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Interferon-gamma primes macrophage activation by increasing PTEN via downregulation of miR-3473b

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Interferon-gamma primes macrophage activation by increasing ...

英国高校科研影响力评估的改革与启示--基于REF2021的分析

第九届全国免疫学学术大会

作者： Cong Wu Mingkun Chen hongli yan Anmei Deng Department of Laboratory Diagnosis Changhai Hospital affiliated to Second Military Medical University

The classical activation of macrophages,one of major innate effector cells,requires interferon-γ(IFN-γ)pretreatment(prim-ing)and subsequent TLR stimuli(triggering).The priming effect of IFN-γcan promote macrophages to secrete higher levels of pro-inflammatory cytokines but lower level of the anti-inflammatory cytokines,enhancing

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外国教育研究 2021年第7期48卷 58-72页

作者：王梅颜红丽天津大学教育学院、教育科学研究中心天津300354 天津大学教育学院天津300354

作为知识创新和科学发现的重要阵地,科学研究对经济社会发展的影响以及科研影响力评价已经引起各国高等教育工作者的广泛关注。英国高校科研影响力评价水平在国际上一直处于领先地位。自2019年1月开始,英国高等教育基金管理委员会陆续... 详细信息

作为知识创新和科学发现的重要阵地,科学研究对经济社会发展的影响以及科研影响力评价已经引起各国高等教育工作者的广泛关注。英国高校科研影响力评价水平在国际上一直处于领先地位。自2019年1月开始,英国高等教育基金管理委员会陆续发布“科研卓越框架2021”的评估指南、评估标准等文件进一步改革和完善科研影响力评估,增加科研影响力的评估比重,将科研对教学和学生活动的影响纳入评估范围,并且新增公众参与和教学的影响类别。对“科研卓越框架2021”中关于科研影响力评估的内容及其改革的分析,可以为我国开展科研影响力评估和提升科研服务水平提供参考与借鉴。

关键词：英国科研卓越框架科研影响力评估

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外泌体治疗宫腔粘连的研究进展

中华生殖与避孕杂志 2024年第1期44卷 83-89页

作者：鄢然刘苡萱颜宏利海军军医大学基础医学院学员一大队上海200433 海军军医大学第一附属医院生殖医学中心上海200433

外泌体是一类细胞外囊泡,是近年来子宫内膜损伤后再生与修复领域的研究热点。外泌体可由多种细胞释放,能通过miRNA等多种生物活性物质调节受体细胞功能,从而有效促进组织的再生修复。宫腔粘连(intrauterine adhesions,IUA)是一种常见的... 详细信息

外泌体是一类细胞外囊泡,是近年来子宫内膜损伤后再生与修复领域的研究热点。外泌体可由多种细胞释放,能通过miRNA等多种生物活性物质调节受体细胞功能,从而有效促进组织的再生修复。宫腔粘连(intrauterine adhesions,IUA)是一种常见的由于子宫创伤、感染、遗传因素等导致子宫内膜基底层受损的子宫内膜损伤性疾病,常因宫腔部分或全部闭塞而引起月经异常、不孕或反复流产等临床问题,是女性生育困难原因中不可忽视的一部分,并且现有的治疗措施无法实现满意的子宫内膜损伤后修复效果。因此,近年来有研究对外泌体促进IUA中内膜修复、生育力恢复的作用进行新探索,证明了外泌体修复内膜损伤的有效性,并逐渐摸索出其中的作用机制。外泌体凭借抑制细胞凋亡和上皮间质转化及纤维化、促进细胞增殖和血管生成、参与免疫调控等作用,可增加受损内膜的厚度和腺体数量,并提高子宫内膜容受性及生育力,将来有望成为IUA的新型治疗手段。本文将对外泌体治疗IUA的研究进展进行综述。

关键词：外泌体上皮间质转化宫腔粘连

DNA Methylomes in CpG Island Methylator Phenotype(CIMP) Colon Cancer:new screening marks proposal

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DNA Methylomes in CpG Island Methylator Phenotype(CIMP) Colo...

Silencing of Grainyhead-like-2(GRHL2) at the tumor edge by DNA methylation facilitates Epithelial-Mesenchymal Transition through targeting Rab25 and E-cadherin

第十二次全国医学遗传学学术会议

作者： hongli yan Guan’nan Tang Yiliang Zhang Yuzhao Wang Shuhan SUN Department of medical genetics Second Military Medical University

