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Application of Wet Waste from Shrimp(Litopenaeus vannamei) with or Without Sea Mud to Feeding Sea Cucumber(Stichopus monotuberculatus)

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Journal of Ocean University of China 2015年第1期14卷 114-120页

作者： CHEN Yanfeng hu chaoqun REN Chunhua Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB) Key Laboratory of Applied Marine Biology of Guangdong Province (LAMB) South China Sea Institute of Oceanology Chinese Academy of Sciences College of Life Science Foshan University

In the present study, the applicability of the wet waste collected from shrimp(Litopenaeus vannamei) to the culture of sea cucumber(Stichopus monotuberculatus) was determined. The effects of dietary wet shrimp waste on the survival, specific growth rate(SGR), fecal production rate(FPR), ammonia- and nitrite-nitrogen productions of sea cucumber were studied. The total organic matter(TOM) level in the feces of sea cucumber was compared with that in corresponding feeds. Diet C(50% wet shrimp waste and 50% sea mud mash) made sea cucumber grow faster than other diets. Sea cucumber fed with either diet D(25% wet shrimp waste and 75% sea mud mash) or sole sea mud exhibited negative growth. The average lowest total FPR of sea cucumber occurred in diet A(wet shrimp waste), and there was no significant difference in total FPR between diet C and diet E(sea mud mash)(P > 0.05). The average ammonia-nitrogen production of sea cucumber in different diet treatments decreased gradually with the decrease of crude protein content in different diets. The average highest nitrite-nitrogen production occurred in diet E treatment, and there was no significant difference in nitrite-nitrogen production among diet A, diet B(75% wet shrimp waste and 25% sea mud mash) and diet C treatments(P > 0.05). In each diet treatment, the total organic matter(TOM) level in feces decreased to different extent compared with that in corresponding feeds.

关键词： sea cucumber Stichopus monotuberculatus shrimp Litopenaeus vannamei wet waste sea mud

达芬奇机器人辅助胸腔镜在小儿纵隔肿瘤切除术中使用的可行性及安全性

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实用医学杂志 2024年第13期40卷 1840-1845页

作者：刘慧芳汪力董璠胡超群陈德美范丽华中科技大学同济医学院附属武汉儿童医院(武汉市妇幼保健院)手术室武汉430000 华中科技大学同济医学院附属武汉儿童医院(武汉市妇幼保健院)心胸外科武汉430000

目的探讨达芬奇机器人辅助胸腔镜在小儿纵隔肿瘤切除术中的应用效果。方法选取进行纵膈肿瘤切除的80例患儿,随机分为两组,各40例。对照组采用普通胸腔镜技术,观察组应用达芬奇机器人辅助胸腔镜。对比两组围术期指标,并根据手术时间和术... 详细信息

目的探讨达芬奇机器人辅助胸腔镜在小儿纵隔肿瘤切除术中的应用效果。方法选取进行纵膈肿瘤切除的80例患儿,随机分为两组,各40例。对照组采用普通胸腔镜技术,观察组应用达芬奇机器人辅助胸腔镜。对比两组围术期指标,并根据手术时间和术中出血量建立学习曲线;比较手术前和术后24 h的疼痛介质水平、应激反应指标、术后并发症;比较术后1个月的炎性因子水平,并通过儿童生活质量普适性核心量表评价患儿术前和术后1个月的生活质量。结果观察组和对照组的手术时间分别为(1.76±0.33)和(2.82±0.62)h,术中出血量分别为(49.83±6.39)和(71.55±8.19)mL,且观察组的术后引流时间、引流量和住院时间均低于对照组(P<0.05)。观察组术后血清缓激肽(BK)、5-羟色胺(5-HT)、神经肽Y(NPY)、前列腺素E2(PGE2)水平分别为(8.06±1.06)mg/L、(170.20±13.21)ng/L、(201.82±13.52)mg/L、(241.82±15.32)ng/L,其疼痛介质水平、应激反应指标和炎性因子水平均低于对照组(P<0.05);观察组和对照组并发症总发生率分别为2.50%和20.00%,且观察组手术后的生活质量比对照组提高,差异有统计学意义(P<0.05)。结论达芬奇机器人辅助胸腔镜在小儿纵隔肿瘤切除术中具有更高的可行性和安全性,值得临床推广。

