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Cardiac autonomic nerve fiber regeneration in chronic heart failure Do Akt gene-transduced mesenchymal stem cells promote repair?

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Neural Regeneration Research 2010年第1期5卷 28-34页

作者： hongliang kong Zhanquan Li Shumei Zhao Li Zhu Yingjun Zhao Weiwei Zhang GuipingXu Wenjun Hao Huijun Li Guoxian Qi Department of Cardiology the First Affiliated Hospital of China Medical University Shenyang 110001 Liaoning Province China Department of Cardiology the People's Hospital of Liaoning Province Shenyang 110016 Liaoning Province China Shenyang Normal University Shenyang 110034 Liaoning Province China

BACKGROUND： Transplantation of Akt-over-expressing mesenchymal stem ceils （Akt-MSCs） has been shown to repair infarcted myocardium and improve cardiac function. However, little is known about the therapeutic effects of Akt-MSCs on cardiac autonomic neuropathy in chronic heart failure （CHF）. OBJECTIVE： The present study used adriamycin-induced CHF rat models to observe the effect of Akt-MSCs on cardiac autonomic nervous regeneration and the factors mediating this effect. DESIGN, TIME AND SETTING： A randomized, controlled animal experiment was performed at the Central Laboratory of Basic Medical College, China Medical University, between September 2008 and April 2009. MATERIALS： Rabbit anti-choline acetyltransferase （CHAT）, growth associated protein-43 （GAP-43） synaptophysin （SYN） polyclonal antibodies and the secondary antibody （goat anti-rabbit IgG） were purchased from Boster, China. Cat-A-Kit assay system was provided by Amersham, USA. METHODS：（1） Adult rat MSCs were isolated and cultured for the preparation of Akt-MSCs. （2） Forty male Wistar rats were intramyocardially administered adriamycin at 2 mg/kg over 3 days for a total of five times and once a week for additional five times thereafter to establish CHF models. At 2 weeks after final adriamycin treatment, 34 successful CHF rat models were randomized to three groups： Akt-MSCs （n = 11）, simple MSCs （s-MSCs, n =11）, and control （n = 12）. Each group was intravenously administered Akt-MSCs （2x106 cells in 100 IJL PBS）, s-MSCs （2×10^6 cells in 100 μL PBS） or equal volume of phosphate buffered saline, once a day for a total of three times. MAIN OUTCOME MEASURES： At 4 weeks after final adriamycin treatment, myocardial norepinephrine （NE） content was detected using a Cat-A-Kit assay system. Myocardial CHAT, SYN and GAP-43 were performed by immunohistochemistry and Western blot analysis. Prior to, 2 and 4 weeks after adriamycin treatment, echocardiographic examination was performed and left ventricular ejection fract

关键词： mesenchymal stem cells Akt gene transfection chronic heart failure neural regeneration autonomic nerve system

Association of accelerometer-measured sleep duration and different intensities of physical activity with incident type 2 diabetes in a population-based cohort study

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Journal of Sport and Health Science 2024年第2期13卷 222-232页

作者： Xinyi Jin Yilin Chen hongliang Feng Mingqing Zhou Joey W.Y.Chang Yaping Liu Alice Pik Shan kong Xiao Tan Yun-Kwok Wing Yannis Yan Liang Jihui Zhang Guangdong Cardiovascular Institute Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou 510080China Guangdong Provincial Key Laboratory of Coronary Heart Disease Prevention Guangdong Cardiovascular InstituteGuangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhou 510080China School of Medicine South China University of TechnologyGuangzhou 510641China Center for Sleep and Circadian Medicine The Affiliated Brain Hospital of Guangzhou Medical UniversityGuangzhou 510180China The Second School of Clinical Medicine Southern Medical UniversityGuangzhou 510282China Guangdong Mental Health Center Guangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhou 510120China Li Chiu kong Family Sleep Assessment Unit Department of PsychiatryFaculty of MedicineThe Chinese University of Hong KongHong Kong 999077China Department of Psychiatry Faculty of MedicineThe Chinese University of Hong KongHong Kong 999077China Department of Medicine and Therapeutics Faculty of MedicineThe Chinese University of Hong KongHong Kong 999077China Department of Medical Sciences Uppsala UniversityUppsala 75185Sweden Department of Big Data in Health Science Zhejiang University School of Public HealthHangzhou 310058China Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China Guangzhou 510260China

