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ROLE OF B LYMPHOCYTE AND ITS SUBPOPULATIONS IN PATHOGENESIS OF IMMUNORELATED PANCYTOPENIA

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Chinese Medical Sciences Journal 2007年第3期22卷 199-202页

作者： Rong Fu Zong-hong Shao hong Liu Yu-hong wu Hua-quan Wang Li-min Xing Department of Hematology and Oncology General Hospital of Tianjin Medical University Tianjin 300052

Objective To measure the quantities and apoptosis-related protein levels of B lymphocyte in the patients with immunorelated pancytopenia （IRP） and explore the action of B lymphocyte in the pathogenic mechanism of IRP. Methods Quantifies of whole B lymphocytes and CD5^＋ B lymphocytes as well as the expressions of Fas and Bcl-2 in B lymphocytes in 35 patients with untreated IRP, 15 IRP patients in complete remission （CR）, and 10 normal controls were assayed by flow cytometry. The percentages of B lymphocyte and CD5^＋ B lymphocyte were significantly higher in untreated IRP patients than in CR IRP patients and normal controls （ P 〈 0. 05 ）, and there was no significant difference between the latter two groups （ P 〉 0. 05 ）. There was no significant difference of Fas expression in B lymphocyte among three groups （ P 〉 0. 05）. The expression of Bcl-2 in B lymphocyte was significantly higher in untreated patients than in CR patients or normal controls （ P 〈 0. 01 ）, and significantly higher in CR patients than in normal controls （ P 〈 0. 01 ）. The apoptosis. related index was significantly lower in untreated patients than in CR patients or normal controls （ P 〈 0. 05 ）, and signif. icantly lower in CR patients than in normal controls （ P 〈 0. 05 ）. The percentage of B lymphocyte was positively correlated with post-treated response time （ r = 0. 53, P 〈 0. 01 ）. Conclusion The production of auto-antibodies in IRP patients probably has some relationship with the abnormal quantifies of B lymphocyte and its subpopulations as well as with the inhibition of B lymphocyte apoptosis.

关键词： immunorelated pancytopenia B lymphocyte quantifies apoptosis

Endogenous Hydrogen Peroxide Plays a Positive Role in the Upregulation of Heme Oxygenase and Acclimation to Oxidative Stress in Wheat Seedling Leaves

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Journal of Integrative Plant Biology 2009年第10期51卷 951-960页

作者： Xiao-Yue Chen Xiao Ding Sheng Xu Ren Wang Wei Xuan Ze-Yu Cao Jian Chen hong-hong wu Mao-Bing Ye Wen-Biao Shen College of Life Sciences Cooperative Demonstration Laboratory of Centrifuge Technique Nanjing Agricultural University and Beckman Coulter Ltd. Co. Nanjing Agricultural University Nanjing 210095 China Institute of Botany Jiangsu Province and the Chinese Academy of Sciences (Mem. Sun Yat-Sen) Jiangsu Province Key Laboratory for Plant Ex-situ Conservation Nanjing 210014 China

Pretreatment of lower H2O2 doses （0.05, 0.5 and 5 mM） for 24 h was able to dose-dependently attenuate lipid peroxidation in wheat seedling leaves mediated by further oxidative damage elicited by higher dose of H2O2 （150 mM） for 6 h, with 0.5 mM H2O2 being the most effective concentrations. Further results illustrated that 0.5 mM H2O2 pretreatment triggered the biphasic production of H2O2 during a 24 h period. We also noticed that only peak Ⅰ （0.25 h） rather than peak Ⅱ （4 h） was approximately consistent with the enhancement of heme oxygenase （HO） activity, HO-1 gene expression. Meanwhile, enhanced superoxide dismutase （SOD） activity, Mn-SOD and Cu, Zn-SOD transcripts might be a potential source of peak Ⅰ of endogenous H2O2. Further results confirmed that 0.5 mM H2O2 treatment for 0.5 h was able to upregulate HO gene expression, which was detected by enzyme activity determination, semi-quantitative reverse transcription-polymerase chain reaction and western blotting. Meanwhile, the application of N,N＇-dimethylthiourea, a trap for endogenous H2O2, not only blocked the upregulation of HO, but also reversed the corresponding oxidation attenuation. Together, the above results suggest that endogenous H2O2 production （peak Ⅰ） plays a positive role in the induction of HO by enhancing its mRNA level and protein expression, thus leading to the acclimation to oxidative stress.

