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Large-Scale CRISPR Screen of LDLR Pathogenic Variants

Research 2024年第2期2023卷 213-225页

作者： Mengjing Li Lerong Ma Yiwu Chen Jianing Li Yanbing Wang Wenni You hongming yuan Xiaochun Tang Hongsheng Ouyang Daxin Pang Key Lab for Zoonoses Research Ministry of EducationAnimal Genome Editing Technology Innovation CenterCollege of Animal SciencesJilin UniversityChangchunJilin Province 130062China The Institute of Translational Medicine Tianjin Union Medical Center of Nankai UniversityTianjin 300071China Chongqing ResearchInstitute Jilin UniversityChongqing 401123China Chongqing Jitang Biotechnology Research Institute Co.Ltd. ChongqingChina

Familial hypercholesterolemia(FH)is a frequently occurring genetic disorder that is linked to early-onset cardiovascular *** left untreated,patients with this condition can develop severe cardiovascular ***,many patients remain undiagnosed,and even when diagnosed,the treatment is often not *** mutations in the LDLR gene are the primary cause of FH,predicting whether novel variants are pathogenic is not a straightforward *** the functionality of LDLR variants is crucial in uncovering the genetic basis of *** study utilized CRISPR/Cas9 cytosine base editors in pooled screens to establish a novel approach for functionally assessing tens of thousands of LDLR variants on a large scale.A total of more than 100 single guide RNAs(sgRNAs)targeting LDLR pathogenic mutations were successfully screened with relatively high *** of these,5 sgRNAs were further subjected to functional verification studies,including 1 in the promoter,1 in the antisense RNA,1 in the exon,and 2 in the *** for the variant caused by the sgRNA located at intron 16,the functionalities of the other LDLR variants were all *** high similarity of LDLR intron sequences may lead to some false ***,these results confirm the reliability of the large-scale screening strategy for functional analysis of LDLR variants,and the screened candidate pathogenic mutations could be used as an auxiliary means of clinical gene detection to prevent FH-induced heart disease.

关键词： LDLR Screen straight

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Cagelike mesoporous silica encapsulated with microcapsules for immobilized laccase and 2,4-DCP degradation

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Journal of Environmental Sciences 2015年第12期27卷 52-62页

作者： Junya Yang Yan Huang Yuxiang Yang hongming yuan Xiangnong Liu School of Chemistry and Molecular Engineering East China University of Science & Technology State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of ChemistryJilin University Analysis Test Center Yangzhou University

In this study,cage-like mesoporous silica was used as the carrier to immobilize laccase by a physical approach,followed by encapsulating with chitosan/alginate microcapsule membranes to form microcapsules of immobilized laccase based on layer-by-layer *** relationship between laccase activity recovery/leakage rate and the coating thickness was simultaneously *** the microcapsule layers have a substantial network of pores,they act as semipermeable membranes,while the laccase immobilized inside the microcapsules acts as a processing plant for degradation of2,*** microcapsules of immobilized laccase were able to degrade 2,4-dichlorophenol within a wide range of 2,4-dichlorophenol concentration,temperature and p H,with mean degradation rate around 62%.Under the optimal conditions,the thermal stability and reusability of immobilized laccase were shown to be improved significantly,as the removal rate and degradation rate remained over 40.2% and 33.8% respectively after 6 cycles of *** mass spectrometry（MS） and nuclear magnetic resonance（NMR）,diisobutyl phthalate and dibutyl phthalate were identified as the products of 2,4-dichlorophenol degradation by the microcapsules of immobilized laccase and laccase immobilized by a physical approach,respectively,further demonstrating the degradation mechanism of 2,4-dichlorophenol by microcapsule-immobilized laccase.

