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Phosphatidic Acid （PA） PP2A Activity and PIN1 Binds PP2AA1 to Regulate Polar Localization

Molecular Plant 2013年第5期6卷 1692-1702页

作者： hong-bo gao yu-jia chu hong-wei xue National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chineseacademy of Sciences 200032 Shanghai People's Republic of China

Phospholipase D （PLD） exerts broad biological functions in eukaryotes through regulating downstream effectors by its product, phosphatidic acid （PA）. Protein kinases and phosphatases, such as mammalian target of rapa- mycin （mTOR）, Protein Phosphatase 1 （PP1） and Protein Phosphatase 2C （PP2C）, are PA-binding proteins that execute crucial regulatory functions in both animals and plants. PA participates in many signaling pathways by modulating the enzymatic activity and/or subcellular localization of bound proteins. In this study, we demonstrated that PLD-derived PA interacts with the scaffolding A1 subunit of Protein Phosphatase 2A （PP2A） and regulates PP2A-mediated PIN1 dephos- phorylation in Arabidopsis. Genetic and pharmacological studies showed that both PA and PP2A participate in the regu- lation of auxin distribution. In addition, both the phosphorylation status and polar localization of PIN1 protein were affected by PLD inhibitors, Exogenous PA triggered the membrane accumulation of PP2AA1 and enhanced the PP2A activity at membrane, while PLD inhibition resulted in the reduced endosomal localization and perinuclear aggregation of PP2AA1. These results demonstrate the important role of PLD-derived PA in normal PP2A-mediated PIN dephosphoryl- ation and reveal a novel mechanism, in which PA recruits PP2AA1 to the membrane system and regulates PP2A function on membrane-targeted proteins. As PA and PP2A are conserved among eukaryotes, other organisms might use similar mechanisms to mediate multiple biological processes.

关键词： phosphatidic acid (PA) PP2A PIN1 phosphorylation polar localization.

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Acquired methemoglobinemia in a third trimester puerpera and her premature infant with sodium nitrite poisoning:A case report

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World Journal of Clinical Cases 2024年第22期12卷 5151-5158页

作者： Dong-Sheng Fei Yang gao xue-jiao Bao yu-jia Tang Yi-Lu Lin jia-Xi Xu jia-Ning Zhang bo-Wen Liu Kai Kang Department of Critical Care Medicine The First Affiliated Hospital of Harbin Medical UniversityHarbin 150001Heilongjiang ProvinceChina Department of Critical Care Medicine The Sixth Affiliated Hospital of Harbin Medical UniversityHarbin 150027Heilongjiang ProvinceChina

BACKGROUND The common cause of sodium nitrite poisoning has shifted from previous accidental intoxication by exposure or ingestion of contaminated water and food to recent alarming intentional intoxication as an employed method of suicide/*** subsequent formation of methemoglobin(MetHb)restricts oxygen transport and utilization in the body,resulting in functional hypoxia at the tissue *** clinical practice,a mismatch of cyanotic appearance and oxygen partial pressure usually contributes to the identification of *** recognition of characteristic mismatch and accurate diagnosis of sodium nitrite poisoning are prerequisites for the implementation of standardized systemic *** SUMMARY A pregnant woman was admitted to the Department of Critical Care Medicine at the First Affiliated Hospital of Harbin Medical University due to consciousness disorders and drowsiness 2 h before ***,she developed vomiting and cyanotic *** woman underwent orotracheal intubation,invasive mechanical ventilation(IMV),and correction of internal environment disturbance in the *** premature infant was born with a higher-than-normal MetHb level of 3.3%,and received detoxification with methylene blue and vitamin C,supplemental vitamin K1,an infusion of fresh frozen plasma,as well as respiratory support via orotracheal intubation and *** day 3 after admission,the puerpera regained consciousness,evacuated the IMV,and resumed enteral *** was then transferred to the maternity ward 24 h *** day 7 after admission,the woman recovered and was discharged without any *** MetHb can cross through the placental *** of MetHb both reflects severity of the sodium nitrite poisoning and serves as feedback on therapeutic effectiveness.

