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A Study on the Fish Diversity of Sanhuanpao Wetland in Heilongjiang Province

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Asian Agricultural Research 2017年第10期9卷 68-71页

作者： Xinxin WANG Fangying CHAI hongxian yu Northeast Forestry University Harbin 150040China Heilongjiang Provincial Department of Water Resources Harbin 150040China

The current situation of fish in Sanhuanpao Wetland was investigated for the first time in this paper. In May( Spring),July( summer),September( autumn) 2016,it was sampled three times for investigation,and based on the investigation data about the fish caught by net45 times in 9 sampling points,the species composition of fish community in the water was analyzed. The results showed that a total of 12 fish species were caught in this investigation,belonging to 4 orders,6 families and 12 genera. There were five fish species for Cyprinidae,accounting for 41. 7% of total species,followed by Cobitidae with three species,accounting for 25% of total species; Siluridae,Esocidae,Eleotridae and Channidae each had 1 species,accounting for 8. 3% of total species. The fish with index of relative importance( IRI) greater than 1000 is the dominant species,and it was calculated that the dominant species were Carassius auratus gibelio,Rhynchocypris percnurus,Misgurnus mohoity,Cobitis taenia Linnaeus,and Perccottus glenii. In this paper,based on the number of individuals,by calculating Margalcf abundance index( D),Shannon-Wiener diversity index( H') and Pielou evenness index( J') for the fish in the water,the current situation of fish resources in Sanhuanpao Wetland was analyzed and the corresponding protection recommendations were put forward.

关键词： Sanhuanpao Wetland Fish Diversity

Current Development and Prospects of Pond Smelt （Hypomesus olidus） Farming Industry in China

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Asian Agricultural Research 2018年第11期10卷 62-67页

作者： Qingyu CHAI Fangying CHAI Ting yu Yongfu JU hongxian yu Northeast Forestry University Harbin 150000 China Heilongjiang Provincial Water Resources Department Harbin 150000 China

The pond smell (Hypomesus olidus) , originally transplanted from North Korea, was introduced to China in 1938 and gradually distributed to various areas of China. The pond smelt was the first fish species inlnxluced to the water of China. It not only has the function of protecting water quality, and also has high economic and nutritional values which are helpful for diversifying the fishery productive activities of China. The present review analyzed the transplantation and production status of pond smelt in China, deseribed the key situations and factors influencing the establishment and current level of aquaculture development. Since its introduction, the pond smelt transplantation in China was distributed in Northeast of China. From the 1980s, reservoirs and lakes all over China began to transplant pond smelt, and the transplantation of pond smelt went into the outbreak period. In order to improve the efficiency of transplantation, scientific and technical personnel studied the simple and practical technology of artificial breeding and transplantation, the medicine for preventing and controlling sapmlegniasis which often leads to death of fertilized eggs. To meet the demand of market, more and more pond smelt processing products have been studied and developed, such as grilled, milk flavor, fish sauce, ham-sausage, and brew vinegar of pond smelt. The pond smelt farming in China has broad prospects development, yet there are also challenges to overcome. It is recommended to take proper policies for pond smelt development, strengthen pond smelt scientific research, transplantation technology training, establish pond smelt fisheries association, support dominant enterprises, and implement brand strategy. [ABSTRACT FROM AUTHOR]

关键词：池塘人工育种农业种植技术

A new photolabeling probe for efficient enrichment and deep profiling of cell surface membrane proteome by mass spectrometry

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Chinese Chemical Letters 2023年第2期34卷 279-283页

作者： yuanyuan Li Chaoshuang Xia hongxian Zhao yuping Xie Yangjun Zhang Wanjun Zhang Yongliang yu Jianhua Wang Weijie Qin Research Center for Analytical Sciences Department of ChemistryCollege of SciencesNortheastern UniversityShenyang 110819China National Center for Protein Sciences Beijing State Key Laboratory of ProteomicsBeijing Proteome Research CenterBeijing Institute of LifeomicsBeijing 102206China College of Basic Medicine Anhui Medical UniversityHefei 230032China

The cell surface membrane proteome is a class of proteins encoded by ~25% of all protein-coding genes in living organisms and plays a key role in mediating communication between the cells and their surrounding environment. However, most cell surface membrane proteins(CSMPs) are naturally expressed at very low levels compared with intracellular proteins. The difficulties in their purification with high specificity further hinder the understanding of their structure and function. In this study, we developed a new photolabeling probe to achieve efficient tagging and facile enrichment of the CSMPs. The probe is composed of a lipid tail for cell surface localization, a polyethylene glycol(PEG) spacer for increased water solubility, two 4-(N-maleimido)benzophenone(MBP) groups for UV-active tagging of the CSMPs, and a biotin tag for subsequent isolation. Application of this photolabeling probe resulted in the successful enrichment and identification of 3098 annotated CSMPs in HT22 cells with close to 70% selectivity. The proposed photolabeling probe and enrichment strategy were demonstrated to be a powerful method for deep cell surface proteome profiling, representing one of the largest groups of current drug targets.

