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Amino acid sequence of BmK AS, a novel polypeptide activator of ryanodine receptor on skeletal muscle

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Chinese Science Bulletin 1997年第11期42卷 952-956页

作者： Ji, YH Liu, Y Xu, K Ohishi, T Mochizuki, T hoshino, M Yanaihara, N Shanghai Institute of Physiology Chinese Academy of Sciences Shanghai China School of Pharmaceutical Sciences University of Shizuoka Shizuoka Japan Yanaihara 1st. Inc. Fujinomiya-shi Japan

IN ref.[1],we have described a novel activator(BmK AS)of ryanodine receptor on skeletal muscle,which was obtained from the venom of scorpion Buthus martensi *** noteprovides the complete amino acid sequence of the active polypeptide,BmK *** molecularweight ot the polypeptide thus determined was 7 698 u,calculated based on its sequence(to-tally 66 residues),which was coincident with the value of 7 696.26 u determined by electro-

关键词： scorpion Buthus martensi Karsch ryanodine receptor activator amino acid sequence.

Shedding new light on the dislocation-mediated plasticity in wurtzite ZnO single crystals by photoindentation

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Journal of Materials Science & Technology 2023年第25期156卷 206-216页

作者： Yan Li Xufei Fang Eita Tochigi Yu Oshima Sena hoshino Takazumi Tanaka Hiroto Oguri Shigenobu Ogata Yuichi Ikuhara Katsuyuki Matsunaga Atsutomo Nakamura Department of Mechanical Science and Bioengineering Osaka University1-3 Machikaneyama-choToyonaka 560-8531Japan Department of Materials and Earth Sciences Technical University of Darmstadt64287 DarmstadtGermany Institute of Industrial Science The University of Tokyo4-6-1 KomabaMeguro-kuTokyo 153-8505Japan PRESTO Japan Science and Technology Agency (JST)4-1-8HonchoKawaguchi-shiSaitama 332-0012Japan Department of Materials Physics Nagoya UniversityFuro-choChikusa-kuNagoya 464-8603Japan Institute of Engineering Innovation The University of Tokyo2-11-16 YayoiBunkyo-kuTokyo 113-8656Japan

Dislocation-mediated plasticity in inorganic semiconductors and oxides has attracted increasing research interest because of the promising mechanical and functional properties tuned by *** this study,we investigated the effects of light illumination on the dislocation-mediated plasticity in hexagonal wurtzite ZnO,a representative third-generation semiconductor material.A(0001)45o off sample was specially designed to preferentially activate the basal slip on(0001)*** types of nanoindentation tests were performed under four different light conditions(550 nm,334 nm,405 nm,and darkness),including low-load(60μN)pop-in tests,high-load(500μN)nanoindentation tests,and nanoindentation creep *** maximum shear stresses at pop-in were found to approximate the theoretical shear strength regardless of the light *** activation volume at pop-ins was calculated to be larger in light than in ***-sectional transmission electron microscope images taken from beneath the indentation imprints showed that all indentation-induced dislocations were located beneath the indentation imprint in a thin-plate shape along one basal slip *** indentation-induced dislocations could spread much deeper in darkness than in light,revealing the suppressive effect of light on dislocation *** analytical model was adopted to estimate the elastoplastic stress field beneath the *** was found that dislocation glide ceased at a higher stress level in light,indicating the increase in the Peierls barrier under light ***,nanoindentation creep tests showed the suppression of both indentation depth and creep rate by *** creep also yielded a larger activation volume in light than in darkness.