关键词： Sodium nitrite poisoning Methemoglobinemia Methemoglobin Third trimester puerpera Premature infant Placental barrier Methylene blue Case report

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Frontiers in high entropy alloys and high entropy functional materials

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Rare Metals 2024年第10期43卷 4639-4776页

作者： Wen-Tao Zhang Wen Huang Fu-Jie yu Qi Zhao Xin-Dong Zhu Tao Feng Yao jiang Xiao-Ming Li xue-Fei Miao He Zhu Si Lan xue-Qian Wang Bao-Long Shen Zhe jia Feng-Qi Zhang wei Wang Qi Liu Yang Ren Xun-Li Wang Xiao-Ya Cui Xiao-Yang Wang Ya-Nan Chen Bing-Bing Fan Meng-Ru Li Qi-Long gao jia-Ming Guo Zeng-Bao jiao Zhi-Min Guo yu-Ping Zhao Qian yu Rui Huang Ling-Kun Zhang Yi-Ping Lu Xu-bo Li Huai-Jun Lin Yan Ma wei-Ming Yang Zhi-Hua Shen Hai-Yan He Yong-Gang Sun Yong-jiang Huang De-Zhuang Wang Qing-Feng Liu Fei-Yang Wang Hui Liu hong-Hui Wu Li-Qiang Wang Yang Lu Nan Wang Tian-Li Wang wei-Bing Liao Yi-Han Wang yuan Wu yu-Zhen Yin Jie Pan Yi Zhang Qing Wang jian-Yang Zhang Tao Yang Yasir Sohail Jin-yu Zhang Hua Ji Qiang Li wei-Di Liu Zhi-Gang Chen Xiong-Jun Liu Wen Ma Liang Mao Zhen-Duo Wu School of Materials Science and Engineering Nanjing University of Science and Technology jiangsu Key Laboratory for Advanced Metallic Materials School of Materials Science and Engineering Southeast University Department of Physics City University of Hong Kong School of Materials Science and Engineering Tianjin University School of Physics and Microelectronics Zhengzhou University Department of Mechanical Engineering Research Institute for Advanced Manufacturing The Hong Kong Polytechnic University Center of Electron Microscopy and State Key Laboratory of Silicon Materials School of Materials Science and Engineering Zhejiang University Key Laboratory of Solidification Control and Digital Preparation Technology (Liaoning Province) School of Materials Science and Engineering Dalian University of Technology Institute of Advanced Wear & Corrosion Resistance and Functional Materials Jinan University School of Mechanics and Civil Engineering China University of Mining and Technology School of Physical Sciences Great Bay University School of Materials Science andEngineering Harbin Institute of Technology State Key Laboratory of Materials-Oriented Chemical Engineering College of Chemical Engineering Nanjing Tech University Beijing Advanced Innovation Center for Materials Genome Engineering Research Institute for Carbon Neutrality University of Science and Technology Beijing Department of Mechanical Engineering The University of Hong Kong School of Computing Macquarie University Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University State Key Laboratory for Advanced Metals and Materials University of Science and Technology Beijing State Key Laboratory of Materials Processing and Die & Mould Technology School of Materials Science and Engineering Huazhong University of Science and Technology Institute of materials Shanghai University Department of Materials Science and Engineering C

