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Effects of different concentrations of nicotinamide on hematopoietic stem cells cultured in vitro

World Journal of Stem Cells 2024年第2期16卷 163-175页

作者： yan Ren yan-Ni cui hong-Wei Wang The Second Clinical Medical College Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina Department of Hematology The Second Hospital of Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina Department of Hematology Key Laboratory of Molecular Diagnosis and Treatment of Blood Diseases in Shanxi ProvinceTaiyuan 030001Shanxi ProvinceChina

BACKGROUND In vitro expansion to increase numbers of hematopoietic stem cells(HSCs)in cord blood could improve clinical efficacy of this vital ***(NAM)can promote HSC expansion ex vivo,but its effect on hematopoietic stem and progenitor cells(HSPCs,CD34^(+)CD38)and functional subtypes of HSCs-shortterm repopulating HSCs(ST-HSCs,CD34^(+)CD38CD45RACD49f^(+))and long-term repopulating HSCs(LT-HSCs,CD34^(+)CD38CD45RACD49f^(+)CD90^(+))is not yet *** a sirtuin 1(SIRT1)inhibitor,NAM participates in regulating cell adhesion,polarity,migration,proliferation,and ***,SIRT1 exhibits dual effects by promoting or inhibiting differentiation in different tissues or *** propose that the concentration of NAM may influence proliferation,differentiation,and SIRT1 signaling of *** To evaluate the effects and underlying mechanisms of action of different concentrations of NAM on HSC proliferation and *** CD34^(+)cells were purified from umbilical cord blood using MacsCD34 beads,and cultured for 10-12 d in a serum-free medium supplemented with cytokines,with different concentrations of NAM added according to experimental *** cytometry was used to detect phenotype,cell cycle distribution,and apoptosis of the cultured ***-time polymerase chain reaction was used to detect the transcription levels of target genes encoding stemness-related factors,che mokines,components of hypoxia pathways,and antioxidant ***-dihydro-fluorescein diacetate probes were used to evaluate intracellular production of reactive oxygen species(ROS).Determination of the effect of different culture conditions on the balance of cytokine by cytometric bead *** Compared with the control group,the proportion and expansion folds of HSPCs(CD34^(+)CD38)incubated with 5 mmol/L or 10 mmol/L NAM were significantly increased(all P<0.05).The ST-HSCs ratio and fold expansion of the 5 mmol/L NAM group were significantly

关键词： Hematopoietic stem cells Nicotinamide Concentration Proliferation Differentiation Sirtuin 1

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CO_2浓度加倍下水氮耦合对黄瓜叶片碳氮代谢及其关键酶活性的影响

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植物生理学报 2017年第9期53卷 1717-1727页

作者：李曼董彦红崔青青张文东艾希珍刘彬彬李清明山东农业大学园艺科学与工程学院山东泰安271018 作物生物学国家重点实验室山东泰安271018 农业部黄淮海设施农业工程科学观测实验站山东泰安271018

以‘津优35号’黄瓜(Cucumis sativus)为试材,采用三裂式裂区试验设计,研究了CO_2浓度加倍下水氮耦合对黄瓜叶片碳、氮代谢及其关键酶活性的影响。主区因素为CO_2浓度,设大气CO_2浓度(400μmol·mol^(-1))和加倍CO_2浓度[(800±20)μmol... 详细信息

