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Characterization of liquid–liquid phase separation using super-resolution and single-molecule imaging

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Biophysics Reports 2022年第1期8卷 2-13页

作者： hongchen zhang Shipeng Shao Yujie Sun State Key Laboratory of Membrane Biology & Biomedical Pioneering Innovation Center (BIOPIC) & School of Future Technology Peking University School of Life Sciences Peking University Beijing Institute of Heart Lung and Blood Vessel Disease Beijing Anzhen Hospital Capital Medical University

Liquid–liquid phase separation(LLPS) is an emerging phenomenon involved in various biological processes. The formation of phase-separated condensates is crucial for many intrinsically disordered proteins to fulfill their biological functions. Using the recombinant protein to reconstitute the formation of condensates in vitro has become the standard method to investigate the behavior and function of ***, there is an urgent need to characterize the LLPS in living cells. Importantly, condensates formed through LLPS at physical relevant concentrations are often smaller than the optical diffraction limit, which makes precise characterization and quantification inaccurate due to the scatter of *** booming development of super-resolution optical microscopy enables the visualization of multiple obscured subcellular components and processes, which is also suitable for the LLPS research. In this protocol, we provide step-by-step instructions to help users take advantage of super-resolution imaging to depict the morphology and quantify the molecule number of endogenous condensates in living cells using RNA Pol II as an example. This streamlined workflow offers exceptional robustness, sensitivity, and precision, which could be easily implemented in any laboratory with an inverted total internal reflection microscope. We expect that super-resolution microscopy will contribute to the investigation of both large and tiny condensates under physiological and pathological conditions and lead our understanding of the mechanism of LLPS to a higher and deeper layer.

关键词： Liquid–liquid phase separation Super-resolution imaging Single-molecule imaging

Are mindfulness and self-efficacy related to presenteeism among primary medical staff: A cross-sectional study

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International Journal of Nursing Sciences 2019年第2期6卷 182-186页

作者： Nan Tang Lin Han Purui Yang Yanhui Zhao hongchen zhang Lanzhou University LanzhouChina

Objectives: In ensuring public welfare with primary medical and health services,the primary medical staff faces new *** workload,and therefore degrees of stress and burnout,can influence job satisfaction and lead to presenteeism,which is defined as the appearance to be on the job but not actually *** purpose of this study is to investigate the current worling situation and the relationship between presenteeism and mindfulness of primary medical staff and determine the mediating effect of self-efficacy on this ***: A cross-sectional survey was performed with 580 primary medical staff from 9 hospitals in Shaanxi province,northwest ***,mindfulness,and self-efficacy were measured by using a general information questionnaire,the Five-Facet Mindfulness Questionnaire,the General Self-Efficacy Scale,and the Stanford Presenteeism *** effect was analyzed by a series of hierarchical multiple ***: A high level of presenteeism was found among 47.4% of the study *** was negatively correlated with mindfulness(r--0.409,P 0.05),the association was insignificant between presenteeism and ***: The results identified the effect of mindfulness on presenteeism of primary medical staff is realized through self-efficacy,which also suggested to enhance self-efficacy on center location when developing management strategies for mental health education or training among primary medical staff.

关键词： Medical staff,Hospital Mindfulness Presenteeism Self-efficacy

A pH-responsive biomimetic drug delivery nanosystem for targeted chemo-photothermal therapy of tumors

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Nano Research 2022年第5期15卷 4274-4284页

作者： Yanmin Ju Zhiyi Wang Zeeshan Ali hongchen zhang Yazhou Wang Nuo Xu Hui Yin Fugeng Sheng Yanglong Hou Beijing Key Laboratory for Magnetoelectric Materials and Devices(BKL-MMD) Beijing Innovation Centre for Engineering Science and Advanced Technology(BIC-ESAT)School of Materials Science and EngineeringPeking UniversityBeijing 100871China College of Pharmacy China Pharmaceutical UniversityNanjing 211198China Synthetic and Functional Biomolecules Center Department of Chemical BiologyCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China Fifth Medical Center of Chinese PLA General Hospital Beijing 100071China

