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Effect of basic fibroblast growth factor(bFGF) on the treatment of exposure of the orbital implants

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第9期8卷 620-625页

作者： CUI hong-guang li hui-yan Department of Ophthalmology the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310003 China

Objective: To evaluate the efficacy and the indication of basic fibroblast growth factor (bFGF) in the treatment of exposure of orbital implants. Design: Retrospective and observational case series. Methods: We reviewed 41 patients (41 eyes) suffering exposure of orbital implants from Jan. 2000 to June 2006. The study group patients with mild exposure received com-bined treatment with bFGF and antibiotic drops, and while the control group patients with mild exposure were treated with anti-biotic drops only. The study group patients with moderate and severe exposure received combined treatment with bFGF and antibiotic drops, and after 2 months they were subjected to amniotic membrane transplantation, while the control group patients with moderate and severe exposure underwent amniotic membrane transplantation after using antibiotic drops. Observation of the growth of conjunctival epithelium and comparison of the healing rate of the two groups. Results: The healing rates of the mild, moderate and severe exposure study group were 100% and 92.3%. The healing rates of the mild, moderate and severe exposure control group were 55.6% and 66.7% respectively. The difference of the healing rates of the mild exposure study group and the control group was significant (P=0.033). And the difference of the healing rates of the moderate and severe exposure study group and the control group was not significant (P=0.167). Conclusion: bFGF may promote obviously the healing of orbital implant exposure, particularly it can be the first choice for the treatment of mild degree exposure. For the moderate and severe cases, it can be administered before surgical repair to enhance neovascularization and will tend to increase the success rate of surgical repair.

关键词： Exposure Orbital implants Basic fibroblast growth factor (bFGF)

Effect of dopamine on bone morphogenesis protein-2 expression in human retinal pigment epithelium

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International Journal of Ophthalmology(English edition) 2017年第9期10卷 1370-1373页

作者： hong-hui li Yan-li Sun Dong-Mei Cui Juan Wu Jun-Wen Zeng Chengdu University of TCM Chengdu 610075Sichuan ProvinceChina Ophthalmology Center Yinzhou Hospital Affiliated to Medical School of Ningbo UniversityNingbo 315040Zhejiang ProvinceChina State Key Laboratory of Ophthalmology Zhongshan OphthalmicCenterSun Yat-sen UniversityGuangzhou 510060Guangdong ProvinceChina

AIM：To investigate the effect of dopamine on bone morphogenesis protein-2（BMP-2）expression in retinal pigment epithelium（RPE）cells in ***：ARPE-19 cells as a human RPE cell line were cultured with dopamine for different times（2,4,6,8,12,16and 24h）or with different concentrations（0.1,1,2,5,10,20,and 100μg/m L）in ***-2 m RNA expression level in ARPE-19 cells was analyzed with real-time polymerase chain reaction（PCR）analysis and BMP-2 protein level was measured with Western blot *** active form of BMP-2 in the culture medium was measured with enzymelinked immunosorbent assay（EliSA）.RESULTS：The expression level of BMP-2 increased significantly cultured with 20μg/m L dopamine,at different time points（P〈0.05）.BMP-2 m RNA level peaked 2h and the protein level peaked at 6 and 8h after *** concentrations of secreted BMP-2 elevated at 12h and peaked at 24h（P〈0.05）in a time-dependent *** with 100μg/m L dopamine for 6h,the expression levels of BMP-2 m RNA and protein in ARPE-19 cells were enhanced significantly compared to that in the untreated cells（P〈0.05）.And secreted BMP-2 protein in the cell culture supernatant was also increased（P〈0.05）.CONCLUSION：Dopamine up-regulate BMP-2 expression in RPE cells,and this may be associated with its inhibitive effect on myopia development.

关键词： dopamine retinal pigmental epithelieum bone morphogenetic protein-2

Regulation of scleral fibroblast differentiation by bone morphogenetic protein-2

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International Journal of Ophthalmology(English edition) 2014年第1期7卷 152-156页

作者： hong-hui li li-Jun Huo Zhen-Ya Gao Feng Zhao Jun-Wen Zeng State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-Sen University

Bone morphogenesis proteins(BMPs) are multi-functional growth factors. They are expressed in retina,retinal pigment epithelium(RPE) and sclera and serve as a regulator in the growth and development of the eye. This article reviewed the chondrogenic potency of the sclera,biochemical and pathological changes of myopic scleral tissue and the differentiation of chondrogenesis by BMP-2. We proposed the hypothesis that BMP-2 can regulate differentiate of scleral fibroblasts and affect the development of myopia.