Background & Aims:DNA methylation is essential for normal development and is frequently altered in *** Island Methylator Phenotype(CIMP) is a subset of colorectal cancers was postulated to have a higher propensity for DNA ***,the validity of CIMP,its molecular basis,and its prognostic values are highly *** aims of this study were to(1) compare and characterize the unique features of DNA methylomes of CIMP with Non-CIMP colonrectal cancers;(2) propose a new panel of valuable markers for CIMP screening. Methods & Results:Here we utilized MBD-isolated Genome Sequencing(MiGS) to map the DNA methylomes of three sets of normal colon,non-CIMP colon cancers,and CIMP colon *** regions only robust hypermethylated in CIMP tumors were evaluated in 120 colorectal tumors by Bisulfite genomic sequencing or Methylation Specific PCR(MSP).We found 4,800 sites were hypermethylated in both non-CIMP and CIMP colon cancers when compared with normal *** those sites,318 sites were robust hypermethylated in CIMP tumors only. Methylation of these CpG islands regulates 122 key genes in tumorgenesis and progress.A panel of 5 new markers was proposed for CIMP screening in 120 consecutive colon samples,13.3% (16/120) were methylated in all 5 markers and 87.5%(14/16) were *** the markers described by Weisenberger et al.,22.5%(27/120) were methylated in all 5 markers and only 51.8%(14/27) were MSI ***:CIMP are a subset of colorectal cancer has unique features in DNA *** genes only hypermethylated in CIMP tumors shed lights on exploration the mechanism of CIMP tumorgenesis and provide new targets for cancer therapies. The new panel of markers facilitates CIMP screening in sporadic colorectal cancers.

关键词： Colorectal cancer CpG Island Methylator Phenotype MBD-isolated Genome Sequencing DNA methylome

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Silencing of Grainyhead-like-2(GRHL2) at the tumor edge by D...

Effects of Trichostatin A on HDAC8 Expression,Proliferation and Cell Cycle of Molt-4 Cells

第十二次全国医学遗传学学术会议

作者： Guan’nan Tang hongli yan Yiliang Zhang YuZhao Wang Shuhan SUN Department of medical genetics Second Military Medical University

Background & Aims:Epithelial-Mesenchymal Transition(EMT) plays a pivotal role in tumor invasion and *** it is well documented that EMT occurred predominantly at the tumor edge where it is induced by cytokines,the extracellular matrix environment,or hypoxia,the mechanism(s) underlying the tumor microenvironment-induced Epithelial-Mesenchymal Transition remains ***-like-2(GRHL2) is a new transcription factor involved in embryo development and epithelial *** of GRHL2 has been noted in several tumor types,including breast cancer,colon *** addition,it has been reported that GRHL2 regulated the epithelial apical junctional complex by targeting the tight junction gene claudin 4(Cldn4).These findings imply that GRHL2 might play a key role in Epithelial-Mesenchymal *** & Results:We systemically analyzed the expression of GRHL2 in colon and liver cancer *** control by querying oncomine database and real time PCR analysis,the resulted showed that GRHL2 was respectively down-regulated in 40%and 76%of colon cancer and liver *** investing the expression of GRHL2 in colon cancer by immunohistochemistry analysis indicated that GRHL2 was loss of expression at the tumor edge and surround fibroblast *** further found that promoter of GRHL2 was embedded in a large CpG island and methylated in tumor cells with a low level of *** by 5-aza-dc treatment significantly enhanced GRHL2 expression in SW480 and SW620 cells,revealed that GRHL2 is epigenetically silenced by DNA methylation. Overexprssion of GRHL2 in SW480 and SW620 promotes Epithelial-Mesenchymal Transition (MET).Real time PCR,Western blot and Immunoprecipitation(ChIP) assay indicated that over-expression of GRHL2 enhances E-cadherin and Rab25 expression by binding to the promoter of these two *** addition,we also found that overexpression of GRHL2 suppresses CD24 ***:These data indicated that

关键词： Colorectal cancer Liver cancer Grainyhead-like-2(GRHL2) DNA methylation Epithelial-Mesenchymal Transition(EMT)

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2006年第5期26卷 531-533页

作者：何静刘红利陈燕 Department of Hematology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

The effects of Trichostatin A （TSA） on histone deacetylase 8 （HDAC8） expression, proliferation and cell cycle arrest in T-lymphoblastic leukemia cell line Molt-4 cells in vitro were investigated. The effect of TSA on the growth of Molt-4 cells was studied by MTT assay. Flow cytometry was used to examine the cell cycle. The expression of HDAC8 was detected by using immunocytochemistry and Western blot. The results showed that proliferation of Molt-4 cells was inhibited in TSA-treated group in a time- and dose-dependent manner, The IC50 of TSA exposures for 24 h and 36 h were 254.3236 and 199.257 μg/L respectively. The cell cycle analysis revealed that Molt-4 was mostly in G0/G1 phase, and after treatment with TSA from 50 to 400 μg/L for 24 h, the percents of G0/G1 cells were decreased and cells were arrested in GJM phase. Treatment of TSA for 24 h could significantly inhibit the expression of HDAC8 protein in Molt-4 cells （P〈0.01）. It was concluded that TSA could decrease the expression of HDAC8 in Molt-4 cells, which contributed to the inhibition of proliferation and induction of cell cycle arrest in Molt-4 cells.