关键词：达芬奇机器人胸腔镜纵隔肿瘤应激反应

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电沉积石墨烯防腐涂层的研究进展

广州化学 2024年第1期49卷 16-21,I0002页

作者：胡超群赵诗翔李静上海理工大学材料与化学学院上海200093

石墨烯材料的二维平面结构以及优异的抗渗性使其在防腐领域备受关注,电沉积法可以实现石墨烯片层在涂层中的有序排列,增强涂层对外界腐蚀性电解质的阻隔。简单介绍了电沉积法制备石墨烯涂层的原理,详细综述了近年来以电沉积法制备金属... 详细信息

石墨烯材料的二维平面结构以及优异的抗渗性使其在防腐领域备受关注,电沉积法可以实现石墨烯片层在涂层中的有序排列,增强涂层对外界腐蚀性电解质的阻隔。简单介绍了电沉积法制备石墨烯涂层的原理,详细综述了近年来以电沉积法制备金属基石墨烯复合涂层和导电聚合物基石墨烯复合涂层的研究进展,分析了石墨烯改善在不同基质涂层的原理。最后,展望了石墨烯、金属和导电聚合物三元复合材料在防腐涂层中的应用,并且对两种复合涂层的优缺点进行了简单的总结。

关键词：电沉积石墨烯金属导电聚合物复合涂层防腐性能

Characterization of mitochondrial genome of sea cucumber Stichopus horrens:A novel gene arrangement in Holothuroidea

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Science China(Life Sciences) 2011年第5期54卷 434-441页

作者： FAN SiGang hu chaoqun WEN Jing ZHANG LvPing Key Laboratory of Marine Bio-resources Sustainable Utilization Key Laboratory of Applied Marine Biology of Guangdong Province and Chinese Academy of Sciences South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou 510301 China Graduate University of Chinese Academy of Sciences Beijing 100049 China Department of Biology Zhanjiang Normal University Zhanjiang 524048 China

The complete mitochondrial DNA sequence contains useful information for phylogenetic analyses of metazoa. In this study, the complete mitochondrial DNA sequence of sea cucumber Stichopus horrens （Holothuroidea： Sdchopodidae： Stichopus） is presented. The complete sequence was determined using normal and long PCRs. The mitochondrial genome of Stichopus horrens is a circular molecule 16257 bps long, composed of 13 protein-coding genes, two ribosomal RNA genes and 22 transfer RNA genes. Most of these genes are coded on the heavy strand except for one protein-coding gene （had6） and five tRNA genes （tRNAser（UcN）, tRNAGtn, tRNAAla, tRNA val, tRNAASp） which are coded on the light strand. The composition of the heavy strand is 30.8% A, 23.7% C, 16.2% G, and 29.3% T bases （AT skew=0.025; GC skew=-0.188）. A non-coding region of 675 bp was identified as a putative control region because of its location and AT richness. The intergenic spacers range from 1 to 50 bp in size, totaling 227 bp. A total of 25 overlapping nucleotides, ranging from 1 to 10 bp in size, exist among 11 genes. All 13 protein-coding genes are initiated with an ATG. The TAA codon is used as the stop codon in all the protein coding genes ex- cept nad3 and nad4 that use TAG as their termination codon. The most frequently used amino acids are Leu （16.29%）, Ser （10.34%） and Phe （8.37%）. All of the tRNA genes have the potential to fold into typical cloverleaf secondary structures. We also compared the order of the genes in the mitochondrial DNA from the five holothurians that are now available and found a novel gene arrangement in the mitochondrial DNA of Stichopus horrens.

关键词： complete mitochondriai DNA Stichopus horrens gene arrangement Holothuroidea

Effect of Shrimp(Litopenaeus vannamei) Farming Waste on the Growth, Digestion, Ammonium-Nitrogen Excretion of Sea Cucumber(Stichopus monotuberculatus)

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Journal of Ocean University of China 2015年第3期14卷 484-490页

作者： CHEN Yanfeng LUO Peng hu chaoqun REN Chunhua CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB) Key Laboratory of Applied Marine Biology of Guangdong Province and Chinese Academy of Sciences (LAMB) South China Sea Institute of Oceanology Chinese Academy of Sciences College of Life Science Foshan University

