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Pollen self-elimination CRISPR–Cas genome editing prevents transgenic pollen dispersal in maize

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Plant Communications 2023年第6期4卷 212-215页

作者： Honglin Wang Xiantao Qi Jinjie Zhu Changlin Liu hongwei fan Xuecai Zhang Xinhai Li Qin Yang Chuanxiao Xie Institute of Crop Science Chinese Academy of Agricultural Sciences(CAAS)Beijing 100081China College of Agronomy Northwest A&F UniversityYangling 712100China International Maize and Wheat Improvement Center(CIMMYT) EI BatanMexico Hainan Yazhou Bay Seed Lab SanyaHainan Province 572024 China Henan Jinyuan Seed Industry Co. Ltd.ZhengzhouHenan ProvinceChina

Dear Editor,Genome editing with clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated nuclease(Cas)-mediated technologies have revolutionized basic plant science and crop genetic improvement(Chen et al.,2019).Stable genetic transformation of CRISPR–Cas cassette(s)is the main approach to genome editing in planta.In many sexually reproducing plants,a major concern is the dispersal of genetically modified elements through pollen(Devos et al.,2005).Maize(Zea mays L.).

关键词： CRISPR dispersal editing

Determination of fenticonazole in human plasma by HPLC–MS/MS and its application to pharmacokinetic studies

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Journal of Pharmaceutical Analysis 2017年第1期7卷 63-70页

作者： Weixing Mao Yiya Wang Wenhui Hu Feifei Jiao hongwei fan Li Ding Department of Pharmaceutical Analysis China Pharmaceutical University Nanjing 210009 China Nanjing Clinical Tech. Laboratories Inc. 18 Zhilan Road Jiangning District Nanjing 211000 China Department of Clinical Pharmacology Nanjing First Hospital Nanjing Medical University Nanjing Jiangsu 210006 China

Two simple and sensitive high performance liquid chromatography–tandem mass spectrometry(HPLC–MS/MS) methods were developed and validated for the determination of fenticonazole in human plasma after percutaneous and intravaginal administration. Mifepristone was used as an internal standard(IS), and simple protein precipitation by acetonitrile containing 2% acetic acid was utilized for extracting the analytes from the plasma samples. Chromatographic separation was performed on a Kinetex XB-C_(18) column. The quantitation was performed by a mass spectrometer equipped with an electrospray ionization source in multiple reactions monitoring(MRM) positive ion mode using precursor-to-product ion transitions of m/z 455.2–199.1 for fenticonazole and m/z 430.2–372.3 for mifepristone. The validated linear ranges of fenticonazole were 5–1000 pg/m L and 0.1–20 ng/m L in plasma for the methods A and B, respectively. For the two methods, the accuracy data ranged from 85% to 115%, the intra- and inter-batch precision data were less than 15%, the recovery data were more than 90%, and no matrix interference was observed. The methods A and B were successfully validated and applied to the pharmacokinetic studies of fenticonazole gel in Chinese healthy volunteers after percutaneous and intravaginal administration, respectively.

关键词： Fenticonazole HPLC–MS/MS Pharmacokinetic studies Human plasma

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急性发热伴多浆膜腔积液1例

中华内科杂志 2024年第1期63卷 94-96页

作者：刘慧婷沈敏范洪伟曹玮中国医学科学院、北京协和医学院、北京协和医院感染内科北京100730 中国医学科学院、北京协和医学院、北京协和医院风湿免疫科北京100730

本文报道了一例以反复发热及多浆膜腔积液起病的青年女性的诊疗过程。该患者在病程中在未应用针对性治疗的情况下症状及炎症指标即呈现自发缓解及复发趋势,影像学也可见胸腔及心包积液在短时间内明显变化。在进行基础筛查除外常规感染... 详细信息

本文报道了一例以反复发热及多浆膜腔积液起病的青年女性的诊疗过程。该患者在病程中在未应用针对性治疗的情况下症状及炎症指标即呈现自发缓解及复发趋势,影像学也可见胸腔及心包积液在短时间内明显变化。在进行基础筛查除外常规感染、自身免疫性疾病及肿瘤性疾病后,经全外显子组基因检测后发现患者携带与自身炎症性疾病中的家族性地中海热相关的MEFV E148Q杂合突变,并在加用秋水仙碱治疗后,患者症状消失,未再发作。