关键词： Photoindentation Compound semiconductors Oxides Crystal plasticity Dislocations

Novel diagnosis and therapy for hepatoma targeting HBV-related carcinogenesis through alternative splicing of FIR (PUF60)/FIRΔexon2

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Hepatoma Research 2018年第9期4卷 142-158页

作者： Kazuyuki Matsushita Tyuji hoshino Department of Laboratory Medicine&Division of Clinical Genetics and Proteomics Chiba University HospitalChiba260-8677Japan Department of Physical Chemistry Chiba UniversityChiba 260-8670Japan

Aim: Disturbed alternative splicing of far upstream element-binding protein-interacting repressor (FIR) was found to be unable to repress c-Myc transcription and so it might be important for suppressing tumor development. FIR is a splicing variant of poly (U)-binding-splicing factor (PUF60), and forms complex with other splicing factors. FIR/PUF60 is a splicing factor of U2 small nuclear ribonucleoprotein auxiliary factor family, Thus FIR/PUF60 is a multifunctional protein. The expression of exon2-lacking splicing variant of FIR, FIRΔexon2, is elevated in many cancer tissues and promotes tumor development by disabling FIR-repression to sustain c-Myc activation. FIRΔexon2, as a dominant negative of FIR, opposed apoptosis in cancer cells. FIR/FIRΔexon2 interacts with degron pocket of F-box and W (Typ) D (Asp) repeat domain-containing 7 and inhibits proteolysis of substrates proteins. Recently, FIR/PUF60 was identified as a versatile regulator of transcriptional and post-transcriptional steps in expression of hepatitis B virus (HBV) pregenomic RNA (pgRNA) expression. Methods: Small molecular chemical compounds against FIR and FIRΔexon2 were screened among 2,3275 chemicals by natural product depository array (RIKEN, Wako, Saitama, Japan). Results: Nine chemicals against FIR and four chemicals against FIRΔexon2 were identified as candidates of interacting chemicals. Interestingly, BK697 contains WD -like structure. Among them, BK697 against FIRΔexon2 inhibited hepatoma cell growth. Conclusion: Therefore, FIR (PUF60)/FIRΔexon2 is multifunctional and applicable for clinical use for HBV suppression and hepatoma treatment. Together, one clue to the development of hepatome diagnosis and therapies directed against FIR/FIRΔexon2/PUF60 with small molecular weight chemicals that inhibit HBV cccDNA replication.

关键词： Hepatocellular carcinoma hepatitis B virus covalently closed circular DNA far upstream element-binding protein-interacting repressor poly (U)-binding-splicing factor F-box and W (Typ) D (Asp) repeat domain-containing 7 natural product depository array U2A

Colonization of neonate mouse spermatogonial stem cells co-culture with Sertoli cells in the presence and absence soft agar

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Asian pacific Journal of Reproduction 2020年第6期9卷 298-304页

作者： Ali Talebi Shadan Navid Maryam Borhani-Haghighi Yumi hoshino Mehdi Abbasi Zahra Khosravizadeh School of Medicine Shahroud University of Medical SciencesShahroudIran Tissue Engineering and Stem Cells Research Center Shahroud University of Medical SciencesShahroudIran Sexual Health and Fertility Research Center Shahroud University of Medical SciencesShahroudIran Department of Anatomy Gonabad University of Medical SciencesGonabadIran Department of Anatomy School of MedicineTehran University of Medical SciencesTehranIran Laboratory of Reproductive Endocrinology Graduate School of Biosphere ScienceHiroshima UniversityHiroshimaJapan