Purpose:The aim of the current study was to investigate the association of accelerometer-measured sleep duration and different intensities of physical activity(PA)with the risk of incident type 2 diabetes in a population-based prospective cohort ***:Altogether,88,000 participants(mean age=62.2±7.9 years,mean±SD)were included from the UK *** duration(short:8 h/day)and PA of different intensities were measured using a wrist-won accelerometer over a 7-day period between 2013 and *** was classified according to the median or World Health Organization-recommendation:total volume of PA(high,low),moderate-to-vigorous PA(MVPA)(recommended,not recommended),and light-intensity PA(high,low).Incidence of type 2diabetes was ascertained using hospital records or death ***:During a median follow-up of 7.0 years,1615 incident type 2 diabetes cases were *** with normal sleep duration,short(hazard ratio(HR)=1.21,95%confidence interval(95%CI):1.03-1.41)but not long sleep duration(HR=1.01,95%CI:0.89-1.15)was associated with excessive type 2 diabetes *** increased risk among short sleepers seems to be protected against by *** with normal sleepers with high or recommended PA,short sleepers with low volume of PA(HR=1.81,95%CI:1.46-2.25),not recommended(below the World Health Organization-recommended level of)MVPA(HR=1.92,95%CI:1.55-2.36),or low light-intensity PA(HR=1.49,95%CI:1.13-1.90)had a higher risk of type 2 diabetes,while short sleepers with a high volume of PA(HR=1.14,95%CI:0.88-1.49),recommended MVPA(HR=1.02,95%CI:0.71-1.48),or high light-intensity PA(HR=1.14,95%CI:0.92-1.41)did ***:Accelerometer-measured short but not long sleep duration was associated with a higher risk of incident type 2 diabetes.A higher level of PA,regardless of intensity,potentially ameliorates this excessive risk.

关键词： Light physical activity Sleep duration Triaxial accelerometer Type 2 diabetes UK Biobank

Cell multiplication, apoptosis and p-Akt protein expression of bone mesenchymal stem cells of rat under hypoxia environment

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Journal of Nanjing Medical University 2007年第4期21卷 233-239页

作者： hongliang kong Ningning Liu Xin Huo Bo Wang Haipeng Zhang Mingyu Gao Guoxian Qi Department of cardiology the first affiliated hospital China Medical University Shenyang 110001 Liaoning Province China Department of Ophthalmology the first affiliated hospital China Medical University Shenyang 110001 China Department of general surgery the first affiliated hospital China Medical University S.henyang 110001 China Department of Pathophysiology China Medical University Shenyang 110001 China

Objective ：To elucidate whether cell multiplication, apoptosis, glucose intake and p-Akt protein expression of bone Mesenchyreal Stem Cells（MSCs） of rats is influenced by a hypoxic environment ex vivo. Methods ：Passage 3 of bone marrow MSCs taken from Wistar rats,were cultured in a culturing chamber with 94%N2,1%O2,5%CO2 at 37℃. At different hypoxia time points ,0,0.5, 1,4 and 8 h, glucose uptake was assayed by using radiation isotope ^3H-G, Apoptotic Rate（AR） and dead rate（DR） were analyzed by flow cytometry（FCM） after Annexin V/PI staining, cell multiplication（by MTr methods） and p-Akt protein by immunocytochemistry and western blot. Results ：Assay for CD29^± ,CD44^± ,CD71^± ,CD34^-, Tn T^±（after 5-azacytidine agent inducing） and ALP^±（after bone differentiation agent inducing） suggested these bone-derived cells were MSCs. The ^3H-G intaking ratio （CPM/ flask value：157 ± 11,110 ± 11,107 ± 13,103 ± 10,100 ± 9 and 98 ± 10） of MSCs at different hypoxia time points, significantly decreased compared to that of normoxia（P 〈 0.01） and tended to descend slowly with hypoxia time duration, for which there was no statistical significance（P 〉 0.05）. The AR（0.09 ± 2.03%,12.9 ± 1.72%,13.7 ± 2.26%,13.8 ± 3.01%,14.1 ± 2.78% and 14.7 ± 4.01% at 0,0.5,1,4 and 8 h,respectively,P 〈 0.01） and DR （0.04, ± 1.79% ,0.93 ± 1.85% ,3.11 ± 2.14% ,4.09 ± 2.36% ,4.72 ± 2.05% and 4.91 ± 3.72% at 0,0.5,1,4 and 8 h, respectively, P 〈 0.05） at different hypoxia time points significantly increased compared to those time in normoxia; The AR further went up with time （P 〈 0.05）, however there was no statistical significance （P 〉 0.05） for the DR. Optical absorption value of MTr methods at different hypoxia time points significantly decreased compared to those with a corresponding normoxia time （P 〈 0.01） and degraded with time （in an hypoxic environment -P 〈 0.01）. IOD of p-Akt protein of MSCs at different hypoxia time points significantly increased （0.367 ± 0.031,0.556 ± 0.023,0.579 ± 0.013, 0.660 ±