Owing to their exceptional properties, high-entropy alloys(HEAs) and high-entropy materials have emerged as promising research areas and shown diverse applications. Here,the recent advances in the field are comprehensively reviewed,organized into five sections. The first section introduces the background of HEAs, covering their definition, significance,application prospects, basic properties, design principles, and microstructure. The subsequent section focuses on cutting-edge high-entropy structural materials, highlighting developments such as nanostructured alloys, grain boundary engineering,eutectic systems, cryogenic alloys, thin films, micro-nano-lattice structures, additive manufacturing, high entropy metallic glasses, nano-precipitate strengthened alloys, composition modulation, alloy fibers, and refractory systems. In the following section, the emphasis shifts to functional materials, exploring HEAs as catalysts, magneto-caloric materials, corrosion-resistant alloys, radiation-resistant alloys, hydrogen storage systems, and materials for biomedicine. Additionally, the review encompasses functional high-entropy materials outside the realm of alloys, including thermoelectric, quantum dots, nanooxide catalysts, energy storage materials, negative thermal expansion ceramics,and high-entropy wave absorption materials. The paper concludes with an outlook, discussing future directions and potential growth areas in the field. Through this comprehensive review,researchers, engineers, and scientists may gain valuable insights into the recent progress and opportunities for further exploration in the exciting domains of high-entropy alloys and functional materials.

关键词： Cutting-edge structural materials Cutting-edgefunctional materials High entropy alloys

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The In-Flight Realtime Trigger and Localization Software of GECAM

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Research in Astronomy and Astrophysics 2024年第10期24卷 11-24页

作者： Xiao-yun Zhao Shao-Lin Xiong Xiang-Yang Wen Xin-Qiao Li Ce Cai Shuo Xiao Qi Luo Wen-Xi Peng Dong-Ya Guo Zheng-Hua An Ke Gong Jin-yuan Liao Yan-Qiu Zhang yue Huang Lu Li Xing Wen Fei Zhang Jing Duan Chen-wei Wang Dong-Li Shi Peng Zhang Qi-Bin Yi Chao-Yang Li Yan-Bing Xu Xiao-Hua Liang Ya-Qing Liu Da-Li Zhang Xi-Lei Sun Fan Zhang Gang Chen Huan-yu Wang Sheng Yang Xiao-Jing Liu Min gao Mao-Shun Li Jin-Zhou Wang Xing Zhou Yi Zhao Wang-Chen xue Chao Zheng jia-Cong Liu Xing-bo Han Jin-Ling Qi jia Huang Ke-Ke Zhang Can Chen Xiong-Tao Yang Dong-Jie Hou yu-Sa Wang Rui Qiao Xiang Ma Xiao-bo Li Ping Wang Xin-Ying Song Li-Ming Song Shi-Jie Zheng Bing Li hong-Mei Zhang yue Zhu wei Chen jian-jian He Zhen Zhang Jin Hou hong-Jun Wang Yan-Chao Hao Xiang-yu Wang Zong-yuan Yang Zhi-Long Wen Zhi Chang yuan-yuan Du Rui gao Xiao-Fei Lan Yan-Guo Li Gang Li Xu-Fang Li Fang-Jun Lu hong Lu Bin Meng Feng Shi Hui Wang Hui-Zhen Wang yu-Peng Xu jia-wei Yang xue-Juan Yang Shuang-Nan Zhang Chao-yue Zhang Cheng-Mo Zhang Zhi-Cheng Tang Cheng Cheng Key Laboratory of Particle Astrophysics Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China University of Chinese Academy of Sciences Chinese Academy of SciencesBeijing 100049China School of Information Science and Technology Southwest Jiaotong UniversityChengdu 611756China Key Laboratory of Stellar and Interstellar Physics and Department of Physics Xiangtan UniversityXiangtan 411105China Physics and Space Science College China West Normal UniversityNanchong 637002China Department of Astronomy Beijing Normal UniversityBeijing 100875China Innovation Academy for Microsatellites of CAS Shanghai 201203China Changchun University of Science and Technology Changchun 130022China Chinese Academy of Sciences South America Center for Astronomy National Astronomical ObservatoriesCASBeijing 100101China

Realtime trigger and localization of bursts are the key functions of GECAM,an all-sky gamma-ray monitor launched on 2020 December *** developed a multifunctional trigger and localization software operating in the CPU of the GECAM Electronic *** onboard software has the following features:high trigger efficiency for real celestial bursts with a suppression of false triggers caused by charged particle bursts and background fluctuation,dedicated localization algorithm optimized for both short and long bursts,and low time latency of the trigger information which is downlinked through the Global Short Message Communication service of the global BeiDou navigation *** paper provides a detailed description of the design and development of the trigger and localization software system for *** covers the general design,workflow,the main functions,and the algorithms used in the *** paper also includes on-ground trigger tests using simulated gamma-ray bursts generated by a dedicated X-ray tube,as well as an overview of the performance for real celestial bursts during its in-orbit operation.

