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Epidermal growth factor receptor-targeted ultra-small superparamagnetic iron oxide particles for magnetic resonance molecular imaging of lung cancer cells in vitro

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Chinese Medical Journal 2012年第13期125卷 2322-2328页

作者： CHEN Chun-li hu guang-yuan MEI Qi QIU Hong LONG Guo-xian hu Guo-qing Cancer Center Tongji HospitalTongji Medical CollegeHuazhongUniversity of Science and TechnologyWuhanHubei 430030China

Background Magnetic resonance （MR） molecular imaging can detect abnormalities associated with disease at the level of cell and molecule. The epidermal growth factor receptor （EGFR） plays an important role in the development of lung cancer. This study aimed to explore new MR molecular imaging targeting of the EGFR on lung cancer cells. Methods We attached ultra-small superparamagnetic iron oxide （USPIO） particles to cetuximab （C225） anti-human IgG using the carbodiimide method. We made the molecular MR contrast agents C225-USPIO and IgG-USPIO, the latter as a control reagent, and determined concentrations according to the Fe content. Lung cancer A549 cells were cultured and immunocytochemistry （SP） was used to detect the expression of EGFR on cells. We detected the binding rate of C225-USPIO to A549 cells with immunofluorescence staining and flow cytometry. We cultured A549 cells with C225-USPIO at a Fe concentration of 50 pg/ml and assayed the binding of C225-USPIO after 1 hour with Prussian blue staining and transmission electron microscopy （TEM）. We determined the effects on imaging of the contrast agent targeted to cells using a 4.7T MRI. We did scanning on the cells labeled with C225-USPIO, IgG-USPIO, and distilled water, respectively. The scanning sequences included axial T1WI, T2WI. Results Immunocytochemical detection of lung cancer A549 cells found them positive for EGFR expression. Immunofluorescence staining and flow cytometry after cultivation with different concentrations of C225-USPIO showed the binding rate higher than the control. Prussian blue staining and transmission electron microscopy revealed that in the C225-USPIO contrast agent group of cells the particle content of Fe in cytoplasmic vesicles or on surface was more than that in the control group. The 4.7T MR imaging （MRI） scan revealed the T2WI signal in the C225-USPIO group of cells decreased significantly more than in unlabeled cells, but there was no significant difference between the time

关键词： ultra-small superparamagnetic iron oxide epidermal growth factor receptor,"magnetic resonance imaging," target

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强、弱随附性概念探析

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自然辩证法研究 2016年第12期32卷 14-18页

作者：胡光远中原工学院河南郑州450007

随附性是个意蕴丰富的哲学概念,金在权依据不同的模态强度区分了强随附性和弱随附性。但是,金在权对随附性的双条件句描述犯有错误,而且他的必然性没有一致地给出弱随附性和强随附性的跨世界解释。区分强、弱随附性的关键是认识随附性... 详细信息

随附性是个意蕴丰富的哲学概念,金在权依据不同的模态强度区分了强随附性和弱随附性。但是,金在权对随附性的双条件句描述犯有错误,而且他的必然性没有一致地给出弱随附性和强随附性的跨世界解释。区分强、弱随附性的关键是认识随附性的依赖与决定关系的不对称性,从这一角度可以对强、弱随附性中出现的"必然"给出合理说明,也能够以此修正强、弱随附性的划分。

关键词：随附性强随附性和弱随附性必然依赖必然决定模态算子

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Specific targeting of angiogenesis in lung cancer with RGD-conjugated ultrasmall superparamagnetic iron oxide particles using a 4.7T magnetic resonance scanner

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Chinese Medical Journal 2013年第12期126卷 2242-2247页

作者： LIU Can LIU Dong-bo LONG Guo-xian WANG Jun-feng MEI Qi hu guang-yuan QIU Hong hu Guo-qing Cancer Center Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430030 China Department of Oncology Puai Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430035 China