关键词： Cell surface membrane proteins Photolabeling probe Enrichment Mass-spectrometry Proteomics Derivatization

Haze Control by Industrialization:A Win-win Mode of Ecological Civilization and Economic Development

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Asian Agricultural Research 2017年第2期9卷 66-71页

作者： Yan HAN Qingyu CHAI Lei WANG yunlong YAO yunna JIA Jiahui SHAO hongxian yu Fangying CHAI Shunde Entry-Exit Inspection and Quarantine Bureau Northeast Forestry University Heilongjiang University of Science and Technology Department of Water Resources of Heilongjiang Province

Haze control is a difficult and arduous battle,and it is a major decision concerning the people's livelihood and national ecological civilization construction.Taking Heilongjiang Province as an example,this paper introduced a new idea for haze control.Haze in Heilongjiang Province was mainly resulted from straw burning.Market-oriented,large-scale,and industrialized haze control relying on science and technology is new opportunity and challenge for realizing ecological civilization and revitalizing the economy of Heilongjiang Province.

关键词： Haze Control Industrialization

非常规储层微纳米孔隙介质中流体相态研究进展

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世界石油工业 2024年第3期31卷 68-77页

作者：汪周华范琨鹏赵建飞余曹匡红先郭平涂汉敏西南石油大学油气藏地质及开发工程重点实验室四川成都610500 大庆油田成都勘探开发研究院四川成都610500

非常规油气储层具有岩石骨架颗粒小、比表面积大、微纳米孔隙发育的特点,流体与孔隙介质间存在着诸多复杂的界面效应问题,影响微纳米孔隙介质中流体的相态变化规律。对于受限域流体,经典油气相态理论方法忽略分子间相互作用及界面效应... 详细信息

非常规油气储层具有岩石骨架颗粒小、比表面积大、微纳米孔隙发育的特点,流体与孔隙介质间存在着诸多复杂的界面效应问题,影响微纳米孔隙介质中流体的相态变化规律。对于受限域流体,经典油气相态理论方法忽略分子间相互作用及界面效应的影响,预测微纳米孔隙介质中流体相变特征存在偏差。为了更全面地揭示油气在受限空间中的相态特征及其微观作用机理,总结当前微纳米孔隙介质中流体相态研究技术现状,详细阐述微观实验测试、相态理论模型和分子模拟3种主流技术方法,指出3种技术各自的优缺点,并对未来流体相态研究技术提出展望。分析认为:微观实验测试方法可通过研究流体热力学参数的变化来确定流体相变点,由于实验误差、人为误差等原因,需与理论计算相结合;相态理论模型方法可通过改进临界参数场、考虑流体与孔壁界面作用、考虑相间毛管压力修正相平衡判据方程等方法建立纳米孔隙空间相态理论模型,但是由于没有综合考虑界面效应带来的影响且缺乏有力的实验数据支撑,难以推广应用;分子模拟方法能在分子尺度上揭示流体分子的相行为,但现有研究主要围绕小分子展开,并且与流体发生相变时的临界参数结合得较少。后续研究应以多界面效应耦合影响的微纳米孔隙介质中油气流体相变特征为研究对象,以微观实验、理论模型和分子模拟为技术手段,为描述微纳米孔隙介质中流体相态提供合理的实验测试手段和可靠的理论计算模型。

关键词：流体相态微观实验测试相态理论模型分子模拟油气藏开发

Hemicellulose in corn straw:Extracted from alkali solution and produced5-hydroxymethyl furfural in HCOOH/HCOONa buffer solution

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Chinese Journal of Chemical Engineering 2016年第12期24卷 1786-1792页

作者： Yan Li hongxian Fan Xueqing yu Songmei Zhang Gang Li Hebei Provincial Key Lab of Green Chemical Technology and High Efficient Energy Saving School of Chemical Engineering and Technology Hebei University of Technology