关键词： telescopes instrumentation:detectors methods:observational

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Ins（1,4,5）P3 Suppresses Protein Degradation in Plant Vacuoles by Regulating SNX-Mediated Protein Sorting

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Molecular Plant 2016年第10期9卷 1440-1443页

作者： yu-jia chu Xu Chen hong- wei xue National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant Sciences Shanghai Institute of Plant Physiology and Ecology Chinese Academy of Sciences 300 Fenglin Road Shanghai 200032 China

Dear Editor, Protein degradation is an essential process for protein dynamics and the development of living organisms, and defective protein degradation can lead to abnormalities in protein accumulation and cell functions. In addition to the conserved proteasome- dependent pathway in both mammals and plants, the lytic vacuole-dependent pathway is crucial for protein degradation in plants （Cai et al., 2014）.

关键词： ARABIDOPSIS TRANSPORT COMPLEX

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我国药品集中带量采购政策扩散分析

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中国卫生政策研究 2024年第9期17卷 76-82页

作者：高一博王兆旸吕博赵婧萱谢佳鑫薛咏茜高雨润孟开首都医科大学公共卫生学院北京100069 首都医科大学附属北京天坛医院北京100070

目的:开展我国近年来药品集中带量采购相关的政策扩散分析,为我国药品集中带量采购政策的制定提供参考依据。方法:通过中央及各省级政府官网、卫生健康委员会官网、医疗保障局官网等途径,检索2009年1月1日—2023年12月31日涉及药品集中... 详细信息

目的:开展我国近年来药品集中带量采购相关的政策扩散分析,为我国药品集中带量采购政策的制定提供参考依据。方法:通过中央及各省级政府官网、卫生健康委员会官网、医疗保障局官网等途径,检索2009年1月1日—2023年12月31日涉及药品集中带量采购的政策文件。基于政策扩散理论,采用参照网络分析法分析政策扩散的强度、广度和速度,使用政策关键词时序分析法分析政策的扩散方向。结果:在药品集中带量采购政策发展的两个阶段中,医保治理阶段的政策出台量达到高峰;扩散强度、广度最高的前十项政策均为中央政策,并且多以通知和意见类为主,此外,较新颁布的政策,扩散速度更快;扩散方向上,自上而下和平行扩散两种扩散趋势较为明显。结论:我国药品集中带量采购政策扩散以中央政策为重点,扩散速度逐年上升,建议加强中央与地方的政策协同,建立全国统一的药品集中带量采购信息平台,并优化各级政府之间的学习和竞争机制,发挥“政策试验”的优势。

关键词：药品集中带量采购政策扩散政策网络分析政策时序分析

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响应面法优化超声辅助提取大果沙枣叶多酚工艺及其抗氧化和抗糖化能力研究

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中国调味品 2024年第10期49卷 186-193页

作者：孙妍高尚郭玉婷陈璐薛雪唐辉廖兵武贾瑞博广东海洋大学食品科技学院广东湛江524088 石河子大学药学院新疆石河子832003 塔里木大学食品科学与工程学院新疆阿拉尔843300

在推动循环经济的进程中,该研究着重于大果沙枣叶多酚的提取及其应用潜力。利用超声波辅助提取技术对大果沙枣叶中的多酚类化合物进行了提取工艺的优化,并对其抗氧化和抗糖化能力进行了全面评估。试验确定了最佳提取条件为超声时间20.00... 详细信息