Background Angiogenesis is an essential step for tumor development and *** cell adhesion molecule αvβ3 integrin plays an important role in angiogenesis and is a specific marker of tumor angiogenesis.A novel αvβ3 integrintargeted magnetic resonance （MR） imaging contrast agent utilizing Arg-Gly-Asp （RGD） and ultrasmall superparamagnetic iron oxide particles （USPIO）（referred to as RGD-USPIO） was designed and its uptake by endothelial cells was assessed both in vitro and in vivo to evaluate the angiogenic profile of lung *** USPIO were coated with-NH3＋ and conjugated with RGD *** blue staining was performed to evaluate the specific uptake of RGD-USPIO by human umbilical vein endothelial cells （huVECs）.Targeted uptake and subcellular localization of RGD-USPIO in huVECs were confirmed by transmission electron microscopy （TEM）.The ability of RGD-USPIO to noninvasively assess αvβ3 integrin positive vessels in lung adenocarcinoma A549 tumor xenografts was evaluated with a 4.7T MR *** was used to detect αvβ3 integrin expression and vessel distribution in A549 tumor *** huVECs internalized RGD-USPIO significantly more than plain *** uptake of RGD-USPIO by huVECs could be competitively inhibited by addition of free RGD.A significant decrease in T2 signal intensity （SI） was observed at the periphery of A549 tumor xenografts at 30 minutes （P 〈0.05） and 2 hours （P 〈0.01） after RGD-USPIO was injected via the tail *** blood vessels were mainly distributed in the periphery of tumor xenografts with positive αvβ3 integrin *** RGD-USPIO could specifically label αvβ3 integrin and be taken up by *** molecular MR imaging contrast agent can specifically evaluate the angiogenic profile of lung cancer using a 4.7T MR scanner.

关键词： ultrasmall superparamagnetic iron oxide particles Arg-Gly-Asp avfβ magnetic resonance imaging

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Unraveling brain aging through the lens of oral microbiota

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Neural Regeneration Research 2025年第7期20卷 1930-1943页

作者： Qinchao hu Si Wang Weiqi Zhang Jing Qu guang-hui Liu State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Hospital of Stomatology Guanghua School of StomatologyGuangdong Provincial Key Laboratory of StomatologySun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Advanced Innovation Center for human Brain Protection and National Clinical Research Center for Geriatric Disorders Xuanwu HospitalCapital Medical UniversityBeijingChina Aging Translational Medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijingChina CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijingChina University of Chinese Academy of Sciences BeijingChina Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijingChina Aging Biomarker Consortium BeijingChina State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Beijing Institute for Stem Cell and Regenerative Medicine BeijingChina

The oral cavity is a complex physiological community encompassing a wide range of *** of oral microbiota can lead to various oral infectious diseases,such as periodontitis and tooth decay,and even affect systemic health,including brain aging and neurodegenerative *** studies have highlighted how oral microbes might be involved in brain aging and neurodegeneration,indicating potential avenues for intervention *** this review,we summarize clinical evidence demonstrating a link between oral microbes/oral infectious diseases and brain aging/neurodegenerative diseases,and dissect potential mechanisms by which oral microbes contribute to brain aging and *** also highlight advances in therapeutic development grounded in the realm of oral microbes,with the goal of advancing brain health and promoting healthy aging.

关键词： Alzheimer's disease brain aging multiple sclerosis neurodegeneration neurodegenerative diseases oral microbiota Parkinson's disease periodontitis bacteria Porphyromonas gingivalis

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Dynamic Regulation of Hydrogen Bonding Networks and Solvation Structures for Synergistic Solar‑Thermal Desalination of Seawater and Catalytic Degradation of Organic Pollutants

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Nano-Micro Letters 2025年第2期17卷 548-565页