关键词： trichostain A histone deacetylase 8 T-lymphoblastic leukemia Molt-4

循环肿瘤细胞检测研究进展:从单纯计数到表型与功能分析

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中国肿瘤生物治疗杂志 2017年第4期24卷 341-347页

作者：毕峰瑞颜宏利第二军医大学附属长海医院实验诊断科上海200433

随着靶向治疗、免疫治疗等新技术的发展,肿瘤诊疗已经步入精准医疗的新时代。液体活检作为一种便捷、无创、能够动态反映肿瘤基因谱全貌的方法在肿瘤精准医疗中发挥着越来越重要的作用。血液循环肿瘤细胞(circulating tumor cell,CTC)... 详细信息

随着靶向治疗、免疫治疗等新技术的发展,肿瘤诊疗已经步入精准医疗的新时代。液体活检作为一种便捷、无创、能够动态反映肿瘤基因谱全貌的方法在肿瘤精准医疗中发挥着越来越重要的作用。血液循环肿瘤细胞(circulating tumor cell,CTC)检测是最早开展的液体活检技术,在肿瘤早期诊断、预后判断、疗效评估等方面获得了广泛应用。初级阶段CTC的检测以单纯计数为主,但人们逐渐发现单纯计数无法真实反映肿瘤的负荷及变化情况。随着单细胞测序等技术的不断发展,人们对CTC的认识也不断深入,逐渐转向以分子表型和功能分析为主的高级阶段。本文结合近年来的临床实践,重点阐述CTC检测从初级阶段发展到高级阶段及其在肿瘤精准医疗中应用的研究进展。

关键词：循环肿瘤细胞液体活检细胞计数分子表型功能分析

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图书馆联盟资源状况与绩效的关系分析

图书馆 2011年第4期 106-107页

作者：党洪莉鄢朝晖湖南师范大学图书馆湖南长沙410081

图书馆联盟的广泛兴起,引起了学术界对联盟的研究兴趣,研究方向包括图书馆联盟运行机制、图书馆联盟绩效评估等多个理论。本文分析联盟合作方的资源在整个联盟中所起作用,侧重于从资源特征、资源组合角度研究联盟的绩效。

关键词：图书馆联盟资源资源组合绩效

稳定期慢性阻塞性肺疾病患者久坐行为与生命质量之间的关系:衰弱的中介作用

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中国实用护理杂志 2024年第16期40卷 1244-1251页

作者：邹旭何绍华闫红丽上海市第十人民医院崇明分院呼吸危重医学科上海202157 海军军医大学第一附属医院上海长海医院急诊科上海200433

目的探讨衰弱在稳定期慢性阻塞性肺疾病(COPD)患者久坐行为与生命质量之间的中介作用,为改善稳定期COPD患者的生命质量提供实证依据。方法采用便利抽样法选取2022年1月至2023年2月上海市第十人民医院崇明分院门诊就诊的228例稳定期COPD... 详细信息

目的探讨衰弱在稳定期慢性阻塞性肺疾病(COPD)患者久坐行为与生命质量之间的中介作用,为改善稳定期COPD患者的生命质量提供实证依据。方法采用便利抽样法选取2022年1月至2023年2月上海市第十人民医院崇明分院门诊就诊的228例稳定期COPD患者为研究对象,使用一般资料调查表、国际体力活动问卷、Tilburg衰弱量表、COPD评估测试问卷进行横断面调查。采用结构方程模型分析衰弱在久坐行为与生命质量之间的中介作用,Bootstrap法分析中介效应值。结果回收有效问卷219份,有效回收率为96.05%(219/228)。219例稳定期COPD患者,男168例,女51例,年龄69(62,74)岁。稳定期COPD患者久坐时间为6(5,8)h/d,衰弱得分为5(3,7)分,生命质量得分为(13.56±2.56)分。Pearson相关性分析显示,稳定期COPD患者久坐时间、衰弱与生命质量呈正相关(r=0.420、0.582,均P<0.01),且久坐时间与衰弱也呈正相关(r=0.698,P<0.01);Bootstrap法检验表明,久坐时间的直接效应为0.228(95%CI 0.082~0.374),以衰弱为中介的间接效应为0.169(95%CI 0.080~0.215),总效应为0.397,直接效应与中介效应的95%CI均不包含0,此模型为部分中介模型,中介效应占比为42.57%。结论稳定期COPD患者的生命质量处于中度水平,久坐行为不仅能够直接影响生命质量,还可以通过引起或加重衰弱,间接影响生命质量。临床工作中应鼓励患者通过站立、经常改变体位或进行轻度体力活动等方式避免因长时间久坐而引起或加重衰弱,以改善稳定期COPD患者的生命质量。

关键词：肺疾病,慢性阻塞性生活质量久坐行为衰弱中介稳定期