicine Nanjing 210046 Jiangsu Province China Department of Hepatobiliary Surgery The Affiliated Drum Tower Hospital Medical School Nanjing University Nanjing 210008 Jiangsu Province China Jiangsu Key Laboratory of Molecular Medicine State Key Laboratory of Pharmaceutical Biotechnology Medical School Nanjing University Nanjing 210093 Jiangsu Province China

AIM:To investigate the effects of tectorigenin on human hepatocellular carcinoma(HCC)HepG2 ***:Tectorigenin,one of the main components of rhizome of Iris tectorum,was prepared by simple methods,such as extraction,filtration,concentration,precipitation and ***2 cells were incubated with tectorigenin at different concentrations,and their viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide *** was detected by morphological observation of nuclear change,agarose gel electrophoresis of DNA ladder,and flow cytometry with Hoechst 33342,Annexin V-EGFP and propidium iodide *** of reactive oxygen species was quantified using *** Ca2+was monitored by Fura *** membrane potential was monitored using Rhodamine *** of cytochrome c from mitochondria to cytosol was detected by Western *** of caspase-3,-8 and-9 were investigated by Caspase Activity Assay ***:The viability of HepG2 cells treated by tectorigenin decreased in a concentration-and timedependent *** concentration that reduced the number of viable HepG2 cells by 50%(IC50)after 12,24 and 48 h of incubation was 35.72 mg/L,21.19 mg/L and 11.06 mg/L,***,treatment with tectorigenin at 20 mg/L resulted in a very slight cytotoxicity to L02 cells after incubation for 12,24 or 48 *** at a concentration of 20 mg/L greatly inhibited the viability of HepG2 cells and induced the condensation of chromatin and fragmentation of *** induced apoptosis of HepG2 cells in a time-and dose-dependent *** with the viability rate,induction of apoptosis was the main mechanism of the anti-proliferation effect of tectorigenin in HepG2 ***,tectorigenininduced apoptosis of HepG2 cells was associated with the generation of reactive oxygen species,increased intracellular[Ca2+]i,loss of mitochondrial membrane potential,transloca

关键词： Tectorigenin Iris tectorum maxim Apop-tosis Hepatocellular carcinoma HepG2 Mitochondria Liver cancer

Synthesis of Cucurbit [ n ] uril-based Rotaxanes

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有机化学 2003年第Z1期23卷 261-262页

作者： LIU Si-Min wu Xiao-jun YAO jun-hua wu Cheng-Tai Department of Chemistry Wuhan University Wuhan 430072 Department of Chemistry Wuhan University Wuhan 430072 Instrumentation Analysis and Research Center Sun Yat-Sen University Guangzhou 510275 Department of Chemistry Wuhan University Wuhan 430072

　　Non-covalent attractive forces are commonly employed in biological systems to drive the assembly of highly orga nized supramolecular entities from relatively simple subunits.……

关键词： biological systems

Application of modified two-cuff technique and multiglycosides tripterygium wilfordii in hamster-to-rat liver xenotransplant model

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World Journal of Gastroenterology 2003年第7期9卷 1550-1553页

作者： hua Guo Yi-jun wu Shu-Sen Zheng Wei-Lin Wang jun Yu, Department of Hepatobiliary Surgery, First Affiliated Hospital, Medical College Zhejiang University, Hangzhou 310003, Zhejiang Province, China Department of Hepatobiliary Surgery First Affiliated Hospital Medical College of Zhejiang University 79 Qingchun Lu Hangzhou 310003 Zhejiang Province China. ggghua@***

AIM: To modify the hamster-to-rat liver xenotransplant technique to prevent postoperative complications, and to study the inhibiting effect of multiglycosides tripterygium wilfordii (TⅡ) on immune ***: Female golden hamsters and inbred male Wistar rats were used as donors and recipients, respectively. One hundred and twelve orthotopic liver xenotransplants were performed by Kamada′s cuff technique with *** 72 hour survival of the animal after operation was considered as a successful operation. When the established surgical model became stable, 30 of the latest 42 cases were divided into untreated control group (n=15) and TⅡgroup (n=15) at random. Survival of recipients was observed. Liver specimens were collected at 2 and 72 h from the operated animals and postmortem, respectively, for histological ***: The successfully operative rate of the 30operations was 80 %, and the survival of the control and TⅡ group was 7.1±0.35 was days and 7.2±0.52 days, respectively (t=0.087, P=0.931). The rate of conjunctival hyperemia in control group (100 %) differed significantly from that (31%)in TⅡ group (P=0.001). Rejection did not occur in both groups within 2 h postoperatively, but became obvious in control group at 72 h after surgery and mild in TⅡ group. Although rejections were obvious in both groups at death of recipients,it was less severe in TⅡ group than in control ***: This modified Kamada′s technique can be used to establish a stable hamster-to-rat liver xenotransplant model. Monotherapy with multiglycosides tripteryguiumwilfordii prolong survival of recipients.