In this study, specific growth rate(SGR), ingestion rate(IR), food conversion ratio(FCR), apparent digestion ratio(ADR) and ammonium-nitrogen excretion were determined for sea cucumber(Stichopus monotuberculatus) reared in plastic containers(70 L; 4 containers each diet treatment). Sea cucumbers were fed with five diets containing different amounts of farming waste from shrimp(Litopenaeus vannamei)(100%, 75%, 50%, 25% and 0) and a formulated compound(20% sea mud and 80% powdered algae). Sea cucumbers grew faster when they were fed with diet D(25% shrimp waste and 75% formulated compound) than those fed with other diets. Although IR value of sea cucumber fed with diet A(shrimp waste) was higher than those fed with other diets, both the lowest SGR and the highest FCR occurred in this diet group. The highest and the lowest ADR occurred in diet E(formulated compound) and diet A group, respectively, and the same to ammonium-nitrogen excretion. The contents of crude protein, crude lipid and total organic matter(TOM) in feces decreased in comparison with corresponding diets. In the feces from different diet treatments, the contents of crude protein and TOM increased gradually as the contents of crude protein and TOM in diets increased, while crude lipid content decreased gradually as the crude lipid content in diets increased.

关键词： sea cucumber Stichopus monotuberculatus shrimp Litopenaeus vannamei growth digestion ammonium-nitrogenexcretion

Heterotrophic bacteria of the Dapeng Bay in the South China Sea: community structure,abundance,and the relationships of culturablity with environmental factors

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Acta Oceanologica Sinica 2010年第4期29卷 88-97页

作者： JIANG Fajun hu Zhangli hu chaoqun South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou 510301 China Guangxi Academy of Sciences Nanning 530007 China College of Life Sciences Shenzhen University Shenzhen 518060 China

The community composition,abundance and culturablity of heterotrophic bacterioplankton in the Dapeng Bay of the South China Sea were investigated by morphological method,bacterial cultivation and biochemical methods over an annual *** and Pseudomonas were the predominant genera among the Gram-negative *** bacterioplankton abundance fluctuated from 2.07×10 8 to 2.44×10 9 cells/dm 3 ,and culturable bacteria abundance ranged between 0.1×10 6 and 16.07×10 6 CFU/dm 3 .The Culturablity of heterotrophic bacteria ranged between 0.01% and 1.31%.The relationship between bacterial culturablity and the environmental factors was also studied in different sites of the Dapeng *** the bay,a significantly positive relationship was evidenced between bacterial culturability and temperature,meanswhile bacterial culturability was significantly inversely related with *** positive relationship was shown at the central bay while a negative relationship was observed at the bay mouth and there was no significant correlation at top bay between bacterial culturablity and chlorophyll a of sea water.A positive relationship was found between culturability and DIN all the bay,but DIP showed a significantly negative relationship with bacterial culturability only at the mouth of the bay.

关键词： heterotrophic bacteria culturable bacteria culturablity environmental factors

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河流下多煤层开采安全性及保护技术

绿色矿山

绿色矿山 2024年第1期2卷 1-10页

作者：郭文兵王比比杨伟强胡超群河南理工大学能源科学与工程学院河南焦作454003 煤炭安全生产河南省协同创新中心河南焦作454003

我国新疆地区干旱缺水,艾维尔沟河作为新疆一条具有重要地理特征和环境影响的河流,其对于流经区域的经济、生态都具有重要意义,因此在艾维尔沟河下进行煤炭开采时,既要保证矿井安全也要保证河流的正常使用。采用理论分析、数值模拟等方... 详细信息

我国新疆地区干旱缺水,艾维尔沟河作为新疆一条具有重要地理特征和环境影响的河流,其对于流经区域的经济、生态都具有重要意义,因此在艾维尔沟河下进行煤炭开采时,既要保证矿井安全也要保证河流的正常使用。采用理论分析、数值模拟等方法对河流下多煤层开采安全性及河流保护技术进行研究,通过理论计算得到多煤层开采条件下,覆岩导水裂隙带发育高度(11401工作面为41.9 m、1502工作面为110 m、11401工作面为113.8 m)并绘制剖面示意图;同时,采用数值模拟方法对覆岩导水裂缝发育形态及高度进行确定,最终基于导水裂隙带高度(117.8 m)与最小煤层埋藏深度(191.5 m)的相互关系,确定河流下开采是安全的可行的。在保证河流下煤层安全开采的前提下,通过分析离层注浆原理,并结合采动影响特征参数及工程现场,提出了以离层注浆为主,河道防渗及优化开采设计为辅的多手段对采动影响范围内地表特定目标的精准保护技术。根据实际采矿地质条件,采用概率积分法计算试采工作面开采前后河道位置处地表移动变形,计算结果显示:采用离层注浆精准保护技术后,河道的最大水平变形值(2 mm/m)未超出允许坝体的极限变形值(2.5 mm/m),所以采用离层注浆精准保护技术可以实现,井下安全开采的同时,保证河道的正常使用。通过对河流下多煤层开采的安全性及保护技术的分析研究,为矿山绿色开采提供参考和借鉴。