关键词：家族性地中海热心包积液胸腔积液发热

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Neural Regeneration Research 2013年第25期8卷 2360-2369页

作者： Guanqun Qiao Qingquan Li Gang Peng Jun Ma hongwei fan Yingbin Li Department of Neurosurgery the Second Affiliated Hospital of Nanjing Medical University Department of Pharmacology the Third Affiliated Hospital of Nanjing Medical University

Although it is believed that glioma is derived from brain tumor stem cells, the source and molecular signal pathways of these cells are still unclear. In this study, we used stable doxycycline-inducible transgenic mouse brain tumor models （c-myc/SV40Tag＋/Tet-on＋） to explore the malignant trans- formation potential of neural stem cells by observing the differences of neural stem cells and brain tumor stem cells in the tumor models. Results showed that chromosome instability occurred in brain tumor stem cells. The numbers of cytolysosomes and autophagosomes in brain tumor stem cells and induced neural stem cells were lower and the proliferative activity was obviously stronger than that in normal neural stem cells. Normal neural stem cells could differentiate into glial fibrillary acidic protein-positive and microtubule associated protein-2-positive cells, which were also negative for nestin. However, glial fibrillary acidic protein/nestin, microtubule associated protein-2/nestin, and glial fibrillary acidic protein/microtubule associated protein-2 double-positive cells were found in induced neural stem cells and brain tumor stem cells. Results indicate that induced neural stem cells are similar to brain tumor stem cells, and are possibly the source of brain tumor stem cells.

关键词： neural regeneration stem cells neural stern cells brain tumor stem cells subventricular zone braintumor transgenic mouse model multidirectional differentiation doxycycline neuroregeneration

Acoustics Based Monitoring and Diagnostics for the Progressive Deterioration of Helical Gearboxes

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Chinese Journal of Mechanical Engineering 2021年第4期34卷 98-109页

作者： Kaibo Lu James Xi Gu hongwei fan Xiuquan Sun Bing Li Fengshou Gu College of Mechanical and Vehicle Engineering Taiyuan University of TechnologyTaiyuan 030024China School of Engineering University of BoltonBolton BL35ABUK College of Mechanical Engineering Xi’an University of Science and TechnologyXi’an 710054China Centre for Efficiency and Performance Engineering University of HuddersfieldHuddersfield HD13DHUK. School of Industrial Automation Beijing Institute of TechnologyZhuhai 519088China

Gearbox condition monitoring(CM)plays a significant role in ensuring the operational reliability and efficiency of a wide range of critical industrial systems such as wind turbines and helicopters.Accurate and timely diagnosis of gear faults will improve the maintenance of gearboxes operating under sub-optimal conditions,avoid excessive energy consumption and prevent avoidable damages to systems.This study focuses on developing CM for a multi-stage helical gearbox using airborne sound.Based on signal phase alignments,Modulation Signal Bispectrum(MSB)analysis allows random noise and interrupting events in sound signals to be suppressed greatly and obtains nonlinear modulation features in association with gear dynamics.MSB coherence is evaluated for selecting the reliable bi-spectral peaks for indication of gear deterioration.A run-to-failure test of two industrial gearboxes was tested under various loading conditions.Two omnidirectional microphones were fixed near the gearboxes to sense acoustic information during operation.It has been shown that compared against vibration based CM,acoustics can perceive the responses of vibration in a larger areas and contains more comprehensive and stable information related to gear dynamics variation due to wear.Also,the MSB magnitude peaks at the first three harmonic components of gear mesh and rotation components are demonstrated to be sufficient in characterizing the gradual deterioration of gear transmission.Consequently,the combining of MSB peaks with baseline normalization yields more accurate monitoring trends and diagnostics,allowing the gradual deterioration process and gear wear location to be represented more consistently.

