检索结果-南通市图书馆

NON-TOPOLOGICAL SOLITON IN NUCLEAR MEDIUM

在线全文

学校读者我要写书评

暂无评论

Chinese Physics Letters 1990年第8期7卷 341-344页

作者： ZHANG Jianwei jin hongli QIU Xijun Institute of Nuclear Research Academia SinicaShanghai 201849 On leave from Inner-Mongonia Teachers College for the Nationalities

The bag constant and the size increment of a nucleon(soliton)embedded in nuclear medium,are calculated in a non-topological soliton *** results may be understood as arising from the change of the vacuum properties in ... 详细信息

关键词： model. soliton increment

基于UPLC定量指纹图谱与UPLC-QE-Plus-MS/MS技术的吴茱萸(石虎)标准汤剂量值传递研究

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中草药 2024年第5期55卷 1541-1552页

作者：甘佳攀张旗陆绍铭何思思肖柳君许妍谭灵芝薛倩倩金红利赣江中药创新中心江西南昌330000 江西省中药药效物质基础重点实验室赣江中药创新中心江西南昌330000 中国科学院大连化学物理研究所辽宁大连116023 江西省药品检验检测研究院江西南昌330000 江西百神药业股份有限公司江西省中药配方颗粒重点实验室江西宜春330600

目的借助UPLC定量指纹图谱与UPLC-QE-Plus-MS/MS技术,研究吴茱萸Euodiae Fructus[石虎Euodia rutaecarpa(Juss.)***(Dode)Huang]标准汤剂的量值传递规律。方法通过对18批吴茱萸(石虎)样品进行检测,获得吴茱萸(石虎)饮片和标准汤剂的对... 详细信息

目的借助UPLC定量指纹图谱与UPLC-QE-Plus-MS/MS技术,研究吴茱萸Euodiae Fructus[石虎Euodia rutaecarpa(Juss.)***(Dode)Huang]标准汤剂的量值传递规律。方法通过对18批吴茱萸(石虎)样品进行检测,获得吴茱萸(石虎)饮片和标准汤剂的对照指纹图谱,并采用UPLC-QE-Plus-MS/MS技术,对标定的吴茱萸(石虎)共有峰进行鉴定。通过峰个数传递、相似度比较、有效成分含量转移率、出膏率测定等,对吴茱萸(石虎)标准汤剂的量值传递规律进行研究。结果在饮片与标准汤剂中共标定25个对照指纹图谱共有峰,包括5个生物碱类化合物(去氢吴茱萸碱、evodiaxinine、吴茱萸酰胺、吴茱萸碱、吴茱萸次碱)、12个有机酸类化合物(咖啡酰葡萄糖酸及其同分异构体、阿魏酰葡萄糖酸及其同分异构体、绿原酸、新绿原酸、隐绿原酸、阿魏酰奎宁酸等)、7个黄酮类化合物(香树素-7-O-β-D-葡萄糖苷、芦丁、金丝桃苷、柠檬黄素-3-O-芸香糖苷、柯伊利素-7-O-芸香糖苷、水仙苷、异鼠李素-3-O-β-D-葡萄糖苷)、1个柠檬苦素类化合物(柠檬苦素)。经测定饮片中25个共有峰均传递到标准汤剂中,共有峰传递率为100%。以每批次吴茱萸(石虎)饮片的指纹图谱为对照,对应批次标准汤剂的指纹图谱相似度均大于0.9。以有效成分柠檬苦素、吴茱萸碱、吴茱萸次碱为指标,吴茱萸(石虎)标准汤剂中指标成分的转移率均在其均值±30%范围内。18批标准汤剂的出膏率也在其均值±30%范围内。结论制备过程量值传递情况稳定,制备工艺可行,可为吴茱萸(石虎)配方颗粒的质量标准研究提供依据。

关键词： UPLC 定量指纹图谱 UPLC-QE-Plus-MS/MS技术吴茱萸石虎标准汤剂量值传递柠檬苦素吴茱萸碱吴茱萸次碱 evodiaxinine 吴茱萸酰胺咖啡酰葡萄糖酸阿魏酰葡萄糖酸绿原酸新绿原酸隐绿原酸阿魏酰奎宁酸香树素-7-O-β-D-葡萄糖苷芦丁金丝桃苷柠檬黄素-3-O-芸香糖苷柯伊利素-7-O-芸香糖苷水仙苷异鼠李素-3-O-βD-葡萄糖苷

Genetically engineered bacterial-like particles induced specific cellular and humoral immunity as effective tick-borne encephalitis virus vaccine