关键词：绿色开采河流下采煤导水裂隙带高度离层注浆多煤层开采

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模型参数对直喷汽油发动机喷雾特性的影响

同济大学学报（自然科学版） 2024年第9期52卷 1456-1463页

作者：冷鹏飞胡超群杨春雨阮慧琳赵文伯于洋李理光吴志军同济大学汽车学院上海201804

基于单向耦合模拟方法,研究了湍流模型参数、单向耦合模型参数和破碎模型参数对喷雾贯穿距和粒径的影响。结果表明,湍流模型参数C_(ε1)和RT(Rayleigh⁃Taylor)破碎模型尺寸参数CRT对喷雾贯穿距的影响最大,KH(Kelvin⁃Helhmoltz)破碎模型... 详细信息

基于单向耦合模拟方法,研究了湍流模型参数、单向耦合模型参数和破碎模型参数对喷雾贯穿距和粒径的影响。结果表明,湍流模型参数C_(ε1)和RT(Rayleigh⁃Taylor)破碎模型尺寸参数CRT对喷雾贯穿距的影响最大,KH(Kelvin⁃Helhmoltz)破碎模型尺寸参数C_(KH)和C_(RT)对粒径的影响最大。在此基础上建立了适用于不同工况的高精度喷雾模型,标准Spray G工况下,模拟结果与实验结果误差在3%以内;不同环境气体密度下,多种工况的模拟结果与实验结果误差在10%左右。

关键词：发动机模型参数贯穿距粒径

All-trans retinoic acid reverses malignant biological behavior of hepatocarcinoma cells by regulating miR-200 family members

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Genes & Diseases 2021年第4期8卷 509-520页

作者： Jiejie Cui Mengjia Gong Shuyu Fang chaoqun hu Yi Wang Jingfang Zhang Ni Tang Yun He Department of Pediatric Surgery Ministry of Education Key Laboratory of Child Development and DisordersNational Clinical Research Center for Child Health and DisordersChina International Science and Technology Cooperation Base of Child Development and Critical DisordersChongqing Key Laboratory of PediatricsChildren’s Hospital of Chongqing Medical UniversityChongqing401122PR China Puyang People’s Hospital PuyangHenan Province457000PR China

As a potential chemo-therapeutic agent,all-trans retinoic acid(ATRA)can signif-icantly reverse epithelial-mesenchymal transition(EMT)of hepal-6 hepatocarcinoma cell line in vitro,but the mechanism is *** expression profile of microRNA-200(miR-200)families is different in hepatocellular *** this study,we found that ATRA trea tment could up-regulate the expression of miR-200a-3p,200c-3p,and 141-3p,which were involved in ATRA regulated proliferation and apoptosis of hepal-6 cell,but not colony ***,miR-200a-3p,200c-3p,and 141-3p could recovery ATRA inhibited migration and invasion abilities of hepal-6 cells at various ***-200a-3p and 200c-3p prevented ATRA from inducing the differentia tion and hepatic functions of hepal-6 ***-tagomir specific for miR-200a-3p and 200c-3p down-regulated the expression of CK18,but only miR-200a-3p anta gomir played prominent role in regula ting the expression of these mesenchymal markers,N-Cadherin,Snail and *** transcriptional activities of 8 tran-scription factors were up-regulated and 35 transcription factors were down-regulated by *** with ATRA group,inhibition of miR-200a-3p,200c-3p,and 141-3p significantly strengthened the expression of Fra1/Jun(AP1),Ets1/PEA3,Brn3,and Zeb1/AREB6 at varying ***,this result suggested that ATRA may suppress EMT through down-regulating miR-200a-3p,200c-3p and 141-3p related transcription ***-200 and their downstream genes might be the potentially specific targets for the treat-ment of hepatocarcinoma.

关键词： All-trans retinoic acid Differentiation Epithelial emesenchymal transition Hepatocellular carcinoma cells microRNA 200