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Differential gene expression between asymptomatic HBV carriers and normal adults

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Hepatobiliary & Pancreatic Diseases International 2009年第4期8卷 383-388页

作者： Tang, cui-Lan Chen, zhi Zhejiang Univ Sch Med Affiliated Hosp 1 State Key Lab Diag & Treatment Infect Dis Hangzhou 310003 Zhejiang Peoples R China Xinhua Hosp Zhejiang Prov Dept Liver Dis Hangzhou 310005 Zhejiang Peoples R China

BACKGROUND: There are more than 100 million wa symptomatic HBV carriers (ASCs) in China and they are at a high risk of developing liver disease, which creates a serious health problem. But more than 90% of normal adults clear virus from primary HBV infection, so the difference of gene expression between ASCs and normal adults determines the differential outcomes. To identify differentially expressed genes in ASCs compared to normal adults, we used suppression subtractive hybridization to compare gene expression. METHODS: cDNA subtracted libraries were constructed by suppression subtracted hybridization from peripheral blood monocytes of ASCs and normal adults, the subtracted clones were prescreened by dot blot hybridization, and the levels of genes of interest were confirmed by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: One hundred and two and 96 positive clones were acquired from the A-N and N-A libraries, respectively, and 14 and 11 clones were identified by dot blot hybridization in the A-N and N-A libraries. Two genes were confirmed as differentially expressed between a set of ASC and normal adult samples by real-time RT-PCR. CONCLUSIONS: Differentially expressed genes in peripheral blood mononuclear cells between ASCs and normal adults were isolated by suppression subtractive hybridization, and included some new genes. Of the upregulated genes in ASCs, checkpoint suppressor I is associated with DNA damage-induced cell cycle arrest. Perforin I is associated with inflammation. The information about such alterations in gene expression could be useful for elucidating the mechanisms of ASC pathogenesis.

关键词： hepatitis B virus asymptomatic HBV carriers suppression subtractive hybridization dot blot hybridization reverse transcriptase-polymerase chain reaction

Topological phase transition in cavity optomechanical system with periodical modulation

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Chinese Physics B 2022年第7期31卷 104-111页

作者： zhi-Xu Zhang Lu Qi Wen-Xue cui Shou Zhang hong-Fu Wang Department of Physics College of ScienceYanbian UniversityYanji 133002China School of Physics Harbin Institute of TechnologyHarbin 150001China

We investigate the topological phase transition and the enhanced topological effect in a cavity optomechanical system with periodical *** calculating the steady-state equations of the system,the steady-state conditions of cavity fields and the restricted conditions of effective optomechanical couplings are *** is found that the cavity optomechanical system can be modulated to different topological Su–Schrieffer–Heeger(SSH)phases via designing the optomechanical couplings ***,combining the effective optomechanical couplings and the probability distributions of gap states,we reveal the topological phase transition between trivial SSH phase and nontrivial SSH phase via adjusting the decay rates of cavity ***,we find that the enhanced topological effect of gap states can be achieved by enlarging the size of system and adjusting the decay rates of cavity fields.

关键词： topological phase transition periodical modulation enhanced topological effect

Designer cell-self-implemented labeling of microvesicles in situ with the intracellular-synthesized quantum dots

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Science China Chemistry 2020年第4期63卷 448-453页

作者： Ling-hong Xiong Jia-Wei Tu Ya-Nan Zhang Ling-Ling Yang Ran cui zhi-Ling Zhang Dai-Wen Pang College of Chemistry and Molecular Sciences State Key Laboratory of VirologyInstitute for Advanced Studiesand Wuhan Institute of BiotechnologyWuhan UniversityWuhan 430072China State Key Laboratory of Medicinal Chemical Biology Tianjin Key Laboratoryof Biosensing and Molecular RecognitionResearch Center for Analytical Sciencesand College of ChemistryNankai UniversityTianjin 300071China

Cell-derived microvesicles(MVs) are secreted from almost all kinds of mammalian cells into the extracellular space, and play crucial roles in intercellular communication and transporting biomolecules between cells. However, there is a great challenge for visualizing and monitoring of MVs’ bio-behaviors due to the limitations of existing labeling methods. Herein, we report the first paradigm of designer cell-self-implemented labeling of MVs secreted from living mammalian MCF-7 cells in situ using the intracellular-synthesized fluorescent quantum dots(QDs) during the formation of MVs. By elaborately coupling intracellular biochemical reactions and metabolism pathways, the MCF-7 cells can be illuminated brightly by intracellular-biosynthesized fluorescent CdSe QDs. Simultaneously, intracellular-synthesized QDs can be in situ encapsulated by the secreted MVs budding from the plasma membrane of the fluorescing cells to label the MVs with an efficiency of up to 89.9%. The whole labeling process skillfully combines designer precise cell-tuned intricate synthesis of CdSe QDs with mild in-situ labeling via cell-selfimplementation just after feeding the cell with suitable chemicals, which is structure-or function-nondestructive and much more straightforward and milder than those by chemical conjugation or indirect encapsulation with conventional fluorogenic labels.

