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Testis-specific Fankl gene in knockdown mice produces oligospermia via apoptosis

Asian Journal of Andrology 2014年第1期16卷 124-130页

作者： Wan-Wei Dong hua-liang huang Wei Yang Jia Liu Yang Yu Sheng-Lai Zhou Wei Wang Xiang-Chuan Lv Zhao-Yang LP Mei-Ying Zhang Zhi-Hong Zheng Wei Yan Laboratory Animal Centre China Medical University Shenyang China Department of Clinical Laboratory The Third Affiliated Hospital of Inner Mongonia MedicalUniversity Baogang Hospital Baotou China Department of Pathology and Pathophysiology Research China Medical University Shenyang China and Department ofPhysiology and Cell Biology University of Nevada School of Medicine Reno Nevada USA.

Fankl is exclusively expressed in the testis from the meiosis phase to the haploid phase of spermatogenesis. In this study, we examined the function of Fankl by establishing a Fankl-knockdown transgenic mouse model. The apoptotic statuses of the testes of the transgenic mice were tested using the terminal deoxynucleotidyl transferase dUTP nick end labeling （TUNEL） method. The FANK1 consensus DNA-binding sequence was identified using cyclic amplification of sequence target （CAST） analysis. Differentially expressed genes were examined using microarray analysis. A reduction in sperm number and an increase in apoptotic spermatocytes were observed in Fankl-knockdown mice, and the apoptotic cells were found to be primarily spermatogonia and spermatocytes. The CAST results demonstrated that the consensus DNA-binding sequence was AAAAAG, in which the percentage occurrence of each base at each position ranged from 55 to 86%. This sequence was present in the promoter regions of 10 differentially expressed genes that were examined using microarray analysis. In total, 17 genes were differentially expressed with changes in their expression levels greater than twofold. The abnormal expression of Fankl target genes that were regulated directly or indirectly by Fankl reduced the number of sperm in the knockdown mice. Thus, FANK1 may Dlav a pivotal role in sDermato^enesis as a transcription *** is exclusively expressed in the testis from the meiosis phase to the haploid phase of spermatogenesis. In this study, we examined the function of Fankl by establishing a Fankl-knockdown transgenic mouse model. The apoptotic statuses of the testes of the transgenic mice were tested using the terminal deoxynucleotidyl transferase dUTP nick end labeling （TUNEL） method. The FANK1 consensus DNA-binding sequence was identified using cyclic amplification of sequence target （CAST） analysis. Differentially expressed genes were examined using microarray analysis, A reduction in sperm number an

关键词： Fankl knockdown mice spermatogenesis

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Synthesis,structure and electrochemistry of dithiocarbamato phenthiolate oxo-molybdenum(Ⅳ)complexes[MoO(SΦ)_2(S_2CNEt_2)]

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Chinese Journal of Chemistry 1993年第3期11卷 231-239页

作者： Zhuang,Bo-Tao PAN,Bing-hua huang,liang-Ren EAN,An-Jian Fujian Institute of Research on the Structure of Matter,Fuzhou Research Laboratory of Structural Chemistry,Chinese Academy of Sciences,Fuzhou,Fujian 350002 Fujian Institute of Research on the Structure of Matter Fuzhou Research Laboratory of Structural Chemistry Chinese Academy of Sciences Fuzhon Fujian 350002

The reaction of Mo(0) complex [Mo(CO)_4(S_2CNEt_2)]^- with phenthiolate [Et_4N]SΦ in acetonitrilo in the presence of small amount of air affords a new oxo-molybdenum com- plex [MoO(SΦ)_2(S_2CNEt_2)]^-,which crystallizes in two forms of crystals,[Et_4N][MoO(SΦ)_2 (S_2CNEt_2)](1a) and [Et_4N][MoO(SΦ)_2(S_2CNEt_2)]·(CH_3)_2CHOH (1b).The structures of 1a and 1b were determined from three-dimensional X-ray data.1a crystallizes in the mo- noclinie,space group C_c with a=12.321(4),b=15.245(4),c=16.087(9);β=98.44(4)°.V= 2989~3,Z=4,D_c=1.35 g/cm^3 and R=0.031 for 2434 reflections [I>3(I)].1b crystallizes in the monoclinic,space group P2_1/n with a=9.861(1),b=20.357(3),c=17.122(5)A;β=92.27 (2)°,V=3434.3~3,Z=4;D_c=1.29 g/cm^3 and R=0.051 for 2852 independeut reflections [I> 3σ(I)],The structures of 1a and 1b reveal that the anion [MoO(SΦ)_2(S_2CNEt_2)]^- contains a single oxo ligand coordinating to a molybdenum(Ⅳ) and the geometry around Me(Ⅳ) atom is a distorted square ***,the solvate molecule isopropanol of 1b is linked to oxo group by a hydrogen-bond of 1.928A,leading to the increase of Mo=O bond distance (1.718).Mo—S distances are 2.44 and 2,*** electrochemical behavior of 1 was discussed also.

