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Journal of Integrative Plant Biology 2016年第9期58卷 762-765页

作者： Gaojie Ji Jie Zhang Haiying Zhang honghe sun Guoyi Gong Jianting Shi Shouwei Tian Shaogui Guo Yi Ren Huolin Shen Junping Gao Yong Xu Beijing Vegetable Research Center National Engineering Research Center for Vegetables Beijing Academy of Agriculture and Forestry Sciences Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China)Ministry of Agriculture Beijing Key Laboratory of Vegetable Germplasm ImprovementBeijing 100097China Department of Vegetable Science College of Horticulture China Agricultural University Beijing 100097 China

Summary Although it has been reported previously that ethylene plays a critical role in sex determination in cucurbit species, how the andromonoecy that carries both the male and hermaphroditic flowers is determined in watermelon is still unknown. Here we showed that the watermelon gene 1-aminocyclopropane-1-carboxylate syn- thase 4 CCitACS4）, expressed specifically in carpel primor- dia, determines the andromonoecy in watermelon. Among four single nucleotide polymorphism （SNPs） and one lnDel identified in the coding region of CitACS4, the C364W mutation located in the conserved box 6 was co- segregated with andromonoecy. Enzymatic analyses showed that the C364W mutation caused a reduced activity in CitACS4. We believe that the reduced CitACS4 activity may hamper the programmed cell death in stamen primordia, leading to the formation of hermaphroditic flowers.

关键词： 1-aminocyclopropane-1-carboxylic acid synthase Citrullus lanatus sex determination

A Genomic Variation Map Provides Insights into the Genetic Basis of Spring Chinese Cabbage (Brassica rapa ***)Selection

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Molecular Plant 2018年第11期11卷 1360-1376页

作者： Tongbing Su Weihong Wang Peirong Li Bin Zhang Pan Li Xiaoyun Xin honghe sun Yangjun Yu Deshuang Zhang Xiuyun Zhao Changlong Wen Gang Zhou Yuntong Wang Hongkun Zheng Shuancang Yu Fenglan Zhang Beijing Vegetable Research Center (BVRC) Beijing Academy of Agriculture and Forestry Science (BAAFS)Beijing100097China Department of Crop Genetics John Innes CentreNorwich NR47UHUK Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China) Ministry of AgncultureBeijing 100097China Beijing Key Laboratory of Vegetable Germplasm Improvement Beijing 100097China Biomarker Technologies Corporation BeijingChina

Chinese cabbage is the most consumed leafy crop in East Asian ***,premature bolting induced by continuous low temperatures severely decreases the yield and quality of the Chinese cabbage, and therefore restricts its planting season and geographic *** the past 40years,spring Chinese cabbage with strong winterness has been selected to meet the market ***,we report a genome variation map of Chinese cabbage generated from the resequencing data of 194 geographically diverse accessions of three ***-depth analyses of the selection sweeps and genome-wide patterns revealed that spring Chinese cabbage was selected from a specific population of autumn Chinese cabbage around the area of Shandong peninsula in northern *** identified 23 genomic loci that underwent intensive selection,and further demonstrated by gene expression and haplotype analyses that the incorporation of elite alleles of VERNALISATION INSENTIVE 3.1(BrVIN3.1)and FLOWER LOCUS C 1(BrFLC1)is a determinant genetic source of variation during ***,we showed that the quantitative response of BrVIN3.1 to cold due to the sequence variations in the cis elements of the BrVlN3.1 promoter significantly contributes to bolting-time variation in Chinese ***, our study provides valuable insights into the genetic basis of spring Chinese cabbage selection and will facilitate the breeding of bolting-resistant Varieties by molecular-marker-assisted selection,transgenic or gene editingapproaches.

关键词： Chinese-cabbage selection vernalization flowering time VERNALISA TION INSENTIVE 3 FLOWER LOCUS C 1

A key‘foxy’aroma gene is regulated by homologyinduced promoter indels in the iconic juice grape‘Concord’

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Horticulture Research 2020年第1期7卷 2028-2039页

