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Karyotype Stability and Unbiased Fractionation in the Paleo-Allotetraploid Cucurbita Genomes

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Molecular Plant 2017年第10期10卷 1293-1306页

作者： honghe.sun Shan WU Guoyu Zhang Chen Jiao Shaogui Guo Yi Ren Jie Zhang Haiying Zhang Guoyi Gong Zhangcai Jia Fan Zhang Jiaxing Tian William J. Lucas Jeff J. Doyle Haizhen Li Zhangjun Fei Yong Xu National Engineering Research Center for Vegetables Beijing Academy of Agriculture and Forestry Sciences Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China) Beijing Key Laboratory of Vegetable Germplasm Improvement Beijing 100097 China Boyce Thompson Institute Cornell University Ithaca NY 14853 USA Department of Plant Biology College of Biological Sciences University of California Davis CA 95616 USA Section of Plant Breeding & Genetics School of Integrated Plant Sciences Cornell University Ithaca NY 14853 USA USDA-ARS Robert W. Holley Center for Agriculture and Health Ithaca NY 14853 USA

The Cucurbita genus contains several economically important species in the Cucurbitaceae family. Here, we report high-quality genome sequences of C. maxima and C. moschata and provide evidence supporting an allotetraploidization event in Cucurbita. We are able to partition the genome into two homoeologous subgenomes based on different genetic distances to melon, cucumber, and watermelon in the Benincaseae tribe. We estimate that the two diploid progenitors successively diverged from Benincaseae around 31 and 26 million years ago （Mya）, respectively, and the allotetraploidization happened at some point between 26 Mya and 3 Mya, the estimated date when C. maxima and C. moschata diverged. The subgenomes have largely maintained the chromosome structures of their diploid progenitors. Such long-term karyotype stability after polyploidization has not been commonly observed in plant polyploids. The two subgenomes have retained similar numbers of genes, and neither subgenome is globally dominant in gene expression. Allele-specific expression analysis in the C. maxima ×C. moschata interspecific F1 hybrid and their two parents indicates the predominance of trans-regulatory effects underlying expression divergence of the parents, and detects transgressive gene expression changes in the hybrid correlated with heterosis in important agronomic traits. Our study provides insights into polyploid genome evolution and valuable resources for genetic improvement of cucurbit crops.

关键词： Cucurbita maxima Cucurbita moschata paleo-allotetraploid karyotype stability unbiased fractionation allele-specific expression

iTAK： A Program for Genome-wide Prediction and Classification of Plant Transcription Factors, Transcriptional Regulators, and Protein Kinases

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Molecular Plant 2016年第12期9卷 1667-1670页

作者： Yi Zheng Chen Jiao honghe.sun Hernan G. Rosli Marina A. Pombo Peifen Zhang Michael Banf Xinbin Dai Gregory B. Martin James J. Giovannoni Patrick X. Zhao Seung Y. Rhee Zhangjun Fei Boyce Thompson Institute Ithaca NY 14853 USA Instituto de Fisiología Vegetal (INFIVE CONICET-UNLP) La Plata 1900 Argentina Department of Plant Biology Carnegie Institution for Science Stanford CA 94305 USA Samuel Roberts Noble Foundation Ardmore OK 73401 USA Section of Plant Pathology and Plant-Microbe Biology School of Integrative Plant Science Cornell University Ithaca NY 14853 USA USDA-ARS Robert W. Holley Center for Agriculture and Health Ithaca NY 14853 USA

Dear Editor,Transcription factors （TFs） are proteins that regulate the expression of target genes by binding to specific cis-elements in pro-moter regions. Transcriptional regulators （TRs） a）so regulate the expression of target genes; however, they operate indirectly via interaction with the basal transcription apparatus （e.g., TFs）, or by altering the accessibility of DNA to TFs via chromatin remodeling. Another type of regulatory proteins, protein kinases （PKs）,

关键词： Mystery Mendel's Magnesium

The monkey microbial biobank brings previously uncultivated bioresources for nonhuman primate and human gut microbiomes

