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Recycling of Solanum Sucrose Transporters Expressed in Yeast, Tobacco, and in Mature Phloem Sieve Elements

Molecular Plant 2010年第6期3卷 1064-1074页

作者： Johannes Liesche hong-xia he Bernhard Grimm Alexander Schulz Christina Kuhn Humboldt University Institute of Biology Plant Physiology Philippstraβe 13 Building 12 10115 Berlin Germany Plant Physiology and Anatomy Laboratory Department of Plant Biology Faculty of Life Sciences Copenhagen University Thorvaldsensvej 40 DK-1871 Frederiksberg C Denmark

The plant sucrose transporter SUT1 （from Solanum tuberosum, S. lycopersicum, or Zea mays） exhibits redoxdependent dimerization and targeting if heterologously expressed in S. cerevisiae （Krtigel et al., 2008）. It was also shown that SUT1 is present in motile vesicles when expressed in tobacco cells and that its targeting to the plasma membrane is reversible. StSUT1 is internalized in the presence of brefeldin A （BFA） in yeast, plant cells, and in mature sieve elements as confirmed by immunolocalization. These results were confirmed here and the dynamics of intracellular SUT1 localization were further elucidated. Inhibitor studies revealed that vesicle movement of SUT1 is actin-dependent. BFA-mediated effects might indicate that anterograde vesicle movement is possible even in mature sieve elements, and could involve components of the cytoskeleton that were previously thought to be absent in SEs. Our results are in contradiction to this old dogma of plant physiology and the potential of mature sieve elements should therefore be re-evaluated. In addition, SUT1 internalization was found to be dependent on the plasma membrane lipid composition. SUT1 belongs to the detergent-resistant membrane （DRM） fraction in planta and is targeted to membrane raft-like microdomains when expressed in yeast （Kr（igel et al,, 2008）, here, SUT1-GFP expression in different yeast mutants, which were unable to perform en- docytosis and/or raft formation, revealed a strong link between SUT1 raft localization, the sterol composition and mem- brane potential of the yeast plasma membrane, and the capacity of the SUT1 protein to be internalized by endocytosis. The results provide new insight into the regulation of sucrose transport and the mechanism of endocytosis in plant cells.

关键词： Transporters membrane proteins phloem physiology protein targeting potato.

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Professor Yongjia Duan: a distinguished plant pathologist and agricultural educator

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Protein & Cell 2019年第11期10卷 779-782页

作者： Fei Du Wei-Ping Deng xia-hong he hong Cai You-Yong Zhu State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan Yunnan Agricultural UniversityKunming 650201China Key Laboratory for Agro-biodiversity and Pest Control of Ministry of Education Yunnan Agricultural UniversityKunming 650201China

Professor Yongjia Duan(段永嘉)is a distinguished plant pathologist and agricultural educator in China he has devoted his entire life in developing and sharing his knowl?edge and experiences in Plant *** was born in 1910 in Siping,Jilin *** 1931,he went to Hokkaido University in Japan to study plant pathology,where he obtained his bachelors *** Japan,he met *** Omura,a primary school teacher(Fig.1).They fell in love and got *** 1937,*** and his wife went back to China,hoping that he could contribute to the development of scienee and technology in China.

关键词： has devoted distinguished

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Correlation between optical coherence tomography, multifocal electroretinogram findings and visual acuity in diabetic macular edema

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International Journal of Ophthalmology(English edition) 2020年第10期13卷 1592-1596页

作者： hong-he xia Jia-Lin Chen Hao-Yu Chen hong-Jie Lin Joint Shantou International Eye Center Shantou University and the Chinese University of Hong KongShantou 515041Guangdong ProvinceChina

AIM:To analyze the correlation between macular morphology and function in eyes with diabetic macular edema(DME).METHODS:Fifty-five eyes with different visual acuity(VA)of 32 patients who suffered from DME were analyzed using multifocal electroretinography(mfERG)and optical coherence tomography(OCT).The parameters of mfERG including implicit times and response amplitude were compared to those of 50 normal eyes of 36 age-matched *** analysis was performed between VA,the parameters of mfERG including implicit times and response amplitude,and the central macular thickness(CMT).RESULTS:The amplitude of N1 and P1 were significantly decreased and their latency were significantly increased in five ring regions of the retina in patients with *** was statistically significant correlation between logMAR BCVA and P1 amplitude densities in rings 1-4(r=-0.306,-0.536,-0.470,-0.362;P=0.023,here was poor correlation between the CMT and best-corrected visual acuity(BCVA;r=0.288,P=0.033),but there was no significant correlation between CMT and amplitude or implicit time of N1 and P1(P>0.05)in the central macular *** stepwise regression analysis showed that P1 amplitude density in ring 2 was the only contributor to the ***:It seems to be more appropriate of combining use of mfERG with OCT for the evaluation of macular function in eyes with DME.