作者： Yingzhen Yang JoséCuenca Nian Wang Zhenchang Liang honghe sun Benjamin Gutierrez Xiaojun Xi Jie Arro YiWang Peige Fan Jason Londo Peter Cousins Shaohua Li Zhangjun Fei Gan-Yuan Zhong US Department of Agriculture-Agricultural Research Service Grape Genetics Research UnitGenevaNYUSA Beijing Key Laboratory of Grape Sciences and Enology Laboratory of Plant ResourcesInstitute of BotanyChinese Academy of SciencesBeijingChina Boyce Thompson Institute for Plant Research Cornell UniversityIthacaNYUSA US Department of Agriculture-Agricultural Research Service Plant Genetic Resources UnitGenevaNYUSA Forestry and Pomology Research Institute Shanghai Academy of Agricultural SciencesShanghaiChina University of Chinese Academy of Sciences BeijingChina E.&J.Gallo Winery ModestoCAUSA US Department of Agriculture–Agricultural Research Service Robert W.Holley Center for Agriculture and HealthIthacaNYUSA Present address:Centro de Citricultura y Producción Vegetal.Instituto Valenciano de Investigaciones Agrarias MoncadaValenciaSpain Present address:College of Horticulture and Forestry Huazhong Agricultural UniversityWuhanHubeiChina

‘Concord’,the most well-known juice grape with a parentage of the North American grape species Vitis labrusca L.,possesses a special‘foxy’aroma predominantly resulted from the accumulation of methyl anthranilate(MA)in *** aroma,however,is often perceived as an undesirable attribute by wine consumers and rarely noticeable in the common table and wine grape species *** we discovered homology-induced promoter indels as a major genetic mechanism for species-specific regulation of a key‘foxy’aroma gene,anthraniloyl-CoA:methanol acyltransferase(AMAT),that is responsible for MA *** found the absence of a 426-bp and/or a 42-bp sequence in AMAT promoters highly associated with high levels of AMAT expression and MA accumulation in‘Concord’and other ***-derived *** promoter variants,all with direct and inverted repeats,were further confirmed in more than 1,300 Vitis ***,functional impact of these indels was validated in transgenic *** on the promoter regulation,large structural changes including exonic insertion of a retrotransposon were present at the AMAT locus in some *** *** of the AMAT genetic regulation advances our understanding of the‘foxy’aroma trait and makes it genetically trackable and amenable in grapevine breeding.

关键词： insertion aroma grape

Devonian integrative stratigraphy,biotas,and paleogeographical evolution of the Qinghai-Tibetan Plateau and its surrounding areas

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Science China Earth Sciences 2024年第4期67卷 1036-1070页

作者： Wenkun QIE Kun LIANG Wen GUO Biao GAO Junjun SONG Bo CHEN Pu HUANG Li QIAO honghe XU Jitao CHEN Yucong sun Yichun ZHANG State Key Laboratory of Palaeobiology and Stratigraphy Nanjing Institute of Geology and PalaeontologyChinese Academy of SciencesNanjing 210008China University of Chinese Academy of Sciences Nanjing 211135China

The Devonian System in the Qinghai-Tibetan Plateau and its surrounding areas is widely distributed,diverse in lithotypes and rich in *** records the crucial processes of continuous subduction and reduction of the Paleo-Asian Ocean in the northern hemisphere and the transformation of the Proto-Tethys Ocean into the Paleo-Tethys Ocean in the southern ***,it is of great significance for explaining the global paleogeographic evolution,tectonic activities and biodiversity changes during this critical *** blocks on both sides of the southern Tienshan suture zone and the Longmu Co-ShuanghuChangning-Menglian suture zone belong to different sedimentary systems and paleobiogeographic realms and regions,showing important tectonic paleogeographic partitioning *** two suture zones represent the main branches of the PaleoAsian Ocean and the Paleo-Tethys Ocean,respectively,and on this basis the Devonian System can be subdivided into three types:the Tienshan type,the Tethys type and the Gondwana *** on recent research progress on the Devonian stratigraphy and paleontology in combining provenance analysis and biotic characteristics in the Qinghai-Tibetan Plateau and surrounding areas,this paper aims to establish the latest integrative stratigraphic framework and conduct paleogeographic reconstruction,providing foundation for exploring the geological evolution and dynamic mechanisms of various terranes and blocks in the northern margin of *** results indicate that the North Qiangtang,South Qiangtang,Simao,Baoshan and Tengchong terranes,as well as the Himalaya Tethys Zone and the South China Block have affinities with the Indian Block,while the Lhasa Terrane has affinities with the Australian Block,and may be located between the South China and Australian *** the Emsian(Early Devonian),the South China Block separated from the northern margin of Gondwana,a process that was accompanied by the opening of the Jinshajiang-Ailaosh

关键词： Devonian Paleo-Tethys Ocean Integrative stratigraphy Biotas Provenance Analysis Paleogeography

Karyotype Stability and Unbiased Fractionation in the Paleo-Allotetraploid Cucurbita Genomes