在推动循环经济的进程中,该研究着重于大果沙枣叶多酚的提取及其应用潜力。利用超声波辅助提取技术对大果沙枣叶中的多酚类化合物进行了提取工艺的优化,并对其抗氧化和抗糖化能力进行了全面评估。试验确定了最佳提取条件为超声时间20.00 min、料液比1∶53.40(g/mL)、超声温度69.70℃、超声功率400 W,此条件下得到多酚提取量为(28.24±0.46)mg/g。抗氧化试验表明,该多酚对DPPH·、·OH和ABTS^(+)·具有较强清除能力,其IC_(50)值分别为0.112,0.110,0.146 mg/mL。在BSA-GLC模拟反应体系下,其糖化抑制率显著提高至(44.64±7.20)%。研究结果表明,超声辅助提取的大果沙枣叶多酚具有显著的体外抗氧化和抗糖化能力,其能力随着大果沙枣叶多酚浓度的增加而增强,表明其可作为潜在的天然食品添加剂,为沙枣副产品的综合利用提供了新方向。

关键词：大果沙枣叶多酚超声辅助提取响应面抗氧化美拉德反应

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基于Nrf2/HO-1信号通路探讨补阳还五汤对AngⅡ诱导的H9c2细胞铁死亡的保护作用

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科学技术与工程 2024年第1期24卷 155-162页

作者：薛亚楠王建波曲怡高佳馨张云雨张立德辽宁中医药大学中医药创新工程技术中心沈阳110847 中医脏象理论及应用教育部重点实验室沈阳110847 辽宁中医药大学研究生学院沈阳110847

基于Nrf2/HO-1铁死亡信号通路探讨补阳还五汤对血管紧张素Ⅱ(Angiotensin Ⅱ,AngⅡ)诱导的H9c2大鼠心肌细胞铁死亡的影响。体外培养H9c2心肌细胞,分别设正常组(ZC)、模型组(MX)、补阳还五汤低剂量组(BD)、补阳还五汤中剂量组(BZ)、补阳... 详细信息

基于Nrf2/HO-1铁死亡信号通路探讨补阳还五汤对血管紧张素Ⅱ(Angiotensin Ⅱ,AngⅡ)诱导的H9c2大鼠心肌细胞铁死亡的影响。体外培养H9c2心肌细胞,分别设正常组(ZC)、模型组(MX)、补阳还五汤低剂量组(BD)、补阳还五汤中剂量组(BZ)、补阳还五汤高剂量组(BG)和阳性对照组(YX)。除正常组外,其余各组采用1μmol/L AngⅡ建立心肌细胞损伤模型,同时补阳还五汤低、中、高剂量组分别用含0.5、1、2 mg/mL补阳还五汤的培养基培养,阳性对照组使用含10μmol/L铁死亡抑制剂Ferrostatin-1(Fer-1)的培养基培养,24 h后收集细胞。采用CCK-8法检测各组心肌细胞活性;细胞铁含量检测试剂盒测定细胞内铁含量;免疫荧光染色法检测各组Nrf2蛋白表达;q-PCR法检测各组细胞核转录相关因子2(Nrf2)、血红加氧酶-1(HO-1)、谷胱甘肽过氧化物酶4(GPX4)的mRNA表达水平;ELISA试剂盒检测细胞谷胱甘肽(GSH)和GPX4的蛋白表达水平;Western blot法检测Nrf2和HO-1的蛋白表达。结果表明,补阳还五汤高剂量组可明显提高AngⅡ诱导的H9c2细胞活力下降(P<0.01),减少AngⅡ条件下铁含量(P<0.05);与模型组相比,补阳还五汤高剂量组能显著逆转AngⅡ诱导的H9c2细胞GSH、GPX4、Nrf2和HO-1的基因和蛋白表达水平的下降,使AngⅡ条件下受到抑制的Nrf2表达增加,与阳性对照组结果一致(P<0.01,P<0.05)。补阳还五汤可能通过Nrf2/HO-1信号通路调控心肌细胞铁死亡从而保护高血压继发的心肌损伤。

关键词：补阳还五汤心肌铁死亡高血压

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First light for the sodium laser guide star adaptive optics system on the Lijiang 1.8 m telescope

