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天然橡胶手套中水抽提蛋白含量测定影响因素的研究

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质量安全与检验检测 2024年第4期34卷 63-68页

作者：向婧刘婷婷吴迪张莺莺刘路张芬胡红刚江西省医疗器械检测中心江西南昌330029

当前对天然胶乳手套(以下简称手套)中蛋白含量测定方法主要引用国家标准GB/T 21870—2008,该方法未明确提取过程(本研究主要针对方法A-剪碎手套抽题方法)条件,测定影响因素众多。本文通过单因素实验研究抽提过程中各个因素,如手套剪碎... 详细信息

当前对天然胶乳手套(以下简称手套)中蛋白含量测定方法主要引用国家标准GB/T 21870—2008,该方法未明确提取过程(本研究主要针对方法A-剪碎手套抽题方法)条件,测定影响因素众多。本文通过单因素实验研究抽提过程中各个因素,如手套剪碎程度、抽提液的用量、提取温度、提取时间以及震荡频率等因素的影响,得到最优提取条件。用缓冲液抽提水溶性蛋白质,经通过沉淀、浓缩,再溶解沉淀的蛋白质,以标准蛋白质做参照,用改进Lowry方法进行比色,定量测定蛋白质含量。结果显示,在手套剪碎大小为(3~10)mm×(3~10)mm,液料比为20∶1,提取次数为2次,温度为35℃,振荡速度为120 r/min时,标准曲线回归方程为y=0.01138x+0.08734,r2=0.99965,在5.40μg/mL~54.03μg/mL范围内,有良好的线性关系,平均重复性RSD为0.96%,平均加样回收率为101.81%。表明该试验方法结果更准确可靠,能够为手套蛋白提取工艺的明确提供实验依据。

关键词：天然橡胶手套水抽提蛋白单因素方法学验证

白介素13与白喉毒素融合蛋白靶向治疗活性的研究

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医学研究杂志 2008年第10期37卷 31-36,F0003页

作者：杜娟郑妍鹏孙维敏胡红刚侯玲玲洪涛北京交通大学理学院生物科学与技术研究所 100044

目的研究白介素13与白喉毒素融合蛋白DT389-hIL13-13E13K对神经胶质瘤的靶向杀伤作用,为神经胶质瘤的无创性治疗和常规化疗的替代或补充治疗提供新的途径。方法通过诱导已构建的融合蛋白表达质粒,原核表达并获得IL-13与白喉毒素融合蛋白... 详细信息

目的研究白介素13与白喉毒素融合蛋白DT389-hIL13-13E13K对神经胶质瘤的靶向杀伤作用,为神经胶质瘤的无创性治疗和常规化疗的替代或补充治疗提供新的途径。方法通过诱导已构建的融合蛋白表达质粒,原核表达并获得IL-13与白喉毒素融合蛋白DT389-hIL13-13E13K。分别通过两种不同方法提纯后,用体内外实验研究不同浓度融合蛋白对神经胶质瘤的靶向杀伤活性。结果所得融合蛋白浓度纯度均达到95%以上。在体外活性实验中,两种复性纯化方法获得的融合蛋白DT389-hIL13-13E13K对神经胶质瘤均有很好的杀伤效果,半数抑制浓度(IC50)分别达到6×10-10mol/L和1×10-8mol/L,杀伤效果达到了国际水平。体内活性测定结果显示,融合蛋白DT389-hIL13-13E13K对神经胶质瘤有一定的抑制作用,但效果并未达到使肿瘤完全消退的水平。结论得到了在体内外都对神经胶质瘤有抑制杀伤作用的特异的靶向治疗融合蛋白。

关键词：神经胶质瘤肿瘤治疗白介素13 白喉毒素融合蛋白

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青霉属生物中分离出的抗真菌四肽的固相合成

药学实践杂志 2014年第5期32卷 342-343,378页

作者：杨璐晶王露李文娟李仁武王晓燕胡宏岗第二军医大学研究生管理大队上海200433 第二军医大学药学院上海200433

目的以固相合成法合成抗真菌四肽D-Phe-Val-D-Val-Tyr-OH。方法采用固相合成法通过4步反应顺利获得目标四肽,所得化合物的结构通过MS、1H NMR等光谱确证。结果合成得到了抗真菌四肽D-Phe-Val-D-Val-Tyr-OH,总收率为47.0%。结论合成方法... 详细信息

目的以固相合成法合成抗真菌四肽D-Phe-Val-D-Val-Tyr-OH。方法采用固相合成法通过4步反应顺利获得目标四肽,所得化合物的结构通过MS、1H NMR等光谱确证。结果合成得到了抗真菌四肽D-Phe-Val-D-Val-Tyr-OH,总收率为47.0%。结论合成方法具有可行性,操作简便、总收率高,目标化合物可用于抗真菌药物研究。