以‘津优35号’黄瓜(Cucumis sativus)为试材,采用三裂式裂区试验设计,研究了CO_2浓度加倍下水氮耦合对黄瓜叶片碳、氮代谢及其关键酶活性的影响。主区因素为CO_2浓度,设大气CO_2浓度(400μmol·mol^(-1))和加倍CO_2浓度[(800±20)μmol·mol^(-1)]两个水平;裂区因素为水分处理,设无干旱胁迫(灌水上限为田间持水量的95%)和干旱胁迫(灌水上限为田间持水量的75%)两个水平;再裂区因素为施氮量,设施氮量450 kg·hm-2(低氮)和900 kg·hm^(-2)(高氮)两个水平。结果表明:(1)CO_2浓度加倍提高了干旱和高氮条件下黄瓜的株高,且使高氮下的叶面积显著增加。(2)CO_2浓度加倍显著提高了黄瓜叶片蔗糖、淀粉、还原糖含量,蔗糖合成酶(SS)的活性显著增加;高氮显著增加了CO_2浓度加倍条件下SS和蔗糖磷酸合成酶(SPS)活性,促进蔗糖积累和淀粉分解。干旱胁迫使SS活性显著增加,SPS活性显著降低,CO_2浓度加倍显著提高了干旱胁迫下SS和SPS活性以及蔗糖和还原糖含量,且SS和SPS活性以及蔗糖含量随氮素的增加而显著增加。(3)CO_2浓度加倍显著提高了硝酸还原酶(NR)活性,但是谷氨酰胺合成酶(GS)和谷氨酸合酶(GOGAT)活性并未同步增加。在CO_2浓度加倍条件下,高氮使干旱胁迫的黄瓜叶片NR和GOGAT活性显著提高,GS活性的降低得到缓解。(4)加倍CO_2浓度、无干旱胁迫、高氮处理下产量最高,其次是加倍CO_2浓度、干旱胁迫、高氮处理。在大气CO_2浓度和干旱条件下,低氮处理产量最大,继续增施氮肥产量无显著差异,而在CO_2浓度加倍和干旱条件下,增施氮肥黄瓜产量则显著上升。因此,在CO_2浓度加倍条件下高氮促进了干旱胁迫的光合产物转化,使氮同化能力的降低得到缓解或改变,促进干旱胁迫下黄瓜叶片的碳氮代谢,能在一定程度上缓解干旱胁迫造成的不利影响,从而提高黄瓜生长量和产量,可为设施黄瓜CO_2施肥及水分亏缺等逆境下水氮科学管理提供理论依据和技术参数。

关键词：黄瓜 CO2浓度加倍水氮耦合碳氮代谢

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MET receptor tyrosine kinase promotes the generation of functional synapses in adult cortical circuits

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Neural Regeneration Research 2025年第5期20卷 1431-1444页

作者： Yuehua cui Xiaokuang Ma Jing Wei Chang Chen Neha Shakir Hitesch Guirram Zhiyu Dai Trent Anderson Deveroux Ferguson Shenfeng Qiu Basic Medical Sciences University of Arizona College of Medicine-PhoenixPhoenixAZUSA Department of Medicine University of Arizona College of Medicine-PhoenixPhoenixAZUSA

Loss of synapse and functional connectivity in brain circuits is associated with aging and neurodegeneration,however,few molecular mechanisms are known to intrinsically promote synaptogenesis or enhance synapse *** have previously shown that MET receptor tyrosine kinase in the developing cortical circuits promotes dendritic growth and dendritic spine *** investigate whether enhancing MET in adult cortex has synapse regenerating potential,we created a knockin mouse line,in which the human MET gene expression and signaling can be turned on in adult(10–12 months)cortical neurons through doxycycline-containing *** found that similar to the developing brain,turning on MET signaling in the adult cortex activates small GTPases and increases spine density in prefrontal projection *** findings are further corroborated by increased synaptic activity and transient generation of immature silent *** MET signaling resulted in an increasedα-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid/N-methyl-Daspartate(AMPA/NMDA)receptor current ratio,indicative of enhanced synaptic function and *** data reveal that enhancing MET signaling could be an interventional approach to promote synaptogenesis and preserve functional connectivity in the adult *** findings may have implications for regenerative therapy in aging and neurodegeneration conditions.