关键词： bone morphogenetic protein-2 sclera myopia

All-trans retinoic acid increases ARPE-19 cell apoptosis via activation of reactive oxygen species and endoplasmic reticulum stress pathways

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International Journal of Ophthalmology(English edition) 2020年第9期13卷 1345-1350页

作者： Juan Wu Zhen-Ya Gao Dong-Mei Cui hong-hui li Jun-Wen Zeng State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou 510060Guangdong ProvinceChina Xuchang University School of MedicineXuchang 461000Henan ProvinceChina Chengdu Aier Eye Hospital Chengdu 610000Sichuan ProvinceChina

AIM:To explore the apoptosis of ARPE-19 cells after the treatment with different doses of all-trans-retinoic acid(ATRA).METHODS:ARPE-19 cells were used in the in-vitro *** cytometry assay was employed to evaluate the level of reactive oxygen species(ROS)and *** effects of ATRA(concentrations from 2.5 to 20μmol/L)on the expression of endoplasmic reticulum stress(ERS)markers in vitro were evaluated by Western blot and realtime quantitative polymerase chain reaction(qRT-PCR)*** contribution of ROS and ERS-induced apoptosis in vitro was determined by using N-acetyl-L-cysteine(NAC)and Salubrinal,an antagonist of NAC and ERS,***:Flow cytometry showed that ATRA significantly increased ARPE-19 cell apoptosis and ROS levels in each group(F=86.39,Pling pathways.

关键词： all-trans-retinoic acid retinal pigment epithelium apoptosis reactive oxygen species endoplasmic reticulum stress

Anatomical retroperitoneoscopic retroperitoneal lymph node dissection for clinical stage I nonseminomatous germ cell tumors： initial operative experience

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Asian Journal of Andrology 2014年第1期16卷 136-139页

作者： Kai Yao Zai-Shang li Fang-Jian Zhou Zi-Ke Qin Zhuo-Wei liu Yong-hong li hui Han Department of Urology Cancer Center Sun Yat-Sen University State Key Laboratory of Oncology in South China Collaborative Innovation Center of Cancer Medicine Guangzhou China.

To introduce the technique of anatomical retroperitoneoscopic retroperitoneal lymph node dissection （ARRPLND） was performed in 12 consecutive patients with a clinical stage I nonseminomatous germ-cell tumor （NSGCT） between February 2008 and October 2010. All procedures were performed using a modified template nerve-sparing approach. The retroperitoneal space was adequately expanded using double gasbags. After the retroperitoneal fat was cleared, two relatively bloodless planes were entered consecutively to expose the lymph node and permit dissection. Dissection proceeded first in the plane between the anterior renal fascia and posterior peritoneum, and secondly in the avascular plane between the posterior renal fascia and transversalis fascia. The proximal spermatic vein was clipped at the initial stage. En bloc resection of the lymph tissue and fat between the anterior renal fascia and posterior renal fascia were performed. Three patients （25%） had pathologic stage IIA disease and received adjuvant chemotherapy. No recurrence was observed during follow-up ranging from 26 to 58months. The median operative time was 205min （range： 165-430min） and median estimated blood loss was 320ml （range： 100-1200ml）. There were two intraoperative complications （Clavien grade II） and one open conversion due to perforation of the peritoneum. Postoperative complications （Clavien I） developed in three patients. Normal antegrade ejaculation recovered by 1 month following the operation. Our preliminary results indicate that ARRPLND is technically feasible and associated with satisfactory clinical outcomes for clinical stage I NSGCT. Further studies are necessary to evaluate this technique.

关键词： nonseminomatous retroperitoneoscopic retroperitoneal lymph node testicular cancer

High-continuity genome assembly of the jellyfish Chrysaora quinquecirrha

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Zoological Research 2021年第1期42卷 130-134页

作者： Wang-Xiao Xia Hao-Rong li Jing-Hao Ge Yao-Wu liu hong-hui li Yan-Hua Su Hai-Zhen Wang hui-Fang Guo Yu-Xuan Dai Yao-Wen liu Xing-Chun Gou Shaanxi Key Laboratory of Brain Disorders Institute of Basic Translational MedicineXi’an Medical UniversityXi’anShaanxi 710021China Key Laboratory of Animal Gene Editing and Animal Cloning in Yunnan Province Yunnan Agricultural UniversityKunmingYunnan 650201China Center for Ecological and Environmental Sciences Northwestern Polytechnical UniversityXi’anShaanxi 710072China ZhiQiao Research Institute ChangshaHunan 410000China Shaanxi Key Laboratory of Infection and Immune Disorders School of Basic Medical ScienceXi’an Medical UniversityXi’anShaanxi 710021China

DEAR EDITOR,The Atlantic sea nettle(Chrysaora quinquecirrha)has an important evolutionary position due to its high ecological ***,due to limited sequencing technologies and complex jellyfish genomic sequences,the current *** genome assembly is highly ***,we used the most advanced high-throughput chromosome conformation capture(Hi-C)technology to obtain high-coverage sequencing data of the *** *** then anchored these data to the previously published contig-level assembly to improve the ***,a high-continuity genome sequence of *** was successfully assembled,which contained 1882 scaffolds with a N50 length of 3.83 *** N50 length of the genome assembly was 5.23 times longer than the previously released one,and additional analysis revealed that it had a high degree of genomic continuity and *** of the high-continuity genome sequence of *** not only provides a basis for the study of jellyfish evolution through comparative genomics but also provides an important resource for studies on jellyfish growth and development.