关键词： Mo Synthesis,structure and electrochemistry of dithiocarbamato phenthiolate oxo-molybdenum complexes[MoO S2CNEt2

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Experimental Study on Diesel Spray and Flame Asymmetry Characteristics of Dual-Orifice Nozzles for a Medium-Speed Engine

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Journal of Thermal Science 2025年第1期34卷 303-322页

作者： JU Dehao LIU Rui ZHENG liang DENG Jiahui ZHANG Wenzheng HUO Jinlu huang Li Shanghai Marine Diesel Engine Research Institute National Key Laboratory of Marine Engine Science and Technology Key Laboratory for Power Machinery and Engineering Ministry of EducationShanghai Jiao Tong University

The asymmetry of the multi-orifice spray will cause uneven heat load of the marine diesel engine,thereby affecting its working performance and service ***,an in-depth understanding of the spray and flame characteristics of multi-orifice nozzles will guide the optimization of the nozzle structure,needle design and diesel atomization and combustion *** this reason,four groups of dual-orifice nozzles with different hole diameters(0.1-0.55 mm) and mass flow rates covering the typical marine medium-speed diesel injections are designed and customized,and the constant volume chamber(CVC) with high temperature and pressure is used to simulate the actual in-cylinder working conditions of the diesel engine for the spray visualization *** study the asymmetry of the fuel sprays discharged from a diesel injector,the multi-orifice nozzle is simplified as a dual-orifice nozzle in this *** with X-ray Computed Tomography(CT) imaging technology,the influences of the nozzle internal structure on the spray and flame asymmetry are studied in the constructed supercritical *** is found that the asymmetry of the inlet angle and the equivalent length-diameter ratio is positively correlated with the inconsistency of the dual *** an increase in the injection pressure and nozzle diameter,the asymmetry of the dual spray becomes more pronounced,resulting in greater disparities in the ignition delay times and ignition positions of the two ***,the increase in nozzle diameter also leads to combustion instability,resulting in a flame with a serrated *** the increase of ambient temperature,the proportion of liquid phase in the jet decreases and the relative density of spray front decreases.

关键词： atomization dual-orifice nozzle nozzle structure spray flame asymmetry

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Effect of flow velocity on corrosion behavior of AZ91D magnesium alloy at elbow of loop system

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Transactions of Nonferrous Metals Society of China 2016年第11期26卷 2858-2867页

作者：田径黄华良潘志权周红武汉工程大学化学与环境工程学院武汉430073 华中科技大学化学与化工学院武汉430074

A loop system was used to investigate the effect of flow velocity on corrosion behavior of AZ91 D magnesium alloy at an elbow of loop system based on array electrode technology by polarization, computational fluid dynamics(CFD) simulation and surface analysis. The experimental results showed that the corrosion rate increased with increasing flow velocity, and a critical flow velocity could exist in the corrosion of AZ91 D magnesium alloy. When flow velocity exceeded the critical flow velocity, fluid hydrodynamics was dominant in the corrosion of AZ91 D magnesium alloy. On the contrary, the electrochemical factors were dominant.

关键词： magnesium alloy flow velocity elbow computational fluid dynamics(CFD) polarization

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Amperometric Glucose Sensor Based on Batch CuO-Modified Screen-Printed Silver-Carbon Electrodes

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 35-41页

作者： XIA Haiyan huang Yin YANG hua WANG Xintian SHI Jianping LIU Lixia FU Yacheng QUAN Changyun LI Suyuan Cofoe Medical Technology Company Limited Changsha 410000China Institute of New Energy Hexi UniversityZhangye 734000China Chemistry and Chemical Engineering Central South UniversityChangsha 410000China

CuO nanoparticles were successfully synthesized via a two-jet electrospun method,and then screen-printed on silver-carbon electrodes,forming CuO-modified Ag-C(CuO/Ag-C)disposable strip *** natural environment condition for glucose detection,the obtained CuO/Ag-C electrodes show a high sensitivity of 540 nA·mM^(-1)·cm^(-2),and a low limit of detection(0.68 mM)in a wide linear response range of 0.68 mM and 3 mM(signal/noise=3),*** addition,the CuO/Ag-C electrodes also exhibit excellent anti-interference,air stability and *** a result,the fabrication of CuO nanoparticles via an electrospun process and the technique of screen-printed electrodes are of great significance for glucose detection.