关键词： aging circuit connectivity cortical circuits molecular mechanisms neural regeneration neurodegeneration synapses

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Building of lightweight Nb_(2)CT_(x) MXene@Co nitrogen-doped carbon nanosheet arrays@carbon fiber aerogels for high-efficiency electromagnetic wave absorption in X and Ku bands inspired by sea cucumber

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Nano Research 2024年第11期17卷 9261-9274页

作者： Jiatong yan Ce cui Wenhao Bai hong Tang Ronghui Guo College of Biomass Science and Engineering Sichuan University Chengdu 610065 China Yibin Industrial Technology Research Institute Sichuan University Yibin 644005 China High-Tech Organic Fibers Key Laboratory of Sichuan Province Chengdu 610041 China Aviation Key Laboratory of Science and Technology on Structural Corrosion Prevention and Control China Special Vehicle Research Institute Jingmen 448035 China Graduate School of Energy Science Kyoto University Kyoto City 606-8501 Japan

The problems of electromagnetic wave(EMW)pollution in X and Ku bands(8–18 GHz)are becoming more and more ***,it is urgent to design EMW absorbing materials with high-efficiency such as thin thickness,lightweight,wide bandwidth and strong EMW *** by the biomorph of sea cucumber,Nb_(2)CT_(x) MXene@Co nitrogen-doped carbon nanosheet arrays@carbon fiber aerogels(Nb_(2)CT_(x)@Co-NC@CFA,Nb_(2)CT_(x)=niobium carbide)were constructed by self-assembly,in-situ chemical deposition and subsequent *** carbon fiber aerogel,as the basic skeleton of sea cucumber,forms lightweight three-dimensional interconnected conductive network,enhances the dielectric loss and extends the multiple reflection and absorption paths of *** the tentacles of sea cucumber surface,Nb_(2)CT_(x) MXene and Co nitrogen-doped carbon nanosheet arrays exist rich heterogeneous interfaces,which play an important role in improving EMW polarization loss and optimizing impedance *** minimum reflection loss(RLmin)of Nb_(2)CT_(x)@Co-NC@CFA reaches−54.7 dB at 9.84 GHz(2.36 mm)with a low filling ratio of 10 wt.%and the effective absorption bandwidth(EAB)of Nb_(2)CT_(x)@Co-NC@CFA reaches 2.96 GHz(8.48–11.44 GHz)with 2.36 mm and 5.2 GHz(12.8–18 GHz)with 1.6 mm,covering most of X and Ku bands by adjusting *** radar cross section(RCS)value of Nb_(2)CT_(x)@Co-NC@CFA is 26.64 dB·m^(2),which is lower than that of the perfect electrical conductor(PEC),indicating that Nb_(2)CT_(x)@Co-NC@CFA can effectively decrease the probability of the target being detected by the radar *** work provides ideas for design and development of EMW absorbing materials with high-efficiency EMW absorption in X and Ku bands.

关键词： bionic sea cucumber Nb_(2)CT_(x)MXene Co nitrogen-doped carbon nanosheet arrays carbon fiber aerogel lightweight electromagnetic wave absorption

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AIM Study on the Reaction of CH2SH Radical with Fluorine Atom

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Chinese Journal of Chemical Physics 2008年第2期21卷 123-130页

作者： cui-hong Suna yan-li Zeng Ling-peng Meng Shi-jun Zheng Institute of Computational Quantum Chemistry Hebei Normal University Shijiazhuan& 050016 China Chemistry and Chemical Engineering College Shijiazhuang University Shijiazhuang 050035 China

The reaction of CH2SH radical with fluorine atom was studied at the levels of B3LYP/6-311G（d,p） and MP2（Full）/6-311G（d,p）. The computational results show that the reaction has three channels and proceeds by the addition of fluorine atoms on carbon or sulfur sites of CH2SH, forming initial intermediates. The calculated results show that the channel, in which fluorine attaches to the carbon atom to form CH2S and HF, is the most likely reaction pathway. Topological analysis of electron density was carried out for the three channels. The change trends of the chemical bonds on the reaction paths were discussed. The energy transition states and the structure transition regions （states） of the three channels were found. The calculated results show that the structure transition regions are broad in unobvious exothermic reactions or unobvious endothermic reactions, and are narrow in obvious exothermic reactions or obvious endothermic reactions.