关键词： bone marrow mesenchymal stem cells of rat hypoxia glucose uptaking apoptotic ratio p-Akt protein

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永磁同步电动机无传感器矢量控制方案的研究

电气技术 2010年第4期11卷 15-19,24页

作者：孔洪亮白连平北京信息科技大学自动化学院北京100192

在永磁同步电动机无传感器控制中,通常通过测量进线电压和电流来估计转子磁链,并把估计的转子磁链作为无传感器矢量控制的反馈,从而达到使用传感器控制时的高性能。高速控制时,最关键的是保证控制过程中参数变量的鲁棒性。本文分析了基... 详细信息

在永磁同步电动机无传感器控制中,通常通过测量进线电压和电流来估计转子磁链,并把估计的转子磁链作为无传感器矢量控制的反馈,从而达到使用传感器控制时的高性能。高速控制时,最关键的是保证控制过程中参数变量的鲁棒性。本文分析了基于电压模型的古典开环转子磁链估计和较复杂的Luenberger转子磁链观测器在永磁同步电动机转子磁链估计上的差异,并使用STM32微处理器先后通过仿真和实验做了分析和论证。

关键词：无传感器矢量控制转子磁链观测器离散时域

人参皂甙Rbl对心力衰竭大鼠蛋白激酶R样内质网激酶途径的影响

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中国组织化学与细胞化学杂志 2013年第4期22卷 290-295页

作者：孔宏亮侯爱洁郭翠艳王俊丰辽宁省人民医院心脏中心沈阳110016

目的探讨人参皂甙Rbl(Gs-Rb1)改善阿霉素所致心力衰竭(HF)效应是否与调整蛋白激酶R样内质网激酶(PERK)通路有关。方法阿霉素(Adr)诱导的HF大鼠随机分为HF组(n=15)和Gs-Rbl组(70mg/kg/d,n=17),另随机选取同龄大鼠作为对照组(n=10)。干预... 详细信息

目的探讨人参皂甙Rbl(Gs-Rb1)改善阿霉素所致心力衰竭(HF)效应是否与调整蛋白激酶R样内质网激酶(PERK)通路有关。方法阿霉素(Adr)诱导的HF大鼠随机分为HF组(n=15)和Gs-Rbl组(70mg/kg/d,n=17),另随机选取同龄大鼠作为对照组(n=10)。干预结束并进行心脏超声检查后,TUNNEL检测心肌细胞凋亡率(AR),Western blot和Rt-PCR检测葡萄糖调节蛋白78(GRP78)、PERK、p-PERK、真核细胞起始因子2α(eIF2α)、p-eIF2α、C/EBP同源蛋白(CHOP)和cleaved caspase-12。结果 ***干预成功构建HF模型,Gs-Rb1显著提高左室射血分数(LVEF)和降低心肌细胞AR(P<0.01);***组GRP78mRNA和蛋白均显著高于对照组,Gs-Rb1显著低于HF组和对照组二组的表达(P<0.01);***-Rb1显著降低HF大鼠PERK和p-PERK表达(P<0.01);***导致eIF2αmRNA和蛋白、p-eIF2α显著升高,Gs-Rb1显著下调三者的表达(P<0.01);***组CHOP mRNA和蛋白显著高于对照组,Gs-Rb1组显著抑制其表达(P<0.01);***-Rb1显著抑制阿霉素所致的caspase-12mRNA和cleaved caspase-12蛋白表达(P<0.01)。结论 Gs-Rb1通过调节PERK内质网通路介导其改善HF效应。