关键词： electrochemical sensor CuO nanoparticles glucose detection screen-printing technique

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Buried interface bridging for inverted cesium-formamidinium triiodide perovskite solar cells with long operational stability

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Science China Chemistry 2025年第1期68卷 163-173页

作者： Chenhui Zhang Chunjun liang Fulin Sun Ting Zhu Xinghai huang Yuzhu Guo Xinyu Guo Kunyang Ge Dan Li Fangtian You Zhiqun He Key Laboratory of Luminescence and Optical Information Ministry of Education Institute of Optoelectronic TechnologyBeijing Jiaotong University Department of Physics School of Physical Science and Engineering Beijing Jiaotong University

In the field of perovskite solar cells(PSCs), the research on defects in the buried interface has been relatively limited due to its non-exposure; however, this interface significantly impacts the performance enhancement of inverted PSCs. This study employs phenylethylammonium chloride(PEACl) molecules as a buffer layer to modify the buried interface of p-i-n structured PSCs,aiming to enhance the uniformity of self-assembled monolayers(SAMs) and facilitate the uniform nucleation and growth of perovskite films on the substrate. Furthermore, the introduction of the PEACl buffer layer effectively passivates defects at the bottom of the perovskite layer and notably enhances the crystal quality of the perovskite film by mitigating residual stress,thereby reducing nonradiative recombination loss. Following these optimizations, the MA-free PSCs treated with PEACl achieve a power conversion efficiency(PCE) of 24.11%, with significant improvements in storage, thermal stability, and operational stability. Particularly noteworthy is the device's performance in an unencapsulated state, whereas after 1,500 hours of continuous light operation stability testing, it retains 97% of its original efficiency. This study not only enriches the systematic understanding of the characteristics of the buried interface in PSCs but also contributes significantly to advancing the commercial production of perovskite photovoltaic technology.

关键词： defect-induced recombination buried interfaces inverted perovskite solar cells crystallization lattice mismatch

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A promising approach for quantifying focal stroke modeling and assessing stroke progression:optical resolution photoacoustic microscopy photothrombosis

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Neural Regeneration Research 2025年第7期20卷 2029-2037页

作者： Xiao liang Xingping Quan Xiaorui Geng Yujing huang Yonghua Zhao Lei Xi Zhen Yuan Ping Wang Bin Liu Faculty of Health Sciences University of MacaoMacao Special Administrative RegionChina Center for Cognitive and Brain Sciences University of MacaoMacao Special Administrative RegionChina Department of Biomedical Engineering Southern University of Science and TechnologyShenzhenGuangdong ProvinceChina State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoTaipaMacao Special Administrative RegionChina Department of Emergency Zhujiang HospitalSouthern Medical UniversityGuangzhouGuangdong ProvinceChina

To investigate the mechanisms underlying the onset and progression of ischemic stroke,some methods have been proposed that can simultaneously monitor and create embolisms in the animal cerebral ***,these methods often require complex systems and the effect of age on cerebral embolism has not been adequately studied,although ischemic stroke is strongly *** this study,we propose an optical-resolution photoacoustic microscopy-based visualized photothrombosis methodology to create and monitor ischemic stroke in mice simultaneously using a 532 nm pulsed *** observed the molding process in mice of different ages and presented age-dependent vascular embolism ***,we integrated optical coherence tomography angiography to investigate age-associated trends in cerebrovascular variability following a *** imaging data and quantitative analyses underscore the differential cerebrovascular responses to stroke in mice of different ages,thereby highlighting the technique's potential for evaluating cerebrovascular health and unraveling age-related mechanisms involved in ischemic strokes.

关键词： age-dependent cerebral cortex ischemic stroke mouse model optical coherence tomography angiography photoacoustic microscopy photothrombosis vascular imaging

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Effects of direct current electric field on corrosion behaviour of copper, Cl- ion migration behaviour and dendrites growth under thin electrolyte layer

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Transactions of Nonferrous Metals Society of China 2014年第1期24卷 285-291页

作者：黄华良潘志权郭兴蓬邱于兵武汉工程大学化工与制药学院武汉430073 华中科技大学化学与化工学院武汉430074

Effect of direct current electric field （DCEF） on corrosion behaviour of copper printed circuit board （PCB-Cu）, Cl-ion migration behaviour, dendrites growth under thin electrolyte layer was investigated using potentiodynamic polarization and scanning electron microscopy （SEM） with energy dispersive spectrometer （EDS）. Results indicate that DCEF decreases the corrosion of PCB-Cu;Cl-ions directionally migrate from the negative pole to the positive pole, and enrich on the surface of the positive pole, which causes serious localized corrosion; dendrites grow on the surface of the negative pole, and the rate and scale of dendrite growth become faster and greater with the increase of external voltage and exposure time, respectively.