作者： Ming‑yuan Yu Jing Wu guang Yin Fan‑Zhen Jiao Zhong‑Zhen Yu Jin Qu Beijing Key Laboratory of Advanced Functional Polymer Composites Beijing University of Chemical TechnologyBeijing 100029People’s Republic of China State Key Laboratory of Organic‑Inorganic Composites College of Materials Science and EngineeringBeijing University of Chemical TechnologyBeijing 100029People’s Republic of China

Although solar steam generation strategy is efficient in desalinating seawater,it is still challenging to achieve continuous solar-thermal desalination of seawater and catalytic degradation of organic ***,dynamic regulations of hydrogen bonding networks and solvation structures are realized by designing an asymmetric bilayer membrane consisting of a bacterial cellulose/carbon nanotube/Co_(2)(OH)_(2)CO_(3)nanorod top layer and a bacterial cellulose/Co_(2)(OH)_(2)CO_(3)nanorod(BCH)bottom ***,the hydrogen bonding networks inside the membrane can be tuned by the rich surface–OH groups of the bacterial cellulose and Co_(2)(OH)_(2)CO_(3)as well as the ions and radicals in situ generated during the catalysis ***,both SO_(4)^(2−)and HSO_(5)−can regulate the solvation structure of Na^(+)and be adsorbed more preferentially on the evaporation surface than Cl^(−),thus hindering the de-solvation of the solvated Na^(+)and subsequent nucleation/growth of ***,the heat generated by the solar-thermal energy conversion can accelerate the reaction kinetics and enhance the catalytic degradation *** work provides a flow-bed water purification system with an asymmetric solar-thermal and catalytic membrane for synergistic solar thermal desalination of seawater/brine and catalytic degradation of organic pollutants.

关键词： Solar steam generation Seawater desalination Catalytic degradation Bacterial cellulose Cobalt hydroxycarbonate nanorods

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数据挖掘方法在用户流失预测分析中的应用

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计算机工程与应用 2007年第9期43卷 154-156页

作者：刘光远苑森淼董立岩吉林大学通信工程学院长春130022 吉林大学计算机科学与技术学院长春130012

移动通信在高速发展的同时,出现了大量用户离网的现象,基于客户信息、消费行为等历史数据,进行客户离网预测分析成为各个运营商普遍关注的问题。文章基于客户的历史数据和短期偶发数据,提出了链型数据挖掘方法,并结合决策树,形成了一个... 详细信息

移动通信在高速发展的同时,出现了大量用户离网的现象,基于客户信息、消费行为等历史数据,进行客户离网预测分析成为各个运营商普遍关注的问题。文章基于客户的历史数据和短期偶发数据,提出了链型数据挖掘方法,并结合决策树,形成了一个综合的链型树分类器(Chain Tree Classifier,CTC)和用户行为预测模型,实验结果显示,该分类器对移动通信运营商感兴趣的单个事件发生具有良好的预测能力,可被应用到客户离网预测中,从而帮助运营商提前发现具有离网倾向的用户,进而获得更高的利润。

关键词：数据挖掘链型树分类器用户流失预测

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网络社会思潮的内涵分析

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中原工学院学报 2016年第2期27卷 67-70,92页

作者：胡光远中原工学院郑州450007

社会思潮是社会意识运动的结果,是对社会存在的反映,既依附于它的原像社会存在,又离不开人的思维。在网络社会中,一切传统的生产生活围绕着资本、管理与信息的全球网络而组织起来。网络把现实世界的物质实在与精神实在转换成符码,网络... 详细信息

社会思潮是社会意识运动的结果,是对社会存在的反映,既依附于它的原像社会存在,又离不开人的思维。在网络社会中,一切传统的生产生活围绕着资本、管理与信息的全球网络而组织起来。网络把现实世界的物质实在与精神实在转换成符码,网络社会思潮就是以符码形式存在的、具有自身逻辑且通过网络传播的社会思潮。

关键词：网络社会思潮动态内涵

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Enhancing m^(6)A modification in the motor cortex facilitates corticospinal tract remodeling after spinal cord injury