关键词：抗真菌四肽固相合成

超高效液相色谱-串联质谱法快速定性和定量筛查贴敷类医疗器械产品非法添加的水杨酸甲酯

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医疗装备 2024年第5期37卷 39-43页

作者：刘路皮璟渔余永红刘小辉胡红刚梁国东江西省医疗器械检测中心江西南昌330000

目的通过建立一种利用超高效液相色谱-串联质谱法(UPLC-MS/MS),对贴敷类医疗器械非法添加的水杨酸甲酯进行定性、定量分析。方法样品经甲醇提取、过滤后上机,采用超高效液相色谱-串联质谱法,WATERS ACQUITY UPLC BEH C18色谱柱(2.1mm×1... 详细信息

目的通过建立一种利用超高效液相色谱-串联质谱法(UPLC-MS/MS),对贴敷类医疗器械非法添加的水杨酸甲酯进行定性、定量分析。方法样品经甲醇提取、过滤后上机,采用超高效液相色谱-串联质谱法,WATERS ACQUITY UPLC BEH C18色谱柱(2.1mm×100mm,1.7μm)分离,以0.05%氨水-甲醇为流动相,进行梯度洗脱;电喷雾电离(ESI),负离子扫描,多反应监测(MRM)模式采集数据。结果水杨酸甲酯1~100μg/ml范围内线性关系良好,相关系数为0.9997;3个添加水平平均加样回收率为90.3%~100.7%;相对标准偏差(RSD)为0.5%~0.9%;方法检出限为0.1μg/ml;定量限为0.3μg/ml;114份样品中,共检出阳性样品29份,检出率为25.4%,含量为每贴1~47mg,且阳性样品多集中在医用冷敷贴、远红外镇痛贴样品中。结论该方法准确度高、快捷灵敏,适用于贴敷类医疗器械产品非法添加的水杨酸甲酯进行定性与定量分析。

关键词：贴敷类医疗器械水杨酸甲酯超高效液相色谱-串联质谱法

Phase formation,texture evolutions,and mechanical behaviors of Al0.5CoCr0.8FeNi2.5V0.2 high-entropy alloys upon cold rolling

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Progress in Natural Science:Materials International 2022年第2期32卷 196-205页

作者： Tongtong Sun Weidong Song Feilong Shan Kaikai Song Kun Zhang Chuanxiao Peng honggang Sun Lina hu School of Mechanical Electrical & Information Engineering Shandong University State Key Laboratory of Explosion Science and Technology Beijing Institute of Technology CAS Key Laboratory of Microgravity (National Microgravity Laboratory) Institute of Mechanics Chinese Academy of Sciences Key Laboratory for Liquid-Solid Structural Evolution & Processing of Materials (Ministry of Education) Shandong University

The cold rolling(CR) reduction dependence of microstructure evolutions and mechanical properties for Al0.5CoCr0.8FeNi2.5V0.2high-entropy alloy(HEA) were *** HEA remains FCC structures consisting of nanoscale ordered L12phase,confirming the phase formation *** increasing CR reduction,the textures transform from random ones to FCC rolling ones accompanied by dense slip bands and deformation *** a 50% CR reduction,the deformation textures started to become evident and were governed by typical {111}F,{110}Goss and {112}Cu texture *** the CR reduction approached 90%,the deformation textures mainly contained the {110}A,{114}X,and {112}Cu texture *** a result,both Vickers hardness and ultimate strength increased,but the ductility decreased *** enhanced strength should be attributed to the anisotropy and work hardening behavior from *** plastic deformation for the samples under low CR reductions was dominated by deformation twins and slip ***,under high CR reductions,the textures severely impeded the further propagation of pre-existing slip bands,leading to the rapid decrease of ***,the CR reduction should be carefully designed before optimal heat treatments to enhance the strength and ductility synergy.

关键词： High-entropy alloys Cold rolling Texture Mechanical property Ductility

Transcriptomes of Litopenaeus vannamei reveal modulation of antioxidant system induced by dietary archaeal carotenoids

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Journal of Oceanology and Limnology 2023年第5期41卷 2010-2019页

作者： Wei XIE Guoru DU honggang DENG Yingchao MA Meirong GAO hu DUAN Sung YIK YEONG Liying SUI State Key Laboratory of Food Nutrition and Safety Tianjin University of Science&TechnologyTianjin 300457China Asian Regional Artemia Reference Center College of Marine and Environmental SciencesTianjin University of Science and TechnologyTianjin 300457China Institute of Marine Biotechnology(IMB) Universiti Malaysia Terengganu(UMT)Kuala NerusTerengganu 21030Malaysia