关键词： acclimation to oxidative stress endogenous hydrogen peroxide heme oxygenase signal transduction Triticum

Molecular mechanism of modulating the liquefaction epididymal protease inhibitor of human semen

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Asian Journal of Andrology 2008年第5期10卷 770-775页

作者： Zeng-Jun Wang Wei Zhang Ning-Han Feng Ning-hong Song hong-Fei wu Yuan-Geng Sui Laboratory of Reproductive Medicine Department of Urology the First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China

Aim： To study the molecular mechanism of epididymal protease inhibitor （Eppin） modulating the process of prostate specific antigen （PSA） digesting semenogelin （Sg）. Methods： Human Sg cDNA （nucleotides 82-849） and Eppin cDNA （nucleotides 70-423） were generated by polymerase chain reaction （PCR） and cloned into pET-100D/TOPO. Recombinant Eppin and Sg （rEppin and rSg） were produced by BL21 （DE3）. The association of Eppin with Sg was studied by far-western immunoblot and radioautography. In vitro the digestion of rSg by PSA in the presence or absence of rEppin was studied. The effect of anti-Q20E （N-terminal） and C-terminal of Eppin on Eppin-Sg binding was monitored. Results： Eppin binds Sg on the surface of human spermatozoa with the C-terminal of Eppin （amino acids 75-133）. rSg was digested with PSA and many low molecular weight fragments were produced. When rEppin is bound to rSg, then digested by PSA, incomplete digestion and a 15-kDa fragment results. Antibody binding to the N-terminal of rEppin did not affect rSg digestion. Addition of antibodies to the C-terminal of rEppin inhibited the modulating effect of rEppin. Conclusion： Eppin protects a 15-kDa fragment of rSg from hydrolysis by PSA.

关键词： epididymal protease inhibitor, semenogelin prostate specific antigen

Sky location of massive black hole binaries in the foreground of Galactic white dwarf binaries

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 97-108页

作者： Pan Guo hong-Bo Jin Cong-Feng Qiao Yue-Liang wu The International Centre for Theoretical Physics Asia-Pacific University of Chinese Academy of Sciences The School of Physical Sciences University of Chinese Academy of Sciences Institute for Frontiers in Astronomy and Astrophysics Beijing Normal University National Astronomical Observatories Chinese Academy of Sciences Institute of Theoretical Physics Chinese Academy of Sciences

For space-based gravitational wave （GW） detection,the main noise source for massive black hole binaries （MBHBs） is attributed to approximately 10～7double white dwarf binaries in the *** a GW source,the amplitude of the detector response,recorded by a space-based gravitational wave detector,exhibits a modulation effect with a year period when observing the source from various orbital *** the adverse conditions mentioned above,where there is a strong foreground noise and annual modulation in the signals,we employed the wavelet transform and the strong-amplitude relevant orbital position search methods,which allows the weak MBHB sources to achieve higher locating *** detail,for two MBHB sources of lower intensity,the precision of luminosity distance,represented by the ratio ?DL/DLat the 95%confidence level,is enhanced by factors of ～*** angular resolutions,denoted by??s,are enhanced by a factor of ～*** improvements increase the number of detectable GW sources,facilitate multi-messenger follow-up observations and provide constraints on the cosmological constant.

关键词： space-based gravitational wave detection gravitational waves massive black hole binaries

A Study of the Development of the World Postal Parcel Service

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The Journal of China Universities of Posts and Telecommunications 2003年第3期10卷 107-112页

作者： ZHANG hong fen, wu hong bin, YANG Wen xiu (Training Department of Shijiazhuang Postal College, Shijiazhuang 050021, P. R. China) Training Department of Shijiazhuang Postal College Shijiazhuang 050021

The paper makes an analysis of the market demands, market competition,collaboration and the current development of the postal parcel service, and discusses some factorscounteracting the development of the postal parcel service. Furthermore, this paper gives anorientation to the future development of the postal parcel service through a comprehensive researchon the developing trend of the postal parcel service in some countries.