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Research in Astronomy and Astrophysics 2016年第12期16卷 41-45页

作者： Kai wei Min Li Shan-Qiu Chen Yong bo Feng Chen Jun-wei Zuo Qi Bian Ji Yao Lu-chun Zhou Lin wei Dong-hong Chen Yang gao KaiJin Xiao-Lin Dai Han-chu Fu Chang Xu Zhi-Chao Wang Xiang-Hui xue xue-Wu Chen Xian-Mei Qian yu Zhou Hao Xian Qin-Jun Peng Chang-Hui Rao Zu-Yan Xu yu-Dong Zhang Key Laboratory on Adaptive Optics Chinese Academy of SciencesChengdu 610209China Institute of Optics and Electronics Chinese Academy of SciencesChengdu 610209China Research Center for Laser Physics and Technique Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijing 100190China University of-Science and Technology of China Hefei 230036China Wuhan Institute of Physics and Mathematics Chinese Academy of SciencesWuhan 430071China Anhui Institute of Optics and Fine Mechanics Chinese Academy of SciencesHefei 230031China yunnan Observatories Chinese Academy of SciencesKunming 650200China University of Chinese Academy of Sciences Beijing 100049China

A first generation sodium Laser Guide Star Adaptive Optics System （LGS-AOS） was developed and integrated into the Lijiang 1.8 m telescope in 2013. The LGS-AOS has three sub-systems：（1） a 20W long pulsed sodium laser, （2） a 300-millimeter-diameter laser launch telescope, and （3） a 37-element com- pact adaptive optics system. On 2014 January 25, we obtained high resolution images of an my 8.18 star, HIP 43963, during the first light of the LGS-AOS. In this paper, the sodium laser, the laser launch telescope, the compact adaptive optics system and the first light results will be presented.

关键词： instrumentation: adaptive optics -- turbulence -- atmospheric effects

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Identification of novel genes associated with duck OASL in response to influenza A virus

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Journal of Integrative Agriculture 2019年第7期18卷 1451-1459页

作者： WANG Xiao-xue LU Chang RONG En-guang HU jia-xiang XING Yan-ling LIU Zheng-yu gao chu-ze LIU Jin-hua HUANG Yin-hua State Key Laboratory of Agrobiotechnology China Agricultural UniversityBeijing 100193P.R. China Key Laboratory of Animal Epidemiology and Zoonosis Ministry of Agriculture/College of Veterinary MedicineChina Agricultural UniversityBeijing 100193P.R.China

2′-5′-Oligoadenylate synthetase like protein(OASL) plays a key role in response to viral infections through selectively activating the OAS/RNase L or OASL/RIG-I signaling *** classic pathway of OASL is well-known,its regulated genes or co-actors are largely *** study the possible molecular mechanism of duck OASL(dOASL),we performed RNA-sequencing(RNA-seq) and immunoprecipitation and mass spectrometry(IP-MS) at the level of mRNA and protein,*** RNA-seq,we used DF1 cell lines(DF1 dO ASL+/+,DF1 cO ASL–/–,and DF1) with or without the CK/0513 H5 N1 virus(A/chicken/huabei/0513/2007) infection.1 737 differentially expressed genes(DEGs) were identified as candidate target genes regulated by *** Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG) analysis and weighted Correlation Network Analysis(WGCNA) were *** identified one important yellow co-expression module correlated with antiviral immune *** this module,Ankyrin repeat and FYVE domain containing 1(ANKFY1),harboring a BTB domain similar to the methyl CpG-binding protein 1(MBD1) which bound to OASL in human,was regulated by *** protein level,133 host proteins were ***,ANKFY1 was one of them binding to dOASL *** phylogenomic and chromosomal syntenic analysis demonstrated MBD1 was absent in birds,while mammals *** is suggested that OASL-ANKFY1 interaction might act as a compensatory mechanism to regulate gene expression in *** findings will provide a useful resource for the molecular mechanism research of dOASL.

关键词： duck OASL ANKFY1 compensatory mechanism influenza A virus

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