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减氮对麦玉轮作农田土壤反硝化细菌群落结构和多样性的影响

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农业环境科学学报 2024年第6期43卷 1338-1349页

作者：白芳芳乔冬梅李平杜臻杰陆红飞郭魏齐学斌中国农业科学院农田灌溉研究所河南新乡453002 农业农村部农产品质量安全水环境因子风险评估实验室河南新乡453002 中国农业科学院河南新乡农业水土环境野外科学观测试验站河南新乡453002 江苏农林职业技术学院江苏句容212400

探究减氮对华北地区麦玉轮作农田土壤反硝化细菌群落结构和多样性影响,为华北地区麦玉轮作农田氮肥管理提供技术支持,本研究以不施氮为对照(CK),设置2个施氮量,分别为常规施氮量(纯氮300 kg·hm^(-2),N2)、减氮20%(纯氮240 kg·hm^(-2),... 详细信息

探究减氮对华北地区麦玉轮作农田土壤反硝化细菌群落结构和多样性影响,为华北地区麦玉轮作农田氮肥管理提供技术支持,本研究以不施氮为对照(CK),设置2个施氮量,分别为常规施氮量(纯氮300 kg·hm^(-2),N2)、减氮20%(纯氮240 kg·hm^(-2),N1),提取土壤DNA,用nirS(细胞色素cd1-亚硝酸还原酶)、nirK(Cu-亚硝酸还原酶)引物扩增后采用MiSeq PE300测序技术,研究施氮量对麦玉轮作农田土壤性状及nirS、nirK反硝化细菌群落结构及多样性的影响。结果表明,3个处理nirS、nirK反硝化细菌α-多样性指数无显著差异,nirS反硝化细菌α-多样性高于nirK。减氮显著影响nirS、nirK反硝化细菌物种组成。减氮对nirS、nirK反硝化细菌门水平及nirK纲水平物种组成无显著影响,但显著降低了nirS反硝化细菌Deltaproteobacteria(δ-变形菌纲)相对丰度;减氮显著影响nirS、nirK属水平物种组成。硝态氮、速效磷、pH值是影响土壤nirS反硝化细菌属水平群落结构主要环境因子;pH值是影响土壤nirK反硝化细菌属水平群落结构的主要环境因子。研究表明,适量减氮不影响反硝化细菌α-多样性,但显著影响反硝化细菌属水平群落组成和群落结构,减氮主要是通过影响土壤性状及微生物群落结构进而影响农田土壤N2O排放。

关键词：减氮 nirK nirS 反硝化细菌麦玉轮作多样性群落结构

荧光探针PTAID固态试纸法测定土壤中Pd^(2+)和Cu^(2+)的比较研究

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环境监控与预警 2024年第2期16卷 53-58页

作者：董铮王琳田芳陆鸿飞江苏省镇江环境监测中心江苏省镇江土壤特色实验室江苏镇江212000 江苏科技大学江苏镇江212003

将新型荧光探针PTAID以固态形式用于检测土壤中的钯离子(Pd^(2+))和铜离子(Cu^(2+)),检出限分别为0.63和1.19μmol/L,说明探针PTAID对Pd^(2+)和Cu^(2+)有很高的灵敏度。通过条件优化实验,说明探针PTAID在pH值为4.0~12.0,反应时间为0~15 ... 详细信息

将新型荧光探针PTAID以固态形式用于检测土壤中的钯离子(Pd^(2+))和铜离子(Cu^(2+)),检出限分别为0.63和1.19μmol/L,说明探针PTAID对Pd^(2+)和Cu^(2+)有很高的灵敏度。通过条件优化实验,说明探针PTAID在pH值为4.0~12.0,反应时间为0~15 min内可以快速检测Pd^(2+)和Cu^(2+)。对土壤样品中的Pd^(2+)和Cu^(2+)进行检测,该方法与电感耦合等离子体发射光谱法相比,准确度和精密度均较高,具有响应时间短、灵敏度高、选择性好等优点,在土壤重金属检测方面具有广阔的应用前景。

关键词：荧光探针PTAID 土壤重金属钯离子铜离子电感耦合等离子体发射光谱

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基于磁梯度张量的滑坡监测方法

传感器与微系统 2024年第10期43卷 142-145页

作者：卢鸿飞王宇澄高迪驹张松勇渐开旺上海海事大学航运技术与控制工程交通运输行业重点实验室上海201306

滑坡监测是滑坡防治的有效技术手段,针对钻孔测斜仪在滑坡深部发生大位移时失效的问题,提出一种基于磁梯度张量的滑坡监测方法。该方法以磁梯度张量理论为基础,使用了十字形阵列进行监测,通过测得的磁感应强度三分量得到轴心的梯度值,... 详细信息