关键词： fish continuity assembly

Ab initio calculations of mirror energy difference in sd-shell nuclei

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Chinese Physics C 2023年第12期47卷 73-95页

作者：李红蕙李健国谢萌冉左维 Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China School of Nuclear Science and Technology University of Chinese Academy of SciencesBeijing 100049China

Mirror energy difference is a key observable in isospin symmetry breaking,containing rich information about nuclear *** the mechanisms underlying mirror energy difference is important in nuclear *** the present work,we extensively investigated mirror energy difference using ab initio valence-space inmedium similarity renormalization group approach,focusing specifically on sd-shell *** low-lying spectra of Al isotopes and N=8 isotones,together with their mirror nuclei,were calculated,followed by a systematic analysis of the evolution of the mirror energy *** results suggest that the large mirror energy difference is mainly caused by the weakly-bound effects and large average occupations of the 1s1/2 ***,we compare the results of our ab initio calculations with shell model results,elucidating the relationship and coherence between these two models.

关键词： ab initio isospin symmetry breaking Thomas Erhman shift

Safety study on HIC containing waste resin with respect to hydrogen release

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Nuclear Science and Techniques 2019年第4期30卷 35-43页

作者： Shuai-Wei Zhao Mei-Lan Jia Xing-Qian Jiao Meng-Qi Qiu hong-hui li Yu-Chen liu liang Mao Wei liu Dong liang China Institute for Radiation Protection Sichuan Environmental Protection and Engineering CO. LTD. CNNC

To explore the behavior of radiolytically produced hydrogen release from the waste resin stored in a high integrated container(HIC), and the mechanism of hydrogen diffusion in a near-surface disposal facility, both experimental studies and numerical simulations were performed through an accelerated irradiation test and simulated disposal, respectively. Results indicated that,100 years after disposal, the highest hydrogen concentration appeared in the cell where the HICs were placed. The volume fraction for different scenarios postulated in the numerical simulation was 2.64% for Scenario 1, 2.28% for Scenario 2, and 3.965% for Scenario 3, all of which are lower than the hydrogen explosion limit of 4.1%. The results indicated that the simulated HIC disposal scheme is safe.

关键词： Radioactive waste resin High integrated container Repository Radiolysis Hydrogen release

Pretreatment with interleukin-33 reduces warm hepatic ischemia/ reperfusion injury in mice

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Chinese Medical Journal 2013年第10期126卷 1855-1859页

作者： li Shu ZHU Feng-xue ZHANG hong-bin li hui AN You-zhong Department of Critical Care Medicine Peking University People'sHospital Beijing 100044 China

Background Interleukin （IL）-33 is a recently identified member of the IL-1 family that binds to the receptor, ST2L. This study examined IL-33 production in mouse liver and investigated its role in hepatic ischemia/reperfusion （I/R） injury. Methods Male BALB/c mice （（22＋3） g） were subjected to 90 minutes partial hepatic ischemia, followed by 6 hours reperfusion. First, mice were randomized into two groups： control group （laparotomy only, without blocking blood supply） and ischemia model group. IL-33 mRNA and serum protein levels were measured at 30, 60, 90 minutes after ischemia and 2 and 6 hours after reperfusion. Second, mice were randomized into four groups： control, model （injection of rabbit IgG polyclonal antibody）, recombinant IL-33 intervention and anti-ST2L antibody intervention group. Mice were sacrificed 6 hours after reperfusion. liver pathology was observed via transmission electron microscopy. Serum aspartate aminotransferase （AST）, alanine aminotransferase （ALT）, IL-4, IL-5, IL-13, interferon-y （IFN-y） and tumor necrosis factor-a （TNF-a） levels were measured. Results Levels of IL-33 mRNA and protein did not change during ischemia （P 〉0.05） but increased significantly during reperfusion （P 〈0.05）. After reperfusion for 6 hours, serum levels of ALT, AST, IL-4, IL-5, IL-13, IFN-~ and TNF-a were significantly increased （P 〈0.05）, and hepatocellular ultrastructure was damaged. Pretreatment with IL-33 attenuated severity of liver damage compared with controls, but pretreatment with anti-ST2L antibody increased severity. Serum levels of IL-4, IL-5 and IL-13 protein increased whereas IFN-y decreased following IL-33 pretreatment. Pretreatment with anti-ST2L antibody significantly decreased serum IL-4, IL-5, IL-13 levels and increased serum IFN-r levels compared with controls （P 〈0.05）. There was no change in the level of TNF-a. Conclusion IL-33 is produced systematically and locally in liver during I/R injury. Pretreatment with IL-33 is therapeutic for hepatic

关键词： liver ischemia/reperfusion T helper lymphocyte,interleukin-33