Oxidative stress induced by factors such as ammonia nitrogen has become a major issue in shrimp *** effects of carotenoids on the growth and antioxidant capability of Litopenaeus vannamei juveniles were investigated in this study using dietary archaeal carotenoids *** four weeks,shrimp were given diets containing 0 mg/kg(Ctrl)and 55.98 mg/kg(Car)archaeal *** archaeal carotenoids significantly enhanced the astaxanthin content in shrimp muscles and carapaces,as well as the superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)activity(P<0.05).The malonaldehyde(MDA)content in Car group significantly decreased(P<0.05).The transcriptome analysis was conducted to determine the molecular processes in response to archaeal carotenoids supplementation.A total of 1536 differentially expressed genes(DEGs)were detected,including 538 upregulated DEGs and 998 downregulated *** functional enrichment analysis between Ctrl and Car indicated that 26 GO terms including extracellular region,metabolic process,and proteolysis were *** KEGG pathway enrichment analysis revealed that the amino sugar and nucleotide sugar metabolism,cysteine and methionine metabolism,glycine serine and threonine metabolism,and amino acid biosynthesis were *** carotenoids influenced the expression of several important genes involved in reactive oxygen species(ROS)generation,Nrf2 signaling,and antioxidant *** DEGs were chosen to confirm the RNA-Seq data using *** genes and pathways discovered in this work assist to elucidate the molecular processes through which archaeal carotenoid enhances *** antioxidative system.

关键词： archaeal carotenoid Litopenaeus vannamei transcriptome antioxidative mechanism

Sensitivity correction of co-vibrating vector hydrophone in standing wave tube by numerical calculation

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Chinese Journal of Acoustics 2023年第4期42卷 464-478页

作者： ZHANG hu CHEN Hongjuan ZHANG honggang WANG Peng College of Ordnance Engineering Naval University of EngineeringWuhan 430033 College of Underwater Acoustic Engineering Harbin Engineering UniversityHarbin 150001

When the sensitivity of a large-size vector hydrophone is calibrated in a standing wave tube, the distribution of the calibration sound field will be uneven, resulting in a large error in the calibration results. In the case that the accurate analytical expression of the calibration sound field cannot be obtained, the sound field simulation analysis model is established based on the actual standing wave tube calibration device. The sound pressure and acceleration values of vector hydrophone and reference scalar hydrophone are obtained by numerical calculation,and the sensitivity correction factor is derived by combining the sensitivity calibration formula and the reference value. After using the correction factor, the maximum absolute error between the test results and the reference value of vector hydrophones with different sizes is reduced to less than 2.0 d B. The results show that the numerical calculation method can effectively reduce the sensitivity calibration error of large-size vector hydrophones caused by the non-uniform sound field and expand the test object of the vector hydrophone standing wave tube calibration device.

关键词： correction numerical wave

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A simple fiber length measurement method with improved accuracy using microwave photonic technology

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Optoelectronics Letters 2021年第10期17卷 626-629页

作者： ZHANG Ailing YAN Jinmeng PAN honggang hu Guang Engineering Research Center of Communication Devices and Technology Ministry of EducationTianjin Key Laboratory of Film Electronic and Communication DevicesSchool of Electrical and Electronic EngineeringTianjin University of TechnologyTianjin 300384China

A simple method to measure the length of optical fiber is proposed using microwave photonic *** length of fiber is measured according to the free spectral range(FSR)of the microwave photonic *** measurement accuracy is only related to the accuracy of FSR *** accuracy is further improved by introducing a reference fiber,and the method was verified in the experiment with 2-10 m single-mode fiber.

关键词： fiber measurement. microwave

Diflurobenzothiadiazole core-based noncovalently fused small molecule acceptor exhibiting over 12% efficiency and high fill factor

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Journal of Energy Chemistry 2020年第12期29卷 7-13页

作者： Qin Chang honggang Chen Jun Yuan Yunbin hu Jiefeng Hai Wei Liu Fangfang Cai Juan Hong Xuxian Xiao Yingping Zou College of Chemistry and Chemical Engineering Central South UniversityChangsha 410083HunanChina Guangxi Key Laboratory of Electrochemical and Magneto-Chemical Functional Materials College of Chemistry and BioengineeringGuilin University of TechnologyGuilin 541004GuangxiChina School of Materials Science and Engineering Zhengzhou UniversityZhengzhou 450001HenanChina

The versatility and flexibility of organic photoelectric materials endow organic photovoltaic cells fine function modulation and huge commercial potential. In this work, a new noncovalent fused-ring small molecule acceptor(SMA) BID-4 F has been synthesized for high-efficient organic solar cells(OSCs). BID-4 F consists of a diflurobenzothiadiazole(DFBT) core, ladder-like indacenodithiophene(IDT) spacers, and dicyanoindanone electron-withdrawing end groups, which are supposed to be conformationally interlocked by noncovalent interactions, leading to good molecular planarity. In addition, compact solid state stacking was revealed by UV–vis–NIR absorption spectrum. The optimized PM6:BID-4 F based device delivers an eminent power conversion efficiency(PCE) of 12.30% with a high open-circuit voltage(Voc) of 0.92 V and a high fill factor(FF) of 74.3%. Most importantly, the PCE and FF are among one of the highest values reported for the OSCs based on the unfused-ring SMAs. Overall, our work demonstrates that the unfused ring central framework with high molecular planarity through noncovalent interactions provides a good strategy to construct highly efficient SMAs.

关键词： Diflurobenzothiadiazole core Noncovalently fused small molecule acceptor High efficiency High fill factor