关键词： Laccase-cMSS mLbL-laccase 2,4-Dichlorophenol Degradation efficiency Reusability Degradation mechanism

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Late Quaternary Strata and Carbon Burial Records in the Yellow River Delta, China

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Journal of Ocean University of China 2015年第3期14卷 446-456页

作者： ZHAO Guangming YE Siyuan LI Guangxue DING Xigui yuan hongming College of Marine Geo-science Ocean University of China The Key Laboratory of Coastal Wetlands Biogeosciences China Geologic Survey The Key Laboratory of Marine Hydrocarbon Resource and Geology Ministry of Land and Resources

Sediment carbon sequestration plays an essential role in mitigating atmospheric CO2 increases and the subsequently global greenhouse effect. To clarify the late Quaternary strata and carbon burial records in YeUow River delta （YRD）, detailed analysis of benthic foraminifera, total carbon （TC）, organic carbon （Corg）, sedimentary characteristics and moisture contents of sediments, was performed on core ZK3, 30.3 m in length and obtained from YRD in 2007. Eight depositional units （designated U1-U8 in ascending order） were identified. A comprehensive analysis method of historical geography and sedimentary geology was used to de- termine the precise depositional ages of the modem Yellow River delta （MYRD）, from which pre-MYRD ages were deduced. The results indicates that the maximum burial rates of TC, inorganic carbon （IC） and Corg occurred in the delta front （U5）, and the mini- mum in the shallow sea （U3）. Remarkable high sedimentation rates in the MYRD are responsible for burial efficiency of carbon, with an average rate of Corg burial reaching 2087±251 g（m2yr）-1, and that of IC reaching 13741±808g（m2yr）-1, which are much higher than those of other regions with high contents of Corg. Therefore, YRD has a significant burial efficiency for carbon sequestration.

关键词： Yellow River delta late Quaternary strata carbon burial rate organic carbon inorganic carbon

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Degradation of 2,4-DCP by the Immobilized Laccase on the Carrier of Fe304@SiO2-NH2

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Chinese Journal of Chemistry 2012年第12期30卷 2849-2860页

作者： Huan Weiwei Yang Yuxiang Wu Bin yuan hongming Zhang Yani Liu Xiangnong School of Chemistry and Molecular Engineering East China University of Science & Technology Shanghai 200237 China State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of Chemistry Jilin University Changchun Jilin 130012 China Analysis Test Center Yangzhou University Yangzhou Jiangsu 225009 China

In this paper, the laccase immobilized on Fe304@SiO2-NH2 nanoparticles was successfully prepared by the glutaraldehyde cross-linking method. The degradations of 2,4-diehlorophenol （2,4-DCP） catalyzed by laccase and immobilized laccase were carried out. The optimal conditions regarding degradation efficiency were also discussed, which include reaction time, pH value, temperature, concentration of 2,4-DCP and laccase. When laccase was immobilized on Fe304@SiO2-NH2 carrier by crosslinking with glutaraldehyde, the stability and repetition were im- proved significantly. The removal efficiency of 2,4-DCP by immobilized laccase still remained over 59% after six cycles of operation. Degradation of 2,4-DCP is a first-order reaction and the activation energies of 2,4-DCP catalyzed by laccase and immobilized laccase are 51.93 kJ·mol-1 strate the immobilized laccase had a faster degradation Fe304@MSS-NH2 can promote the degradation reaction. and 44.12 kJ·mol-1, respectively. The results demonrate than the free laccase; the magnetic carrier

关键词： Fe3O4@SiO2-NH2 nanoparticles immobilized laccase degradation rate repetition magneto-inducedeffect

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Hydrothermal Synthesized Co-Ni3S2 Ultrathin Nanosheets for Efficient and Enhanced Overall Water Splitting

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Chemical Research in Chinese Universities 2019年第2期35卷 179-185页

作者： JIAN Juan yuan Long LI He LIU Huanhuan ZHANG Xinghui SUN Xuejiao yuan hongming FENG Shouhua State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of ChemistryJilin UniversityChangchun 130012P.R.China Key Laboratory of Functional Materials Physics and Chemistry Ministry of EducationJilin Normal UniversitySiping 136000R R. China

We used the one-step hydrothennal controlled synthesis method for Co-Ni3S2 ultrathin nanosheets grown directly on nickel foam(NF).The as-synthesized Co-Ni3S2/NF showed eiilianced activities in the hydrogen evolution reaction(HER),oxygen evolution reaction(OER)and better overall water splitting(OWS)efficiency than the iin-doped Ni3S2/*** voltage of Co-Ni3S2/NF for OWS was only 1.58 V at the current density of 10 niA/cm^2 and with long time(>30 h)current output during the current-density(i-t)*** good i-t pertonnance was also observed in both HER and OER ***,the Co-Ni3S2/NF showed a large current density(>1A/cm^2)for both HER and *** the current densities reached 100 and 1000 mA/cm^2,the required overpotentials tor Co-Ni3S2/NF were 0.35 and 0.75 V for OER and 0.30 and 0.85 V for ***,after introducing Co,the activity of Ni3S2-based material was strongly enhanced.