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Molecular Plant 2017年第10期10卷 1293-1306页

作者： honghe sun Shan WU Guoyu Zhang Chen Jiao Shaogui Guo Yi Ren Jie Zhang Haiying Zhang Guoyi Gong Zhangcai Jia Fan Zhang Jiaxing Tian William J. Lucas Jeff J. Doyle Haizhen Li Zhangjun Fei Yong Xu National Engineering Research Center for Vegetables Beijing Academy of Agriculture and Forestry Sciences Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China) Beijing Key Laboratory of Vegetable Germplasm Improvement Beijing 100097 China Boyce Thompson Institute Cornell University Ithaca NY 14853 USA Department of Plant Biology College of Biological Sciences University of California Davis CA 95616 USA Section of Plant Breeding & Genetics School of Integrated Plant Sciences Cornell University Ithaca NY 14853 USA USDA-ARS Robert W. Holley Center for Agriculture and Health Ithaca NY 14853 USA

The Cucurbita genus contains several economically important species in the Cucurbitaceae family. Here, we report high-quality genome sequences of C. maxima and C. moschata and provide evidence supporting an allotetraploidization event in Cucurbita. We are able to partition the genome into two homoeologous subgenomes based on different genetic distances to melon, cucumber, and watermelon in the Benincaseae tribe. We estimate that the two diploid progenitors successively diverged from Benincaseae around 31 and 26 million years ago （Mya）, respectively, and the allotetraploidization happened at some point between 26 Mya and 3 Mya, the estimated date when C. maxima and C. moschata diverged. The subgenomes have largely maintained the chromosome structures of their diploid progenitors. Such long-term karyotype stability after polyploidization has not been commonly observed in plant polyploids. The two subgenomes have retained similar numbers of genes, and neither subgenome is globally dominant in gene expression. Allele-specific expression analysis in the C. maxima ×C. moschata interspecific F1 hybrid and their two parents indicates the predominance of trans-regulatory effects underlying expression divergence of the parents, and detects transgressive gene expression changes in the hybrid correlated with heterosis in important agronomic traits. Our study provides insights into polyploid genome evolution and valuable resources for genetic improvement of cucurbit crops.

关键词： Cucurbita maxima Cucurbita moschata paleo-allotetraploid karyotype stability unbiased fractionation allele-specific expression

iTAK： A Program for Genome-wide Prediction and Classification of Plant Transcription Factors, Transcriptional Regulators, and Protein Kinases

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Molecular Plant 2016年第12期9卷 1667-1670页

作者： Yi Zheng Chen Jiao honghe sun Hernan G. Rosli Marina A. Pombo Peifen Zhang Michael Banf Xinbin Dai Gregory B. Martin James J. Giovannoni Patrick X. Zhao Seung Y. Rhee Zhangjun Fei USDA-ARS Robert W. Holley Center for Agriculture and Health Ithaca NY 14853 USA

Dear Editor,Transcription factors （TFs） are proteins that regulate the expression of target genes by binding to specific cis-elements in pro-moter regions. Transcriptional regulators （TRs） a）so regulate the expression of target genes; however, they operate indirectly via interaction with the basal transcription apparatus （e.g., TFs）, or by altering the accessibility of DNA to TFs via chromatin remodeling. Another type of regulatory proteins, protein kinases （PKs）,

关键词： Mystery Mendel's Magnesium

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公共文化服务体系建设主体责任分析

牡丹江师范学院学报：社会科学版 2021年第6期 54-63页

作者：孙鸿鹤黑龙江省社会科学院文献数据中心黑龙江哈尔滨150028

政府是公共文化服务体系建设的主体。“十四五”时期,我国政府需更好地履行政府在公共服务体系建设中“建、投、管、导、督”地主体责任,更好地保障人民文化权益。

关键词：政府公共文化服务体系建设主体责任

苔藓样型皮肤淀粉样变的免疫组化和超微结构研究

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中华皮肤科杂志 1996年第1期29卷 55-56页

作者：汪晨张晶潘子昂孙洪河何静文中日友好医院皮肤科北京100029 中日友好医院病理科北京100029 中日友好医院临床医学研究所电镜室北京100029 中日友好医院药剂科北京100029 中日友好医院电教室北京100029

最近我们对9例苔藓样型皮肤淀粉样变(PLCA)采用免疫组化和电镜观察的方法对淀粉样蛋白的来源进行了研究。一、病例和方法9例中,男7例,女2例;年龄42～64岁,平均54.9岁;病期3～40年,平均17.9年。分别于其典型皮疹处取材,10%甲醛液中固定,... 详细信息

最近我们对9例苔藓样型皮肤淀粉样变(PLCA)采用免疫组化和电镜观察的方法对淀粉样蛋白的来源进行了研究。一、病例和方法9例中,男7例,女2例;年龄42～64岁,平均54.9岁;病期3～40年,平均17.9年。分别于其典型皮疹处取材,10%甲醛液中固定,石蜡包埋,HE。

关键词： PLCA 皮肤淀粉样变免疫组织化学超微结构

Efficient homojunction tin perovskite solar cells enabled by gradient germanium doping

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Efficient homojunction tin perovskite solar cells enabled by...