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mLife 2022年第2期1卷 210-217页

作者： Danhua Li Chang Liu Rexiding Abuduaini Mengxuan Du Yujing Wang Haizhen Zhu honghe Chen Nan Zhou Yuhua Xin Linhuan Wu Juncai Ma Yuguang Zhou Yong Lu Chengying Jiang Qiang sun Shuang-Jiang Liu State Key Laboratory of Microbial Resources and Environmental Microbiology Research Center at Institute of Microbiology Chinese Academy of SciencesBeijingChina College of Life Science University of Chinese Academy of SciencesBeijingChina State Key Laboratory of Microbial Biotechnology Shandong UniversityQingdaoChina China General Microorganism Culture Collection Institute of MicrobiologyChinese Academy of SciencesBeijingChina Microbial Resources and Big Data Center Institute of MicrobiologyChinese Academy of SciencesBeijingChina State Key Laboratory of Neuroscience CAS Key Laboratory of Primate NeurobiologyCAS Center for Excellence in Brain Science and Intelligence TechnologyChinese Academy of SciencesInstitute of NeuroscienceShanghaiChina

Impact statement Nonhuman primates(NHPs)such as monkeys are the closest living relatives to humans and are the best available models for causative studies of human health and *** microbiomes are intensively involved in host *** this study,by large-scale cultivation of microbes from fecal samples of monkeys,we obtained previously uncultured bacterial species and constructed a Macaca fascicularis Gut Microbial Biobank(MfGMB).The MfGMB consisted of 250 strains that represent 97 species of 63 genera,25 families,and 4 *** information of the 250 strains and the genomes of 97 cultured species are publicly *** MfGMB represented nearly 50% of core gut microbial compositions at the genus level and covered over 80% of the KO-based known gut microbiome functions of *** mining showed that the bacterial species in the MfGMB were prevalent not only in NHPs gut microbiomes but also in human gut *** study will help the understanding and future investigations on how gut microbiomes interact with their mammalian hosts.

关键词： statement monkey gut

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面向音频应用的可配置片上系统的设计与实现

微电子学 2009年第6期39卷 742-746页

作者：谭洪贺孙义和清华大学微电子学研究所北京100084

基于一款通用的16位定点数字信号处理器，结合D／A转换器、A／D转换器和放大器等模拟电路模块，设计并实现了一种面向音频应用的可配置片上系统。该系统支持立体声输入输出，具有8～48kHz之间可编程的采样频率，以及可编程的输入输出放... 详细信息

基于一款通用的16位定点数字信号处理器，结合D／A转换器、A／D转换器和放大器等模拟电路模块，设计并实现了一种面向音频应用的可配置片上系统。该系统支持立体声输入输出，具有8～48kHz之间可编程的采样频率，以及可编程的输入输出放大器增益。同时，设计使用了24位高精度2-AA／D转换器，并配有可供选择的数字滤波器。为支持不同应用，系统提供24位或16位的可编程字长调节。系统芯片工作在1．8V电压下，芯片内各部分支持挂起或睡眠状态，有利于低功耗的便携式应用开发。介绍了部分关键功能模块的仿真、验证和测试，以及整个系统仿真模型的建立。

关键词：数字信号处理器片上系统 D/A转换器 A/D转换器

基于模糊综合评价法的第三方物流企业服务质量评价分析研究

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物流科技 2024年第10期47卷 99-105页

作者：孙飞燕安琦红河学院云南蒙自661100

消费者对服务要求的不断提升及激烈的行业竞争都对第三方物流企业服务质量提出了更高要求。文章结合SERVQUAL模型和第三方物流企业的实际,以有形性、可靠性、响应性、保证性、移情性作为一级指标,17个影响因素作为二级指标,首先邀请物... 详细信息