关键词： Co-Ni3S2/nickel tbam(NF) Ultrathin nanosheet Water splitting Hydrogen evolution reaction Oxygen evolution reaction

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Degradation of 2,4-dichlorophenol catalyzed by the immobilized laccase with the carrier of Fe_3O_4@MSS–NH_2

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Chinese Science Bulletin 2014年第5期59卷 509-520页

作者： Yan Huang Yanjie Xi Yuxiang Yang Cheng Chen hongming yuan Xiangnong Liu School of Chemistry and Molecular Engineering East China University of Science & Technology State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of Chemistry Jilin University Analysis Test Center Yangzhou University

With the Fe3O4@MSS(magnetic mesoporous silica spheres)–NH2as the carrier and the glutaraldehyde as crosslinking agent,the immobilized laccase has been prepared and characterized by XRD,IR,SEM and BET *** the optimal conditions,the removal efficiency of2,4-DCP reached 88%and the removal efficiency still remained 61.5%after five cycles of *** virtue of GC–MS analysis,2,5-dimethoxyl-1,4-quinone,2-chlorine-1,4-dimethoxyl benzene,3,30-dichlorine-4,40-dimethoxyl biphenyl,maleic acid phenol ester,and three kinds of maleic acid,alcohol ester,and para-hydroxyl phenol ester compounds have been identified as intermediate and final degradation products of 2,4-DCP,***,the degradation products of 2,4-DCP have been confirmed by performing the1H-NMR and13C-NMR experiments,further demonstrating the degradation mechanism of 2,4-DCP by the immobilized laccase.

关键词：二氯苯酚固定化酶降解 GC-MS分析 13C-NMR 载波催化 1H-NMR

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Multi-process production occurs in the iron and steel industry,supporting‘dual carbon'target:An in-depth study of CO_(2)emissions from different processes

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Journal of Environmental Sciences 2024年第6期140卷 46-58页

作者： hongming Na Yuxing yuan Tao Du Tianbao Zhang Xi Zhao Jingchao Sun Ziyang Qiu Lei Zhang SEP Key Laboratory of Eco-Industry Northeastern UniversityShenyang 110819China School of Metallurgy Northeastern UniversityShenyang 110819China

Reducing CO_(2)emissions of the iron and steel industry,a typical heavy CO_(2)-emitting sector is the only way that must be passed to achieve the‘dual-carbon’goal,especially in *** previous studies,however,it is still unknown what is the difference between blast furnace basic oxygen furnace(BF-BOF),scrap-electric furnace(scrap-EF)and hydrogen metallurgy *** quantitative research on the key factors affecting CO_(2)emissions is insufficient There is also a lack of research on the prediction of CO_(2)emissions by adjusting industria *** on material flow analysis,this study establishes carbon flow diagrams o three processes,and then analyze the key factors affecting CO_(2)***_(2)emissions of the iron and steel industry in the future is predicted by adjusting industrial structure The results show that:(1)The CO_(2)emissions of BF-BOF,scrap-EF and hydrogen metallurgy process in a site are 1417.26,542.93 and 1166.52 kg,respectively.(2)By increasing pellet ratio in blast furnace,scrap ratio in electric furnace,etc.,can effectively reduce CO_(2)emissions(3)Reducing the crude steel output is the most effective CO_(2)reduction *** is still 5.15×10^(8)-6.17×10^(8) tons of CO_(2)that needs to be reduced by additional measures.

关键词： Blast furnace-basic oxygen furnace process Scrap-electric furnace process Hydrogen metallurgy process Carbon flow diagram Influencing factors CO_(2)emission prediction

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Hydrothermal synthesis and characterization of rare-earth ruthenate pyrochlore compounds R_2Ru_2O_7(R=Pr_(3+),Sm(3+)-Ho(3+))

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Science China Chemistry 2011年第6期54卷 941-946页

作者： YAO LinRan, WANG Dong, PENG Wen, HU WeiWei, yuan hongming & FENG ShouHua State key Laboratory of Inorganic Synthesis and Preparative Chemistry College of Chemistry, Jilin University, Changchun 130023, China State key Laboratory of Inorganic Synthesis and Preparative Chemistry College of Chemistry Jilin University Changchun China