滑坡监测是滑坡防治的有效技术手段,针对钻孔测斜仪在滑坡深部发生大位移时失效的问题,提出一种基于磁梯度张量的滑坡监测方法。该方法以磁梯度张量理论为基础,使用了十字形阵列进行监测,通过测得的磁感应强度三分量得到轴心的梯度值,再根据反演计算出目标磁源的空间坐标,通过实验验证了该方法的可行性。在测量前将磁传感器阵列固定并计算偏离角,之后使永磁体按照矩形轨迹缓慢运动,并选取一定的测量点验证了该方法的精度,三轴平均绝对误差在0.1m以内,三轴最大误差在0.2m以内。最后分析了该方法存在误差的原因。

关键词：滑坡磁梯度张量磁传感器磁定位

基于荧光共轭聚合物和氧化石墨烯的核酸外切酶I检测

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分析试验室 2024年第4期43卷 541-546页

作者：程昆淇高梦莹邢佳贝赵一帆卢宏飞刘学国邢小静南阳师范学院化学与制药工程学院南阳473061 南阳理工学院生物与化学工程学院南阳473004

核酸外切酶I(Exo I)作为一种重要的核酶参与了许多生物学过程,具有维持遗传信息稳定性的功能,因此建立有效检测Exo I活性的方法具有重要的研究价值。基于聚芴类荧光共轭聚合物-poly[(9,9-bis(6′-N,N,N-trimethyl-ammonium)hexyl)-fluor... 详细信息

核酸外切酶I(Exo I)作为一种重要的核酶参与了许多生物学过程,具有维持遗传信息稳定性的功能,因此建立有效检测Exo I活性的方法具有重要的研究价值。基于聚芴类荧光共轭聚合物-poly[(9,9-bis(6′-N,N,N-trimethyl-ammonium)hexyl)-fluorenylene phenylene dibromide](PFP)不能与吸附在氧化石墨烯(GO)表面的单链DNA结合的实验发现,借助GO对单链DNA和双链DNA作用力强度大小的差异,构建了一个背景信号低、灵敏度高的Exo I检测新方法。GO和PFP的最适浓度分别为2.5和7.2 mg/L。在最佳条件下,Exo I浓度在0.5~4.0 U/mL范围内与体系的荧光强度呈良好的线性关系,线性方程为F=-0.11c+0.861,检出限为0.16 U/mL。该方法可用于血清模拟样品中Exo I活性的检测,加标回收率为103.6%~106.0%。

关键词：荧光共轭聚合物氧化石墨烯 DNA 低背景核酸外切酶I

减氮对华北地区麦玉轮作农田土壤N2O排放调控机理

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灌溉排水学报 2024年第5期43卷 95-104页

作者：白芳芳李平陆红飞李白玉齐学斌毛雯雯中国农业科学院农田灌溉研究所河南新乡453002 农业部农产品质量安全水环境因子风险评估实验室河南新乡453002 中国农业科学院河南新乡农业水土环境野外科学观测试验站河南新乡453002 江苏农林职业技术学院江苏句容212400 青岛工学院山东青岛266300 河南省地质局地质灾害防治中心郑州450012

【目的】探究减氮对华北地区麦玉轮作农田土壤N2O排放调控机制。【方法】冬小麦季和夏玉米季均以不施氮为对照(CK),设置2个施氮量,分别为常规施氮量(纯氮300 kg/hm2,N2)、减氮20%(纯氮240 kg/hm2,N1),研究施氮量对麦玉轮作农田土壤性质... 详细信息

【目的】探究减氮对华北地区麦玉轮作农田土壤N2O排放调控机制。【方法】冬小麦季和夏玉米季均以不施氮为对照(CK),设置2个施氮量,分别为常规施氮量(纯氮300 kg/hm2,N2)、减氮20%(纯氮240 kg/hm2,N1),研究施氮量对麦玉轮作农田土壤性质及N2O排放通量影响,基于逐步回归分析,研究减氮对麦玉轮作农田土壤N2O排放调控机制。【结果】(1)减氮有效降低了N2O排放通量,且夏玉米季N2O排放通量远高于冬小麦季,夏玉米季由施肥引起的N2O排放量较高。(2)冬小麦季N2O排放通量与环境因子逐步回归公式为:N2O排放通量=181.952+1.450×硝态氮+8.401×铵态氮-0.514×电导率;硝态氮、铵态氮会对冬小麦季N2O排放通量产生显著的正向影响,而电导率会对冬小麦季N2O排放通量产生显著的负向影响。(3)夏玉米季N2O排放通量与环境因子逐步回归公式为:N2O排放通量=-354.606+4.592×硝态氮+157.848×铵态氮;硝态氮、铵态氮会对夏玉米季N2O排放通量产生显著的正向影响。【结论】综上可知,适量减氮显著降低夏玉米季N2O累积排放量和增温潜势,应加强夏玉米季农田水肥管理。