关键词： diabetic macular edema optical coherence tomography multifocal electroretinography best-corrected visual acuity

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Endoscopic findings and pathologic characteristics of gastric eosinophilic granuloma:A report of 18 patients

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World Journal of Gastroenterology 2006年第46期12卷 7542-7546页

作者： Wen-Chong Song Jie-Ping Yu Lei Shen hong xia he-Sheng Luo Division of Gastroenterology Renmin Hospital ofWuhan University Wuhan 430060 Hubei Province China

AIM: To investigate the endoscopic findings and patholo- gic characteristics of gastric eosinophilic granuloma (GEG). METHODS: A retrospective study of 18 cases of gastric eosinophilic granulomas was conducted. Gastroscopy was performed and all specimens of biopsies were stained by H&E and observed under light microscopy. RESULTS: Ulcer was the most frequent endoscopic appearance. The others included deformed pylorus and/or duodenal bulb, esophagitis, mucous hyperemia and/or mucosal erosion. Eosinophilic cell infiltration and generous hyperplasia of arterioles, venules and lymph vessels were found in the lesions of the patients. Interstitium had massive eosinophilic infiltrates and was made up of collagen fibers and fibroblasts. Lymphoid follicles were revealed in some sections of biopsies. CONCLUSION: GEG is lack of specific symptoms and physical signs. It can be misdiagnosed as gastric ulcer in most cases before biopsies. Endoscopy and endoscopic multiple deep biopsies in suspected areas are indispensable for correct diagnosis of GEG.

关键词： Gastric eosinophilic granuloma Endoscopy Pathology

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Synthesis of Tritium and Deuterium Labelled Agmatine

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Chinese Chemical Letters 2006年第5期17卷 587-590页

作者： hong xia he Zhi Bing ZheNG Lan Fu CheN Zhan Bin ZHANG Chun HU Song LI Shenyang Pharmaceutical University Shenyang 110016 Beijing Institute of Pharmacology and Toxicology Beijing 100850 Beijing Normal University Beijing 100875

An efficient procedure for the synthesis of agmatine labelled with tritium and deuterium is reported. The final tritiated product 4 was obtained with a specific activity of 40 Ci/mmol and a radiochemical purity of 95%.

关键词： Agmatine labelled compound synthesis deuterium tritium.

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Nuclear apoptosis induced by isolated mitochondria

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Cell Research 2000年第3期10卷 221-232页

作者： JIANG ZheNG FAN, YUN ZHAO, xia hong, Zhong he ZHAI (College of Life Sciences, Peking University, Beijing, 100871 China) College of Life Sciences Peking University Beijing China

We isolated and purified mitochondria from mouse livers and spinach leaves. When added into egg extracts of Xenopus laevis, they caused nuclei of mouse liver to undergo apoptotic changes. Chromatin condensation, margination and DNA ladder were observed. After incubating isolated mitochondria in some hypotonic solutions, and centrifuging these mixtures at high speed, we got mitochondrial supernatants. It was found that in the absence of cytosolic factor, the supernatant alone was able to induce apoptotic changes in nuclei. The effective components were partly of protein. DNA fragmentation was partly inhibited by caspase inhibitors AC-DEVD-CHO and AC- YVADCHO. Meanwhile, caspase inhibitors fully blocked chromatin condensation. Primary characterization of the nuclear endonuclease(s) induced by mitochondrial supernatants was also conducted. It was found that this endonuclease is different from endonuclease G, cytochrome c-induced nuclease, or Ca2+-activated endonuclease.

关键词： Mitochondria supernatant apoptosis egg ex- tract nuclei DNA cleavage.