在线全文

学校读者我要写书评

暂无评论

聚集体(英文)

聚集体(英文) 2023年第3期4卷 175-183页

作者： Mengyao Zhang hongli jin Yuanyuan Li Cuicui Jiao Pei Huang Yujie Bai Zhiyuan Gong Haili Zhang Shunjie Liu Hualei Wang Key Laboratory of Zoonosis Research Ministry of EducationCollege of Veterinary MedicineJilin UniversityChangchunChina

Tick-borne encephalitis(TBE)is a natural focal disease with fatal encephalitis induced by tick-borne encephalitis virus(TBEV),seriously threatening human and public *** of TBE depends on vaccination with inactivated vaccine,which requires high cost and multiple ***,we construct genetically engineered bacterial-like particles(BLPs)as an effective TBEV vaccine with simplified immunizations and improved immune *** TBEV BLPs involve the combination of the gram-positive enhancer matrix from Lactococcus lactis,and TBEV envelope(E)protein expressed by genetically engineered recombinant *** prepared TBEV BLPs can effectively stimulate the activation of dendritic cells to present the TBEV E proteins to T and B cells,leading to strong and durable cellular and humoral immune responses in ***,the serum levels of specific IgG antibodies in mice remain about 10^(6)at 6 months after the secondary ***,the TBEV BLPs can be used as a potent vaccine candidate,laying the foundation for developing novel TBEV genetically engineered vaccines.

关键词： bacterial-like particles envelope protein genetically engineered vaccines gram-positive enhancer matrix tick-borne encephalitis virus

检测寨卡病毒核酸的RT-LAMP可视化方法的建立

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国兽医学报 2024年第8期44卷 1698-1703页

作者：宋雨濛黄培金宏丽焦翠翠白玉洁张梦瑶龚志远李媛媛张海丽王化磊吉林大学动物医学学院人畜共患传染病重症诊治全国重点实验室/教育部人兽共患病研究重点实验室吉林长春130062 长春西诺生物科技有限公司吉林长春130012

为建立简单、便捷、灵敏、特异的寨卡病毒(Zika virus,ZIKV)快速检测方法,本研究通过对不同时间、不同地区分离的ZIKV全基因组序列进行分析,筛选ZIKV NS5基因保守区为靶标,同时设计特异性引物及探针,结合RT-LAMP恒温扩增技术和免疫层析... 详细信息

为建立简单、便捷、灵敏、特异的寨卡病毒(Zika virus,ZIKV)快速检测方法,本研究通过对不同时间、不同地区分离的ZIKV全基因组序列进行分析,筛选ZIKV NS5基因保守区为靶标,同时设计特异性引物及探针,结合RT-LAMP恒温扩增技术和免疫层析技术,成功构建ZIKV反转录环介导等温扩增核酸可视化(RT-LAMP-VF)检测方法。结果显示,该方法具有良好的特异性和敏感性,当内、外、环引物比为FIP∶LF∶F3=4∶2∶1时,在61℃条件下恒温扩增45 min,可特异性检测到10^(2)copies/μL的RNA标准品以及2.15 pfu的ZIKV,且与其他黄病毒(日本乙型脑炎病毒、猪瘟病毒)等无交叉反应。血液组织中的其他RNA不影响RT-LAMP-VF的敏感性和特异性,表明该方法可用于临床样本ZIKV的检测。本研究建立的ZIKV RT-LAMP-VF检测方法操作简单、不需要特殊仪器设备,尤其适用于基层单位ZIKV的现场快速检测,为ZIKV病的现场快速检测和预警预报体系的建立提供技术支持与物质保障。

关键词：寨卡病毒环介导等温扩增免疫层析试纸条核酸可视化检测

Predict the evolution of mechanical property of Al-Li alloys in a marine environment

在线全文

学校读者我要写书评

暂无评论

Defence Technology（防务技术） 2024年第1期31卷 557-566页

作者： Wei Li Lin Xiang Guang Wu hongli Si jinyan Chen Yiming jin Yan Su Jianquan Tao Chunyang Huang State Key Laboratory of Explosion Science and Technology Beijing Institute of TechnologyBeijing100036China Chongqing Innovation Center Beijing Institute of TechnologyChongqing400044China First Research Institute of the Ministry of Public Security for PR China Beijing100036China Southwest Technology and Engineering Research Institute Chongqing400039China