关键词： the market of postal parcel service service structure individual service market orientation

Regulation and function of endoplasmic reticulum autophagy in neurodegenerative diseases

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Neural Regeneration Research 2025年第1期20卷 6-20页

作者： Xiu-Yun Zhao De-En Xu Ming-Lei wu Ji-Chuan Liu Zi-Ling Shi Quan-hong Ma Department of Neurology and Clinical Research Center of Neurological Disease the Second Affiliated Hospital of Soochow UniversitySuzhouJiangsu ProvinceChina Institute of Neuroscience&Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases Soochow UniversitySuzhouJiangsu ProvinceChina Department of Neurology Jiangnan University Medical CenterWuxiJiangsu ProvinceChina

The endoplasmic reticulum,a key cellular organelle,regulates a wide variety of cellular *** reticulum autophagy,one of the quality control systems of the endoplasmic reticulum,plays a pivotal role in maintaining endoplasmic reticulum homeostasis by controlling endoplasmic reticulum turnover,remodeling,and *** this review,we briefly describe the endoplasmic reticulum quality control system,and subsequently focus on the role of endoplasmic reticulum autophagy,emphasizing the spatial and temporal mechanisms underlying the regulation of endoplasmic reticulum autophagy according to cellular *** also summarize the evidence relating to how defective or abnormal endoplasmic reticulum autophagy contributes to the pathogenesis of neurodegenerative *** summary,this review highlights the mechanisms associated with the regulation of endoplasmic reticulum autophagy and how they influence the pathophysiology of degenerative nerve *** review would help researchers to understand the roles and regulatory mechanisms of endoplasmic reticulum-phagy in neurodegenerative disorders.

关键词： autophagy endoplasmic reticulum endoplasmic reticulum autophagy endoplasmic reticulum quality control system endoplasmic reticulum receptors endoplasmic reticulum-associated degradation neurodegeneration neurodegenerative disease selective autophagy unfolded protein response

基于两级遗传算法的多工厂供应链批量计划问题

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上海交通大学学报 2003年第4期37卷 473-478页

作者：杨红红吴智铭上海交通大学自动化系上海200030

研究了分布式多工厂协作生产的供应链的约束批量计划问题 .以最小化项目的生产成本、库存成本与运输成本之和为目标 ,建立了加工能力受限情况下的数学规划模型 .通过分解问题模型 ,提出了基于两级遗传算法优化的求解策略 .对典型算例进... 详细信息

研究了分布式多工厂协作生产的供应链的约束批量计划问题 .以最小化项目的生产成本、库存成本与运输成本之和为目标 ,建立了加工能力受限情况下的数学规划模型 .通过分解问题模型 ,提出了基于两级遗传算法优化的求解策略 .对典型算例进行了仿真 ,并与分枝定界法的求解结果进行了比较 .结果表明 ,此方案是有效的、可行的 .

关键词：分布式多工厂供应链约束批量计划遗传算法

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模糊需求环境下多工艺批量生产计划

上海交通大学学报 2002年第8期36卷 1121-1126,1137页

作者：杨红红吴智铭上海交通大学自动化研究所上海200030

基于模糊集理论与容差法思想 ,研究了具有模糊客户需求与多工艺约束的批量生产计划问题 ,采用遗传算法寻找优化的计划方案 .在遗传算法中 ,提出了分级构造初始种群的策略 ,按问题的多约束特征对交叉算子与变异算子重新进行了定义 ,并对... 详细信息

基于模糊集理论与容差法思想 ,研究了具有模糊客户需求与多工艺约束的批量生产计划问题 ,采用遗传算法寻找优化的计划方案 .在遗传算法中 ,提出了分级构造初始种群的策略 ,按问题的多约束特征对交叉算子与变异算子重新进行了定义 ,并对不同模糊参数和优化目标下的批量计划进行了定性和定量评估 .结果表明 ,在一定的需求满意度下以最大化生产利润为优化目标 ,不仅制造商可以获得较为理想的生产利润值。

关键词：批量生产计划模糊客户需求多生产工艺约束遗传算法模糊集理论容差法制造业

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动态柔性制造系统仿真平台

上海交通大学学报 2002年第8期36卷 1109-1112,1117页

作者：王胤杨红红庞小红吴智铭上海交通大学自动化系上海200030

针对通常柔性制造系统 ( FMS)仿真方法在体现制造系统动态变化特性方面的不足 ,开发了一种 FMS的可视化、分布式仿真平台 .该平台模拟真实 FMS的运行 ,较好地体现了离散事件动态系统的特性 ,可以作为调度、控制算法的测试平台 .作为示... 详细信息

针对通常柔性制造系统 ( FMS)仿真方法在体现制造系统动态变化特性方面的不足 ,开发了一种 FMS的可视化、分布式仿真平台 .该平台模拟真实 FMS的运行 ,较好地体现了离散事件动态系统的特性 ,可以作为调度、控制算法的测试平台 .作为示例 ,在该平台上成功地实现了一种基于遗传算法的 FMS动态调度算法 ,解决了

关键词：动态柔性制造系统仿真平台系统仿真动态调度算法 Petri网系统设计