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Germline cells derived from mesenchymal stem cells, with the focus on Wharton's jelly

Asian pacific Journal of Reproduction 2018年第2期7卷 49-55页

作者： hossein yazdekhasti Jalil hosseini Zahra Rajabi Maryam hosseinzadeh Shirzeyli Fereshte Aliakbari Department of Cell Biology Center for Research in Contraceptive and Reproductive HealthUniversity of VirginiaCharlottesvilleVA22908USA Infertility and Reproductive Health Research Center Shahid Beheshti University of Medical SciencesTehranIran Department of Anatomy School of MedicineTehran University of Medical SciencesTehranIran Department of Biomedical Engineering University of VirginiaCharlottesvilleVA22908USA Department of Biology and Anatomical Sciences School of MedicineShahid Beheshti University of Medical SciencesTehranIran

Previous attempts have indicated that mesenchymal stem cells (MSCs) are a valuable source and candidate and new approach for tissue engineering and reproductive medicine. MSCs have this potential to be induced and differentiated in an appropriatein vivoandin vitro condition toward various cell lineages and then they can be applied in cell therapies and clinical applications. During recent two decades, various sources have demonstrated they are a great source for MSCs, including bone marrow, the human umbilical cord as well as Wharton's jelly. Due to discarding after birth, easily accessible cells and less ethical concerns, these cells have attracted more and more scientists' attention. Infertility and reproduction diseases have provided special opportunity to examine the efficiency of MSCs in this kind of application. Based on recent investigations, MSCs embedded in Wharton's jelly tissue are more appealing for cell therapies, especially in infertility treatment purposes. So, differentiation of MSCs embedded in Wharton's jelly tissue into germ layer cells for cell-based therapy purposes is now under intensive study.

关键词： Mesenchymal stem cell Bone marrow stem cell Umbilical cord Wharton's jelly

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Effects of vascular endothelial growth factor supplementation and alginate embedding on human oocyte maturation in vitro

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Asian pacific Journal of Reproduction 2020年第3期9卷 129-134页

作者： Farhang Abed Morteza Fallah-Karkan Masoumeh Majidi Zolbin Pegah Naghizadeh Fereshte Aliakbari hossein yazdekhasti Men's Health&Reproductive Health Research Center Shahid Beheshti University of Medical SciencesTehranIran Laser Application in Medical Science Research Center Shahid Beheshti University of Medical SciencesTehranIran Shohada-e-Tajrish Hospital Shahid Beheshti University of Medical SciencesTehranIran Pediatric Urology and Regenerative Medicine Research Center Children's Medical CenterTehran University of MedicalTehranIran Department of Anatomy Faculty of MedicineTehran University of Medical SciencesTehranIran Department of Molecular Physiology and Biological Physics Center for Membrane and Cell PhysiologyUniversity of VirginiaVirginiaUnited States

Objective:To evaluate whether the use of vascular endothelial growth factor(VEGF)with alginate increases oocyte maturation following in vitro ***:This experimental study was performed on 150 immature oocytes(germinal vesicle oocytes)from females who were candidates for assisted reproductive *** germinal vesicle oocytes were randomly placed in the control,alginate,and VEGF plus alginate *** basic culture medium for oocytes culture(tissue culture medium 199,follicle-stimulating hormone 0.075 IU/mL,and fetal bovine serum 10%)was used in the control,alginate,and VEGF plus alginate *** the treatment groups(alginate,and VEGF plus alginate groups),alginate(8%)and VEGF(5 ng/mL)were added to the basic culture *** culture,immature oocytes were considered as oocytes unchanged in the nucleus whereas oocytes with a polar body were considered as mature oocytes(metaphaseⅡstage).The mature oocytes in each group were fertilized by intracytoplasmic sperm injection and formed embryos were evaluated by reverse ***:The oocyte maturation rate(metaphaseⅡ)significantly(P<0.05)increased in the alginate plus VEGF group as compared with the alginate alone and control groups during in vitro *** day 2,the cleavage rates were significantly different in the matured oocytes between the treatment groups and the control *** percentage of the two-cell stage,four-cell stage and eight-cell embryos was significantly higher in the treatment groups compared with the control group(P<0.05).Conclusions:Supplementation of VEGF with alginate can improve oocyte maturation in culture *** with alginate may promote the quality of nuclear and cytoplasmic maturation of human oocytes in vitro.

关键词： Alginate Infertility Human oocyte Maturation Vascular endothelial growth factor

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