关键词： copper dendrites migration direct current electric field thin electrolyte layer copper printed circuit board

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Gas-Solid Flow,Combustion Characteristics,and Gas Emissions in a 75 t/h CFB Boiler Based on the CPFD Method

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Journal of Thermal Science 2025年第1期34卷 323-336页

作者： huang Xiankun BAI Zhang ZHU Xiaoli WANG Shuoshuo MU Lin GONG liang College of New Energy China University of Petroleum (East China) Key Laboratory of Ocean Energy Utilization and Energy Conservation of Ministry of Education School of Energy and Power EngineeringDalian University of Technology

A 3D simulation using Computational Particle Fluid Dynamics（CPFD） methods was used to calculate coal combustion in a 75 t/h industrial-scale circulating fluidized bed（CFB） *** characteristics,gas-solid flow characteristics,and gaseous pollutant emissions of CFB boilers from combustion ignition to stable operation were systematically evaluated in this *** show that the temperature distribution is relatively uniform throughout the *** the combustion process unfolds within the boiler,the gas composition curve strikingly portrays the inverse correlation between CO2and *** the combustion reaction progresses,it becomes evident that the concentration of CO2progressively increases,while the concentration of O2concurrently *** inverse relationship underscores the fundamental combustion reaction,where carbon-based fuels react with oxygen to produce carbon dioxide and release ***,a comprehensive analysis has revealed that,from ignition to stable combustion,both nitric oxide（NO） and sulfur dioxide（SO2） emissions exhibit a declining *** reduction in pollutant generation is attributed to the improvement in combustion *** complete combustion leads to lower levels of unburned hydrocarbons,which are prone to NO ***,the sulfur content in the fuel is more efficiently oxidized to sulfur trioxide（SO3） or bound in sulfates,reducing *** steady state in the simulation,the SO2mass flow rate varies significantly with the furnace height,gradually increasing from 0.07 kg·s-1at 4 m at the bottom of the furnace to a peak of 0.078 kg·s-1at 8 m in the center,and then decreasing to 0.06 kg·s-1at 20m at the top of the furnace.

关键词： Sulfur dioxide

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Deciphering the role of ultra-low-loaded rhodium in NiFe-MIL-53 for superior oxygen evolution reaction

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Journal of Energy Chemistry 2025年第1期100卷 77-86页

作者： Jinzhi Jia Jinhua Zhang Kailu Guo Lanyue Zhang Gening Du Hao You Junfeng huang Mudong Tu hua Li Yong Peng Wei Dou Cailing Xu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical Engineering School of Materials and Energy Lanzhou University College of Chemistry and Chemical Engineering Luoyang Normal University

Designing highly active and stable electrocatalysts of oxygen evolution reaction （OER） is one of the crucial *** this study,a novel OER electrocatalyst,NiFe-MIL-53 modified with ultra-low rhodium（Rh@NiFe-MIL-53）,is successfully prepared via the hydrothermal ***-situ Raman spectroscopy and electrochemical impedance spectroscopy reveal that the doped Rh accelerates the phase transformation of NiFe-MIL-53 and the in-situ formed Rh@NiFeOOH is the actual active *** importantly,the enhanced reversibility of electrochemical reconstruction between NiFeOOH and NiFe（OH）2after doping Rh is beneficial for improving the electrochemical stability of the catalyst.X-ray photoelectron spectroscopy spectra show the strong electronic interaction between single-atom Rh and Ni/Fe in Rh@***,theoretical calculations confirm that the integration of single-atom Rh into the NiFeOOH successfully reduces the band gap,regulates the d-band center （εd）,accelerates the charge transfer,and optimizes the adsorption behavior of oxygen-containing intermediates,thereby lowering the energy barrier of rate-determining ***,the optimized Rh@NiFe-MIL-53 exhibits excellent OER activity （240 mV） with a small Tafel slope of 48.2 mV dec-1and long-term durability (over1270 h at 10 m A cm-2and 110 h at 200 mA cm-2).This work presents a new perspective on designing highly efficient OER electrocatalysts.

关键词： X ray photoelectron spectroscopy

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