关键词：麦玉轮作减氮 N2O排放通量土壤性质逐步回归分析

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Highly efficient perovskite solar cells based on symmetric hole transport material constructed with indaceno[1,2-b:5,6-b’]dithiophene core building block

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Journal of Energy Chemistry 2020年第4期29卷 98-103页

作者： Cheng Wu Cheng Chen Li Tao Xingdong Ding Mengmeng Zheng Hongping Li Gongqiang Li hongfei lu Ming Cheng Institute for Energy Research Key Laboratory of ZhenjiangJiangsu UniversityZhenjiang 212013JiangsuChina School of Environmental and Chemical Engineering Jiangsu University of Science and TechnologyZhenjiang 212003JiangsuChina Key Laboratory of Flexible Electronic(KLOFE)&Institute of Advanced Materials(IAM) Nanjing Tech University(NanjingTech)Nanjing 211816JiangsuChina

Two novel hole transport materials (HTMs) with indaceno[1,2-b:5,6-b’]dithiophene (IDT) as core building blocks,termed IDT1 and IDT2,were designed and *** side alkyl chains were introduced to regulate and control the morphology and stacking behavior of HTMs,and the peripheral triarylamine arms were introduced to adjust the energy levels and to facilitate efficient hole *** in mesoporous structured perovskite solar cells (PSCs),HTM IDT1 achieved higher power conversion efficiency (PCE,19.55%) and better stability than Spiro-OMeTAD (19.25%) and IDT2 (15.77%) based *** results suggest the potential of IDTl as a promising HTM for PSCs.

关键词： Perovskite solar cell Hole transport material Mesoporous Indaceno[1,2-b,6-b’]dithiophene

Homogenous lithium plating/stripping regulation by a mass-producible Zn particles modified Li-metal composite anode

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Nano Research 2021年第11期14卷 3999-4005页

作者： ZiLi Zhang Yang Jin Yu Zhao Jing Xu Bin Sun Kai Liu hongfei lu Nawei Lv Zhimin Dang Hui Wu School of Electrical Engineering Zhengzhou UniversityZhengzhou450001China State key Laboratory of Power System Department of Electrical EngineeringTsinghua UniversityBeijing100084China State Key Lab of New Ceramics and Fine Processing School of Materials Science and EngineeringTsinghua UniversityBeijing100084China

A stable lithium-metal anode is critical for high performance lithium-metal batteries. However, heterogeneous Li plating/stripping may induce lithium dendrites formation on bare lithium-metal anode, which lowers the cell Coulombic efficiency and weakens battery safety. We found that bare Li metal surface becomes bumpy and cratered with numerous pits formation during Li stripping. These pits enhance electric field distortion and heterogeneous ion distribution during plating. Li plating preferentially happens on the edge of the pits, intensifying the voltage variation and Li dendrites growth, which leads to the cell rapid death or separator piercing. Herein, we propose a facile and mass-producible method to homogenize Li plating/stripping via adding lithiophilic particles into Li metal. Zinc particles were uniformly pressed in Li metal by a facile and scalable physical strategy of “rolling”, and transformed into LiZn alloy in situ through Li-Zn alloying at room temperature in a few minutes. The critical role of modified LiZn/Li composite anode in stabilizing electrode surface was revealed by both electrochemical test and simulation. Compared with bare Li anode, the evenly dispersed LiZn alloy particles in Li metal can effectively regulate the Li plating/stripping on electrode surface, reducing deepness of pits during stripping and directionally inducing Li plating to maintain electrode surface stability. On this basis, the pits depth of LiZn/Li composite during Li stripping is reduced to ∼ 15 μm, which is much shallower than that of bare Li metal of ∼ 40 μm. The LiZn/Li composite electrode can stably cycle for 600 h under Li plating/stripping capacity of 1 mAh·cm−2 and current density of 1 mA·cm−2 without any short circuit. Furthermore, assembled LiZn/Li||LiFePO4 full cell presents better cycling stability and rate performances than that of based on bare Li anode.