关键词： in situ labeling live cell synthesis cell-derived microvesicles quantum dot cell-self-implementation

A Novel Cholesterol Oxidase Biosensor Based on Pt-nanoparticle/Carbon Nanotube Modified Electrode

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Chinese Chemical Letters 2005年第8期16卷 1081-1084页

作者： Qiao cui SHI Tu zhi PENG Department of Chemistry Xixi Campus Zhejiang University Hangzhou 310028

A Pt-nanooarticle/carbon nanotube modified graphite electrode immobilized with cholesterol oxidase/sol＇-gel layer was developed for monitoring *** this electrode,cholesterol concentration（4.0×10^-6 tp 1.0×10^-4mol/L）could be determined accurately in the presence of ascorbic or uric acid,and the response time was rapid （〈 20 s）. This biosensor has high sensitivity and selectivity.

关键词： Pt- nanoparticle carbon nanotube cholesterol oxidase biosensor.

On Interest-Rate Risk in Use of Postal Savings Funds

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The Journal of China Universities of Posts and Telecommunications 2006年第2期13卷 99-102页

作者： DU Chong-dong ZHOU zhi-cui Finance Department Shijiazhuang Post and Telecommunication Technical College Shijiazhuang 050021 P.R. China

This paper analyzes interest-rate risks that postal savings bureau may face. Our analysis is based on the cash flow characteristics of postal savings funds. The analyzed interest-rate risks consist of re-pricing risks, reinvestment risks, yield curve risks, basis risks and other derivative risks such as credit risks and liquidity risks. This paper presents alternative methods to control and manage these interest-rate risks.

关键词： interest rate interest-rate risk postal savings funds

Mitigating corrosion of deposit-covered carbon steel in solution containing sulfate-reducing bacteria by purging nitrogen

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Journal of Iron and Steel Research International 2023年第9期30卷 1879-1887页

作者： Zheng-rong Ye Jie Yang Zheng-bin Wang Xiang Zhou Wei-dong Jiang Ran Yi Xiao-dong cui zhi-hong Zhao Research Institute of Petroleum Exploration and Development PetroChinaBeijing 100083China CAS Key Laboratory of Nuclear Materials and Safety Assessment Institute of Metal ResearchChinese Academy of SciencesShenyang 110016LiaoningChina School of Materials Science and Engineering University of Science and Technology of ChinaHefei 230026AnhuiChina

The corrosion behavior of a carbon steel covered by sand deposits in the solution containing sulfate-reducing bacteria(SRB)under nitrogen purging conditions was studied by electrochemical measurements and surface analyses,aiming at clarifying the mitigation effects of nitrogen purging on SRB-involved under-deposit *** electrochemical results show that the variation trend of polarization resistance with immersion time is unchanged,but the corrosion current density is the lowest in the presence of SRB and deposits under nitrogen purging conditions,as compared to that without purging nitrogen,which are confirmed by the cross-sectional corrosion morphology observation after removing the sand ***,it is found that there are no positive synergistic effects between SRB and deposits on corrosion under nitrogen purging conditions,corresponding to the corrosion *** mitigation on corrosion can be attributed to the less amount of SRB-produced H2S exhausted by purging nitrogen,which is supported by X-ray photoelectron spectrometry ***,purging nitrogen to the solution could be a practically effective way to mitigate the corrosion of deposit-covered carbon steels in SRB-containing solutions.

关键词： Carbon steel Deposit Sulfate-reducing bacteria Nitrogen purging Corrosion mitigation

Clustering of Cardiovascular Risk Factors and Diabetes： A Prospective Cohort Study on the Inner Mongolian Population in China

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Biomedical and Environmental Sciences 2018年第10期31卷 749-756页

作者： WANG Ting Ting LIN Bo cui Wen Xiu ZHANG Ming zhi ZHANG Yong hong ZHANG Shao Yan Department of Epidemiology School of Public Health andJiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases Medical College of Soochow University Suzhou 215123 Jiangsu China Harbin Center for Disease Control and Prevention Harbin 150000 Heilongjiang China

Objective To evaluate the effect of clustering of cardiovascular risk factors（CVRFs） on type 2 diabetes mellitus（T2 DM） incidence and identify some high predictive clusters in the Inner Mongolian population in China. Methods A total of 1,884 Mongolian individuals aged 20 years or above were followed up from 2002 to 2013 and included in the final analysis. We categorized the participants into two subgroups according to the study outcome event. A Cox proportional hazards model was used to evaluate the effect of clustering of CVRFs on the incidence of T2 DM. Areas under the curve were used to compare the effect of every cluster on T2 DM and identify those having higher predictive value. Results We found 203 persons with T2 DM. Subjects with incident T2 DM tended to be older, had a higher prevalence of drinking, had higher systolic and diastolic pressures; total cholesterol, triglyceride, low-density lipoprotein cholesterol, and C-reactive protein levels; waist circumference; body mass index; and heart rate and lower HDL-C level than did those without T2 DM. The multivariable adjusted hazard ratio（95% confidence interval） of T2 DM was calculated based on comparisons with subjects with 0 CVRFs; in participants with 2 and ≥ 3 factors, the adjusted hazard ratios were 2.257（1.448, 3.518） and 3.316（2.119, 5.188）, respectively. Conclusion The clustering of CVRFs increased the risk of T2 DM. On the basis of fast heart rate, the cluster of abdominal obesity and other CVRFs had higher predictive value for T2 DM than the other three CVRF clusters.