Hemicellulose in corn straw is a group of complex heteropolysaccharides which are composed of different sugar units, including mannans, xylans, arabinans and galactans. This study developed a simple and practical process for production of 5-hydroxymethyl furfural(HMF) using hemicellulose that was extracted from corn straw. In the hemicellulose degradation process HCOOH/HCOONa was used as buffer solution, and the optimum conditions for maximum HMF yield were explored. Various extraction conditions including NaOH concentration, reaction time,temperature, solid-to-liquid ratio and precipitant were tested for hemicellulose obtaining, giving the optimum condition of 55 °C, 4 h, solid-to-liquid ratio of 1:10, 1.5 mol·L^(-1) NaOH solution and ethanol as precipitant with the yield of 34.16%. Dehydration of hemicellulose under HCOOH/HCOONa buffer solution process, using solution medium of pH = 0.8 hydrolyzed hemicellulose in corn straw at 190 °C after 190 min and 82% of HMF yield was achieved.

关键词： Hemicellulose HMF 降级缓冲区答案 ELSD

Protective effect of Angelica sinensis on cerebral neurons from rat embryos under hypoxia

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Neural Regeneration Research 2007年第1期2卷 46-49页

作者： yuling Wu hongxian Zhao Hong yu Department of Histology and Embryology Luzhou Medical College Luzhou 646000 Sichuan Province China

BACKGROUND： The enhanced expression of c-Fos protein in nerve cells after hypoxia is the marker for converting extracellular hypoxia information to intracellular changes at hypoxia, and it is suspected that the increase of c-Fos protein can lead to the synthesis and excretion of related neurotrophic factor and nerve growth factor. However, it is still unclear what functional changes of nerve cells are induced by the increase of c-Fos protein at hypoxia, and whether it is good for the survival of damaged neurons. OBJECTIVE： To observe the expression of c-Fos in the cerebral neurons from embryos of rats with hypoxia in uterus, and investigate the pathway for the protective effect of Angelica sinensis injection on the cerebral neurons from rat embryos under hypoxia. DESIGN： A completely randomized controlled study. SETTING： Department of Histology and Embryology, Luzhou Medical College. MATERIALS： Twelve female Wistar rats in oestrum and 1 male adult Wistar rat with body mass of 220 to 250 g were selected. Rabbit-anti-rat neuro-specific enolase （NSE） and rabbit-anti-rat c-Fos were purchased from Wuhan Boster Biological Technology Co., Ltd.; Double-staining kit was bought from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. Angelica sinensis injection was produced by the Department of Pharmacy, the Second Affiliated Hospital of Hubei Medical University. METHODS： The experiments were completed in the experimental animal center and the Department of Histology and Embryology of Luzhou Medical College from December 2004 to December 2005. ①Twelve adult female Wistar rats in oestrum and 1 male Wistar rat were housed in one rearing cage. The appearance of vaginal embolus at 8：00 in the next morning was recorded as 0 day of pregnancy and the rats were recorded for 15 days, and they were divided randomly into three groups, control group （n =4）, hypoxia group （n =4） and Angelica group （n =4）. The pregnant rats in the hypoxia group were firstly injected with saline （8 mL/kg）, then put into 2 L wide-mouthed bottle contai

关键词： Angelica sinensis hypoxia neurons

Nonalcoholic steatohepatitis critically rewires the ischemia/ reperfusion-induced dysregulation of cardiolipins and sphingolipids in mice

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Hepatobiliary Surgery and Nutrition 2023年第1期12卷 3-19,I0001,I0002页

作者： Sheng yu Kai Wang Qingping Li Yiran Wei Yiyi Li Qifan Zhang Pengxiang Huang Hanbiao Liang Hang Sun hongxian Peng Xixin Huang Cuiting Liu Jie Zhou Jianping Qian Chuanjiang Li Division of Hepatobiliopancreatic Surgery Department of General SurgeryNanfang HospitalSouthern Medical UniversityGuangzhouChina The First Clinical College Southern Medical UniversityGuangzhouChina Department of Radiation Oncology Nanfang HospitalSouthern Medical UniversityGuangzhouChina Central Laboratory Southern Medical UniversityGuangzhouChina