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Neural Regeneration Research 2025年第6期20卷 1749-1763页

作者： Tian Qin Yuxin Jin Yiming Qin Feifei yuan Hongbin Lu Jianzhong hu Yong Cao Chengjun Li Department of Spine Surgery and Orthopedics Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina Key Laboratory of Organ Injury Aging and Regenerative Medicine of Hunan ProvinceChangshaHunan ProvinceChina National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina Department of Sports Medicine Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina

Spinal cord injury typically causes corticospinal tract disruption. Although the disrupted corticospinal tract can self-regenerate to a certain degree, the underlying mechanism of this process is still unclear. N6-methyladenosine(m^(6)A) modifications are the most common form of epigenetic regulation at the RNA level and play an essential role in biological processes. However, whether m^(6)A modifications participate in corticospinal tract regeneration after spinal cord injury remains unknown. We found that expression of methyltransferase 14 protein(METTL14) in the locomotor cortex was high after spinal cord injury and accompanied by elevated m^(6)A levels. Knockdown of Mettl14 in the locomotor cortex was not favorable for corticospinal tract regeneration and neurological recovery after spinal cord injury. Through bioinformatics analysis and methylated RNA immunoprecipitation-quantitative polymerase chain reaction, we found that METTL14 regulated Trib2 expression in an m^(6)A-regulated manner, thereby activating the mitogen-activated protein kinase pathway and promoting corticospinal tract regeneration. Finally, we administered syringin, a stabilizer of METTL14, using molecular docking. Results confirmed that syringin can promote corticospinal tract regeneration and facilitate neurological recovery by stabilizing METTL14. Findings from this study reveal that m^(6)A modification is involved in the regulation of corticospinal tract regeneration after spinal cord injury.

关键词： corticospinal tract remodeling epigenetic regulations locomotor cortex m^(6)A modification methyltransferase 14 protein(METTL14) mitogen-activated protein kinase neural regeneration spinal cord injury syringin TRIB2

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国产帕洛诺司琼预防化疗所致不良反应的临床观察

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中华医院感染学杂志 2010年第13期20卷 1929-1930页

作者：龙国贤胡广原华中科技大学同济医学院附属同济医院肿瘤中心湖北武汉430030

目的评价国产帕洛诺司琼(吉欧停)预防化疗所致恶心呕吐等不良反应的疗效和安全性。方法随机将接受高致吐风险化疗的患者分为吉欧停组(试验组)及托烷司琼组(对照组),观察两组的急性呕吐、延迟性呕吐及恶心的发生率和毒性反应。结果共入组... 详细信息

目的评价国产帕洛诺司琼(吉欧停)预防化疗所致恶心呕吐等不良反应的疗效和安全性。方法随机将接受高致吐风险化疗的患者分为吉欧停组(试验组)及托烷司琼组(对照组),观察两组的急性呕吐、延迟性呕吐及恶心的发生率和毒性反应。结果共入组107例患者,试验组54例,对照组53例;试验组的急性呕吐完全缓解率明显高于对照组(85.2%比67.9%,P<0.05),试验组的延迟性呕吐完全控制率明显高于对照组(68.5%比47.2%,P<0.05),两组的毒性反应发生率相似,未观察到3～4级毒性反应。结论国产帕洛诺司琼预防高致吐风险化疗所致呕吐优于托烷司琼,毒性轻微,值得临床常规应用。

关键词：帕洛诺司琼托烷司琼化疗不良反应

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莫言小说中的寺院意象

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渤海大学学报（哲学社会科学版） 2005年第5期27卷 35-37页

作者：刘广远渤海大学中文系辽宁锦州121013

莫言小说中经常出现寺院意象。寺院里的“神”和“圣徒”等宗教象征符号被创作主体消解和颠覆,摧毁了神圣的宗教情感和宗教尊严,使“神本位”回到“人本位”,甚至“人体本位”。

关键词：寺院神宗教

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