关键词： Type 2 diabetes Cardiovascular risk factors Abdominal obesity Heart rate

Risk Assessment of Highways Affected by Debris Flows in Wenchuan Earthquake Area

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Journal of Mountain Science 2013年第2期10卷 173-189页

作者： cui Peng XIANG Ling-zhi ZOU Qiang Institute of Mountain Hazards and Environment/Key Laboratory of Mountain Hazards and Earth Surface Process Chinese Academy of Sciences Chongqing Jiaotong University Graduate University Chinese Academy of Sciences College of Environmental and Resource Southwest University of Science and TechnologyChina

Since the Wenchuan earthquake in China on May 12th, 2008, highways in earthquake-affected areas have been frequently interrupted by debris flows. We analyzed the hazard effect modes and damage processes along highways and developed three key indexes, scale of debris flows, deposits on highways and river blockage, to describe quantitatively the highway disasters. By combining the empirical methods and the actual terrain conditions, we proposed new methods to determine the value of hazard indexes. In addition, we used the economic value and resistance of highway as vulnerability assessment indexes, then determined the specific subindexes for the subgrade, bridges and culverts, and developed a way for the quantified vulnerability zoning. Moreover, we proposed the assessment and mapping methods for highway risk. The risk is described into 5 grades： extremely low risk, low risk, middle risk, high risk and extremely high risk. We applied these methods in a case study carried out on provincial highway S3o3 from Yingxiu Town to Wolong Town, in Wenchuan County. Analysis of debris flow risk for the whole highway, showed that the total length of highway in extremely low risk area was 28.26 km, 4.83 km in low risk area, 8.0 km in middle risk area, 3.65 km in high risk area, and 3.06 km in extremely high risk area. The assessment results are consistent with the field survey data which reflected the disaster situation. This risk method can be used objectively to evaluate the debris-flow risk along highways, and is useful for highway reconstruction in mountainous areas suffering from active debris flows.

关键词： Debris flow Highway disaster Hazardanalysis Vulnerability analysis Risk analysis Wenchuan earthquake

Depth-resolved numerical model of dam break mud flows with Herschel-Bulkley rheology

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Journal of Mountain Science 2022年第4期19卷 1001-1017页

作者： TANG Jin-bo LIN Peng-zhi cui Peng State Key Laboratory of Hydraulics and Mountain River Engineering Sichuan UniversityChengdu 610065China Key Laboratory of Mountain Hazards and Earth Surface Processes Chinese Academy of SciencesInstitute of Mountain Hazards and EnvironmentChinese Academy of Sciences and Ministry of Water ConservancyChengdu 610041China Institute of Geographic Sciences and Natural Resources Research Chinese Academy of SciencesBeijing 100101China

Mud flows are common phenomena in mountainous areas,which can threaten human safety and cause property losses under certain extreme *** the dynamic characteristics of mud flows,especially in the vertical direction,is helpful for risk reduction and hazard *** this study,a 2D depth-resolved numerical model based on Herschel-Bulkley rheology was developed to study the vertical structures of unsteady mud flows with a *** numerical model was solved by the projection method,and the free surface of mud flows was captured through the VOF *** fully validate this new model,a series of laboratory experiments involving dam break mud flows were conducted,and the mud flow heights,bottom pressures and envelopes of mud residuum were *** numerical model proposed in this study was first validated by the steady-state solution for uniform flows of Herschel-Bulkley fluid on an inclined ***,the simulated and measured mud flow heights,bottom pressures at different x locations and envelopes with different bed slopes showed good ***,the numerical results for a Herschel-Bulkley fluid dam break flow were used to validate the proposed model,which further revealed good *** that,the scenarios in which mud flows impact on a structure were numerically studied,and the vertical profiles of the front velocity and impact pressure on the structure were analyzed and *** results show that a plug layer was formed in the mud flow under unsteady and nonuniform flow conditions,and the impact pressure on the structure was dominated by the dynamic *** addition,the vertical position with the maximum impact pressure acting on the structure was not at the bottom or the surface of the mud flows,and the normalized vertical position rose as the yield stress and consistency coefficient increase for Herschel-Bulkley fluids.

关键词： Mud flows Herschel-Bulkley rheology Depth-resolved model Numerical simulation Vertical profiles