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The Potato Sucrose Transporter StSUT1 Interacts with a DRM-Associated Protein Disulfide Isomerase

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Molecular Plant 2012年第1期5卷 43-62页

作者： Undine Krugel hong-xia he Konstanze Gier Jana Reins Izabela Chincinska Bernhard Grimm Waltraud X. Schulze Christina Kuhn Institute of Biology Department of Plant Physiology Humboldt University 10115 Berlin Germany Max Planck Institute of Molecular Plant Physiology Am MGhlenberg 1 14476 Potsdam-Golm Germany

Organization of proteins into complexes is crucial for many cellular functions. Recently, the SUT1 protein was shown to form homodimeric complexes, to be associated with lipid raft-like microdomains in yeast as well as in plants and to undergo endocytosis in response to brefeldin A. We therefore aimed to identify SUTl-interacting proteins that might be involved in dimerization, endocytosis, or targeting of SUT1 to raft-like microdomains. Therefore, we identified potato membrane proteins, which are associated with the detergent-resistant membrane （DRM） fraction. Among the proteins identified, we clearly confirmed StSUT1 as part of DRM in potato source leaves. We used the yeast two-hybrid split ubiq- uitin system （SUS） to systematically screen for interaction between the sucrose transporter StSUT1 and other membrane- associated or soluble proteins in vivo. The SUS screen was followed by immunoprecipitation using affinity-purified StSUTl-specific peptide antibodies and mass spectrometric analysis of co-precipitated proteins. A large overlap was ob- served between the StSUTl-interacting proteins identified in the co-immunoprecipitation and the detergent-resistant membrane fraction. One of the SUTl-interacting proteins, a protein disulfide isomerase （PDI）, interacts also with other sucrose transporter proteins. A potential role of the PDI as escort protein is discussed.

关键词： Protein-protein interactions phloem proteins vesicle trafficking endocytosis.

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2,3-Butanediol from the leachates of pine needles induces the resistance of Panax notoginseng to the leaf pathogen Alternaria panax

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Plant Diversity 2023年第1期45卷 104-116页

作者： Tian-Yao Li Chen Ye Yi-Jie Zhang Jun-Xing Zhang Min Yang xia-hong he Xin-Yue Mei Yi-xiang Liu You-Yong Zhu Hui-Chuan Huang Shu-Sheng Zhu School of Agriculture Yunnan UniversityKunming650500China State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan Yunnan Agricultural UniversityKunming650201China Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China Yunnan Agricultural UniversityKunming650201China Southwest Forestry University Kunming650224China

Compared with the use of monocultures in the field,cultivation of medicinal herbs in forests is an effective strategy to alleviate *** interactions between herbs and trees play an important role in disease suppression in *** evaluated the ability of leachates from needles of Pinus armandii to induce resistance in Panax notoginseng leaves,identified the components via gas chromatography-mass spectrometry(GC-MS),and then deciphered the mechanism of 2,3-Butanediol as the main component in the leachates responsible for resistance induction via RNA sequencing(RNA-seq).Prespraying leachates and 2,3-Butanediol onto leaves could induce the resistance of *** to Alternaria *** RNA-seq results showed that prespraying 2,3-Butanediol onto leaves with or without *** infection upregulated the expression of large number of genes,many of which are involved in transcription factor activity and the mitogen-activated protein kinase(MAPK) signaling ***,2,3-Butanediol spraying resulted in jasmonic acid(JA)-mediated induced systemic resistance(ISR) by activating MYC2 and ***,2,3-Butanediol induced systemic acquired resistance(SAR) by upregulating pattern-triggered immunity(PTI)-and effector-triggered immunity(ETI)-related genes and activated camalexin biosynthesis through activation of ***,2,3-Butanediol from the leachates of pine needles could activate the resistance of *** to leaf disease infection through ISR,SAR and camalexin ***,2,3-Butanediol is worth developing as a chemical inducer for agricultural production.