1例不明原因多器官受损患者中毒原因多策略质谱技术确证

第十一届新型太阳能材料科学与技术学术研讨会

作者： Zhenzhu Zhao Mulin sun Yuyang Ji Kaitian Mao Zongming Huang Chengjian Yuan Yuqian Yang honghe Ding Yingguo Yang Yu Li Wenjing Chen Junfa Zhu Jing Wei Jixian Xu Watcharaphol Paritmongkol Antonio Abate Zhengguo Xiao Lixin He Qin Hu School of Microelectronics University of Science and Technology of China

P-type self-doping is known to hamper tin-based perovskites for developing highperformance solar cells by increasing background current density and carrier recombination *** this work,we propose a gradient homojunction structure with germanium doping that generates an internal electric field across the perovskite film to deplete the charge *** structure reduces the dark current density of perovskite by over two orders of magnitude and trap density by an order of *** resultant tin-based perovskite solar cells exhibit a higher power conversion efficiency of 13.2% and excellent stability,maintaining 95% and 85% of their initial efficiencies after 250 min of continuous illumination and 3800 h of storage,*** reveal the homojunction formation mechanism using density functional theory calculations and molecular-level *** work provides a reliable strategy for controlling the spatial energy levels in tin perovskite films and offers insights for designing intriguing lead-free perovskite optoelectronics.

关键词： tin-based perovskite gradient homojunction perovskite solar cells

来源： cnki会议评论

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中国热带医学 2024年第5期24卷 612-617页

作者：赵凌国甄珍杨莹雷蕾涂云亮马浩嘉刘红河孙雯雯深圳市宝安区疾病预防控中心广东深圳518101 北京大学深圳医院广东深圳518036 深圳市疾病预防控制中心广东深圳518073 上海爱博才思分析仪器贸易有限公司上海200335

目的调查1例不明原因导致多器官受损的中毒的原因,确证致病因子种类和暴露量,查明致病因子来源,为患者临床救治以及此类事件预防提供参考和依据。方法从患者长期服用的不明中药入手,首先用靶向策略进行筛查,考察是否为生物碱中毒,然后... 详细信息

目的调查1例不明原因导致多器官受损的中毒的原因,确证致病因子种类和暴露量,查明致病因子来源,为患者临床救治以及此类事件预防提供参考和依据。方法从患者长期服用的不明中药入手,首先用靶向策略进行筛查,考察是否为生物碱中毒,然后采用非靶向策略信息依赖采集模式(IDA)筛查患者血液、尿液和药物,结合可疑化合物的毒理作用和患者临床表现,确定致病因子。根据标准品建立定量方法,对全部样品中致病因子进行定量检测。流行病学调查获得患者服药史,结合药物规格及含量检测结果,确定各致病因子暴露量。最后采用数据非依赖序列窗口采集模式(SWATH)筛查全部样品,无偏采集化合物二级质谱信息,对已确证的致病因子进行验证和补充。结果靶向筛查排除常见生物碱中毒。患者血液、尿液均筛查出他达拉非,不明中成药检出他达拉非、西地那非、他达拉非甲基氯化物、扑热息痛和双氯芬酸,上述化合物副作用与患者临床表现相符。他达拉非、西地那非、他达拉非甲基氯化物、双氯芬酸和扑热息痛的长期日均暴露剂量最高分别为30.5、15.8、0.05、45.6和3.2 mg,严重过量。序列窗口采集模式同样筛查出上述5种药物,并检出盐酸巴马汀。结论本研究综合采用靶向筛查、非靶向筛查和靶向定量策略、数据依赖和数据非依赖采集模式,结合患者临床表现与流行病学资料,确定本例是由长期过量服用添加化学成分的中成药引起的药物中毒。本研究可为临床不明原因中毒患者诊断和调查提供思路,为相关中毒事件的应急检测和处置提供参考。

关键词：中毒非靶向筛查靶向筛查质谱他达拉非