A series of pyrochlore oxides, R2Ru2O7 (R=Pr3+, Sm3+–Ho3+) were synthesized under mild hydrothermal conditions. All the samples crystallize in uniform octahedron characteristically. The products were characterized by powder X-ray diffraction, scanning electron microscopy, energy-disperse X-ray spectroscopy, and dc susceptibility, and the factors that affected the crystallization were discussed. It was found that the purity of products depends strongly on the raw materials and the amount of alkalinity in the initial reaction mixtures. The ZFC and FC susceptibilities of all of the compounds R2Ru2O7 at low temperature were also measured and discussed.

关键词： hydrothermal synthesis pyrochlore compounds magnetic properties

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Prompt learning in computer vision: a survey

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Frontiers of Information Technology & Electronic Engineering 2024年第1期25卷 42-63页

作者： Yiming LEI Jingqi LI Zilong LI yuan CAO hongming SHAN Shanghai Key Laboratory of Intelligent Information Processing School of Computer ScienceFudan UniversityShanghai 200438China Institute of Science and Technology for Brain-Inspired Intelligence Fudan UniversityShanghai 200433China MOE Frontiers Center for Brain Science Fudan UniversityShanghai 200433China Shanghai Center for Brain Science and Brain-Inspired Technology Shanghai 201210China

Prompt learning has attracted broad attention in computer vision since the large pre-trained visionlanguagemodels (VLMs) exploded. Based on the close relationship between vision and language information builtby VLM, prompt learning becomes a crucial technique in many important applications such as artificial intelligencegenerated content (AIGC). In this survey, we provide a progressive and comprehensive review of visual promptlearning as related to AIGC. We begin by introducing VLM, the foundation of visual prompt learning. Then, wereview the vision prompt learning methods and prompt-guided generative models, and discuss how to improve theefficiency of adapting AIGC models to specific downstream tasks. Finally, we provide some promising researchdirections concerning prompt learning.

关键词： Prompt learning Visual prompt tuning(VPT) Image generation Image classification Artificial intelligence generated content(AIGC)

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黄河三角洲利津超级叶瓣时空范围的再认识

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地理学报 2019年第1期74卷 146-161页

作者：何磊叶思源袁红明薛春汀中国地质调查局滨海湿地生物地质重点实验室青岛266071 青岛海洋科学与技术国家实验室海洋矿产资源评价与探测技术功能实验室青岛266061 中国地质调查局青岛海洋地质研究所青岛266071

黄河三角洲是世界著名的河流三角洲之一。自有学者首次系统地提出黄河三角洲全新世10期超级叶瓣的时空分布范围后,这一观点近30年来未见明显的改进。借助渤海湾南岸研究区获得的4个20～30 m钻孔地层资料和相应的AMS14C测年数据,以及前... 详细信息

黄河三角洲是世界著名的河流三角洲之一。自有学者首次系统地提出黄河三角洲全新世10期超级叶瓣的时空分布范围后,这一观点近30年来未见明显的改进。借助渤海湾南岸研究区获得的4个20～30 m钻孔地层资料和相应的AMS14C测年数据,以及前人部分钻孔的研究成果,本文对黄河三角洲利津超级叶瓣的沉积演化框架和时空分布特征进行了重新厘定:研究区全新世沉积环境由早期的河道和泛滥平原沉积,逐渐向海侵过程中盐沼、潮坪和浅海相演化。海平面上升速率减缓之后三角洲开始发育并逐步成陆,直至最后被陆相沉积所覆盖。黄河下游古河道在9000-8000 cal a BP左右可能在黄骅南部入海;大约8000 cal a BP之后,黄河主河道南迁到滨州—惠民一带,并在7000-5500 cal a BP之间发育利津超级叶瓣;利津超级叶瓣的北界可能在马山子和阳信一界,而南界在郭井子和弥河之间;约5500 cal a BP之后,黄河下游古河道可能重新过黄骅入海,于5500-3500 cal a BP左右形成黄骅超级叶瓣;约3500 cal a BP之后,黄河古河道北迁至天津,直至～700 BC左右主流河道入海口再次南迁至河北岐口附近。

关键词：黄河三角洲超级叶瓣河道变迁贝壳堤沉积演化

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