关键词：多糖苷雷公藤异种肝移植免疫排斥反应动物实验

On the bounds of frequency-selective channel capacity with doubly correlated geometrical MIMO channel model

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The Journal of China Universities of Posts and Telecommunications 2010年第2期17卷 8-13页

作者： LI Yong-hua , Lü Tie-jun, wu Xiao-fei, ZHANG Lin Key Laboratory of Universal Wireless Communications, Ministry of Education, Beijing University of Posts and Telecommunications, Beijing 100876, China Key Laboratory of Universal Wireless Communications Ministry of Education Beijing University of Posts and Telecommunications Beijing 100876 China

The multi-input multi-output （MIMO） technology plays an important role in link transmissions. This article considers the general case for the ergodic capacity in doubly correlated frequency-selective MIMO channel. In the study, the geometrical MIMO channel model is presented. Based on the formula of MIMO ergodic capacity, the capacity limits are studied with arbitrary finite number of antennas in the frequency-selective MIMO channel. It first derives the exact expressions for the upper bound and lower bound in doubly correlated MIMO channel. The results for the single-ended correlation and independent identically distributed （i.i.d.） MIMO channel are also obtained as special cases. Then the simple expressions of the capacity bounds are attained at high SNR. Finally, results are provided by Monte Carlo simulations to verify the tightness of the derived bounds.

关键词： MIMO correlation capacity bounds

Scedosporium Apiospermum Infection after Near-drowning

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Chinese Medical Journal 2015年第15期128卷 2119-2123页

作者： Xin-hua He jun-Yuan wu Cai-jun wu Nicholas Van Halm-Lutterodt Jian Zhang Chun-Sheng Li Department of Emergency Medicine Beijing Chao-Yang Hospital Capital Medical University Beijing 100020 China

Scedosporium apiospermum （***,sexual form of Pseudallescheria boydii） is a highly invasive and opportunistic *** can invade various organs of the body,causing lethal *** is widely distributed in natural environments,including marshes,wetlands,sewage,putrilage,and salt *** incidence of *** infection can be especially high in humid temperate and subtropical regions.

关键词： Contaminated Water Near-drowning, Scedosporium apiospermum Voriconazole

Synethesis and Crystal Structure of Bi(ethylenediamine)copper Tetracyanonickelate(II)

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Chinese Journal of Structural Chemistry 2000年第3期19卷 187-190页

作者： LUO jun hua ②\ wu Mao Xiang\ WANG Yan Ming\ GAO Dong Shou Li Ding\ CHENG Chang Zhang (Fujian Insitute of Research on the Structure of Matter, the Chinese Academy of Sciences, Fuzhou, 350002) 中科院福建物质结构所福建福州 350 0 0 2

The crystal structure of the title compound C 8H 16 CuN 8Ni has been determined by single crystal X ray diffraction. The crystal is triclinic with space group P1, a=6.494(3), b=7.270(4), c=7.936(5) , α=106.67(3), β=91.33(4), γ=106.80(6)°, V=341.3(3)  3, Z=1, M r=346.54, F(000)=177, μ =2\^933mm -1 , D c =1.686 Mg/m 3. The final R factor is 0.0603 for 1214 unique observed reflections 〔 I≥2σ(I) 〕. The structure consists of 〔Cu(en) 2〕 2+ (en=ethylenediamine) cations and 〔Ni(CN) 4〕 2- anions linked together by two of the CN groups (the remaining two act as unidentate ligands) to form infinite chains, in the chain, the hydrogen bonds of N(4)…H-N(5) exist,with distance 3.148 , at the same time the hydrogen bonds of N(4)…H-N(7a) and N(5)-H…N(1a) (a: x-1,y-1,z-1 ) between the interchains also exist, with distances of 3.160 and 3.124 , so it forms a three\|dimensional structure of the title compound.

关键词： cyanide bridged complexes copper(II) complex crystal structure.

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我国名师研究的现状及前瞻

教育理论与实践 2017年第10期37卷 46-50页

作者：成俊华吴丽山西师范大学教师教育学院山西临汾041004 山西省教育科学研究院山西太原030009

以中国知网(CNKI)名师研究核心期刊及硕博论文数据为基础,对我国名师研究现状进行分析表明,当前我国关于名师的研究主要集中在名师成长、教学、培养、案例、评选、特征、内涵、作用及效应等九个方面。未来名师研究将向理论研究系统化、... 详细信息

以中国知网(CNKI)名师研究核心期刊及硕博论文数据为基础,对我国名师研究现状进行分析表明,当前我国关于名师的研究主要集中在名师成长、教学、培养、案例、评选、特征、内涵、作用及效应等九个方面。未来名师研究将向理论研究系统化、实践研究深入化、研究方法多样化、研究视角多元化方向发展。

关键词：名师研究成长教学培养评选特征

Synthesis of Cucurbit[ n ]uril-based Rotaxanes

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Synthesis of Cucurbit[ n ]uril-based Rotaxanes