关键词： Radical reaction Energy transition state Structure transition state Structure transition region Topological analysis of electronic density

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天然锰矿对水体中磷的去除研究

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武汉理工大学学报 2012年第4期34卷 76-80页

作者：王丽丽黄永炳胡涵洪翠妍黄倩董婧涂书新龚文琪李晔武汉理工大学资源与环境工程学院武汉430070 华中农业大学资源与环境学院武汉430070

研究了天然锰矿对水体中磷的去除效果,考察了温度、光照、锰矿用量、溶液pH及共存离子对除磷效果的影响。结果表明,pH对磷的吸附影响很大。离子影响中,Ca2+、Mg2+、CO32-、HCO3-、SiO32-和SO24-的影响不大,而AsO43-和AsO3-却能明显抑制... 详细信息

研究了天然锰矿对水体中磷的去除效果,考察了温度、光照、锰矿用量、溶液pH及共存离子对除磷效果的影响。结果表明,pH对磷的吸附影响很大。离子影响中,Ca2+、Mg2+、CO32-、HCO3-、SiO32-和SO24-的影响不大,而AsO43-和AsO3-却能明显抑制锰矿对磷的吸附。Freundlich等温吸附模型(r2=0.984 4)能很好的描述磷的吸附。动力学数据符合二级动力学模型(r2=0.999 9),最大吸附量为30.40μg/g。FTIR和SEM结果显示磷主要是通过与锰矿表面的羟基发生置换或生成内层络合物而被吸附。天然锰矿,具有较大的比表面积,强选择性,是一种很有前景的去除天然水体中磷的吸附剂。

关键词：天然锰矿磷吸附等温线动力学

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Analysis of the extremely cold and heavy snowfall in North America in January 2015

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Atmospheric and Oceanic Science Letters 2016年第2期9卷 75-82页

作者： cui hong-yan QIAO Fang-li First Institute of Oceanography State Oceanic Administration Key Laboratory of Marine Science and Numerical Modeling State Oceanic Administration

With global warming and declining Arctic sea ice area in autumn observed by satellites since 1979, anomalous cold snaps in recent winters have affected large parts of North America, Europe, and East Asia. In January 2015, North America suffered extremely cold and heavy snowfall events. As revealed in this paper, the NCEP reanalysis data show that the temperature decreased significantly in January 2015 in North America, including the air temperature in the troposphere and the surface air temperature. Moreover, snow cover increased obviously in January 2015 in North America, while there was a significant negative anomaly of geopotential height. The wind formed the anomalous pattern, which favored cold currents blowing to the North American continent from the polar region, and bringing plenty of water vapor. Our results suggest that the anomalous north wind and decreasing westerly jet stream, which allowed cold and moist air to easily penetrate the North American continent from the Arctic, was one of the main reasons for the extremely cold and heavysnow winter of 2015 in North America.

关键词： Snowfall cold winter atmospheric circulation geopotential height strong north wind

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Treatment of gastric carcinoma with lymphoid stroma by immunotherapy:A case report

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World Journal of Clinical Cases 2022年第25期10卷 8962-8967页

作者： Yu-Jie cui yan-yan Ren hong-Zhen Zhang Department of Oncology Hebei General HospitalShijiazhuang 050051Hebei ProvinceChina Department of Medicine Hebei North UniversityZhangjiakou 075000Hebei ProvinceChina

BACKGROUND Gastric cancer with lymphoid stroma(GCLS)is a rare type of gastric cancer characterized by abundant lymphocytic infiltration of the *** is an EpsteinBarr virus-associated gastric cancer with a better prognosis than typical gastric cancer but with similar *** diagnosis is based on pathological,histological and immunohistochemical examination and there are no standardized guidelines for *** SUMMARY This case report describes a 72-year-old man with a 6-mo history of abdominal *** revealed ulcerative lesions in the stomach and gastric cancer was suspected.A preoperative endoscopic biopsy indicated undifferentiated carcinoma and postoperative pathological,histological and immunohistochemical analyses of the resected specimen confirmed a final diagnosis of *** The patient showed high programmed cell death-ligand 1 expression and recovered well after immunotherapy.