关键词： Acoustics Modulation Signal Bispectrum Helical gearbox Gear wear

高通量rGO微球@PVDF纳米纤维复合膜的制备及其油水分离性能研究

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化学工业与工程 2024年第2期41卷 47-56页

作者：来晨雨王瑞程丝楠范红玮孟洪北京化工大学化学工程学院新疆大学化学学院

采用静电纺丝法制备了聚偏氟乙烯(PVDF)纳米纤维膜，并采用共混纺丝法和真空抽滤两种方式将还原氧化石墨烯微球(rGO)负载于其上，获得高通量rGO微球@PVDF纳米纤维复合油水分离膜。通过调整静电纺丝过程参数(如推注速率和电场强度等)和... 详细信息

采用静电纺丝法制备了聚偏氟乙烯(PVDF)纳米纤维膜，并采用共混纺丝法和真空抽滤两种方式将还原氧化石墨烯微球(rGO)负载于其上，获得高通量rGO微球@PVDF纳米纤维复合油水分离膜。通过调整静电纺丝过程参数(如推注速率和电场强度等)和纺丝液配方，对PVDF纳米纤维膜结构进行优化，并采用不同的rGO微球负载量、负载方式、黏结剂含量来提高纳米纤维膜的表面粗糙度和疏水性。利用扫描电子显微镜和接触角测试对纤维膜的表面形貌和亲疏水性进行表征，并通过二氯甲烷-水体系进行油水分离实验，测试了不同配方下杂化膜的重力驱动油水分离性能。结果表明，当静电纺丝溶液中PVDF含量为14%时，以1 mL·h-1的推注速率，在15 kV下制得的PVDF纳米纤维膜，并将1%PVDF溶液、3 mg rGO微球(与黏结剂中有效成分PVDF质量比为3∶1)和溶剂组成的铸膜液抽滤在膜表面，复合膜表面水接触角为130.9°,其油水分离过程中的有机溶剂透过通量可达5 641.3 L·h-1·m-2,水相的截留率为99.28%。

关键词：静电纺丝聚偏氟乙烯还原氧化石墨烯微球油水分离膜

一种基于暗亮通道分割融合的低照度环境图像去尘雾及增强方法

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煤炭学报 2024年第4期49卷 2167-2178页

作者：樊红卫张超曹现刚刘金鹏张旭辉赵寒西安科技大学机械工程学院陕西西安710054 西安科技大学陕西省矿山机电装备智能检测与控制重点实验室陕西西安710054 陕西彬长胡家河矿业有限公司陕西咸阳713600

受煤矿井下粉尘、水雾和低照度环境影响,对皮带运输系统的监测图像精准识别极为困难。针对现有去尘雾方法的图像处理结果和效率欠佳的问题,提出一种基于暗亮通道分割融合的低照度环境图像去尘雾及增强方法。首先利用阈值分割结合伽马变... 详细信息

受煤矿井下粉尘、水雾和低照度环境影响,对皮带运输系统的监测图像精准识别极为困难。针对现有去尘雾方法的图像处理结果和效率欠佳的问题,提出一种基于暗亮通道分割融合的低照度环境图像去尘雾及增强方法。首先利用阈值分割结合伽马变换修正通道差,解决因低照度环境影响导致的尘雾浓度较大区域与其他区域间像素值差异不明显的问题,修正后通过引导尘雾图像做引导滤波得到更加符合实际情况的全局大气光强;然后为解决暗通道先验在尘雾浓度较大区域失效问题,引入亮通道先验进行补充,使用通道分量来辅助暗通道及亮通道透射率融合,避免因多次分割而导致的边缘像素归属问题;最后将去雾后RGB图像转至HSV空间,对亮度分量进行直方图均衡化并将均衡化前后的亮度分量进行加权融合,采用客观指标评价,选择最优聚合权值进行聚合,同时考虑去雾过程中饱和度损失和亮度分量与饱和度分量间的相关性提出饱和度自适应矫正函数,对图像饱和度进行矫正,色调分量保持不变,随后将图像转回至RGB空间,得到亮度适中、信息保留丰富和色彩鲜艳的图像;为验证所提方法的有效性,采用主观视觉、客观指标和目标检测精度及置信度进行算法对比,实验结果表明所提方法在上述4个指标上均优于被对比算法,其图像细节保留丰富,图像视觉观感更佳。

关键词：低照度暗通道亮通道分割融合图像去雾图像增强

南京市DRG改革前后脑血管病介入治疗住院费用分析

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中国医药导报 2024年第2期21卷 70-75页

作者：李佳艺顾民樊宏伟南京医科大学公共卫生学院江苏南京211100 南京医科大学第二附属医院泌尿外科江苏南京210000 南京市第一医院药学部江苏南京210006

目的以脑血管介入治疗(BE29)病组为例探索南京定点医疗机构2022年起实施的疾病诊断相关分组(DRG)支付改革效果,为该病种的细化规则提供参考。方法回顾性收集南京某两家公立医院神经外科2021年(改革前,349例)、2022年(改革后,427例)BE29... 详细信息