消费者对服务要求的不断提升及激烈的行业竞争都对第三方物流企业服务质量提出了更高要求。文章结合SERVQUAL模型和第三方物流企业的实际,以有形性、可靠性、响应性、保证性、移情性作为一级指标,17个影响因素作为二级指标,首先邀请物流行业专家进行打分,采用层次分析法构建判断矩阵得出影响因素的权重。其次选取云南省第三方物流企业的个人客户为调查对象,通过调查问卷获取其对各影响因素的评价,利用模糊综合评价法进行分析,得出评价结果靠后的影响因素是提供个性化关怀、设施设备、最优路线、员工素养、顾客和企业之间缺乏信任。因此,研究结果为第三方物流企业服务质量的不断改进提升给予一定的参考,为提升客户满意度给出了建议对策。

关键词：第三方物流企业物流服务质量服务质量评价层次分析法模糊综合评价法

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公共文化服务体系建设主体责任分析

牡丹江师范学院学报：社会科学版 2021年第6期 54-63页

作者：孙鸿鹤黑龙江省社会科学院文献数据中心黑龙江哈尔滨150028

政府是公共文化服务体系建设的主体。“十四五”时期,我国政府需更好地履行政府在公共服务体系建设中“建、投、管、导、督”地主体责任,更好地保障人民文化权益。

关键词：政府公共文化服务体系建设主体责任

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不同产地“瑞香红”苹果果实品质差异分析

中国南方果树 2024年第1期53卷 157-160页

作者：孙鲁龙高华梁俊赵政阳西北农林科技大学园艺学院陕西杨凌712100

为探讨“瑞香红”苹果的适宜栽培区域,从全国范围内选取了5个不同产地的盛果期果园采样,对果实的主要品质性状进行了差异分析。结果表明,不同产地的“瑞香红”果实品质差异较大,但各产地果实的果形指数无显著差异,均可达到1.02左右,均... 详细信息

为探讨“瑞香红”苹果的适宜栽培区域,从全国范围内选取了5个不同产地的盛果期果园采样,对果实的主要品质性状进行了差异分析。结果表明,不同产地的“瑞香红”果实品质差异较大,但各产地果实的果形指数无显著差异,均可达到1.02左右,均表现该品种的高桩特性。综合分析结果表明,陕西白水和云南红河两产地的“瑞香红”综合品质较好,主要表现为果个大,单果质量大,硬度适中,可溶性固形物含量及固酸比高;而山西临猗产的“瑞香红”果实综合品质较差,主要表现为果个小,单果质量小,酸度大,固酸比小。本研究可为相似条件下引种“瑞香红”提供参考。

关键词：苹果 “瑞香红” 产地品质

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铝合金搅拌摩擦焊接头的组织缺陷与工艺参数关系的研究

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热加工工艺 2018年第13期47卷 45-47,52页

作者：孙金睿朱海于洪河关平孙龙东北林业大学机电工程学院黑龙江哈尔滨150040 通钢集团吉林市焊管有限责任公司吉林吉林132005

通过改变搅拌头下压量、转速、焊接速度对3 mm厚6061铝合金板进行了搅拌摩擦焊试验,对接头的组织和力学性能进行了检测分析。结果表明,较小的下压量会造成未焊透缺陷和弱结合缺陷。焊接热输入过高时,材料的流动性过高,采用无螺纹的搅拌... 详细信息

通过改变搅拌头下压量、转速、焊接速度对3 mm厚6061铝合金板进行了搅拌摩擦焊试验,对接头的组织和力学性能进行了检测分析。结果表明,较小的下压量会造成未焊透缺陷和弱结合缺陷。焊接热输入过高时,材料的流动性过高,采用无螺纹的搅拌针无法正常挤压推动其填充空腔,形成孔洞缺陷;焊接热输入过低时,也会造成孔洞缺陷。要合理匹配搅拌头转速和焊接速度,焊接速度低时,转速也应较低,反之,焊接速度高时,必须相应提高转速,才能有效抑制孔洞缺陷。

关键词：搅拌摩擦焊孔洞缺陷热输入转速焊接速度

Delineating the distinct roles of PD-L1 alternative splicing isoforms in colorectal cancer

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Delineating the distinct roles of PD-L1 alternative splicing...