关键词： Pinus armandii Allelopathy herbs Induce resistance Diversity Leaf disease

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翼状胬肉切除联合自体角膜缘干细胞移植治疗原发性翼状胬肉的Meta分析

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眼科新进展 2010年第4期30卷 361-366页

作者：夏红和严宗辉汕头大学广东省汕头市515041 香港中文大学联合汕头国际眼科中心暨南大学医学院附属深圳市眼科医院广东省深圳市518000

目的通过比较翼状胬肉切除联合自体角膜缘干细胞移植术与其他手术方法治疗原发性翼状胬肉的复发率,寻找一种能有效降低原发性翼状胬肉复发率的手术方法。方法电子检索相关文献,按照排除标准剔除不符合要求的文献。对符合条件的文献按对... 详细信息

目的通过比较翼状胬肉切除联合自体角膜缘干细胞移植术与其他手术方法治疗原发性翼状胬肉的复发率,寻找一种能有效降低原发性翼状胬肉复发率的手术方法。方法电子检索相关文献,按照排除标准剔除不符合要求的文献。对符合条件的文献按对照组手术方式分成4个组,以治疗组和对照组复发率的比值比(oddratio,OR)为统计量,应用RevMan4.2软件进行异质性检验和Meta分析,并评价发表偏倚的影响。结果检索到符合要求的文献24篇共27项研究,均为随机对照试验。Meta分析结果显示:翼状胬肉切除联合自体角膜缘干细胞移植术与对照组比较,复发率的差异均有统计学意义:总的对照组合并OR值(ORs)为0.19,95%CI(0.15,0.25),P<0.01;单纯翼状胬肉切除术组ORs为0.12,95%CI(0.08,0.17),P<0.01;翼状胬肉切除联合自体球结膜移植术组ORs为0.32,95%CI(0.13,0.81),P<0.05;翼状胬肉切除联合羊膜移植术组ORs为0.43,95%CI(0.26,0.71),P<0.01;翼状胬肉切除联合术中使用丝裂霉素C组ORs为0.21,95%CI(0.10,0.45),P<0.01。结论翼状胬肉切除联合自体角膜缘干细胞移植能有效降低原发性翼状胬肉的术后复发率,明显优于其他传统的手术方法。

关键词：原发性翼状胬肉角膜缘干细胞移植 Meta分析

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3D Printing of Tough Hydrogel Scaffolds with Functional Surface Structures for Tissue Regeneration

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Nano-Micro Letters 2025年第2期17卷 18-45页

作者： Ke Yao Gaoying hong Ximin Yuan Weicheng Kong Pengcheng xia Yuanrong Li Yuewei Chen Nian Liu Jing he Jue Shi Zihe Hu Yanyan Zhou Zhijian Xie Yong he State Key Laboratory of Fluid Power and Mechatronic Systems School of Mechanical EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China Liangzhu Laboratory Zhejiang UniversityHangzhou 310027People’s Republic of China Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province College of Mechanical EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China Stomatology Hospital School of StomatologyZhejiang University School of MedicineHangzhou 310027People’s Republic of China State Key Laboratory of Advanced Welding and Joining Harbin Institute of TechnologyHarbin 150001People’s Republic of China Institute of Digital Medicine Nanjing First HospitalNanjing Medical UniversityNanjing 210006People’s Republic of China

Hydrogel scaffolds have numerous potential applications in the tissue engineering ***,tough hydrogel scaffolds implanted in vivo are seldom reported because it is difficult to balance biocompatibility and high mechanical *** by Chinese ramen,we propose a universal fabricating method(printing-P,training-T,cross-linking-C,PTC&PCT)for tough hydrogel scaffolds to fill this ***,3D printing fabricates a hydrogel scaffold with desired structures(P).Then,the scaffold could have extraordinarily high mechanical properties and functional surface structure by cycle mechanical training with salting-out assistance(T).Finally,the training results are fixed by photo-cross-linking processing(C).The tough gelatin hydrogel scaffolds exhibit excellent tensile strength of 6.66 MPa(622-fold untreated)and have excellent ***,this scaffold possesses functional surface structures from nanometer to micron to millimeter,which can efficiently induce directional cell ***,this strategy can produce bionic human tissue with mechanical properties of 10 kPa-10 MPa by changing the type of salt,and many hydrogels,such as gelatin and silk,could be improved with PTC or PCT *** experiments show that this scaffold can effectively promote the new generation of muscle fibers,blood vessels,and nerves within 4 weeks,prompting the rapid regeneration of large-volume muscle loss injuries.

关键词： 3D printing Tough hydrogel scaffold Functional surface structure Tissue regeneration Biomaterials

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