目的以脑血管介入治疗(BE29)病组为例探索南京定点医疗机构2022年起实施的疾病诊断相关分组(DRG)支付改革效果,为该病种的细化规则提供参考。方法回顾性收集南京某两家公立医院神经外科2021年(改革前,349例)、2022年(改革后,427例)BE29病组的出院患者,分析改革前后该病组患者人均费用及其结构变化,评估DRG控费效果。根据2022年住院总费用倍率分析DRG改革后盈亏情况。将BE29病组按诊断类型(脑梗死、不伴有梗死的颅内血管阻塞和狭窄、颅内动脉瘤)进一步分组,分析各亚组病例费用的差异及亚组划分的可行性,并对费用最高的亚组进行费用的影响因素探讨。结果改革后,BE29病组人均药品费用低于改革前,人均耗材和人均检查费用高于改革前,差异有统计学意义(P<0.05)。住院天数与倍率呈正相关,各项费用随着倍率的升高而增加,差异有统计学意义(P<0.05)。改革前后,动脉瘤亚组费用均高于两个脑缺血亚组,差异有统计学意义(P<0.05);在脑缺血患者中,改革后脑梗死亚组费用高于未伴有梗死的颅内血管阻塞或狭窄亚组,差异有统计学意义(P<0.05);改革前后,BE29总病组医疗费用高于亚组脑梗死(BE29.1)及颅内血管阻塞和狭窄介入治疗(BE29.2)的费用,低于颅内动脉瘤介入治疗(BE29.3)的费用,差异有统计学意义(P<0.05)。不同年龄、动脉瘤的破裂、多发性、支架辅助栓塞及住院时间与颅内动脉瘤介入治疗住院费用比较,差异有统计学意义(P<0.05)。在多因素分析中,动脉瘤的多发性和住院天数是费用的独立影响因素(P<0.05)。结论BE29病组应综合考虑不同诊断类型费用及其影响因素的差异性,细化付费规则的制订,更体现医疗劳务价值。

关键词：脑血管介入治疗疾病诊断分组 DRG支付改革南京

基于EMDPWVD时频图像和改进ViT网络的滚动轴承智能故障诊断

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振动与冲击 2024年第11期43卷 246-254页

作者：樊红卫马宁阁马嘉腾陈步冉曹现刚张旭辉西安科技大学机械工程学院西安710054 陕西省矿山机电装备智能检测与控制重点实验室西安710054

滚动轴承是机械设备的关键零部件之一,其故障诊断对设备安全稳定运行至关重要。针对滚动轴承振动信号的非平稳特点,提出经验模态分解联合伪魏格纳分布(empirical mode decomposition&pseudo-Wigner-Ville distribution, EMDPWVD)时频图... 详细信息

滚动轴承是机械设备的关键零部件之一,其故障诊断对设备安全稳定运行至关重要。针对滚动轴承振动信号的非平稳特点,提出经验模态分解联合伪魏格纳分布(empirical mode decomposition&pseudo-Wigner-Ville distribution, EMDPWVD)时频图像联合改进Vision Transformer(ViT)网络模型的智能故障诊断新方法。首先针对实际信号研究短时傅里叶变换(short-time Fourier transform, STFT)、连续小波变换(continuous wavelet transform, CWT)和EMDPWVD三种时频分析方法,考虑STFT和CWT无法同时获得高的时间分辨率和频率分辨率,优选EMDPWVD作为智能故障诊断网络的时频图像构造方法。其次,以经典ViT作为故障诊断基础模型,将时频图像按照预定尺寸分块并线性映射为输入序列,通过自注意力机制整合图像全局信息,借助堆叠Transformer编码器完成网络传输,进而实现故障诊断。为进一步提高故障诊断准确率,将池化层作为ViT的预处理网络,获得改进的Pooling ViT(PiT)模型,实现时频图像的空间特征延展,提升模型对输入图像敏感度。结果表明,所提方法对滚动轴承不同故障类型均有高的诊断准确率,PiT较ViT的准确率提高4.40%,证明对ViT加入池化层能够实现滚动轴承故障诊断效果提升。

关键词：滚动轴承故障诊断时频图像 Vision Transformer(ViT) 池化层