关键词： lithium metal batteries Li-Zn allloy Li-metal composite anode regulation of current distribution homogenization Li plating/stripping

维奈克拉为基础联合方案治疗慢性粒细胞白血病急变及髓系肉瘤2例并文献复习

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白血病．淋巴瘤 2024年第4期33卷 235-239页

作者：吴珍珍张茂功陆鸿飞郭影李变贞张晶晶姚莉红河南科技大学附属三门峡黄河医院血液科三门峡472000

目的探索维奈克拉为基础联合方案治疗慢性粒细胞白血病(CML)急变及髓系肉瘤的效果。方法回顾性分析河南科技大学附属三门峡黄河医院诊治的1例CML急变及1例髓系肉瘤患者的临床资料,并复习相关文献。结果CML患者急变期及造血干细胞移植后... 详细信息

目的探索维奈克拉为基础联合方案治疗慢性粒细胞白血病(CML)急变及髓系肉瘤的效果。方法回顾性分析河南科技大学附属三门峡黄河医院诊治的1例CML急变及1例髓系肉瘤患者的临床资料,并复习相关文献。结果CML患者急变期及造血干细胞移植后复发先后应用维奈克拉联合普纳替尼及维奈克拉联合达沙替尼诱导治疗1个周期,均达完全缓解骨髓象。髓系肉瘤患者用维奈克拉联合低剂量阿糖胞苷及维奈克拉联合去甲基化药物治疗2个周期症状消失,增强CT提示部分缓解。结论维奈克拉为基础联合方案治疗CML急变及移植后复发、髓系肉瘤的近期效果良好,可能作为体能状态较差患者强化疗或其他治疗的桥接治疗选择。

关键词：肉瘤,髓样慢性粒细胞白血病维奈克拉药物疗法,联合

Effects of Bacillus subtilis and Saccharomyces cerevisiae inoculation on soil bacterial community and rice yield under combined irrigation with reclaimed and fresh water

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International Journal of Agricultural and Biological Engineering 2022年第3期15卷 33-46页

作者： hongfei lu Xuebin Qi Ping Li Dongmei Qiao Shafeeq-ur-Rahman Fangfang Bai Jiaxin Cui Farmland Irrigation Research Institute Chinese Academy of Agricultural SciencesXinxiang 453003HenanChina Water Environment Factor Risk Assessment Laboratory of Agricultural Products Quality and Safety Ministry of Agriculture and Rural AffairsXinxiang 453002HenanChina Agricultural Water Soil Environmental Field Research Station of Xinxiang Chinese Academy of Agricultural SciencesXinxiang 453002HenanChina Jiangsu Vocational College of Agriculture and Forestry Jurong 212499JiangsuChina

In order to save fresh water and reduce soil salt accumulation,reclaimed water-fresh water combined irrigation,i.e,irrigation with reclaimed water for 50 d and then with fresh water till harvest,was used in rice *** subtilis and Saccharomyces cerevisiae were inoculated into the soil at the end of reclaimed water *** inoculation weight per pot of these microorganisms was as follows:0 g and 0 g(J0),5 g and 0 g(J1),3.75 g and 1.25 g(J2),2.5 g and 2.5 g(J3),1.25 g and 3.75 g(J4),and 0 g and 5 g(J5),*** using reclaimed water in the whole stage was used as the control(CK).The plant height,tiller,physical and chemical properties of the soil,and soil bacterial diversity were *** was found that the plant height of rice was increased significantly by J1-J5 *** dry weight of rice root,stem,and panicle and the 1000-grain weight increased significantly,while the leaf dry weight *** inoculation significantly increased the nutrient absorption capacity of the crops.J1,J2,and J4 treatments significantly increased the amount of nitrate-nitrogen,ammonium nitrogen,available phosphorus,and available potassium,while J3,J4,and J5 treatments increased the soil organic matter,and microbial inoculation significantly decreased the EC of soil.J4 treatment induced the largest reduction in EC,and microorganisms treatments increased soil *** function prediction based on the KEGG(Kyoto Encyclopedia of Genes and Genomes)pathway indicated that soil metabolic function was not significantly disturbed by the *** matter and pH are the two main factors affecting the structure of the bacterial community in soil.3.75 g of *** and 1.25 g of *** per pot is the best inoculation ratio.

关键词： Bacillus subtilis Saccharomyces cerevisiae rice reclaimed water soil