The ocean is one of the essential fields of national defense in the future,and more and more attention is paid to the lightweight research of Marine equipment and *** study it is to develop a Machine learning(ML)-based prediction method to study the evolution of the mechanical properties of Al-Li alloys in the marine *** obtained the mechanical properties of Al-Li alloy samples under uniaxial tensile deformation at different exposure times through Marine exposure *** obtained the strain evolution by digital image correlation(DIC).The strain field images are voxelized using 2D-Convolutional Neural Networks(CNN)autoencoders as input data for Long Short-Term Memory(LSTM)neural ***,the output data of LSTM neural networks combined with corrosion features were input into the Back Propagation(BP)neural network to predict the mechanical properties of Al-Li *** main conclusions are as follows:*** variation law of mechanical properties of2297-T8 in the Marine atmosphere is *** the increase in outdoor exposure test time,the tensile elastic model of 2297-T8 changes slowly,within 10%,and the tensile yield stress changes significantly,with a maximum attenuation of 23.6%.*** prediction model can predict the strain evolution and mechanical response simultaneously with an error of less than 5%.*** study shows that a CNN/LSTM system based on machine learning can be built to capture the corrosion characteristics of Marine exposure *** results show that the relationship between corrosion characteristics and mechanical response can be predicted without considering the microstructure evolution of metal materials.

关键词： Marine environment Al-Li alloy Machine learning Corrosion

A visual assay panel for the identification of monkeypox virus DNA belonging to the clades I and II

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2023年第4期38卷 635-638页

作者： Pei Huang Zanheng Huang Meihui Liu Yujie Bai hongli jin jingbo Huang Xingqi Liu Zhenhong Guan Ming Duan Haili Zhang Yuanyuan Li Sandra Chiu Hualei Wang State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases Key Laboratory for Zoonosis Research of the Ministry of EducationInstitute of ZoonosisCollege of Veterinary MedicineJilin UniversityChangchun130062China Division of Life Sciences and Medicine University of Science and Technology of ChinaHefei244199China

Dear Editor,Monkeypox virus(MPXV)is an enveloped double-stranded DNA virus belonging to the family Poxviridae,subfamily Chordopoxvirinae,and genus Orthopoxvirus(Hraib et al.,2022;Gong et al.,2022).MPXV forms Congo Basin clade(clade I)and West African clade(clade II)(Durski et al.,2018).Additionally,clade II consists of two subclades,clade IIa and clade IIb.

关键词： belong panel African

An inactivated recombinant rabies virus chimerically expressed RBD induces humoral and cellular immunity against SARS-CoV-2 and RABV

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Virologica Sinica 2023年第2期38卷 244-256页

作者： Haili Zhang hongli jin Feihu Yan Yumeng Song Jiaxin Dai Cuicui Jiao Yujie Bai jingxuan Sun Di Liu Shen Wang Mengyao Zhang Jilong Lu jingbo Huang Pei Huang Yuanyuan Li Xianzhu Xia Hualei Wang Key Laboratory of Zoonosis Research Ministry of EducationCollege of Veterinary MedicineJilin UniversityChangchun130062China Changchun Veterinary Research Institute Chinese Academy of Agricultural SciencesChangchun130122China Changchun Sino Biotechnology Co. Ltd.Changchun130012China

Many studies suggest that severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)can infect various animals and transmit among animals,and even to humans,posing a threat to humans and *** is an urgent need to develop inexpensive and efficient animal vaccines to prevent and control coronavirus disease 2019(COVID-19)in *** virus(RABV)is another important zoonotic pathogen that infects almost all warmblooded animals and poses a great public health *** present study constructed two recombinant chimeric viruses expressing the S1 and RBD proteins of the SARS-CoV-2 Wuhan01 strain based on a reverse genetic system of the RABV SRV9 strain and evaluated their immunogenicity in mice,cats and *** results showed that both inactivated recombinant viruses induced durable neutralizing antibodies against SARS-CoV-2 and RABV and a strong cellular immune response in ***,inactivated SRV-nCoV-RBD induced earlier antibody production than SRV-nCoV-S1,which was maintained at high levels for longer *** SRV-nCoV-RBD induced neutralizing antibodies against both SARS-CoV-2 and RABV in cats and dogs,with a relatively broadspectrum cross-neutralization capability against the SARS-CoV-2 pseudoviruses including Alpha,Beta,Gamma,Delta,and Omicron,showing potential to be used as a safe bivalent vaccine candidate against COVID-19 and rabies in animals.