Objective:To investigate the effects of soft agar on in-vitro proliferation of neonate mouse spermatogonial stem cells co-cultured with Sertoli ***:Tissues of neonate NMRI male mice testes were used for harvesting spermatogonial stem cells and Sertoli *** cell harvest,flow cytometry using promyelocytic leukemia zinc-finger(PLZF)protein antibody was used to assess the purity of the *** isolated testicular cells were cultured in the absence(the control group)or presence of soft agar-coated dishes(the experimental group)supplemented with leukemia inhibitory factor and glia cell line–derived neurotrophic factor for two *** phosphatase activity was assessed in the colonies formed after two weeks of culture by alkaline phosphatase *** day 14 of culture,the expression levels of DNA-binding protein inhibitor(ID-4)and PLZF genes in the undifferentiated cells were evaluated by the detection of PLZF protein antibody using real-time PCR and immunocytochemistry *** number and diameter of the colonies of spermatogonial stem cells were assessed by ImageJ ***:In the experimental group,the number and the diameter of colonies significantly increased as compared with those in the control group(P<0.05,P<0.01,respectively).In addition,the level of expression of ID-4 and PLZF genes in the undifferentiated cells significantly increased in the experimental group as compared with the control group(P<0.01).However,the expression of tyrosine-protein kinase kit(c-kit)gene in differentiated cells decreased in the experimental group as compared with the control group,but there was no significant difference between the two ***:Spermatogonial stem cells can be efficiently proliferated by culturing the stem cells in soft agar-coated *** new protocol used in this study can be a valuable method for future studies.

关键词： Spermatogonial stem cell Soft agar Colonization Proliferation Mouse

Modulatory Effect of Motivation on the Association of Trait Anxiety and Cognitive Performance: A Pupillometric Study

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Journal of Behavioral and Brain Science 2017年第7期7卷 273-286页

作者： Takatoshi hoshino Yoshihiko Tanno Department of Psychoinformatics Kanazawa Institute of Technology Ishikawa Japan Graduate School of Arts and Sciences The University of Tokyo Tokyo Japan

The attentional control theory (ACT) proposes that trait anxiety disrupts functional efficiency of executive control of attention relating to working memory system such as shifting. ACT also emphasizes the modulatory role of motivation in anxiety on cognitive task performance. The present study investigated the association of trait anxiety-related inefficiencies in attentional shift and working memory performance in conjunction with the level of motivation. A variation of complex span paradigm is designed to systematically manipulate the time constraint on shifting attentional focus back and forth between working memory contents and processing task was used in this study. In the experiment, participants high and low in trait anxiety were allocated either high or low motivation induction conditions, and performed a series of complex span tasks. They also completed a state anxiety measure before and after the experimental task. Motivational states were assessed by the pupil dilation, which is known to reflect the amount of cognitive effort invested on the task at hand. Results showed that, only in low motivation condition, high trait-anxious individuals exhibited greater difficulty, relative to those low in trait anxiety, in maintaining working memory contents as the task demand increased. State anxiety showed no relation to working memory performance regardless of the level of motivation and task demands. Pupillary responses revealed that, in high motivational state, high trait-anxious individuals invested more effort than those low in trait anxiety when the task demand was low, F(1, 132) = 6.65, p = 0.0110, to moderate, F(1, 132) = 8.441, p = 0.00043. In accordance with ACT, these findings suggest that motivation can modulate the association between trait anxiety and cognitive performance along with the levels of task demand. Clinical implication is briefly discussed.

关键词： Trait Anxiety Motivation Working Memory Attentional Control Theory (ACT) Pupillometry

Bioactive polymeric scaffolds for tissue engineering

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Bioactive Materials 2016年第2期1卷 93-108页

作者： Scott Stratton Namdev B.Shelke Kazunori hoshino Swetha Rudraiah Sangamesh G.Kumbar Department of Orthopaedic Surgery UConn HealthFarmingtonCTUSA Institute for Regenerative Engineering UConn HealthFarmingtonCTUSA Department of Biomedical Engineering University of ConnecticutStorrsCTUSA Department of Pharmaceutical Sciences School of PharmacyUniversity of Saint JosephHartfordCT06103USA