Smart drug delivery nanosystem is significant for tumor treatments due to its possibility of temporally,spatially,and dose-controlled ***,the therapeutic efficacy of drug delivery nanosystem is often compromised in cancer treatment as the enrichment of therapeutic agents in the reticuloendothelial ***,doxorubicin(DOX)loaded biomimetic drug delivery nanosystem with macrophage cell membrane(MCM)camouflaged,MnFe_(2)O_(4)-DOX-MCM nanocube(NC),is developed for cancer treatment with tumor targeting,pH-stimuli drug release,and chemo-photothermal therapeutic *** nanosystem shows the capability of immune escape and enhanced cellular uptake of cancer cells due to the MCM ***-labile bond between the MnFe2O4 NCs and DOX remains stable at physiological condition and release drugs immediately in response to the endo-/lysosome pH ***,the photothermal effect of the nanosystem destroys tumor tissue,which further promotes chemotherapeutic *** vivo results demonstrate the tumor homing ability and produce a notable synergistic therapeutic effect of the ***,biomimetic pH-responsive drug delivery nanosystem,MnFe_(2)O_(4)-DOX-MCM NCs,is an effective nanoplatform,which might be potential application for cancer synergistic treatment.

关键词： drug delivery macrophage cell membrane pH stimuli synergistic therapy

Impairment of Autophagic Flux After Hypobaric Hypoxia Potentiates Oxidative Stress and Cognitive Function Disturbances in Mice

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Neuroscience Bulletin 2024年第1期40卷 35-49页

作者： Shuhui Dai Yuan Feng Chuanhao Lu hongchen zhang Wenke Ma Wenyu Xie Xiuquan Wu Peng Luo Lei zhang Fei Fei Zhou Fei Xia Li Department of Neurosurgery Xijing HospitalFourth Military Medical UniversityXi'an 710000China National Translational Science Center for Molecular Medicine and Department of Cell Biology Fourth Military Medical UniversityXi'an 710000China Department of Neurosurgery The Central Hospital of BaojiBaoji 721000China Department of Ophthalmology Xijing HospitalFourth Military Medical UniversityXi'an 710000China

Acute hypobaric hypoxic brain damage is a potentially fatal high-altitude *** plays a critical role in ischemic brain injury,but its role in hypo-baric hypoxia(HH)remains *** we used an HH chamber to demonstrate that acute HH exposure impairs autophagic activity in both the early and late stages of the mouse brain,and is partially responsible for HH-induced oxidative stress,neuronal loss,and brain *** autophagic agonist rapamycin only promotes the initiation of *** proteome analysis,a screen showed that protein dynamin2(DNM2)potentially regulates autophagic *** of DNM2 significantly increased the formation of autolysosomes,thus maintaining autophagic flux in combination with ***,the enhancement of autophagic activity attenuated oxidative stress and neurological deficits after HH *** results contribute to evidence supporting the conclusion that DNM2-mediated autophagic flux represents a new therapeutic target in HH-induced brain damage.

关键词： Autophagy-Proteomics Oxidative stress Hypobaric hypoxia-Brain injury

Research on Countermeasures and Development Status of Red Tourism in Shandong

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International Journal of Technology Management 2013年第8期 107-110页

作者： Liu hongchen zhang Lijie Shandong University (Weihai) Weihai Shandong 264200

As a new tourism pattern, red tourism develops rapidly in China. In Shandong, not only the red cultural heritage is rich, but also the red tourism resources are rich, but the development is not very successful now. The paper aims at surveying the status of Shandong red tourism, and analyzes the evaluation of tourists on red tourist attractions by the way of market research. The research indicates that red tourism has the problems including improper publicity, single development model and imperfect infrastructures, for which the paper proposes the suggestions such as multi-channel publicity and multi-mode development. The research results of the paper not only provides scientific basis for developing red tourism in Shandong, but also provides reference for practical management on red tourism.

关键词： Shandong red tourism development status market research SPSS analysis countermeasures

Characteristics of hydrocarbon source rocks of No.3 buried-hill region in Nanpu Sag

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Engineering Sciences 2014年第1期12卷 87-92,96页

作者： Ma Guanghua zhang hongchen Qian Fengyan Chen Haixia PetroChina Jidong Oilfield Company

Based on the data of 44 samples of hydrocarbon source rocks in Nanpu No.3 buffed-hill region, the kerogen type is judged through the pyrolysis and microscopic identification. At the same time, organic matter maturity and hydrocarbon generation threshold are studied by using vitrinite reflectance, pyrolysis yield and hydrocarbon abundance. Meanwhile the hydrocarbon expulsion threshold is calculated. And the characteristics of organic hydrocarbon generation and expulsion are preliminarily revealed and evaluated. The result shows that the No.3 buffed-hill region has abundant hydrocarbon source rocks with high content of organic carbon. And the primary types of kerogen are II, and lI 2. The hydrocarbon source rocks which passed biochemistry, thermolysis and thermal cracking have developed into the mature-postmature phase of different extents. And plenty of oil and gas were expelled out. It is believed the depth of oil-generating window is 3 600 m and the depth of hydro- carbon-expulsion threshold is 4 100 m. The comprehensive analysis indicates that Nanpu No.3 burried-hill region has a certain condition to generate hydrocarbon which is very promising in oil exploration and thus can become an important exploration and development target next.