Background:Lipid dysregulation plays a fundamental role in nonalcoholic steatohepatitis(NASH),which is an emerging critical risk factor that aggravates hepatic ischemia/reperfusion(I/R)injury.However,the specific lipids that mediate the aggressive I/R injury in NASH livers have not yet been identified.Methods:The mouse model of hepatic I/R injury on NASH was established on C56B/6J mice by first feeding the mice with a Western-style diet to induce NASH,then the NASH mice were subjected to surgical procedures to induce hepatic I/R injury.Untargeted lipidomics were performed to determine hepatic lipids in NASH livers with I/R injury through ultra-high performance liquid chromatography coupled with mass spectrometry.The pathology associated with the dysregulated lipids was examined.Results:Lipidomics analyses identified cardiolipins(CL)and sphingolipids(SL),including ceramides(CER),glycosphingolipids,sphingosines,and sphingomyelins,as the most relevant lipid classes that characterized the lipid dysregulation in NASH livers with I/R injury.CER were increased in normal livers with I/R injury,and the I/R-induced increase of CER was further augmented in NASH livers.Metabolic pathway analysis revealed that the enzymes involved in the synthesis and degradation of CER were highly upregulated in NASH livers with I/R injury,including serine palmitoyltransferase 3(Sptlc3),ceramide synthase 2(Cers2),neutral sphingomyelinase 2(Smpd3),and glucosylceramidase beta 2(Gba2)that produced CER,and alkaline ceramidase 2(Acer2),alkaline ceramidase 3(Acer3),sphingosine kinase 1(Sphk1),sphingosine-1-phosphate lyase(Sgpl1),and sphingosine-1-phosphate phosphatase 1(Sgpp1)that catalyzed the degradation of CER.CL were not affected by I/R challenge in normal livers,but CL was dramatically reduced in NASH livers with I/R injury.Consistently,metabolic pathway analyses revealed that the enzymes catalyzing the generation of CL were downregulated in NASH-I/R injury,including cardiolipin synthase(Crls1)and tafazzin(Taz).Notably,the I/R-induced oxidati

关键词： Nonalcoholic steatohepatitis(NASH) ischemia/reperfusion injury lipidomics lipid metabolism

Expression of c-Fos protein and nitricoxide synthase in neurons of cerebral cortex from fetal rats in hypoxia and protective role of Angelica sinensis

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Neural Regeneration Research 2006年第1期1卷 74-77页

作者： Hong yu hongxian Zhao yuling Wu Department of Histology and Embryology Luzhou Medical Collegeof Sichuan Province Luzhou 646000 Sichuan Province China

BACKGROUND： Both c-Fos protein and nitricoxide synthase （NOS） have been used as general indexes in relative research about neurons, but it is lack of reports that c-Fos protein and NOS are applied synchronously to study the neurons of hypoxic fetal rats in uterus. OBJECTIVE： To study the effect of hypoxia in uterus on the expression of c-Fos protein and NOS in neurons of cerebral cortex from fetal rats and whether Angelica sinensis has the protective effect on these neurons in hypoxia. DESIGN： Randomized control experiment.SETTING ： Department of Histology and Embryology, Luzhou Medical College.MATERIALS ： Twelve adult female Wistar rats in oestrum and 1 male Wistar rat with bodymass from 220 to 250 g were chosen. Parenteral solution of Angelica sinensis mainly contained angelica sinensis, 10 mL/ampoule, was provided by Department of Agent of the Second Hospital Affiliated to Hubei Medical University （batch number： 01062310）. METHODS ： This experiment was completed in the Department of Histology and Embryology of Luzhou Medical College from September 2003 to June 2004. ①Twelve adult female Wistar rats in oestrum and 1 male Wistar rat were housed in one rearing cage. Vaginal embolus was performed on conceive female rat at 8： 00 am next day. On the 15^th conceiving day, all conceiving rats were divided randomly into three groups： control group, hypoxia group and Angelica group with 4 in each group. Rats in hypoxia group and Angelica group were modeled with hypotonic hypoxia in uterus. Angelica group： Rats were injected with 8 mL/kg Angelica sinensis injection through caudal veins before hypoxia. Hypoxia group： Rats were injected with the same volume of saline. Control group： Rats were not modeled and fed with normal way. ② Twenty embryos of rats were chosen randomly from each group and then routinely embedded in paraffin. Paraffin sections were cut from the brain of embryos to anterior fontanelle. Double-label staining was used to detect the expression of nNOS and c-Fos in neurons of cerebral cortex from

关键词： Fos Expression of c-Fos protein and nitricoxide synthase in neurons of cerebral cortex from fetal rats in hypoxia and protective role of Angelica sinensis