关键词：人参皂甙Rb1 心力衰竭蛋白激酶R样内质网激酶真核细胞起始因子2α C/EBP同源蛋白

辛伐他汀对慢性心力衰竭大鼠血清白细胞介素18水平的影响

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郑州大学学报（医学版） 2009年第1期44卷 129-132页

作者：周书春杨树涵赵玉兰孔宏亮郑州大学第二附属医院心内科郑州450014 南阳医学高等专科学校附属医院心内科南阳473058

目的:观察慢性心力衰竭大鼠白细胞介素-18(IL-18)的变化以及辛伐他汀对其的影响。方法:Wistar大鼠75只,取假手术组(Sham组)10只,只分离腹主动脉不结扎作为对照,余大鼠采用肾上腹主动脉缩窄法制作慢性心力衰竭(心衰)模型。8周后入选心衰... 详细信息

目的:观察慢性心力衰竭大鼠白细胞介素-18(IL-18)的变化以及辛伐他汀对其的影响。方法:Wistar大鼠75只,取假手术组(Sham组)10只,只分离腹主动脉不结扎作为对照,余大鼠采用肾上腹主动脉缩窄法制作慢性心力衰竭(心衰)模型。8周后入选心衰大鼠随机分为心衰对照组(CHF-C,n=13)和心衰他汀干预组(CHF-S,n=13)2组。CHF-S组用5mg/(kg·d)辛伐他汀灌胃,CHF-C组、Sham组每d灌等量生理盐水。继续喂养4周后测量血流动力学参数;心室内采血,ELISA法测定IL-18水平。计算左心室质量指数(LVMI)。结果:和Sham组比较,CHF-C组血流动力学参数有明显差异(P均<0.05)。和CHF-C组相比,CHF-S组血流动力学参数有明显改善(P均<0.05)。Sham组、CHF-C组、CHF-S组IL-18水平分别为(271.50±64.75)ng/L,(581.20±67.71)ng/L和(505.95±60.29)ng/L,LVMI分别为(1.87±0.05)×10-3,(3.01±0.12)×10-3和(2.16±0.23)×10-3。和Sham组比较,CHF-C组IL-18升高,LVMI升高(P均<0.05)。和CHF-C组相比,CHF-S组IL-18降低,LVMI下降(P均<0.05)。结论:IL-18能够反映心衰的严重程度;辛伐他汀能够改善心衰的症状。

关键词：辛伐他汀充血性心力衰竭 IL-18 血流动力学大鼠

汇川MD880-50M系列逆变单元结构功能原理及维修维护介绍

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变频器世界 2024年第6期27卷 98-103页

作者：安宏亮日照钢铁有限公司

经过多年技术追赶,国内变频器企业在生产工艺上逐渐完善,产品可靠性稳步提升,国产品牌变频器市场占有量逐渐提高,未来随着国产品牌定制化能力以及产品研发制造实力的提升,国产替代趋势有望延续,汇川品牌变频器市场占有率最高,其可靠的性... 详细信息

经过多年技术追赶,国内变频器企业在生产工艺上逐渐完善,产品可靠性稳步提升,国产品牌变频器市场占有量逐渐提高,未来随着国产品牌定制化能力以及产品研发制造实力的提升,国产替代趋势有望延续,汇川品牌变频器市场占有率最高,其可靠的性能,完善的功能控制,得到市场肯定,其品牌在我单位应用领域也比较广泛。