关键词： hydrocarbon source rocks kerogen pyrolysis yield index oil threshold hydrocarbon-expulsion threshold

Shape description matrix and its applications to color-image retrieval and recognition

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Science China(Technological Sciences) 2004年第2期47卷 159-165页

作者： WANG Yi,ZHAI hongchen,LIANG Yanmei,zhang Siyuan & MU Guoguang Institute of Modern Optics,Nankai University,Tianjin 300071,China Key Laboratory of Information Sci-ence and Technology MOE,Tianjin 300071,China Institute of Modern Optics Nankai University Tianjin China Key Laboratory of Information Science and Technology MOE Tianjin China

Shape description matrix (SDM) describing regional pixel distribution characteristics of images is defined, with both radial and angular distribution messages taken into account to offer a comprehensive and exact description of the shape characteristics even upon a blurred edge. Using this SDM, a general color histogram (GCH) is developed. Both theoretical and experimental results show that the GCH can be applied to color-image retrievals and recognitions with a high efficiency.

关键词： SDM, similarity degree, match degree, general color histogram.

Nuclear actin promotes transcription factory formation through co-phase separation

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Nuclear actin promotes transcription factory formation throu...

2021年全国生物相分离和相变学术研讨会

作者： Shipeng Shao Mian Wei hongchen zhang Yujie Sun BIOPIC Peking University

Gene expression in response to external stimuli underlies a variety of fundamental cellular ***,how the transcription machinery is regulated under these scenarios is largely *** next-generation transcriptome sequencing and super-resolution microscopy,we discovered a novel role of nuclear actin in inducible transcriptional *** work unveiled nuclear actin in promoting the formation of transcription factory on inducible genes,acting as a general mechanism underlying the rapid response to environmental *** demonstrated that co-phase separation of RNA Pol Ⅱ,NWASP and nuclear actin plays an important role in enhancing transcription factory formation.

关键词： Nuclear actin Transcription factory Phase separation

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桑叶多酚对鲜切生菜的保鲜效果

食品与发酵工业 2022年第7期48卷 195-200页

作者：张宏琛余琳石慧西南大学食品科学学院重庆400715

桑叶是我国的药食两用农产品,而多酚被广泛认为是一种有较强抗氧化和抗菌能力的天然活性物质,但目前对桑叶多酚开发应用研究仍然较少。该文测定了5种桑叶多酚对大肠杆菌O157∶H7和单核增生李斯特菌的最小抑菌浓度(minimal inhibit conce... 详细信息

桑叶是我国的药食两用农产品,而多酚被广泛认为是一种有较强抗氧化和抗菌能力的天然活性物质,但目前对桑叶多酚开发应用研究仍然较少。该文测定了5种桑叶多酚对大肠杆菌O157∶H7和单核增生李斯特菌的最小抑菌浓度(minimal inhibit concentration,MIC),并选取效果最好的2个品种进行后续实验。为了研究桑叶多酚对鲜切生菜的保鲜效果,分析了鲜切生菜在室温(25℃)储藏4 d期间的品质变化。结果表明,相比于未处理组,MIC下(5 mg/mL)的桑叶多酚处理显著抑制了鲜切生菜中的叶绿素的降解和酚类物质的合成(P<0.05),显著降低了鲜切生菜在储藏期间的褐变度(P<0.05),且纳溪比嘉陵20效果好。同时,2种桑叶多酚显著抑制了鲜切生菜的细菌生长(P<0.05),且嘉陵20比纳溪效果好。综上所述,桑叶多酚能够延缓鲜切生菜在室温储藏期间的品质劣化,有望成为一种新型的天然鲜切果蔬生物保鲜剂。

关键词：桑叶多酚鲜切生菜抗褐变抗菌生物保鲜剂