A variety of engineered scaffolds have been created for tissue engineering using polymers,ceramics and their *** has been adopted for majority of the three-dimensional(3D)scaffold design both in terms of physicochemical properties,as well as bioactivity for superior tissue *** fabricated via salt leaching,particle sintering,hydrogels and lithography have been successful in promoting cell growth in vitro and tissue regeneration in *** systems derived from decellularization of whole organs or tissues has been popular due to their assured biocompatibility and *** scaffold fabrication techniques often failed to create intricate structures with greater resolution,not reproducible and involved multiple *** 3D printing technology overcome several limitations of the traditional techniques and made it easier to adopt several thermoplastics and hydrogels to create micro-nanostructured scaffolds and devices for tissue engineering and drug *** review highlights scaffold fabrication methodologies with a focus on optimizing scaffold performance through the matrix pores,bioactivity and degradation rate to enable tissue *** highlights few examples of bioactive scaffold mediated nerve,muscle,tendon/ligament and bone *** of the efforts required for optimization,a shift in 3D scaffold uses from the laboratory into everyday life is expected in the near future as some of the methods discussed in this review become more streamlined.

关键词： Bioactive Biomaterials Scaffold Porosity Biodegradable Tissue regeneration

基于混合方法的PWM逆变器下永磁同步电动机的温升研究

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电机与控制应用 2009年第4期36卷 1-5页

作者：李虎 Akihiro hoshino 清华大学电机系北京100084 日本三菱重工名古屋研究所名古屋4538515

提出一种新型基于等效热网络与有限元相结合的混合方法,建立了三维磁热耦合模型,对一台在脉宽调制(PWM)逆变器驱动下的30 kW/50 Hz表贴式永磁同步电动机进行了仿真研究。首先用场路紧密耦合方法分析电机的分布热源,包括涡流损耗、铁损... 详细信息

提出一种新型基于等效热网络与有限元相结合的混合方法,建立了三维磁热耦合模型,对一台在脉宽调制(PWM)逆变器驱动下的30 kW/50 Hz表贴式永磁同步电动机进行了仿真研究。首先用场路紧密耦合方法分析电机的分布热源,包括涡流损耗、铁损和铜损。利用该热源分布,结合适当的热网络,研究了耦合温升问题。仿真结果表明,由于载波谐波的影响导致永磁体中涡流损耗的大量增加,其温升也非常显著。试验验证表明,磁块表面和机壳的测量温度与分析结果相吻合,因此该分析方法能准确预测永磁体温度。

关键词：永磁同步电动机温升场路紧密耦合

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Chinese New Year

小学生时代（大嘴英语） 2023年第1期 19-22页

作者： Jacqueline Schif Felicia hoshino(图) 不详

Sweep the bad luck out ***,clean,clean the kitchen *** and tidy every room,Chinese New Year's coming soon.

关键词： Clean floor clean

PWM逆变器下永磁同步电动机中永磁体的三维涡流分析*

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电机与控制应用 2009年第8期36卷 1-5页

作者：李虎 Akihiro hoshino 清华大学电机工程与应用电子技术系北京100084 日本三菱重工名古屋研究所名古屋4538515

对于永磁同步电动机中永磁体的发热及温升,快速准确地分析永磁体的涡流损耗是其关键问题所在。用场路紧密耦合方法,建立了考虑永磁体分割的三维有限元模型,对一台在脉宽调制(PWM)逆变器驱动下的30kW/50Hz表贴式永磁同步电动机进行仿真研... 详细信息

对于永磁同步电动机中永磁体的发热及温升,快速准确地分析永磁体的涡流损耗是其关键问题所在。用场路紧密耦合方法,建立了考虑永磁体分割的三维有限元模型,对一台在脉宽调制(PWM)逆变器驱动下的30kW/50Hz表贴式永磁同步电动机进行仿真研究,分析了其气隙旋转磁场、电枢电流,以及在不同载波频率下永磁体的涡流损耗。研究表明:在载波谐波作用下,永磁体的涡流损耗比在正弦电压驱动下大幅增加,并将引起更大的温升。输入电流波形、转矩和功率平衡关系等试验结果表明,该分析方法能准确计算出永磁体的涡流损耗,为下一步的温升研究奠定了基础。

关键词：永磁同步电动机涡流损耗紧密场路耦合