关键词：逆变单元结构布局功能模块维修维护

人参皂甙Rb1对心力衰竭大鼠心肌缝隙连接蛋白43的影响

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天津医药 2013年第7期41卷 675-678页

作者：孔宏亮李占全苗志林叶丽萍辽宁省人民医院心脏中心 110016

目的探讨人参皂甙Rb1(Gs-Rb1)改善阿霉素(Dox)所致心力衰竭(HF)效应是否与调整缝隙连接蛋白43(CX43)有关以及调节CX43的可能机制。方法 Dox诱导的HF大鼠被随机分为Dox组(n=15)和Gs-Rb1组[70mg/(kg·d),n=17],同龄健康鼠作为对照组(n=10)... 详细信息

目的探讨人参皂甙Rb1(Gs-Rb1)改善阿霉素(Dox)所致心力衰竭(HF)效应是否与调整缝隙连接蛋白43(CX43)有关以及调节CX43的可能机制。方法 Dox诱导的HF大鼠被随机分为Dox组(n=15)和Gs-Rb1组[70mg/(kg·d),n=17],同龄健康鼠作为对照组(n=10);体外Dox干预的心肌细胞亦被随机分为Dox组、Gs-Rb1组和对照组。干预完成后,分别行心脏超声和流式细胞仪(FCM)检查;Westernbolt或RT-PCR检测p21蛋白活化激酶l(PAK1)、蛋白质磷酸酶-2A(PP2A)和CX43等的表达。结果 Gs-Rb1显著改善HF大鼠心功能、显著降低左室质量指数和显著降低Dox所致的心肌细胞凋亡。Dox组CX43mRNA和CX43蛋白显著低于对照组,而Gs-Rb1可显著升高CX43,但仍显著低于对照组。Dox组PAK1与对照组差异无统计学意义(P>0.05),而Gs-Rb1显著上调PAK1表达。Dox组PP2A的表达显著高于对照组,而Gs-Rb1组PP2A的表达显著高于Dox组。结论 Gs-Rb1通过调节CX43改善Dox的心肌损害效应,该效应可能受PAK1-PP2A调节。

关键词：人参皂甙心力衰竭连接蛋白类心肌蛋白质磷酸酶2 p21蛋白活化激酶1

丹参川芎嗪注射液联合西药治疗冠心病不稳定性心绞痛随机平行对照研究

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实用中医内科杂志 2016年第5期30卷 93-95页

作者：冯静李占全孔宏亮辽宁中医药大学第一临床学院沈阳110032 辽宁省人民医院心内科沈阳110000

[目的]观察丹参川芎嗪注射液联合西药治疗冠心病不稳定性心绞痛。[方法]使用随机平行对照方法,将520例住院患者按病志号抽签方法简单随机分两组。对照组260例拜阿司匹林,100mg/次,1次/d;硫酸氢氯吡格雷,75mg/次,1次/d;倍他乐克,12.5mg/... 详细信息

[目的]观察丹参川芎嗪注射液联合西药治疗冠心病不稳定性心绞痛。[方法]使用随机平行对照方法,将520例住院患者按病志号抽签方法简单随机分两组。对照组260例拜阿司匹林,100mg/次,1次/d;硫酸氢氯吡格雷,75mg/次,1次/d;倍他乐克,12.5mg/次,2次/d;单硝酸异山梨酯,40mg/次,1次/d;阿托伐他汀钙,20mg/次,每晚1次,睡前口服;盐酸曲美他嗪,30mg/次,3次/d;左卡尼汀2.0g+氯化钠250mL,静点。治疗组260例丹参川芎嗪注射液10mL+氯化钠250mL,静点;西药治疗同对照组。连续治疗14d为1疗程。观测临床症状、心电图、不良反应。治疗1疗程,判定疗效。[结果]症状疗效、心电图疗效治疗组优于对照组(P<0.01)。[结论]丹参川芎嗪注射液联合西药治疗冠心病不稳定性心绞痛,疗效满意,无严重不良反应,值得推广。

关键词：冠心病不稳定性心绞痛胸痹丹参川芎嗪注射液拜阿司匹林硫酸氢氯吡格雷倍他乐克单硝酸异山梨酯阿托伐他汀钙盐酸曲美他嗪左卡尼汀心电图中西医结合治疗随机平行对照研究