关键词： SARS-CoV-2 Vaccine RBD Chimeric expression RABV

发热伴血小板减少综合征病毒Gn蛋白DⅢ-Ⅲ的表达与抗体间接ELISA检测方法的建立

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国兽医学报 2024年第8期44卷 1704-1712页

作者：张梦瑶梁天来闫飞虎陈韬焦翠翠金宏丽栾娇彦吴晓黄培张海丽宁琴王化磊李媛媛吉林大学动物医学学院人畜共患传染病重症诊治全国重点实验室吉林长春130062 中国农业科学院长春兽医研究所吉林长春130028 华中科技大学同济医学院附属同济医院感染科湖北武汉430030

利用PCR技术扩增发热伴血小板减少综合征病毒(severe fever with thrombocytopenia syndrome virus,SFTSV)Gn-DⅢ-Ⅲ基因,将其插入pET-30a(+)原核表达载体,构建重组质粒pET-SFTSV-Gn-DⅢ-Ⅲ,将测序正确的pET-SFTSV-Gn-DⅢ-Ⅲ转化至大肠杆菌BL21(DE3)中,优化Gn-DⅢ-Ⅲ蛋白表达表达条件。利用镍柱亲和层析法纯化的Gn-DⅢ-Ⅲ蛋白作为包被抗原,建立检测SFTSV抗体的间接ELISA方法,并进行评价。结果显示,通过PCR和测序鉴定重组质粒pET-SFTSV-Gn-DⅢ-Ⅲ构建成功;重组Gn-DⅢ-Ⅲ蛋白为可溶性表达,其最佳诱导条件为:0.4 mmol/L IPTG于25℃诱导4 h;经镍柱纯化后蛋白纯度高达91.77%;SFTSV抗体间接ELISA检测方法的的最佳反应条件为:5 mg/L的包被质量浓度,一抗在37℃孵育1.5 h,二抗1∶10000稀释后在37℃孵育1 h。特异性试验结果显示该方法与裂谷热病毒(Rift Valley fever virus;RVFV)、埃博拉病毒(Ebola virus,EBOV)和蜱传脑炎病毒(tick-borne encephalitis virus,TBEV)阳性血清均无交叉反应;敏感性试验结果显示该方法有较高的敏感性,SFTSV阳性血清稀释至81920倍时其P/N仍大于2.1;重复性结果表明批内和批间反应变异系数均小于10%。应用所建立方法检测了4份人类感染SFTSV不同阶段的临床血清样本,结果缓解期患者血清P/N值大于2.1,为阳性,多器官衰竭期患者血清P/N值小于2.1,为阴性。结果表明,本研究成功表达并纯化了SFTSV Gn-DⅢ-Ⅲ蛋白,并以此为包被蛋白建立SFTSV抗体间接ELISA检测方法,该方法具有良好的特异性、敏感性和重复性,可用于检测人类SFTSV临床血清样本。

关键词：发热伴血小板减少综合征病毒 Gn蛋白DomainⅢ 原核表达间接ELISA

The Application of a Safe Neutralization Assay for Ebola Virus Using Lentivirus-Based Pseudotyped Virus

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2021年第6期36卷 1648-1651页

作者： Zengguo Cao hongli jin Gary Wong Ying Zhang Cuicui Jiao Na Feng Fangfang Wu Shengnan Xu Hang Chi Yongkun Zhao Tiecheng Wang Weiyang Sun Yuwei Gao Songtao Yang Xianzhu Xia Hualei Wang Key Laboratory of Zoonosis Research Ministry of EducationCollege of Veterinary MedicineJilin UniversityChangchun 130062China Key Laboratory of Jilin Province for Zoonosis Prevention and Control Institute of Military VeterinaryAcademy of Military Medical SciencesChangchun 130122China Institut Pasteur of Shanghai Chinese Academy of SciencesShanghai 200031China Département de Microbiologie-Infectiologie Et D'immunologie UniversitéLavalQuébecQC G1V 0A6Canada Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonose Yangzhou UniversityYangzhou 225009China

Dear Editor,Zaire Ebola virus(EBOV)is a negative-sense singlestranded RNA virus that is capable of causing acute hemorrhagic fever with a frightening fatality rate that can reach up to 90%.Due to its high lethality and frequent recurrence,EBOV is a substantial threat to public *** about live EBOV is restricted to BSL-4 laboratories;therefore,conventional serological detection methods do not provide a safe way to evaluate neutralizing activity,resulting in a bottleneck in antibody-based detection of viruses such as ***,we aim to develop and apply a neutralization assay for EBOV using a generated lentivirus-based pseudotyped EBOV bearing GP on its surface.

关键词： Ebola acute hemorrhagic