关键词： Lymphoid stroma Gastric cancer Epstein-Barr virus Programmed cell death-ligand 1 Immunotherapy Prognosis Case report

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Properties of New Holomorphic Mappings with Respect to Conic Domains

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Acta Mathematica Sinica,English Series 2021年第6期37卷 971-991页

作者： yan yan cui Yong hong XIE He Ju yanG Yu Ying QIAO College of Mathematics and Statistics Zhoukou Normal UniversityZhoukou 466001P.R.China College of Mathematics and Information Science Hebei Normal UniversityShijiazhuang 050024P.R.China College of Science Hebei University of Science and TechnologyShijiazhuang 050024P.R.China

This paper is mainly about holomorphic mappings associated with conic regions which are closely connected with k-ST(α).We introduce new subclasses of starlike(spirallike)functions,namely,S^(p)_(c)(k,α)(S^(p)_(c)(k,α,β)),and discuss their coefficient estimates and the Fekete–Szego–Goluzin’s *** we generalize S^(p)_(c)(k,α,β)on the unit ball B^(n) in C^(n),that is,k-conic spirallike mappings of typeβand orderα.We obtain the growth,covering and distortion theorems of the generalized *** that,we construct k-conic spirallike mappings of typeβand orderαon B^(n) through S_(c)(k,α,β)by the generalized Roper-Suffridge extension operators.

关键词： Holomorphic mappings coefficient estimates growth theorems Roper-Suffridge operators

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绿原酸对中性粒细胞功能及胞外诱捕网形成的影响及机制

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中国药理学通报 2025年第1期41卷 101-107页

作者：杨运刘娅王翠彭春红贵州中医药大学第二临床医学院贵州贵阳550025 七星关区中医妇幼集团医院贵州毕节551700 毕节市中医医院贵州毕节551700 贵州医科大学临床医学院贵州贵阳550025 贵州省人民医院呼吸与危重医学科贵州贵阳550002 国家卫健委肺脏免疫性疾病重点实验室贵州贵阳550002

目的探究绿原酸(chlorogenic acid,CGA)对中性粒细胞(neutrophil,NEU)功能及中性粒细胞胞外诱捕网(neutrophil extracellular traps,NETs)形成的影响及机制。方法从小鼠骨髓中分离并纯化NEU;将NEU与CGA共培养,通过CCK-8法筛选共培养适合的干预浓度;检测NEU迁移能力、髓过氧化物酶(MPO)以及脱颗粒弹性蛋白酶(NE)、基质金属蛋白酶9(MMP-9)的水平观察CGA对NEU功能的影响;免疫荧光法观察NETs的主要结构组蛋白H3及DNA骨架,以及检测NEU释放的游离DNA(cf-DNA)含量,观察NETs的形成情况;检测NEU产生的活性氧(ROS)水平,以确定CGA是否影响NEU释放NETs过程中的ROS的生成。结果CGA在浓度为100、200、400μmol·L^(-1)时对NEU活性有明显抑制作用(P<0.01)。与空白组(Blank)相比,CGA(100、200、400μmol·L^(-1))干预后,细胞迁移能力及MPO、NE、MMP-9分泌量增加(P<0.05),组蛋白H3及DNA骨架、cf-DNA的释放量明显增加(P<0.01),细胞产生的ROS也明显增加(P<0.01),上述作用在一定范围内具有浓度依赖性。结论CGA能够增强NEU功能及促进NETs的形成,可能与促进细胞内ROS水平增加有关。

关键词：绿原酸中性粒细胞胞外诱捕网免疫抗菌活性氧

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